Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,398,075 A→C I697M (ATT→ATG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,398,0750AC98.6% 256.8 / ‑6.2 73I697M (ATT→ATG) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (39/33);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTC  >  NC_000913/2397847‑2398296
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
gccCACTTCGTCCTGGAATTTGTGCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGa                                                                                                                                                                                                                        <  1:333001/236‑1 (MQ=255)
     cTTCGTCCTGGAATTGGTTCCAGGCCGTGGGGGAGTTCCAGCCCGGCCCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTc                                                                                                                                                                                                                   <  2:59469/236‑1 (MQ=255)
          tCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATc                                                                                                                                                                                                              <  1:599670/236‑1 (MQ=255)
              ggAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCgg                                                                                                                                                                                                           <  1:96442/235‑1 (MQ=255)
               gAATTTGTACCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGgcg                                                                                                                                                                                                         <  2:192822/236‑1 (MQ=255)
                   ttGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCgcag                                                                                                                                                                                                      <  1:304126/235‑1 (MQ=255)
                    tGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCgcagca                                                                                                                                                                                                    <  1:192286/236‑1 (MQ=255)
                    tGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCgcagca                                                                                                                                                                                                    >  1:93316/1‑236 (MQ=255)
                                                cGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         >  1:135295/1‑235 (MQ=255)
                                                  gcgcCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         <  1:508123/233‑1 (MQ=255)
                                                        aGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCcaca                                                                                                                                                                >  1:510987/1‑236 (MQ=255)
                                                                    cTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATc                                                                                                                                                    >  1:5824/1‑236 (MQ=255)
                                                                         gAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGGCCGGTTCACGGGCCAGCTTCTGCCCACGCGTACGGAATGTCGCATCCGGCGCAGCACCCTTGCTACCGTCACGATCCGGGCGTTTCGCCACAAACGCGTCAATcaccag                                                                                                                                               >  2:280500/1‑233 (MQ=255)
                                                                                 cgcAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCt                                                                                                                                       <  1:106231/236‑1 (MQ=255)
                                                                                 cgcAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCACACGGAATGTCGCATCCGCCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGGCAATAACATGGTCgcgat                                                                                                                                       >  2:308706/1‑232 (MQ=255)
                                                                                  gcAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATcaca                                                                                                                                                 <  1:295005/225‑1 (MQ=255)
                                                                                         gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTGCGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTg                                                                                                                                      >  1:465771/1‑229 (MQ=255)
                                                                                         gCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCCCGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCCCACAAGCGTCAATCACACGGTCGa                                                                                                                                          >  2:295005/1‑225 (MQ=255)
                                                                                           tGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtc                                                                                                                             <  1:21508/236‑1 (MQ=255)
                                                                                           tGGTTCTTACCTGCCACGGAGAAGGTGAACAGGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtc                                                                                                                             <  2:510987/236‑1 (MQ=255)
                                                                                             gttgttACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCgtccagtcc                                                                                                                            >  2:217583/1‑235 (MQ=255)
                                                                                                       tCCATCGAGAAGGTGAACGTGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCt                                                                                                                 <  2:135295/236‑1 (MQ=255)
                                                                                                       tCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGc                                                                                                                  >  1:486701/1‑235 (MQ=255)
                                                                                                        ccATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         >  1:185688/1‑179 (MQ=255)
                                                                                                        ccATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         <  2:185688/179‑1 (MQ=255)
                                                                                                              agaAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCa                                                                                                                      <  1:41939/224‑1 (MQ=255)
                                                                                                              agaAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCa                                                                                                                      >  2:41939/1‑224 (MQ=255)
                                                                                                                  ggTGAACATGGTGTCAATTTCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTg                                                                                                       <  1:650903/235‑1 (MQ=255)
                                                                                                                  ggTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTcagcag                                                                                                           >  1:460320/1‑231 (MQ=255)
                                                                                                                   gTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGc                                                                                                      >  1:36816/1‑235 (MQ=255)
                                                                                                                      aaCATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCtt                                                                                                                                                                                                >  2:363892/1‑142 (MQ=255)
                                                                                                                      aaCATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCtt                                                                                                                                                                                                <  1:363892/142‑1 (MQ=255)
                                                                                                                             tgtCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCCCAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACTGCTCCGCACGAGGCGGTGAAGCGAg                                                                                           >  2:30192/1‑236 (MQ=255)
                                                                                                                                       cTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAAc                                                                                     <  1:246740/232‑1 (MQ=255)
                                                                                                                                       cTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAAc                                                                                     >  2:246740/1‑232 (MQ=255)
                                                                                                                                       cTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAgc                                                                                 >  1:427027/1‑236 (MQ=255)
                                                                                                                                         gCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCCCAGCAGGGTGCTGTGCAGCGAGTGTAACCa                                                                                   >  1:284544/1‑232 (MQ=255)
                                                                                                                                               gACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCgcagc                                                                                                                                                                                                     <  1:226680/112‑1 (MQ=255)
