Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,244,222 G→A T102T (ACC→ACT)  ycgR ← flagellar velocity braking protein, c‑di‑GMP‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,244,2220GA97.4% 520.7 / 0.7 155T102T (ACC→ACT) ycgRflagellar velocity braking protein, c‑di‑GMP‑regulated
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (77/74);  total (79/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 171 total aligned reads displayed.

CCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATG  >  NC_000913/1244125‑1244318
                                                                                                 |                                                                                                
ccGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtacagt                                                                                                <  1:3854955/100‑1 (MQ=255)
ccGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtacagt                                                                                                >  2:1689814/1‑100 (MQ=255)
 cgcgAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtacagta                                                                                               >  1:4281819/1‑100 (MQ=255)
 cGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtacagta                                                                                               >  2:3317279/1‑100 (MQ=255)
 cGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtacagta                                                                                               >  1:488329/1‑100 (MQ=255)
  gCCAGTTTGGTCTGGGAAAAATAAGGCGGAAGGAGTGGGACGGAGATGCGGAAATATGGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGtaa                                                                                              >  1:4848722/1‑100 (MQ=255)
    cAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGtaata                                                                                            <  2:204531/100‑1 (MQ=255)
    cAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGtaata                                                                                            <  2:4725474/100‑1 (MQ=255)
    cAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGtaata                                                                                            <  1:521778/100‑1 (MQ=255)
      gTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGCGGCGGTACAGTAATaaa                                                                                          >  1:5124035/1‑100 (MQ=255)
      gTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATaaa                                                                                          >  2:4407632/1‑100 (MQ=255)
      gTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATaaa                                                                                          >  1:139051/1‑100 (MQ=255)
        ttGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATg                                                                                        <  2:1694226/100‑1 (MQ=255)
         tGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGc                                                                                       >  2:1235061/1‑100 (MQ=255)
          ggTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGcc                                                                                      <  2:3508721/100‑1 (MQ=255)
          ggTCTGGAAAAAATAAGGCGGATGGAGTGGGGAGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGcc                                                                                      <  2:249304/100‑1 (MQ=255)
           gTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCg                                                                                     <  1:3348442/100‑1 (MQ=255)
            tCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCgg                                                                                    <  1:4012299/100‑1 (MQ=255)
             cTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGa                                                                                   >  1:1052451/1‑100 (MQ=255)
             cTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATAGCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGa                                                                                   >  2:2490398/1‑100 (MQ=255)
              tGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGaa                                                                                  >  1:5291623/1‑100 (MQ=255)
               ggCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAg                                                                                 >  1:2620990/1‑100 (MQ=255)
               ggCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAg                                                                                 >  1:2947026/1‑100 (MQ=255)
                gCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGc                                                                                <  1:3236540/100‑1 (MQ=255)
                 cAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCt                                                                               >  1:3233580/1‑100 (MQ=255)
                  aaaaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTg                                                                              >  2:4312629/1‑100 (MQ=255)
                   aaaaTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGc                                                                             <  1:1907800/100‑1 (MQ=255)
                      aTAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAg                                                                          <  1:179222/100‑1 (MQ=255)
                           gCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTc                                                                     <  1:1227074/100‑1 (MQ=255)
                            cGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCa                                                                    >  2:3911194/1‑100 (MQ=255)
                            cGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCa                                                                    >  2:3275481/1‑100 (MQ=255)
                             ggATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCAc                                                                   <  2:481273/100‑1 (MQ=255)
                                tGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCActct                                                                >  2:3199061/1‑100 (MQ=255)
                                 ggAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTctg                                                               >  2:5382554/1‑100 (MQ=255)
                                  gAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTctgc                                                              >  1:2942051/1‑100 (MQ=255)
                                  gAGTGGGGCGGAGATGCGGAAATATCGGCGTCGGTGTACAAACCGTAAGGTGGGAGGCGGAACAGTAATAAAGGCCGGAAGCTGCAAGGATTCACTctgc                                                              >  2:1945446/1‑100 (MQ=255)
                                  gAGTGGGGCGGAGAGGCGGAAATATCGGCGTCGTTGTACAAACCATAATGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTctgc                                                              <  1:2547942/100‑1 (MQ=255)
                                    gTGGGGCGGAGGTACGGAAAAATCGGCGTCGTGGTACAAACCATTAGGTGTGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTctgctg                                                            <  2:956931/100‑1 (MQ=255)
                                        ggCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGTCGGAAGCTGCAAGTATTCACTCTGCTGTAgt                                                        >  2:1979605/1‑100 (MQ=255)
                                        ggCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAgt                                                        <  2:3864134/100‑1 (MQ=255)
                                         gCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAgtt                                                       <  2:482895/100‑1 (MQ=255)
                                          cGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAgttg                                                      >  1:5386913/1‑100 (MQ=255)
                                              gaTGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCa                                                  <  2:2044015/100‑1 (MQ=255)
                                               aTGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCaa                                                 <  1:846677/100‑1 (MQ=255)
                                                tGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAAc                                                <  1:4062127/100‑1 (MQ=255)
                                                tGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGCGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAAc                                                >  2:2102676/1‑100 (MQ=255)
                                                  cGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAg                                              >  1:2693718/1‑100 (MQ=255)
                                                  cGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAg                                              <  1:1251679/100‑1 (MQ=255)
                                                  cGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAg                                              >  1:3708152/1‑100 (MQ=255)
