Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,325,833 T→G V363G (GTC→GGC)  yciQ → enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,8330TG98.3% 184.2 / NA 58V363G (GTC→GGC) yciQenhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (31/26);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTT  >  NC_000913/1325612‑1326062
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      
cGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCTCAGAGCGTGTATTACAGGAAGCGTTATTTCGTAAAGGCCATAATGGCGTCCTTActgcga                                                                                                                                                                                                                          >  2:297780/1‑231 (MQ=255)
             ctcGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGCTAAGGGCGTTCTTACGGGGAGACGCAACagag                                                                                                                                                                                                              >  1:446889/1‑235 (MQ=255)
             ctcGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGt                                                                                                                                                                                                             >  1:474865/1‑236 (MQ=255)
                gATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCtt                                                                                                                                                                                                          >  1:118115/1‑236 (MQ=255)
                 aTGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGGTCTTACGTGGAGACGCAAAAGAGTCTTa                                                                                                                                                                                                         >  2:723446/1‑236 (MQ=255)
                   gCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCTGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACg                                                                                                                                                                                                       <  2:118115/236‑1 (MQ=255)
                                 cgacgaTAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGTGATAAAGGCGTTCTTACGGGGAGACGCACCAGAGACTTACGCAGGGCATTTTTAc                                                                                                                                                                                         >  2:685781/1‑236 (MQ=255)
                                  gacgaTAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACg                                                                                                                                                                                        >  2:588276/1‑236 (MQ=255)
                                        aaaGGTATGTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCa                                                                                                                                                                                  <  2:170100/236‑1 (MQ=255)
                                               tttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                                                                                                                                           >  2:133452/1‑236 (MQ=255)
                                               tttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAttt                                                                                                                                                                           >  1:566910/1‑236 (MQ=255)
                                                ttGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGGGAGGCAACAGAGGCTTACGCAGGGCATTTTTTCGGATGCAGAAAttgt                                                                                                                                                                          >  2:11387/1‑234 (MQ=255)
                                                   tgCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGAGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTAt                                                                                                                                                                        >  2:444108/1‑235 (MQ=255)
                                                        atatCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAAtt                                                                                                                                                                            >  1:156853/1‑226 (MQ=255)
                                                            cGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTa                                                                                                                                                              >  1:592709/1‑236 (MQ=255)
                                                                     gATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGc                                                                                                                                                                                                 >  1:490268/1‑192 (MQ=255)
                                                                     gATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGc                                                                                                                                                                                                 <  2:490268/192‑1 (MQ=255)
                                                                             aaGGAAAAATTCATCTGGAAGATCAGTCGGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTAc                                                                                                                                             <  2:585663/236‑1 (MQ=255)
                                                                                     aTTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGa                                                                                                                                     <  1:239895/236‑1 (MQ=255)
                                                                                                  aTCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCa                                                                                                                         <  1:723446/235‑1 (MQ=255)
                                                                                                             aaGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACt                                                                                                             <  1:23027/236‑1 (MQ=255)
                                                                                                                          tCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTgg                                                                                                           <  1:133452/225‑1 (MQ=255)
                                                                                                                          tCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCg                                                                                                >  1:581446/1‑236 (MQ=255)
                                                                                                                                    tgtTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGTGGTCC‑TTCttt                                                                                     >  1:263087/1‑236 (MQ=255)
                                                                                                                                    tgtTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCggactatactt                                                                                      >  2:720792/1‑227 (MQ=255)
                                                                                                                                              ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGCTACTTTTTTGCAATGGGGTTGACTGGCAATATTGGCGGGTG‑TTCTTTACgcgtaagt                                                                           >  2:336400/1‑229 (MQ=255)
                                                                                                                                              ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCACTGGGGTGGACTGGCAATATTGGCGGGGA‑TTCctttttgtcactg                                                                           >  2:62214/1‑226 (MQ=255)
                                                                                                                                              ggCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCC‑TTCCTTACGGtgaatt                                                                           >  1:718685/1‑231 (MQ=255)
                                                                                                                                                ccggACCAGAAATAATCCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGa                                                                         <  2:199676/233‑1 (MQ=255)
                                                                                                                                                   gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATa                                                                                                                                   <  2:738157/176‑1 (MQ=255)
