Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 979,391 T→G A1022A (GCT→GCG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913979,3910TG99.1% 790.0 / ‑3.7 219A1022A (GCT→GCG) mukBchromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (109/108);  total (111/108)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 219 total aligned reads displayed.

GTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAA  >  NC_000913/979294‑979487
                                                                                                 |                                                                                                
gTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTc                                                                                                <  1:1730288/100‑1 (MQ=255)
    aCGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGTGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTg                                                                                            <  2:671608/100‑1 (MQ=255)
     cGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGa                                                                                           >  1:5065444/1‑100 (MQ=255)
     cGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGa                                                                                           >  1:897389/1‑100 (MQ=255)
      gTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaa                                                                                          >  1:1012486/1‑100 (MQ=255)
      gTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaa                                                                                          >  2:2910299/1‑100 (MQ=255)
      gTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaa                                                                                          <  2:4484001/100‑1 (MQ=255)
       tCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaaa                                                                                         <  2:4090531/100‑1 (MQ=255)
        cTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaaaa                                                                                        <  1:1318935/100‑1 (MQ=255)
        cTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaaaa                                                                                        >  1:2281671/1‑100 (MQ=255)
        cTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGaaaa                                                                                        >  2:1442097/1‑100 (MQ=255)
         tGGAACAGGCGGCAGCGGAGCGGACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGGCGCTGAAAAg                                                                                       <  2:2061121/100‑1 (MQ=255)
          ggAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGt                                                                                      >  2:4795260/1‑100 (MQ=255)
           gAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAgag                                                                                     >  2:4397538/1‑98 (MQ=255)
            aaCAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGGGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTc                                                                                    <  2:3653509/100‑1 (MQ=255)
            aaCAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTc                                                                                    >  1:2563199/1‑100 (MQ=255)
            aaCAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTc                                                                                    <  2:145719/100‑1 (MQ=255)
             ncAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCt                                                                                   >  1:3104376/2‑100 (MQ=255)
             aCAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCt                                                                                   >  2:5052233/1‑100 (MQ=255)
               aGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTa                                                                                 <  1:3834855/100‑1 (MQ=255)
               aGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTa                                                                                 <  2:5080549/100‑1 (MQ=255)
               aGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTa                                                                                 <  1:1948828/100‑1 (MQ=255)
                ggCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTAc                                                                                <  2:1366549/100‑1 (MQ=255)
                ggCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTAc                                                                                >  1:4316823/1‑100 (MQ=255)
                ggCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTAc                                                                                <  2:4783701/100‑1 (MQ=255)
                 gCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACg                                                                               >  1:4619745/1‑100 (MQ=255)
                 gCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACg                                                                               <  2:1502705/100‑1 (MQ=255)
                  cGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGa                                                                              >  1:217486/1‑100 (MQ=255)
                  cGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGa                                                                              <  1:1863736/100‑1 (MQ=255)
                   ggAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGac                                                                             >  1:3044988/1‑100 (MQ=255)
                   ggAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGac                                                                             >  2:1799105/1‑100 (MQ=255)
                      aGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACAcc                                                                          >  2:2485110/1‑100 (MQ=255)
                       gCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCa                                                                         >  2:5440963/1‑100 (MQ=255)
                       gCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCa                                                                         <  1:1637422/100‑1 (MQ=255)
                        cGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCaa                                                                        >  2:4133218/1‑100 (MQ=255)
                         ggAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCaaa                                                                       >  2:1517295/1‑100 (MQ=255)
                          gAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCaaaa                                                                      <  2:4281814/100‑1 (MQ=255)
                            gCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCaaaaaa                                                                    >  1:1934649/1‑100 (MQ=255)
                               tACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGCCACCAAAAAagag                                                                 <  1:3987024/100‑1 (MQ=255)
                               tACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAagag                                                                 <  1:1349798/100‑1 (MQ=255)
                                 cccGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCt                                                               >  1:789150/1‑100 (MQ=255)
                                 cccGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCt                                                               >  1:1727095/1‑100 (MQ=255)
                                 cccGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGCTGAGTCAGTAGAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCt                                                               >  1:152829/1‑100 (MQ=255)
                                  cagcgcTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTa                                                              <  2:5270404/98‑1 (MQ=255)
                                   cgcgCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGACGCTGAAAAGGTCTAACGACACC‑AAAAAGAGCTACt                                                            >  2:5199405/1‑100 (MQ=255)
                                     cgcTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTc                                                           >  2:5371698/1‑100 (MQ=255)
                                     cgcTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCATAAAAAAAAAAAAAAGAGCTACTc                                                           <  2:2541181/100‑1 (MQ=255)
                                       cTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCaa                                                         >  1:1054051/1‑100 (MQ=255)
                                        tCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAAc                                                        <  1:1660144/100‑1 (MQ=255)
                                         ngcgAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACg                                                       >  1:4441933/2‑100 (MQ=255)
