Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,795	A→C	F280V (TTT→GTT)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,795	0	A	C	99.4%	622.7 / ‑4.2	176	F280V (TTT→GTT)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (98/76); total (98/78)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 176 total aligned reads displayed.

CTTCATCATCGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAAAATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGT  >  NC_000913/955699‑955890

   catcatCGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAgg                                                                                           <  1:4961447/100‑1 (MQ=255)

   catcatCGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAgg                                                                                           >  1:172808/1‑100 (MQ=255)

    atcatcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGa                                                                                          >  2:1748572/1‑100 (MQ=255)

    atcatcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGATCACGGTACGTTCCAGTGCTACGCCCACATCAgaa                                                                                          >  2:190390/1‑98 (MQ=255)

    agcatcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGa                                                                                          <  2:3875951/98‑1 (MQ=255)

      catcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATg                                                                                        <  2:5310319/100‑1 (MQ=255)

       atcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgc                                                                                       >  1:5397567/1‑100 (MQ=255)

       atcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgc                                                                                       >  2:2859884/1‑100 (MQ=255)

       atcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgc                                                                                       >  1:1440655/1‑100 (MQ=255)

       atcGAAGGTTGGCGGAGCAAACACATCCAAATCTTTCAGGCCACGCCCTTGCAGCAGCTCGGTCACGTCACGTTCCAGTGCTACGACAACATCAgcctgc                                                                                       >  2:4381797/1‑95 (MQ=255)

        tcGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgcg                                                                                      >  1:2561164/1‑100 (MQ=255)

         cGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgcgc                                                                                     <  2:4749980/100‑1 (MQ=255)

         cGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgcgc                                                                                     <  2:4748549/100‑1 (MQ=255)

         cGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATgcgc                                                                                     >  1:2181880/1‑100 (MQ=255)

          gAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCa                                                                                    >  2:70429/1‑100 (MQ=255)

            aGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCAcc                                                                                  <  1:953932/100‑1 (MQ=255)

            aGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCAcc                                                                                  >  1:3230709/1‑100 (MQ=255)

             ggTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTATGTTCCAGTGCTACGCCAACATCACGATGCGCACCg                                                                                 >  2:4899784/1‑100 (MQ=255)

                 ggCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAag                                                                             <  1:3669666/100‑1 (MQ=255)

                 ggCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAag                                                                             <  1:2281292/100‑1 (MQ=255)

                    ggAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAgg                                                                          >  2:3660063/1‑100 (MQ=255)

                    ggAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAgg                                                                          >  1:2492145/1‑100 (MQ=255)

                    ggAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAgg                                                                          >  2:3490878/1‑100 (MQ=255)

                    ggAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAgg                                                                          >  2:952387/1‑100 (MQ=255)

                    ggAGTAAACACATCCAAATCTTGCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGGTGCGCACCGAAAGAgg                                                                          <  2:902668/100‑1 (MQ=255)

                     gAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGc                                                                         <  2:1421833/100‑1 (MQ=255)

                     gAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGc                                                                         <  2:1609802/100‑1 (MQ=255)

                     gAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGc                                                                         <  2:1609803/100‑1 (MQ=255)

                       gTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCaa                                                                       >  2:2448998/1‑100 (MQ=255)

                           acacAGCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGGCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCa                                                                   <  1:2379599/100‑1 (MQ=255)

                             acaTCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAgg                                                                 <  1:2389269/100‑1 (MQ=255)

                               aTCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTa                                                               >  2:562496/1‑100 (MQ=255)

                               aTCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTa                                                               >  2:507943/1‑100 (MQ=255)

                                tCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTAc                                                              >  2:746977/1‑100 (MQ=255)

                                tCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTAc                                                              >  1:1572772/1‑100 (MQ=255)

                                tCCAAATCTTTCAGGACACGACCTTGCAGCAGCTCGATCACGGTACGTTCCAGTGCTACGCCAACATCAGGCTCCCCACCGAGGGACACCAAGCCGGTAc                                                              >  2:2754334/1‑100 (MQ=255)

                                 ccAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTAcc                                                             >  1:525792/1‑100 (MQ=255)

                                  cAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTGCGCCAACATCAAGATGCGCACCGAAAGAGGCAAAGCTGGTACCg                                                            >  1:5111282/1‑100 (MQ=255)

                                  cAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCg                                                            >  1:2890294/1‑100 (MQ=255)

                                  cAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCg                                                            <  1:4202273/100‑1 (MQ=255)

                                  cAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCg                                                            >  1:274541/1‑100 (MQ=255)

                                  cAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCg                                                            <  1:705257/100‑1 (MQ=255)

                                   aaaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGt                                                           >  2:1933747/1‑100 (MQ=255)

                                   aaaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGt                                                           >  2:992501/1‑100 (MQ=255)

                                   aaaTCTTTCAGGCCACGACCTTGCAGCAGCCCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGt                                                           >  1:1219420/1‑100 (MQ=255)

                                    aaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGtt                                                          <  2:2962146/100‑1 (MQ=255)

                                    aaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGtt                                                          <  2:5083467/100‑1 (MQ=255)

                                    aaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGtt                                                          <  1:573783/100‑1 (MQ=255)

                                    aaTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGtt                                                          >  1:3879090/1‑100 (MQ=255)

                                     aTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTa                                                         <  2:200865/100‑1 (MQ=255)