                                                                                                                                                   gACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATa                                                                                                                                   >  1:738157/1‑176 (MQ=255)
                                                                                                                                                               tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCACTGGGGTGGACTGGCAATATTGGCGGTCA‑TTTTTTTCTGGAAATTGGGGTCTGTTGGCTggg                                                          >  2:274893/1‑236 (MQ=255)
                                                                                                                                                               tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTTCGGTCACTTGAGTCCTGtcagcttgg                                                          >  2:223786/1‑228 (MQ=255)
                                                                                                                                                                gCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  2:566910/236‑1 (MQ=255)
                                                                                                                                                                gCGGTATTACTGCCCGCAGAGCAGTTATTACGGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  1:431502/236‑1 (MQ=255)
                                                                                                                                                                   gTATTACTGCCCGCAGTGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCTATAAGGGCGTTCTTACTGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAgg                                                      <  1:720792/236‑1 (MQ=255)
                                                                                                                                                                       tACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                         <  1:79186/229‑1 (MQ=255)
                                                                                                                                                                          tGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGt                                               <  1:11387/236‑1 (MQ=255)
                                                                                                                                                                              tgCAGAGCAGTGAGGACGGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTGACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATCCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtgg                                           <  2:446889/235‑1 (MQ=255)
                                                                                                                                                                              cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTTA‑TTCTTTTCGGTAAATTGAGTCCCGTAGGCTTGGCTGGGATggtgctcg                                           >  2:281339/1‑229 (MQ=255)
                                                                                                                                                                              cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCT‑TTCTTTTCGGTTACTTTAGTCCTGTTGGCTgggatggggttggt                                               >  2:79186/1‑221 (MQ=255)
                                                                                                                                                                              cGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTAGGGTAATTAGGGTCCTTTGGACGGGGCGGGactaggctag                                            >  2:492919/1‑227 (MQ=255)
                                                                                                                                                                                cAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggtg                                         <  2:156853/236‑1 (MQ=255)
                                                                                                                                                                                 agagCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggtgg                                        <  1:588276/236‑1 (MQ=255)
                                                                                                                                                                                     cAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa                                    <  1:492919/236‑1 (MQ=255)
                                                                                                                                                                                       gtgtgtaCTGGAAGCTTTATTTCGTATAGGCGATAAGTGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGat                                   <  2:718685/230‑1 (MQ=255)
                                                                                                                                                                                          attaCTGGAAGCGTTATGTGGTAAAGGCGTTAAGGGCGTTCTTACGGGTAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATg                                <  2:654380/235‑1 (MQ=255)
                                                                                                                                                                                             aCTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTgg                                                                                                                >  1:666812/1‑153 (MQ=255)
                                                                                                                                                                                             aCTGGAAGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTgg                                                                                                                <  2:666812/153‑1 (MQ=255)
                                                                                                                                                                                                  aatcGTAATTTCGTAAAGGTGATAAGGTCGTTCTAACGAGAAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  2:114284/233‑1 (MQ=255)
                                                                                                                                                                                                  aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                                                                                                                                    <  2:139785/96‑1 (MQ=255)
                                                                                                                                                                                                  aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGc                                                                                                                                                                    >  1:139785/1‑96 (MQ=255)
                                                                                                                                                                                                  aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACTGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGTGGTATGTttatta                         >  1:363402/1‑234 (MQ=255)
                                                                                                                                                                                                  aaGCGTTATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  1:274893/236‑1 (MQ=255)
                                                                                                                                                                                                  aaGCGTTAGTGCGTAAAGGTGAATAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                       <  2:49605/236‑1 (MQ=255)
                                                                                                                                                                                                        tATTTCGTAAAGGCGATAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCt                                                                    <  1:547990/185‑1 (MQ=255)
                                                                                                                                                                                                                       aTAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTTAGTTCTGTAGGCTGGGGCGGGATGAGTCTGGTTGGCGGTATGGTTATTATGGTCTGTTGGGTGATTTCtttt  >  1:765896/1‑236 (MQ=255)
                                                                                                                                                                                                                       aTAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGGTTATTCCtttt  >  1:442996/1‑236 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      
CGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTTTTGTGCCGATATCGTAAATCTGATTGTGAAAGGAAAAATTCATCTGGAAGATCAGTCTGACAAGAACCAGCAAATCCTGATTCGTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCA‑TTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTT  >  NC_000913/1325612‑1326062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A