                                         cgcgAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACg                                                       >  1:251398/1‑100 (MQ=255)
                                         cgcgAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTAAGACACCAAAAAAGAGCTACTCAACg                                                       >  2:4360314/1‑100 (MQ=255)
                                            gAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAATAGCTACTCAACGATc                                                    >  1:3626781/1‑100 (MQ=255)
                                              aGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTg                                                  <  2:4892873/100‑1 (MQ=255)
                                              aGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTg                                                  <  1:3339811/100‑1 (MQ=255)
                                               gCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGc                                                 <  1:4905307/100‑1 (MQ=255)
                                                cGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCa                                                <  1:4377067/100‑1 (MQ=255)
                                                 gTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCaa                                               >  2:2672747/1‑100 (MQ=255)
                                                  ttGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc                                              <  2:1646973/100‑1 (MQ=255)
                                                  ttGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc                                              <  1:1461113/100‑1 (MQ=255)
                                                     cgcgGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg                                           >  1:3219135/1‑100 (MQ=255)
                                                      gcgGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGa                                          <  1:2855814/100‑1 (MQ=255)
                                                        ggTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGAAACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt                                        <  2:555681/100‑1 (MQ=255)
                                                         gTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAtt                                       <  1:209778/100‑1 (MQ=255)
                                                            aCGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCa                                    <  1:950495/100‑1 (MQ=255)
                                                              gcagcgcagTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACAACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAgg                                  <  2:3775613/100‑1 (MQ=255)
                                                              gcagcgcagTGGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAATTTCTTACGACATCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAgg                                  <  2:2604277/100‑1 (MQ=255)
                                                               cagcgcagTTGAGTCAGTACAACCAGGTGATGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGa                                 <  2:296957/100‑1 (MQ=255)
                                                                agcgcagTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGat                                >  1:2948247/1‑100 (MQ=255)
                                                                 gcgcagTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGata                               >  1:774228/1‑100 (MQ=255)
                                                                   gcagTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATc                             >  1:2917589/1‑100 (MQ=255)
                                                                    cagTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCg                            <  1:2329693/100‑1 (MQ=255)
                                                                      gTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCTACGATCTGCAACGTGAATTGCAGGATATCGgc                          >  1:5098309/1‑100 (MQ=255)
                                                                      gTTGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgc                          >  2:1465003/1‑100 (MQ=255)
                                                                        tGAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTATGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgcgt                        >  2:5231095/1‑100 (MQ=255)
                                                                         gAGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgcgtg                       <  2:4250289/100‑1 (MQ=255)
                                                                          aGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgcgtgc                      >  1:1197858/1‑100 (MQ=255)
                                                                          aGTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgcgtgc                      >  1:3300694/1‑100 (MQ=255)
                                                                           gTCAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGgcgtgcg                     >  2:1556077/1‑100 (MQ=255)
                                                                             cAGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg                   <  2:2374098/100‑1 (MQ=255)
                                                                              aGTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCgtgcgtgc                  >  1:846342/1‑100 (MQ=255)
                                                                               gTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt                 <  1:1819646/100‑1 (MQ=255)
                                                                               gTACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt                 <  1:2706749/100‑1 (MQ=255)
                                                                                tACAACCTGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTCCTCAACGATCTGCAACGTGAATTGCAGGATATCGTCGTGCGTGCTg                <  2:5101318/100‑1 (MQ=255)
                                                                                tACAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTg                >  1:4072926/1‑100 (MQ=255)
                                                                                  cAACCAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGAt              <  2:2199160/100‑1 (MQ=255)
                                                                                     ccAGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGc           <  1:3938446/100‑1 (MQ=255)
                                                                                       aGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCgg         <  1:2193160/100‑1 (MQ=255)
                                                                                       aGGTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCgg         <  2:1213475/100‑1 (MQ=255)
                                                                                        ggTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCggg        >  1:4186686/1‑100 (MQ=255)
                                                                                        ggTGCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCggg        <  1:757332/100‑1 (MQ=255)
                                                                                        ggTGCTGGCGTCGCTGAAAAGTCCTTACGACACCAAAAAAGAGCTCCTCAAAGATCTGCAACGTGAATTGCAGGATATCGGCGTGAGTGCTGAGATCgga        >  2:1103268/1‑99 (MQ=255)
                                                                                           gCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCa     <  2:140427/100‑1 (MQ=255)
                                                                                           gCTGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCa     <  1:5414998/100‑1 (MQ=255)
                                                                                           gCTGGCGTCGCTGAAAAGTTCTTACAACACCAAAAAAGAGCTACTCTACGATCTGCAACGTGAATGGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCa     <  1:5168247/100‑1 (MQ=255)
                                                                                             tGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCaga   >  2:1270599/1‑100 (MQ=255)
                                                                                             tGGCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCaga   <  2:3945699/100‑1 (MQ=255)
                                                                                              ggCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCagaa  >  1:2875290/1‑100 (MQ=255)
                                                                                              ggCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCagaa  >  2:3292891/1‑100 (MQ=255)
                                                                                              ggCGTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCagaa  >  1:4740820/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTGAACGTCTGGAACAGGCGGAAGCGGAGCGTACCCGCGCTCGCGAAGCGTTGCGCGGTCACGCAGCGCAGTTGAGTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAA  >  NC_000913/979294‑979487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A