Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 533,167 C→T P53S (CCG→TCG)  allR → glyoxylate‑inducible transcriptional repressor of all and gcl operons

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913533,1670CT99.1% 768.6 / ‑4.9 222P53S (CCG→TCG) allRglyoxylate‑inducible transcriptional repressor of all and gcl operons
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (92/127);  total (93/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CGCACAGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGCCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAGGT  >  NC_000913/533070‑533262
                                                                                                 |                                                                                               
cgcACAGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCg                                                                                               <  2:5153242/100‑1 (MQ=255)
  cacaGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGct                                                                                             >  2:1238586/1‑100 (MQ=255)
  cacaGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTAGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATGTCTCTCAATCTGGCTTTGTCGct                                                                                             >  1:4852386/1‑100 (MQ=255)
   acaGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGctc                                                                                            <  1:1809502/100‑1 (MQ=255)
   acaGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGctc                                                                                            <  2:2688780/100‑1 (MQ=255)
       gCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCa                                                                                        <  2:2929425/100‑1 (MQ=255)
        cGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCAc                                                                                       <  1:3235873/100‑1 (MQ=255)
        cGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCAc                                                                                       <  2:3160227/100‑1 (MQ=255)
        cGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCAc                                                                                       >  2:2900153/1‑100 (MQ=255)
           tAGAGCGGGGAATTGCGATTTTGCCATATTTGGTAAAAAGTGGAGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGTATTTGTCGCTCTCCACGAc                                                                                    <  2:680353/100‑1 (MQ=255)
           tAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGAc                                                                                    <  1:4677272/100‑1 (MQ=255)
           tAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGAc                                                                                    <  1:2099647/100‑1 (MQ=255)
             gagCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGAccn                                                                                  <  1:4437891/100‑2 (MQ=255)
             gagCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCt                                                                                  <  1:1000983/100‑1 (MQ=255)
             gagCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCt                                                                                  <  1:3179634/100‑1 (MQ=255)
                cGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTc                                                                               <  1:3991801/100‑1 (MQ=255)
                  gggAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGc                                                                             <  1:867631/100‑1 (MQ=255)
                      aTTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGc                                                                         <  1:5341963/100‑1 (MQ=255)
                      aTTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGc                                                                         <  1:2262086/100‑1 (MQ=255)
                      aTTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGc                                                                         <  1:338930/100‑1 (MQ=255)
                       ttGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCt                                                                        <  1:1702179/100‑1 (MQ=255)
                       ttGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCt                                                                        >  1:4605816/1‑100 (MQ=255)
                        tGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTg                                                                       >  1:2884444/1‑100 (MQ=255)
                          cGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGaa                                                                     >  2:4208401/1‑100 (MQ=255)
                            aTTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAgg                                                                   >  2:755494/1‑100 (MQ=255)
                               cTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGttt                                                                >  1:5095940/1‑100 (MQ=255)
                               cTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGttt                                                                >  1:5052936/1‑100 (MQ=255)
                                tGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGtttt                                                               <  1:1255255/100‑1 (MQ=255)
                                tGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGtttt                                                               <  1:4153823/100‑1 (MQ=255)
                                 gCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTa                                                              <  1:3735082/100‑1 (MQ=255)
                                  cAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTAc                                                             <  1:167996/100‑1 (MQ=255)
                                   aaTATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACa                                                            <  1:3904984/100‑1 (MQ=255)
                                   aaTATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACa                                                            <  2:3212611/100‑1 (MQ=255)
                                   aaTATTTGGAAAAAAGTGGGGGAAGTTCGGCGGTTAGCGATATGTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACa                                                            <  2:2072608/100‑1 (MQ=255)
                                   aaTATTTGGAAAAAAGAGGGGGAAGTCGGTCGGTTTGCGATGTTTCTCTCACTCTGGATGTGCCGCTGTCCACGACCTTTCGCTTGCTGAAGGTTTTACa                                                            <  2:3027192/100‑1 (MQ=255)
                                    atatTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAg                                                           >  2:3969165/1‑100 (MQ=255)
                                      atTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGc                                                         <  1:1861408/100‑1 (MQ=255)
                                      atTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGc                                                         <  1:3112581/100‑1 (MQ=255)
                                      atTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGc                                                         >  1:3147576/1‑100 (MQ=255)
                                        ttGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAg                                                       >  1:3448863/1‑100 (MQ=255)
                                        ttGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAg                                                       >  2:2793453/1‑100 (MQ=255)
                                        ttGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAg                                                       >  1:4373225/1‑100 (MQ=255)
                                         tGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAAGCGGGATTTTTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGc                                                      >  2:711581/1‑100 (MQ=255)
                                          ggAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCg                                                     >  2:1494824/1‑100 (MQ=255)
                                          ggAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCg                                                     <  2:3411333/100‑1 (MQ=255)
                                           gAAAAAAGTGGGGGAAGTACGTCGGTTAGCGATATTTATCTCACTATGGCTTTGTCGCACTCCACGACCTTTCGCTTGATGATGATTTTACCGAAAGCgg                                                    <  2:2195450/100‑1 (MQ=255)
                                            aaaaaaGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGa                                                   >  2:2904715/1‑100 (MQ=255)
                                               aaaGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGAttt                                                <  2:3398991/100‑1 (MQ=255)
