Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,192,931 C→T R281R (CGG→CGA)  mnmA ← tRNA(Gln,Lys,Glu) U34 2‑thiouridylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,192,9310CT98.4% 640.7 / ‑1.9 191R281R (CGG→CGA) mnmAtRNA(Gln,Lys,Glu) U34 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (67/118);  total (68/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 191 total aligned reads displayed.

CGATAGCGGGTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCC  >  NC_000913/1192836‑1193026
                                                                                               |                                                                                               
cGATAGCGGGTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTcgcg                                                                                             >  1:2630592/1‑100 (MQ=255)
  aTAGCGGGTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCggg                                                                                           <  1:113901/100‑1 (MQ=255)
  aTAGCGCGTTTTTACCGTGCAACGCATAGTTCCGGTGAATGGTTCTCGATCGACCCAGTCCAACTGCTGGGCAATCAACCCGACAGACATCAGACGCggg                                                                                           <  2:5172921/100‑1 (MQ=255)
    aGCGGGTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGgtg                                                                                         <  1:5084795/100‑1 (MQ=255)
        ggTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCa                                                                                     >  1:2290358/1‑100 (MQ=255)
        ggTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCa                                                                                     <  1:2364524/100‑1 (MQ=255)
         gTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCAt                                                                                    <  1:1467680/100‑1 (MQ=255)
         gTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCAt                                                                                    <  1:2957914/100‑1 (MQ=255)
         gTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCAt                                                                                    <  2:66467/100‑1 (MQ=255)
         gTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCAt                                                                                    <  1:4252103/100‑1 (MQ=255)
         gTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCAt                                                                                    <  2:4966208/100‑1 (MQ=255)
          tttttACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATg                                                                                   <  2:3039005/100‑1 (MQ=255)
          tttttACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATg                                                                                   >  2:1576969/1‑100 (MQ=255)
            tttGCCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGGGCAACTGCTGGGCAATCAACCCGACATACATCAGTCGCGGGTGGTCATGGc                                                                                 <  2:2814303/100‑1 (MQ=255)
            tttACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGc                                                                                 <  1:2260685/100‑1 (MQ=255)
            tttACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGc                                                                                 <  2:1076869/100‑1 (MQ=255)
             ttACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGcc                                                                                >  2:2015348/1‑100 (MQ=255)
              tACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGccc                                                                               <  2:4700545/100‑1 (MQ=255)
               aCCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCt                                                                              <  2:710997/100‑1 (MQ=255)
                ccGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTg                                                                             <  1:302818/100‑1 (MQ=255)
                  gTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAg                                                                           >  1:201209/1‑100 (MQ=255)
                    gCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCg                                                                         <  1:3312000/100‑1 (MQ=255)
                    gCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCg                                                                         <  2:3902044/100‑1 (MQ=255)
                      aaCGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGAc                                                                       <  2:4747967/100‑1 (MQ=255)
                      aaCGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGAc                                                                       <  1:3155849/100‑1 (MQ=255)
                       aCGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACa                                                                      <  1:4785115/100‑1 (MQ=255)
                          cATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAAcc                                                                   <  2:2821661/100‑1 (MQ=255)
                          cATAGTACCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAAcc                                                                   >  1:4922413/1‑100 (MQ=255)
                           aTAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCa                                                                  >  1:4899139/1‑100 (MQ=255)
                            tAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAg                                                                 <  1:432747/100‑1 (MQ=255)
                             aGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGATCATGGCCCTGAGCGACAACCAGa                                                                >  1:4058835/1‑100 (MQ=255)
                               tGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAt                                                              >  1:1745906/1‑100 (MQ=255)
                               tGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAt                                                              <  1:5229776/100‑1 (MQ=255)
                                tgcGGTGAATGGTTCGCGATCGACGCAGGGTAAGTGCTGGGCAATTAGCCGGGCAGACATCAGTCGCGGGTGTTCATGGCGCTGGGCGACATGCAGAAtg                                                             <  2:197901/98‑1 (MQ=255)
                                 ccGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgt                                                            <  2:421450/100‑1 (MQ=255)
                                 ccGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgt                                                            <  2:901127/100‑1 (MQ=255)
                                   ggTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgttg                                                          <  2:3929154/100‑1 (MQ=255)
                                   ggTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTAGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgttg                                                          >  2:2622988/1‑100 (MQ=255)
                                    gTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgttgt                                                         <  1:4300271/100‑1 (MQ=255)
                                    gTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAAtgttgt                                                         >  2:310423/1‑100 (MQ=255)
                                      gAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGttt                                                       <  2:2277276/100‑1 (MQ=255)
                                      gAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGttt                                                       >  1:3932202/1‑100 (MQ=255)
                                      gAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGttt                                                       <  1:3344299/100‑1 (MQ=255)
                                        aTGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTc                                                     <  1:1689381/100‑1 (MQ=255)
                                         tGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCg                                                    <  1:1288544/100‑1 (MQ=255)
                                          ggTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGa                                                   >  2:3703953/1‑100 (MQ=255)
                                          ggTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGa                                                   >  2:919304/1‑100 (MQ=255)
                                          ggTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGa                                                   >  1:5554653/1‑100 (MQ=255)
                                           gTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGAc                                                  <  1:127133/100‑1 (MQ=255)
