Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I51 R1 223 69.9 1534784 95.1% 1459579 214.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,547,053 T→G G127G (GGT→GGG)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,547,0530TG95.3% 269.7 / ‑5.1 85G127G (GGT→GGG) murPN‑acetylmuramic acid permease, EIIBC component, PTS system
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base G (39/42);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTC  >  NC_000913/2546828‑2547275
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                              
cGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTgccg                                                                                                                                                                                                                      >  1:430363/1‑236 (MQ=255)
cGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGCTAATTgccg                                                                                                                                                                                                                      >  1:233589/1‑236 (MQ=255)
cGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGGCAGGTAAAAGCCAAACAAACCTCCGGCGTGCAAAAATTTCTCGCCCAATCAGCCACCACCTTACAGCCGCTGATCCCCGGGTTAATTgccg                                                                                                                                                                                                                      >  1:610969/1‑236 (MQ=255)
   gAAGGGCTTCATTTTGACCAGCGACCAGGGGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGTCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTgccgc                                                                                                                                                                                                                     <  1:321517/234‑1 (MQ=255)
   gAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTgccgc                                                                                                                                                                                                                     <  1:526666/234‑1 (MQ=255)
              ttttGGCCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctgctgct                                                                                                                                                                                                         <  1:767185/235‑1 (MQ=255)
               tttGGCCATGGACCAGGTGCAGGTCTTTATTGGTCCTGGTAAACCGCATCGTGCCGCAAAAGCG‑ATTAGCGAGCTGATGGGAGAAGCGCCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctg                                                                                                                                                                                                              <  2:228318/229‑1 (MQ=255)
                        cGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctgctgct                                                                                                                                                                                                         <  1:611472/225‑1 (MQ=255)
                        cGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctgctgct                                                                                                                                                                                                         >  2:611472/1‑225 (MQ=255)
                            cAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCg                                                                                                                                                                                               <  1:383745/231‑1 (MQ=255)
                              ggTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTa                                                                                                                                                                                         <  1:81165/235‑1 (MQ=255)
                                  cAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctg                                                                                                                                                                                                              <  2:111970/210‑1 (MQ=255)
                                  cAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctg                                                                                                                                                                                                              >  1:111970/1‑210 (MQ=255)
                                   tagcagTTTTGGGACCTGGTAAAGGCCAGTGTGACAGAAAGCCG‑ATGAGGCAGCTGGTGGGAGAAGCCCCGGTACA‑GGATGCCGCAGAAACCGCCGCCCAGAA‑CAAACGTAAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGtt                                                                                                                                                                                          <  2:736220/224‑1 (MQ=255)
                                    ggTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCa                                                                                                                                                                                  <  1:107878/236‑1 (MQ=255)
                                         tttttGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGGTGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGt                                                                                                                                                                              >  1:425400/1‑235 (MQ=255)
                                         tgtttGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAACCGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCa                                                                                                                                                                                  <  2:261902/229‑1 (MQ=255)
                                                gCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctg                                                                                                                                                                                                              >  2:553468/1‑196 (MQ=255)
                                                gCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTctg                                                                                                                                                                                                              <  1:553468/196‑1 (MQ=255)
                                                           cgcATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCAACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCCCGCTCCGGAAGa                                                                                                                                                            >  2:670359/1‑235 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGCTGGCGACGTTCATTGCCACGGTGATGCACGTTCCGGcccatgttc                                                                                                                                                       >  2:119568/1‑228 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCa                                                                                                                                                                        >  2:688349/1‑219 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCa                                                                                                                                                                        <  1:688349/219‑1 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCCGATGCTc                                                                                                                                                       >  1:739397/1‑236 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGc                                                                                                                                                         <  1:697504/234‑1 (MQ=255)
                                                               tCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTc                                                                                                                                                       >  1:626635/1‑236 (MQ=255)
                                                                cGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGT‑AGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAg                                                                                                                                                     >  1:468627/1‑236 (MQ=255)
                                                                 gggCCGCAAGAGCG‑ATGGGCTAGCTGCTGGACAAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAg                                                                                                                                                     <  2:430363/234‑1 (MQ=255)