                                                 aGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTg                                              <  1:2178565/100‑1 (MQ=255)
                                                 aGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTg                                              <  2:4318998/100‑1 (MQ=255)
                                                 aGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTg                                              <  2:1683590/100‑1 (MQ=255)
                                                 aGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTg                                              <  1:4730535/100‑1 (MQ=255)
                                                  gTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGt                                             <  1:2927739/100‑1 (MQ=255)
                                                  gTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGt                                             <  1:293651/100‑1 (MQ=255)
                                                  gTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGt                                             <  2:312975/100‑1 (MQ=255)
                                                   tGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTc                                            <  2:2731348/100‑1 (MQ=255)
                                                   tGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTc                                            <  1:5012437/100‑1 (MQ=255)
                                                   tGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTc                                            <  1:1424048/100‑1 (MQ=255)
                                                   tGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTc                                            <  2:180522/100‑1 (MQ=255)
                                                    gggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCt                                           <  2:4065542/100‑1 (MQ=255)
                                                    gggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCt                                           >  2:278001/1‑100 (MQ=255)
                                                    gggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCt                                           >  2:3235979/1‑100 (MQ=255)
                                                     ggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTa                                          <  2:1953929/100‑1 (MQ=255)
                                                     ggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTa                                          <  2:2519991/100‑1 (MQ=255)
                                                     ggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTa                                          <  2:2399377/100‑1 (MQ=255)
                                                     ggggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTa                                          >  1:4325311/1‑100 (MQ=255)
                                                       ggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATc                                        <  2:2642816/100‑1 (MQ=255)
                                                       ggAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATc                                        <  2:3543512/100‑1 (MQ=255)
                                                        gAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCa                                       <  1:2352291/100‑1 (MQ=255)
                                                         aaGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAg                                      <  2:226294/100‑1 (MQ=255)
                                                              cgtcgGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAg                                 >  2:3687593/1‑100 (MQ=255)
                                                              cgtcgGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAg                                 <  1:3950031/100‑1 (MQ=255)
                                                               gtcgGTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGt                                >  2:622513/1‑100 (MQ=255)
                                                                  ggTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCaa                             <  1:1499616/100‑1 (MQ=255)
                                                                   gTTAGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAAt                            >  2:2026967/1‑100 (MQ=255)
                                                                      aGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAg                         <  2:157850/100‑1 (MQ=255)
                                                                      aGCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAg                         >  2:5479910/1‑100 (MQ=255)
                                                                       gCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAgg                        <  2:3096884/100‑1 (MQ=255)
                                                                       gCGATATTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAgg                        >  1:137090/1‑100 (MQ=255)
                                                                            atTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCtggt                   <  1:3899826/100‑1 (MQ=255)
                                                                            atTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCtggt                   >  2:4819575/1‑100 (MQ=255)
                                                                            atTTCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCtggt                   >  1:3589789/1‑100 (MQ=255)
                                                                             tttCTCTCAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCtggtg                  <  2:2039943/100‑1 (MQ=255)
                                                                                ctctcAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCa               <  1:645625/100‑1 (MQ=255)
                                                                                 tctcAATCTGTATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCat              <  2:4401323/100‑1 (MQ=255)
                                                                                 tctcAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCat              >  1:3607138/1‑100 (MQ=255)
                                                                                 tctcAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCat              >  2:919168/1‑100 (MQ=255)
                                                                                  ctcAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCata             >  1:4118457/1‑100 (MQ=255)
                                                                                  ctcAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGCCAATTAGGCTGGTGGCata             >  2:4906265/1‑100 (MQ=255)
                                                                                   tcAATCTGGATTTGACGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTGAATTAGGCTGGTGGCatat            <  2:5025555/100‑1 (MQ=255)
                                                                                    cAATCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCAtata           >  1:2849266/1‑100 (MQ=255)
                                                                                     aaTCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAg          >  1:1646805/1‑100 (MQ=255)
                                                                                     aaTCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAg          <  2:5287551/100‑1 (MQ=255)
                                                                                     aaTCTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAg          <  1:773984/100‑1 (MQ=255)
                                                                                        cTGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGAt       >  1:274789/1‑100 (MQ=255)
                                                                                         tGGATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGAtt      <  2:5477984/100‑1 (MQ=255)
                                                                                           gATTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAg    <  2:1974690/100‑1 (MQ=255)
                                                                                            aTTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAgg   <  1:5314340/100‑1 (MQ=255)
                                                                                            aTTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAgg   <  2:4950306/100‑1 (MQ=255)
                                                                                            aTTTGTCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAgg   <  1:671559/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGCACAGGCGTTAGAGCGGGGAATTGCGATTCTGCAATATTTGGAAAAAAGTGGGGGAAGTTCGTCGGTTAGCGATATTTCTCTCAATCTGGATTTGCCGCTCTCCACGACCTTTCGCTTGCTGAAGGTTTTACAGGCAGCGGATTTTGTCTATCAGGACAGTCAATTAGGCTGGTGGCATATAGGATTAGGT  >  NC_000913/533070‑533262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A