                                                  cGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTAACTCTGCTGTAGTTGTTCAACAg                                              >  2:797061/1‑100 (MQ=255)
                                                    gAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTa                                            <  2:3268335/100‑1 (MQ=255)
                                                       atatCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAAc                                         <  2:586285/100‑1 (MQ=255)
                                                       atatCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAAc                                         <  2:615719/100‑1 (MQ=255)
                                                        tatCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACt                                        >  1:112486/1‑100 (MQ=255)
                                                         atCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTc                                       <  2:4242597/100‑1 (MQ=255)
                                                         atCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTc                                       >  1:3918942/1‑100 (MQ=255)
                                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCg                                      <  1:1531290/100‑1 (MQ=255)
                                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCg                                      <  2:2709429/100‑1 (MQ=255)
                                                          tCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCg                                      <  1:1748839/100‑1 (MQ=255)
                                                            ggCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGAc                                    <  2:3754670/100‑1 (MQ=255)
                                                            ggCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGAc                                    >  2:1095260/1‑100 (MQ=255)
                                                               gtcgtTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACttt                                 >  1:4096629/1‑100 (MQ=255)
                                                                tctttGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTc                                <  2:3431321/97‑1 (MQ=255)
                                                                  gtTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGc                              >  2:2929032/1‑100 (MQ=255)
                                                                  gtTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGc                              >  1:3645296/1‑100 (MQ=255)
                                                                  gtTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGc                              >  1:1295470/1‑100 (MQ=255)
                                                                  gtTGTACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGc                              >  1:5531704/1‑100 (MQ=255)
                                                                      tACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAGGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCAccc                          <  2:5077459/100‑1 (MQ=255)
                                                                      tACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCAccc                          <  2:2704671/100‑1 (MQ=255)
                                                                      tACAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCAccc                          >  2:526892/1‑100 (MQ=255)
                                                                       aCAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCt                         <  2:4912842/100‑1 (MQ=255)
                                                                       aCAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCt                         <  1:3893967/100‑1 (MQ=255)
                                                                       aCAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCt                         >  1:1918340/1‑100 (MQ=255)
                                                                       aCAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCt                         >  1:2060487/1‑100 (MQ=255)
                                                                        cAAACCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTg                        >  2:5223302/1‑100 (MQ=255)
                                                                          acggaTAAGGTGGGAGGCGGTGCAGTAATAAATGCAGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCGTGAg                      <  2:4641205/96‑1 (MQ=255)
                                                                          aaCCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAg                      >  1:2386734/1‑100 (MQ=255)
                                                                          aaCCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAg                      >  1:4274715/1‑100 (MQ=255)
                                                                           aCCATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGt                     >  1:2103185/1‑100 (MQ=255)
                                                                            ccATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGtt                    >  1:2596679/1‑100 (MQ=255)
                                                                            ccATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGtt                    >  2:405151/1‑100 (MQ=255)
                                                                             cATAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCCACAGTAAACTCGACTTTCGCACCCTGAGttt                   >  1:50122/1‑100 (MQ=255)
                                                                              aTAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAGACTCGACTTTCGCACCCTGAGTTTc                  >  2:4366403/1‑100 (MQ=255)
                                                                              aTAAGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACGGTAAACTCGACTTTCGCACCCTGAGTTTc                  >  1:1333180/1‑100 (MQ=255)
                                                                                aaGGTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcgg                <  2:4206416/100‑1 (MQ=255)
                                                                                  ggTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggcg              >  1:3716641/1‑100 (MQ=255)
                                                                                   gTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggcgg             >  1:4159690/1‑100 (MQ=255)
                                                                                   gTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggcgg             <  2:1596242/100‑1 (MQ=255)
                                                                                   gTGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTcggcgg             <  1:2845757/100‑1 (MQ=255)
                                                                                    tGGGAGGCGGTACAGTAATAAGTGCCGGAAGCTGCAAGTGTTCACTCTGCTGTAGTTGTGCAACAGTAAACGCGACTTTCGCACCCTGAGTTTCGGCggt            <  1:5390972/100‑1 (MQ=255)
                                                                                    tGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggt            >  1:1677345/1‑100 (MQ=255)
                                                                                    tGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggt            <  1:1507134/100‑1 (MQ=255)
                                                                                    tGGGAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCGGTAGTTGCTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggt            <  1:5282492/100‑1 (MQ=255)
                                                                                      ggAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGCCTTTCGCACCCTGAGTTTCGGCggtaa          >  1:4480016/1‑100 (MQ=255)
                                                                                       gAGGCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaat         <  1:1041876/100‑1 (MQ=255)
                                                                                         ggCGGTACAGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatgg       >  2:4540427/1‑100 (MQ=255)
                                                                                           cggtacagtaATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggta     >  2:2548995/1‑100 (MQ=255)
                                                                                           cggtacagtaATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggta     >  1:2399443/1‑100 (MQ=255)
                                                                                            ggtacagtaATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaatggtaa    <  1:409136/100‑1 (MQ=255)
                                                                                             gtacagtaATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCACCACTAAACTCGACTTTCGCACCCTGAGTTTCGGCggtaagggtaat   >  1:3082552/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTACGGTAATAAATGCCGGAAGCTGCAAGTATTCACTCTGCTGTAGTTGTTCAACAGTAAACTCGACTTTCGCACCCTGAGTTTCGGCGGTAATGGTAATG  >  NC_000913/1244125‑1244318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A