                                                                  ttccGCAAAGGCG‑ATGACCGAGCCGGTGGGAGAAGCACCGGTACA‑GGCTGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCACCAATTTCTCGCCAAATTCGCCCCCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAgg                                                                                                                                                    <  2:19474/234‑1 (MQ=255)
                                                                      gCAAAAGCG‑ATGGGCGCGCGTGTGGGAGAACCTCCGATACG‑GGATGCCGCAAAAATCGCCGCCAAGAA‑CAAACG‑AAGTTAAAAGCCAAACAACCCCCCGGCTTGCAACAATTTCCCGCCAATTTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAaca                                                                                                                                               <  2:468627/236‑1 (MQ=255)
                                                                      gCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGaa                                                                                                                                                 <  1:500251/235‑1 (MQ=255)
                                                                       gaaaaGCGAATGAGCAGGCCGGCGGAAGAATCACCGG‑ACAGGGATGCCGCAAAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCGCCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGaa                                                                                                                                                 <  2:739397/234‑1 (MQ=255)
                                                                                  gagcgagcTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATg                                                                                                                                     >  1:224227/1‑236 (MQ=255)
                                                                                         ctgctgGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGaa                                                                                                                              <  1:119568/236‑1 (MQ=255)
                                                                                            ctgGGAAAAGCCCCGGTACA‑GGATGCCGCAGAAATCGCCGCCAAGAA‑CAAACGTCAGTTAAAAGCCAAACAACCCCCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAAtt                                                                                                                            <  2:224227/235‑1 (MQ=255)
                                                                                             tcagagaAGCACCGGTACA‑GGCTGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCCCCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGaa                                                                                                                              <  2:555108/229‑1 (MQ=255)
                                                                                                      cACCGGTACA‑GGGTGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGACGgtg                                                                                                                 >  1:610574/1‑236 (MQ=255)
                                                                                                          ggTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCa                                                                                                             >  2:134735/1‑236 (MQ=255)
                                                                                                                agcatcccGCATAAATCGCCGCCAAGAA‑CAAGCGTCAGTTAAAAGCCAAACAACCCTCCGGCGTGCCACAATTTATCGCCAAGTCCGCCACCATCTTCACGCTGCTGATCCCCTGGTTTATGGCCGCCGGTCTGATGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAg                                                                                                            <  2:434037/226‑1 (MQ=255)
                                                                                                                    tGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGACGTTTATGCAGGAGTTTAGCAAAGGtg                                                                                                    >  2:330424/1‑235 (MQ=255)
                                                                                                                    tGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCCAAGGtt                                                                                                    >  2:618889/1‑236 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTAcc                                                                                                                                          >  1:100835/1‑195 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGaa                                                                                                                              >  1:563869/1‑207 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGaa                                                                                                                              <  2:563869/207‑1 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGtt                                                                                                               >  1:283162/1‑222 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGtt                                                                                                               <  2:283162/222‑1 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCACAGGctt                                                                                                   >  2:434850/1‑234 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTg                                                                                                  >  1:453426/1‑235 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGGTTg                                                                                                  >  1:379104/1‑235 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGCTTGt                                                                                                 >  1:507125/1‑236 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGAGGCTCAGGGAACACTACCCGATGCGCCGAATTTTATGAAGGTGTTCAGCAACGGATTg                                                                                                  >  2:724514/1‑235 (MQ=255)
                                                                                                                       cGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTACGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTg                                                                                                  >  2:454765/1‑235 (MQ=255)
                                                                                                                           agaaccccccgccAGAATCAACCGTCAGTTAAAAGCCAAACAACCCCCCGTCGTCCAACAATTTCTCCCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCa                                                                                              <  2:94725/225‑1 (MQ=255)
                                                                                                                              aTCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGACTTTTATGAAGGGGTTCAGCAAAGGCTGGTTCAc                                                                                             >  2:568954/1‑233 (MQ=255)
                                                                                                                                cgccgcCCAGAA‑AAAGCGTCAGTTAAAAGCCAAACAACCCTCCGGCTTGCAACAATTTCTCGCCAATTTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTcc                                                                                        <  1:670359/236‑1 (MQ=255)
                                                                                                                                 gcccccCAGAA‑CAATCGCACGTTAACAGCCAACCAGCCCTCCGGCGTGCAACAATTACTCGACAAATTCGCCCACATCTTCACGCCGCTGATCCCCTGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCt                                                                                       <  2:595139/236‑1 (MQ=255)
                                                                                                                                      ccAGAA‑CAAACGTCAGTTAAAAGCCAAACAACCCTCCGGCCTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGa                                                                                  <  1:316150/236‑1 (MQ=255)
                                                                                                                                             cAAACGTCAGTTAAAAGCCAACCAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCtgg                                                                            <  1:107282/236‑1 (MQ=255)
                                                                                                                                              aaaCGTAAGTTAAACCCCAAACAAACCCCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGtt                                                                                                               <  2:670753/200‑1 (MQ=255)
                                                                                                                                               aaCGTCAGTAAAAAGCCAAACAGACCTCCGGTATGCACCCATTTCACGCCAAATTCGCCACGATCTTAACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCtggtg                                                                          <  2:592718/236‑1 (MQ=255)