                                                                                                                                               gACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCgcagc                                                                                                                                                                                                     >  2:226680/1‑112 (MQ=255)
                                                                                                                                                           gAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATcaca                                                                                                                                                 <  2:291155/152‑1 (MQ=255)
                                                                                                                                                           gAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATcaca                                                                                                                                                 >  1:291155/1‑152 (MQ=255)
                                                                                                                                                                cTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGaacgt                                                           >  1:228303/1‑229 (MQ=255)
                                                                                                                                                                cTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGt                                                           >  1:617540/1‑233 (MQ=255)
                                                                                                                                                                cTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGt                                                           >  1:344275/1‑233 (MQ=255)
                                                                                                                                                                   atatTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGt                                                           >  1:279035/1‑230 (MQ=255)
                                                                                                                                                                    ttgtGGCGCGCGTGGCGGTACGACCTCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAAGGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTg                                                    <  2:5824/233‑1 (MQ=255)
                                                                                                                                                                    tatTGGCGCGAATGCCGTTACGACCGCTGTAACGGTGCGGTTAACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTGGATACCTGACAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTg                                                    <  2:93316/236‑1 (MQ=255)
                                                                                                                                                                        tgcccgcATGTCGGTACGCCCGGTGTACCGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCg                                                 <  1:682669/231‑1 (MQ=255)
                                                                                                                                                                         gcgcgtATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGta                                               <  2:617540/236‑1 (MQ=255)
                                                                                                                                                                         gcgcgcATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGta                                               <  1:130184/236‑1 (MQ=255)
                                                                                                                                                                         gcgcgcATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGta                                               <  1:217583/236‑1 (MQ=255)
                                                                                                                                                                         gcgcgcATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCGTTCCAGCATGACAGTTTTGCTGTCGta                                               >  2:219487/1‑236 (MQ=255)
                                                                                                                                                                         gagcgcTTGGCGGTACGACCGCTGTACCGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATTCGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGta                                               <  2:228303/234‑1 (MQ=255)
                                                                                                                                                                           gcgcATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGGAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGtaa                                              >  1:277477/1‑235 (MQ=255)
                                                                                                                                                                                tGGGGGTACGCCCGATGAACCGGTGCGTTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCa                                        <  2:36816/236‑1 (MQ=255)
                                                                                                                                                                                 ggCGGTCCGACGGCTTTACGGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAAGGTCGCATCCGGCGCAGCATCTTTGATCCCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCa                                        <  2:589866/235‑1 (MQ=255)
                                                                                                                                                                                    gttccGACCGCTGTACCGGTGCGGTTCACGGGCCAGTTTCGCCCCACCCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAg                                       <  2:284544/229‑1 (MQ=255)
                                                                                                                                                                                      tACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGGAGTAAGCAGGATCg                                  >  2:711446/1‑236 (MQ=255)
                                                                                                                                                                                           ccGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         <  1:219349/96‑1 (MQ=255)
                                                                                                                                                                                           ccGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGAtt                                                                                                                                                                         >  2:219349/1‑96 (MQ=255)
                                                                                                                                                                                              cTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTg                          >  1:455693/1‑236 (MQ=255)
                                                                                                                                                                                              cTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGAAAGGTTTGCTGTCGTCATAAGCAGGACCGTAAAACTg                          >  2:289208/1‑236 (MQ=255)
                                                                                                                                                                                                         cGGTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGt                >  1:411913/1‑235 (MQ=255)
                                                                                                                                                                                                           gTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTg                                                    >  2:333560/1‑197 (MQ=255)
                                                                                                                                                                                                           gTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTg                                                    <  1:333560/197‑1 (MQ=255)
                                                                                                                                                                                                           gTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGtttg             >  1:545502/1‑234 (MQ=255)
                                                                                                                                                                                                           gTTCACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTgg             >  1:263679/1‑236 (MQ=255)
                                                                                                                                                                                                              cACGGGCCAGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGgc           >  2:377234/1‑235 (MQ=255)
                                                                                                                                                                                                                   gcaaGTTGCTGCCCCGGAAGTCGGAATGGCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGcc     <  2:287984/233‑1 (MQ=255)
                                                                                                                                                                                                                      aGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTc  >  1:503185/1‑236 (MQ=255)
                                                                                                                                                                                                                      aGTTTCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTc  >  1:679851/1‑236 (MQ=255)
                                                                                                                                                                                                                        tttCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCAt                                                                 <  2:563249/171‑1 (MQ=255)
                                                                                                                                                                                                                        tttCTGCCCACGCATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCAt                                                                 >  1:563249/1‑171 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
GCCCACTTCGTCCTGGAATTTGTTCCACGCCTGCGGGGAGTTCCAGCCCGGCGCCCAGGCAAACGGCACTTGCGAACGGTGCGCAGTCGGCTGGTTGTTACCTTCCATCGAGAAGGTGAACATGGTGTCAATATCCTGCGGCTGACGCGGCTCATGAACGCTGATATTGGCGCGCATGGCGGTACGACCGCTGTAACGGTGCGGTTCACGGGCCAGTTTCTGCCCACGAATACGGAATGTCGCATCCGGCGCAGCATCTTTGATACCTGCCAGTTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTC  >  NC_000913/2397847‑2398296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A