Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F433 I3 R1 16 161.1 8199666 93.6% 7674887 99.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,360,668 C→T G81S (GGC→AGC)  puuA ← glutamate‑‑putrescine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,360,6680CT97.2% 443.6 / ‑2.4 144G81S (GGC→AGC) puuAglutamate‑‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base T (63/77);  total (67/77)
Fisher's exact test for biased strand distribution p-value = 4.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

ATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCAT  >  NC_000913/1360570‑1360766
                                                                                                  |                                                                                                  
aTCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGcc                                                                                                    <  1:2698097/99‑1 (MQ=255)
    gcggcAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGa                                                                                                >  2:2237404/1‑99 (MQ=255)
      ggcAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTCCGTTGCTCAGAat                                                                                              >  1:1925344/1‑99 (MQ=255)
      ggcAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAat                                                                                              >  1:3374767/1‑99 (MQ=255)
      ggcAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAat                                                                                              >  1:1765826/1‑99 (MQ=255)
        cAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAatat                                                                                            <  1:2052976/99‑1 (MQ=255)
           aaGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCAGTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTTAGAATATCCa                                                                                         <  1:3628087/99‑1 (MQ=255)
            aGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCAt                                                                                        <  1:2775928/99‑1 (MQ=255)
            aGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCAt                                                                                        <  1:2257625/99‑1 (MQ=255)
             ggAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCAtt                                                                                       <  1:3485558/99‑1 (MQ=255)
              gAGTTAAGGAACCGATAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTg                                                                                      <  1:1055456/99‑1 (MQ=255)
              gAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTg                                                                                      <  1:1437678/99‑1 (MQ=255)
               aGTTAACAAACCGAGAACAGGACCACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGCCCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGc                                                                                     <  1:3938034/99‑1 (MQ=255)
                gTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCa                                                                                    >  2:1909685/1‑99 (MQ=255)
                gTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTCCa                                                                                    >  2:3212155/1‑99 (MQ=255)
                 ttAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCaa                                                                                   >  2:4001903/1‑99 (MQ=255)
                 ttAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCaa                                                                                   >  1:1466443/1‑99 (MQ=255)
                   aaGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAAt                                                                                 >  2:2392427/1‑99 (MQ=255)
                     ggAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATAc                                                                               >  1:1468610/1‑99 (MQ=255)
                     ggAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATAc                                                                               <  1:1097689/99‑1 (MQ=255)
                      gAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATAcc                                                                              >  1:1328002/1‑99 (MQ=255)
                             gaACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGAGCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCg                                                                       <  2:135546/99‑1 (MQ=255)
                             gaACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCg                                                                       <  2:2995053/99‑1 (MQ=255)
                              aaCAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTTCTCAGAATATCCATTGCAAATACCGAGGCCgg                                                                      >  2:1014342/1‑99 (MQ=255)
                               aCAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCggg                                                                     <  2:1206249/99‑1 (MQ=255)
                                cAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGa                                                                    <  1:1251550/99‑1 (MQ=255)
                                cAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGa                                                                    >  2:3683830/1‑99 (MQ=255)
                                 aGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGaa                                                                   >  1:3203279/1‑99 (MQ=255)
                                  ggAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGaaa                                                                  <  2:2765288/99‑1 (MQ=255)
                                  ggAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATCGCAAATACCGAGGCCGGGaaa                                                                  <  2:1469396/99‑1 (MQ=255)
                                   gAACACAGGTACGATCCGGCTCGCCCCTTTCCTGACCCAGACCTGATTCTTCTACTACGTTGCTCAGAATATCAATTGCAAATACCGCGGCCGGGACAt                                                                 >  1:1020415/1‑99 (MQ=255)
                                   gAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAAt                                                                 >  1:2557766/1‑99 (MQ=255)
                                   gAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAAt                                                                 >  2:2365250/1‑99 (MQ=255)
                                   gAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAAt                                                                 >  1:722666/1‑99 (MQ=255)
                                    aaCACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATa                                                                >  2:2125907/1‑99 (MQ=255)
                                    aaCACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATa                                                                >  1:796684/1‑99 (MQ=255)
                                      cacaGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAac                                                              <  1:4078648/99‑1 (MQ=255)
                                       acaGGTACGATCCGGCTCGCCCATTTCCTGACCCGGACCTGCCTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAact                                                             >  2:101916/1‑98 (MQ=255)
                                        caGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAacac                                                            <  2:2865128/99‑1 (MQ=255)
                                        caGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAacac                                                            <  1:2371894/99‑1 (MQ=255)
                                           gTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCt                                                         <  2:3860064/99‑1 (MQ=255)
                                           gTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCCAGGCCGGGGAATAACACCCt                                                         >  1:4130162/1‑99 (MQ=255)
                                           gTACGATCCGGCTCGCCCATTTCCTGACCCAGACCGGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGAAAATAACACCCt                                                         <  2:4001866/99‑1 (MQ=255)
                                            tACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCtt                                                        >  1:2026643/1‑99 (MQ=255)
                                             aCGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCttt                                                       >  1:1773770/1‑99 (MQ=255)
                                                aTCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTtctc                                                    >  2:1954873/1‑99 (MQ=255)
                                                 tCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCg                                                   >  2:3496012/1‑99 (MQ=255)
                                                 tCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCg                                                   <  2:2713404/99‑1 (MQ=255)