                                           gTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGAc                                                  <  1:5091045/100‑1 (MQ=255)
                                           gTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGAc                                                  >  2:1620095/1‑100 (MQ=255)
                                           gTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGAc                                                  <  2:4139507/100‑1 (MQ=255)
                                                cgATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCtt                                             <  2:3174457/100‑1 (MQ=255)
                                                cgATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCtt                                             <  1:2263067/100‑1 (MQ=255)
                                                cgATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCtt                                             <  2:4034606/100‑1 (MQ=255)
                                                 gATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCttt                                            >  2:3536230/1‑100 (MQ=255)
                                                   tCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGt                                          <  1:4955430/100‑1 (MQ=255)
                                                    cGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTc                                         >  1:862314/1‑100 (MQ=255)
                                                     gACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTcc                                        >  2:124398/1‑100 (MQ=255)
                                                      aCCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTcca                                       >  2:4819943/1‑100 (MQ=255)
                                                      aCCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTcca                                       <  2:4275650/100‑1 (MQ=255)
                                                      aCCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTcca                                       >  1:3420412/1‑100 (MQ=255)
                                                        ccAGTGCAACTGCTGGGCCATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTccacc                                     <  1:1117226/100‑1 (MQ=255)
                                                        ccAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTccacc                                     <  1:3670804/100‑1 (MQ=255)
                                                         cAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTccacca                                    <  1:644419/100‑1 (MQ=255)
                                                          aGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTccaccan                                   <  2:5376367/100‑2 (MQ=255)
                                                          aGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCcaccac                                   >  1:2225821/1‑100 (MQ=255)
                                                           gTGCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTCGTTTTCGACGTCTTTGTCCACcaca                                  >  1:4233745/1‑100 (MQ=255)
                                                             gCAACTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTACACCACATa                                <  1:5428222/100‑1 (MQ=255)
                                                               aaCTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATAcc                              <  2:2525150/100‑1 (MQ=255)
                                                               aaCTGCTGGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATAcc                              <  1:2030887/100‑1 (MQ=255)
                                                                 ctgctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCAc                            >  1:4496943/1‑100 (MQ=255)
                                                                 ctgctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCAc                            <  2:840589/100‑1 (MQ=255)
                                                                  tgctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACg                           <  2:1519874/100‑1 (MQ=255)
                                                                  tgctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACg                           <  1:4695934/100‑1 (MQ=255)
                                                                   gctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACgg                          >  1:3611240/1‑100 (MQ=255)
                                                                    ctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGt                         <  1:3981428/100‑1 (MQ=255)
                                                                    ctgGGCAATCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGt                         <  1:3123564/100‑1 (MQ=255)
                                                                         cAATGAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGttcttc                    >  2:4676086/1‑100 (MQ=255)
                                                                          aaTCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCg                   <  2:592825/100‑1 (MQ=255)
                                                                           aTCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCgg                  <  1:343487/100‑1 (MQ=255)
                                                                           aTCAACCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCgg                  >  2:3088878/1‑100 (MQ=255)
                                                                            tCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGt                 >  2:189120/1‑100 (MQ=255)
                                                                              aaCCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGtcc               >  2:4392172/1‑98 (MQ=255)
                                                                              aaCCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTAc               <  2:3996068/100‑1 (MQ=255)
                                                                              aaCCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTAc               <  2:186983/100‑1 (MQ=255)
                                                                               aCCCGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTAcc              <  2:1321800/100‑1 (MQ=255)
                                                                                cccGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACct             <  1:931554/100‑1 (MQ=255)
                                                                                cccGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACct             <  1:3738297/100‑1 (MQ=255)
                                                                                cccGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACct             >  1:2746321/1‑100 (MQ=255)
                                                                                cccGACAGACATCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACct             >  1:4557478/1‑100 (MQ=255)
                                                                                     cagacaTCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCttt        <  2:3436357/100‑1 (MQ=255)
                                                                                      agacaTCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTg       <  2:4022004/100‑1 (MQ=255)
                                                                                       gacaGCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTgg      <  2:2222636/100‑1 (MQ=255)
                                                                                        agaTCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTCGTTTTGGACGTCTTTGTCCACCACATGCCACGGCTCTTCGGTACCTTCTTTGGt     <  2:5480812/98‑1 (MQ=255)
                                                                                        acaTCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGt     >  2:2862261/1‑100 (MQ=255)
                                                                                         caTCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTg    <  1:3781363/100‑1 (MQ=255)
                                                                                           tCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGcc  >  2:1745804/1‑100 (MQ=255)
                                                                                           tCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGcc  >  1:3538584/1‑100 (MQ=255)
                                                                                           tCAGTCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGcc  <  1:1507096/100‑1 (MQ=255)
                                                                                               |                                                                                               
CGATAGCGGGTTTTTACCGTGCAACGCATAGTGCCGGTGAATGGTTCGCGATCGACCCAGTGCAACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTTTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCC  >  NC_000913/1192836‑1193026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A