Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F422 I51 R1
|
223 |
69.9 |
1534784 |
95.1% |
1459579 |
214.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,624,901 |
T→G |
Y620D (TAT→GAT) |
ppk → |
polyphosphate kinase, component of RNA degradosome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,624,901 | 0 | T | G | 100.0%
| 133.9
/ NA
| 40 | Y620D (TAT→GAT) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/23); total (17/23) |
GGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACA > NC_000913/2624678‑2625125
|
ggTTGATCGTCTGTATGCGTCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGaa < 1:349640/235‑1 (MQ=255)
tCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGGATTTAAGTGGCg > 1:305092/1‑235 (MQ=255)
tGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCAGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGAc > 1:577201/1‑232 (MQ=255)
ccTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGa > 2:41691/1‑235 (MQ=255)
ccAGGTCCGGCTTACCGGTTAATCGGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATcc < 2:85129/235‑1 (MQ=255)
ccAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATcc < 1:135374/235‑1 (MQ=255)
ggCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTg < 1:127233/234‑1 (MQ=255)
gCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAag > 2:551116/1‑236 (MQ=255)
ctgctgGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGa < 2:312184/236‑1 (MQ=255)
ggTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTTATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTTAAGCAGCGGTGACTGTAcatc > 2:223153/1‑235 (MQ=255)
ggTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGAcatta < 1:45242/236‑3 (MQ=255)
aaTCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaa < 1:223153/236‑1 (MQ=255)
aTCGGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCaaa < 2:375671/236‑1 (MQ=255)
tGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAgaa > 1:629125/1‑234 (MQ=255)
gAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGGCATCATCGACATAATGTTCAGCGATACGGGCAAAGCACg > 2:662163/1‑236 (MQ=255)
ggCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGtt > 1:261348/1‑235 (MQ=255)
tCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCa > 1:248091/1‑236 (MQ=255)
tGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTC‑AAGCACGTTATATCGATAAAGAACTCAGTaa < 1:551116/236‑1 (MQ=255)
aCCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGGCATCATCTACATATTGTTCAGCGATACGGTCAAAGCAAGTTATATAGATCAAGAACCCACTAATCGTTACGTTccccac > 2:172967/1‑234 (MQ=255)
cGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCgg > 1:28420/1‑236 (MQ=255)
ttACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAagca > 2:1469/1‑141 (MQ=255)
ttACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAagca < 1:1469/141‑1 (MQ=255)
gACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGCTAAAGAACTCAGTAATCGCTACGTTCACCCACGCAATCGCCGCAAAGt > 1:605865/1‑236 (MQ=255)
atttATGTTTTTGAAATTGGCGGTTATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACggg < 2:295944/234‑1 (MQ=255)
tttaGAAAAGGGCAGTGATAAGAAGGTCTACCTTTCTTCCGCCGACTGGATGACGTGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTgg < 2:577201/236‑1 (MQ=255)
ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAg < 1:496795/177‑1 (MQ=255)
ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAg > 2:496795/1‑177 (MQ=255)
ttGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATTGCCGCAAAGTACGGGGGCAGTTgtgg > 2:290362/1‑233 (MQ=255)
ataTGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGAAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGAttt < 2:713965/234‑1 (MQ=255)
aaaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCaa < 1:238947/236‑1 (MQ=255)
aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCaa < 1:617539/235‑1 (MQ=255)
aaaaGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCaa < 1:662163/235‑1 (MQ=255)
aGGTCTACCTTGCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCa < 1:81408/236‑1 (MQ=255)
tACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGa > 2:565603/1‑236 (MQ=255)
ccTTTCTTCCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGa < 2:248091/234‑1 (MQ=255)
ttcttcCGCCGACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaacaac < 1:565603/236‑1 (MQ=255)
ttctccCGTCAACTGGATGACGCGCAATTGTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGaacaac < 2:261348/236‑1 (MQ=255)
cggcagtcggATGACCGGCAATATTGATGATCGGATTGAAGTGGCGACGCCGCTGCTCGTTCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAAt < 2:305092/228‑1 (MQ=255)
gccgACTGGATGACGCGCAATATTGATGATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAAt > 1:110329/1‑235 (MQ=255)
cAATATTGAGGATCGTATGGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATaca < 1:290362/235‑1 (MQ=255)
|
GGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTACCTTTCTTCCGCCGACTGGATGACGCGCAATATTGATTATCGTATTGAAGTGGCGACGCCGCTGCTCGATCCGCGCCTGAAGCAGCGGGTACTGGACATCATCGACATATTGTTCAGCGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACA > NC_000913/2624678‑2625125
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