                                                  ccGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGa                                                  <  1:823550/99‑1 (MQ=255)
                                                  ccGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGa                                                  <  1:3727530/99‑1 (MQ=255)
                                                  ccGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGa                                                  <  2:1176316/99‑1 (MQ=255)
                                                  ccGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGa                                                  <  1:3366986/99‑1 (MQ=255)
                                                  ccGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGa                                                  <  1:2270289/99‑1 (MQ=255)
                                                   cGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAg                                                 <  1:2544784/99‑1 (MQ=255)
                                                     gCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGct                                               >  1:579600/1‑99 (MQ=255)
                                                       tCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGcttc                                             <  1:3094541/99‑1 (MQ=255)
                                                         gCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGcttctt                                           <  2:1397337/99‑1 (MQ=255)
                                                         gCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGcttctt                                           >  2:1087880/1‑99 (MQ=255)
                                                          cccATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCttcttc                                          <  1:1662167/99‑1 (MQ=255)
                                                          cccATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCttcttc                                          <  1:2678646/99‑1 (MQ=255)
                                                           ccATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAACTAACACCCTTTCTCGAGCTTCTTCa                                         >  1:797633/1‑99 (MQ=255)
                                                           ccATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCa                                         >  1:725675/1‑99 (MQ=255)
                                                             aTTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAgg                                       <  2:3705731/99‑1 (MQ=255)
                                                             aTTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAgg                                       <  1:3307831/99‑1 (MQ=255)
                                                              tttCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGc                                      <  2:403980/99‑1 (MQ=255)
                                                              tttCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGc                                      <  1:3124522/99‑1 (MQ=255)
                                                                tCCTGACCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCTAGCTTCTTCAGGCtt                                    <  2:2836565/99‑1 (MQ=255)
                                                                     aCCCAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAAc                               <  2:827975/99‑1 (MQ=255)
                                                                      cccAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGNAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACa                              <  1:2405372/99‑1 (MQ=255)
                                                                      cccAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACa                              <  1:301763/99‑1 (MQ=255)
                                                                      cccAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACa                              <  2:3025887/99‑1 (MQ=255)
                                                                       ccAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAg                             >  2:3011448/1‑99 (MQ=255)
                                                                       ccAGACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACACTTTCTCGAGCTTCTTCAGGCTTGAAACAg                             >  2:2717416/1‑99 (MQ=255)
                                                                          gACCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGaa                          <  1:2102448/99‑1 (MQ=255)
                                                                           aCCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAAt                         <  1:1907233/99‑1 (MQ=255)
                                                                           aCCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAAt                         <  1:2399949/99‑1 (MQ=255)
                                                                           aCCTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAAt                         >  2:620558/1‑99 (MQ=255)
                                                                           aCCCGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGAGACATAACAACCTTTATCGAGCTTCTTCCGTCGTGAAACAGGAAt                         >  2:3430247/1‑99 (MQ=255)
                                                                            ccTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATa                        <  1:2804193/99‑1 (MQ=255)
                                                                             cTGCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATAc                       <  1:1505636/99‑1 (MQ=255)
                                                                               ggttctacTACTACGGCGCTGACAAGAACCATTGCAGATATCGAGGCCGCCAACTAACACCCTTTCTCGAGTTTTCACAGGCTTTAAACGCGAATTCGc                     <  2:748752/97‑1 (MQ=255)
                                                                               gCTTCTTCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCCTCTTCAGGCTTGAAACAGGAATACGc                     <  2:4036834/99‑1 (MQ=255)
                                                                                cttcttCTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCt                    >  2:105383/1‑99 (MQ=255)
                                                                                 ttcttcTACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCtt                   <  2:862006/99‑1 (MQ=255)
                                                                                   cttctACTACGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTc                 >  1:763527/1‑99 (MQ=255)
                                                                                      ctactaCGTTGCTCAGAATATCCACTGCAACTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCAc              <  2:1491126/99‑1 (MQ=255)
                                                                                       tactacGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACg             >  1:83907/1‑99 (MQ=255)
                                                                                          tacGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGCATACGCTTTCCACGGaa          >  2:1981502/1‑99 (MQ=255)
                                                                                          tacGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGaa          >  2:3144449/1‑99 (MQ=255)
                                                                                          tacGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGaa          <  1:1833404/99‑1 (MQ=255)
                                                                                          tacGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGaa          <  1:1142449/99‑1 (MQ=255)
                                                                                           acGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAg         <  2:2265399/99‑1 (MQ=255)
                                                                                            cGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGc        <  2:3117448/99‑1 (MQ=255)
                                                                                            cGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGc        <  1:4071477/99‑1 (MQ=255)
                                                                                            cGTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGc        <  2:842699/99‑1 (MQ=255)
                                                                                             gTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCa       >  1:3616506/1‑99 (MQ=255)
                                                                                             gTTGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCa       <  1:2698691/99‑1 (MQ=255)
                                                                                              ttGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCaa      <  1:2953398/99‑1 (MQ=255)
                                                                                              ttGCTCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACACTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCaa      <  2:4023824/99‑1 (MQ=255)
                                                                                                  ccAGAATTTCCATTGCAAATACCGAGGCTGGTAAATAGCAGCCTTGCTCGAGCTTCTTCCGGCTTGCAGCTGGAATACGCTGTCCACGGAAGCAACCAt  <  2:1515827/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
ATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCAT  >  NC_000913/1360570‑1360766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A