                                                                                                                                                aCGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCtggtgg                                                                         >  2:338041/1‑236 (MQ=255)
                                                                                                                                                     aGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCaaa                                                                                                        >  1:670753/1‑200 (MQ=255)
                                                                                                                                                           agcccAAAAAACCCCCCGGCGGGCAACATTTTCCCGCCAACTTCGCCCCCATCTGCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTggg                                                                        <  2:453426/223‑1 (MQ=255)
                                                                                                                                                           aaGCAAAACCAGCCTCCGGCCTGCAACAATTCCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATGGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAAcg                                                                <  2:507125/234‑1 (MQ=255)
                                                                                                                                                            aGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAAcgccg                                                             >  1:504325/1‑236 (MQ=255)
                                                                                                                                                                 aacaaacCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAg                                                                                                                    <  2:450640/176‑1 (MQ=255)
                                                                                                                                                                 aacaaacCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAg                                                                                                                    >  1:450640/1‑176 (MQ=255)
                                                                                                                                                                            ggCGTGCAACATTTCCTCGCCAAATTCGCCACCTTCTCCACGCCGCTGACCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGCATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTggcgg                                             <  2:379104/236‑1 (MQ=255)
                                                                                                                                                                              tgTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCCCGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCCCTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCa                                           <  1:491036/235‑1 (MQ=255)
                                                                                                                                                                              ggTGCATCGATTCCTCGCCAAATTCACACCCTTCTTCAGGCCGGTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCa                                           <  2:474886/235‑1 (MQ=255)
                                                                                                                                                                              cGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCa                                           <  1:134735/236‑1 (MQ=255)
                                                                                                                                                                                tGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGTCGGAACg                                         >  2:736436/1‑236 (MQ=255)
                                                                                                                                                                                  caacaaTTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGTTGGGCTATAACGCCGCCCTGGCATTTgtc                                               >  2:490910/1‑226 (MQ=255)
                                                                                                                                                                                            tccccAAATTCGCCCCCATTTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCCGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGgc                             <  1:571352/233‑1 (MQ=255)
                                                                                                                                                                                            ccgcaaactgcGCCATCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTaaa                                 <  2:233589/222‑1 (MQ=255)
                                                                                                                                                                                             cGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGAATTTGGCGGCACGGGCGTAAATGgcg                            >  1:154356/1‑236 (MQ=255)
                                                                                                                                                                                                                     gccgcTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGc    >  2:136490/1‑236 (MQ=255)
                                                                                                                                                                                                                       cgttGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTc  <  1:330424/233‑1 (MQ=255)
                                                                                                                                                                                                                       cgcTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTTCCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTc  <  1:268210/236‑1 (MQ=255)
                                                                                                                                                                                                                         cTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGa                                                                                                                                                                                              <  1:64702/46‑1 (MQ=255)
                                                                                                                                                                                                                         cTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGa                                                                                                                                                                                              >  2:64702/1‑46 (MQ=255)
                                                                                                                                                                                                                         cTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAg                                                        >  2:277907/1‑180 (MQ=255)
                                                                                                                                                                                                                         cTGATCCCCGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAg                                                        <  1:277907/180‑1 (MQ=255)
                                                                                                                                                                                                                                 cGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCa                                           >  1:744488/1‑185 (MQ=255)
                                                                                                                                                                                                                                 cGGGTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCa                                           <  2:744488/185‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                              
CGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCG‑ATGAGCGAGCTGCTGGGAGAAGCACCGGTACA‑GGATGCCGCAGAAATCGCCGCCCAGAA‑CAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGTTAATTGCCACGGTGATGCACGTTCCGGCAGATGCTCAGGGAACACTACCCGATGCGCTGAATTTTATGAAGGTGTTCAGCAAAGGTTTGTTCACTTTCCTGGTGATTCTGGTGGGCTATAACGCCGCCCAGGCATTTGGCGGCACGGGCGTAAATGGCGCAATTATCGCCGCGCTCTTTTTGCTC  >  NC_000913/2546828‑2547275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A