                                     aTCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTa                                                         <  1:4018843/100‑1 (MQ=255)

                                      tCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGATACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAg                                                        <  2:797002/100‑1 (MQ=255)

                                       cTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGc                                                       <  1:1677846/100‑1 (MQ=255)

                                        tttCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCa                                                      >  2:2457961/1‑100 (MQ=255)

                                         ttCAGGCCACGACCTTGCAGCAGTTTGGTTATGAAACGTACGAGTGCTACGCAAACATCAGGATGCGCAGTGATACAGGCAGAGGAGCAATCGTTAGCac                                                     <  2:3149531/100‑2 (MQ=255)

                                         ttCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAg                                                     <  1:1029406/100‑1 (MQ=255)

                                         ttCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAg                                                     <  2:52777/100‑1 (MQ=255)

                                           cAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGa                                                   >  1:1606773/1‑100 (MQ=255)

                                            aGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGAt                                                  <  1:2691834/100‑1 (MQ=255)

                                              gCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTg                                                >  2:1786634/1‑100 (MQ=255)

                                               ccACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGa                                               >  1:3776087/1‑100 (MQ=255)

                                               ccACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTCCTAAGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGa                                               >  2:792401/1‑100 (MQ=255)

                                                 aCGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAAc                                             <  1:1323467/100‑1 (MQ=255)

                                                 aCGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGGACCGTTAGCAGGATTGAAc                                             <  2:5040768/100‑1 (MQ=255)

                                                 aCGACCTTGCAGCAGCGCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAAc                                             <  2:3709264/100‑1 (MQ=255)

                                                  cGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACa                                            <  1:3740149/100‑1 (MQ=255)

                                                  cGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACa                                            >  1:812921/1‑100 (MQ=255)

                                                     ccTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTa                                         >  1:3079960/1‑100 (MQ=255)

                                                      cTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTAc                                        <  1:212365/100‑1 (MQ=255)

                                                        tGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCa                                      >  1:3391114/1‑100 (MQ=255)

                                                         gcagcaGCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCAc                                     <  1:1555142/100‑1 (MQ=255)

                                                          cagcagCTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACg                                    <  2:1003263/100‑1 (MQ=255)

                                                          cagcagCTCGGTCACGGTACGTCCAAGTGTTACGCCAACATGAGGATGCGCACCGAAAGAGGCGAAGCAAGTACCGTTAGCAGGATTGAACAGTACCACg                                    <  2:2933922/100‑1 (MQ=255)

                                                           agcagcTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGc                                   >  2:3824467/1‑100 (MQ=255)

                                                            gcagcTCGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCa                                  >  1:2029018/1‑100 (MQ=255)

                                                                  cGGTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCAc                            >  1:5451566/1‑100 (MQ=255)

                                                                   ggTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCAcc                           >  1:3875578/1‑100 (MQ=255)

                                                                   ggTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCAcc                           >  1:1578615/1‑100 (MQ=255)

                                                                    gTCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCg                          >  2:1248716/1‑100 (MQ=255)

                                                                     tCACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCgg                         <  2:2159187/100‑1 (MQ=255)

                                                                      cACGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGGACCACGCAAATCACCGGa                        >  1:1017125/1‑100 (MQ=255)

                                                                       aCGGTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGAt                       <  1:4877471/100‑1 (MQ=255)

                                                                         ggTACGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATAc                     <  2:3365568/100‑1 (MQ=255)

                                                                            aCGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTgg                  >  1:3020167/1‑100 (MQ=255)

                                                                             cGTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGgc                 >  1:3634176/1‑100 (MQ=255)

                                                                              gTTCCAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGgcc                <  2:800774/100‑1 (MQ=255)

                                                                               ttCCAGTGCTACGCCAACATCAGGATGTGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGgccg               >  2:2492702/1‑100 (MQ=255)

                                                                                 ccAGTGCTACGCCAACATCACGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGgccgcc             <  1:5221355/100‑1 (MQ=255)

                                                                                  cAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCa            >  2:4639763/1‑100 (MQ=255)

                                                                                  cAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCa            >  1:4389949/1‑100 (MQ=255)

                                                                                  cAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCa            >  1:1404132/1‑100 (MQ=255)

                                                                                  cAGTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCa            >  1:4329748/1‑100 (MQ=255)

                                                                                    gTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCCAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAg          >  2:2643452/1‑100 (MQ=255)

                                                                                    gTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAg          >  1:1901649/1‑100 (MQ=255)

                                                                                    gTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAg          >  1:2121046/1‑100 (MQ=255)

                                                                                    gTGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAg          >  1:99351/1‑100 (MQ=255)

                                                                                     tGCTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGc         <  1:28404/100‑1 (MQ=255)

                                                                                       cTACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGCGa       >  1:1754739/1‑100 (MQ=255)

                                                                                        tACGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGCGaa      >  1:1122829/1‑100 (MQ=255)

                                                                                         aCGCCAACATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGCGAAc     <  1:3445447/100‑1 (MQ=255)

CTTCATCATCGAAGGTTGGCGGAGTAAACACATCCAAATCTTTCAGGCCACGACCTTGCAGCAGCTCGGTCACGGTACGTTCCAGTGCTACGCCAAAATCAGGATGCGCACCGAAAGAGGCAAAGCAGGTACCGTTAGCAGGATTGAACAGTACCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGT  >  NC_000913/955699‑955890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A