Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,605,170 A→C G40G (GGA→GGC)  lsrB → autoinducer 2‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,605,1700AC98.5% 723.4 / ‑0.8 203G40G (GGA→GGC) lsrBautoinducer 2‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (83/117);  total (85/118)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 203 total aligned reads displayed.

AAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGT  >  NC_000913/1605073‑1605266
                                                                                                 |                                                                                                
aaaTCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCtt                                                                                                <  1:4406065/100‑1 (MQ=255)
 aaTTGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTCTGAATGTGGAGGCCGCAGAGCGCATTGCCTTTATACCCAAACTGGTTGGCGGGGGCttt                                                                                               >  2:5054732/1‑100 (MQ=255)
  aTCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCtttt                                                                                              <  2:2651435/100‑1 (MQ=255)
   tCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCttttt                                                                                             <  2:1642714/100‑1 (MQ=255)
   tCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCttttt                                                                                             <  1:4247169/100‑1 (MQ=255)
    cGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCtttttt                                                                                            <  2:2895226/100‑1 (MQ=255)
    cGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCtttttt                                                                                            <  2:2733143/100‑1 (MQ=255)
    cGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCtttttt                                                                                            <  1:941325/100‑1 (MQ=255)
     gCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTa                                                                                           <  2:2780303/100‑1 (MQ=255)
     gCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTa                                                                                           <  1:2538068/100‑1 (MQ=255)
      ccTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTAc                                                                                          <  2:142585/100‑1 (MQ=255)
      ccTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTAc                                                                                          <  2:141371/100‑1 (MQ=255)
       cttacttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACCGGTTGGCGTGGGCTTTTTTAcc                                                                                         <  2:2508466/100‑1 (MQ=255)
          acttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGc                                                                                      <  2:4518118/100‑1 (MQ=255)
          acttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGc                                                                                      <  2:1710519/100‑1 (MQ=255)
           cttaGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCg                                                                                     >  1:2789478/1‑100 (MQ=255)
              aGCGCTCTTGGCATTACCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTg                                                                                  <  1:3183874/100‑1 (MQ=255)
               gcgcTCTTGGCATTGCCGCAATCTCTAGGAATGTGCAGGCCGCAGAGCGTATTGCATTTCTTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTgg                                                                                 <  1:4830538/100‑1 (MQ=255)
                cgcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGc                                                                                <  2:1025351/100‑1 (MQ=255)
                 gcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCa                                                                               <  2:8724/100‑1 (MQ=255)
                 gcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCa                                                                               >  1:5184772/1‑100 (MQ=255)
                  ctctTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCaa                                                                              >  2:4809570/1‑100 (MQ=255)
                   tctTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAAc                                                                             <  2:4062942/100‑1 (MQ=255)
                     ttGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACgg                                                                           <  1:715983/100‑1 (MQ=255)
                         cATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGca                                                                       >  1:3115091/1‑100 (MQ=255)
                         cATTGCCGCAATCTCTATGAATGTGCAAGCCGCAGAGCGTATTGCATTTATTCCCAAACCGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGca                                                                       >  1:4917931/1‑100 (MQ=255)
                          aTTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGcac                                                                      <  1:302378/100‑1 (MQ=255)
                             gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCacaac                                                                   <  2:2658742/100‑1 (MQ=255)
                              ccGCGATCTTTATGAATGTTCTGGCCGCAGAGCGTATTGCATTGATTGCCAACCTGGTTGGCGTAGGCCTGTTTACCAGGCGCGGCAACGGAGCctaaca                                                                  >  2:1001038/1‑100 (MQ=255)
                              ccGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCacaaca                                                                  >  1:3771226/1‑100 (MQ=255)
                               cGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCAcaacaa                                                                 >  1:1775442/1‑100 (MQ=255)
                                gCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAg                                                                >  1:1325328/1‑100 (MQ=255)
                                gCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAg                                                                <  2:3582661/100‑1 (MQ=255)
                                    tctcTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCggg                                                            <  1:4541036/100‑1 (MQ=255)
                                    tctcTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCggg                                                            <  2:4416320/100‑1 (MQ=255)
                                     ctctATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGt                                                           >  2:5326884/1‑100 (MQ=255)
                                      tctATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTa                                                          <  2:760670/100‑1 (MQ=255)
                                      tctATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTa                                                          <  1:3325716/100‑1 (MQ=255)
                                      tctATGAATGTGCAGGCCGCAGAGCGGATTGCATTTATTCCCAGACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTa                                                          <  2:3010828/100‑1 (MQ=255)
                                         aTGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAag                                                       <  1:3140344/100‑1 (MQ=255)
                                         aTGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAag                                                       <  2:4581954/100‑1 (MQ=255)
                                          tGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAaga                                                      >  2:5015962/1‑100 (MQ=255)
                                           gAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAagag                                                     >  2:4559355/1‑100 (MQ=255)
                                            aaTGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGc                                                    <  1:2754959/100‑1 (MQ=255)
                                            aaTGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGc                                                    >  1:5293042/1‑100 (MQ=255)
                                            aaTGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGc                                                    <  1:619160/100‑1 (MQ=255)
                                            aaTGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGc                                                    <  1:3293722/100‑1 (MQ=255)
                                               gtgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTgg                                                 >  1:2897052/1‑100 (MQ=255)
                                               gtgCAGGCCGCAGAGCGTATTGCATTGATTCCCAAACTGGTTGGCGTAGGCTTTGTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTgg                                                 >  2:5145026/1‑100 (MQ=255)
                                                tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTggn                                                <  1:1035352/100‑2 (MQ=255)
                                                tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTggg                                                >  1:2454272/1‑100 (MQ=255)
                                                tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTggg                                                >  1:3700054/1‑100 (MQ=255)
                                                 gCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGc                                               >  1:1088308/1‑100 (MQ=255)
                                                 gCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGc                                               <  2:2193839/100‑1 (MQ=255)
                                                  cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                              >  1:3535547/1‑100 (MQ=255)
                                                  cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                              <  1:446896/100‑1 (MQ=255)
                                                  cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                              >  1:1846223/1‑100 (MQ=255)
                                                  cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCg                                              >  1:3838736/1‑100 (MQ=255)
                                                   aGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGt                                             >  1:1254752/1‑100 (MQ=255)
                                                   aGGCCGCAGAGCGTATTGCACTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGt                                             >  1:4354668/1‑100 (MQ=255)
                                                    ggCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGtt                                            >  1:4120621/1‑100 (MQ=255)
                                                      ccGCAGGGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGa                                          <  2:3412243/100‑1 (MQ=255)
                                                      ccGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGa                                          >  2:3677677/1‑100 (MQ=255)
                                                      ccGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGa                                          <  2:4859445/100‑1 (MQ=255)
                                                      ccGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGa                                          <  2:2364881/100‑1 (MQ=255)
                                                       cGCAGAGGGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTGTGTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTTAAGAGCTGGGCGTTGAt                                         >  2:1796502/1‑100 (MQ=255)
                                                        gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                                        <  1:2212021/100‑1 (MQ=255)
                                                        gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                                        <  2:2284485/100‑1 (MQ=255)
                                                        gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg                                        <  2:2343283/100‑1 (MQ=255)
                                                          agagCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgtg                                      <  1:5512516/100‑1 (MQ=255)
                                                          agagCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgtg                                      >  1:430984/1‑100 (MQ=255)
                                                            agCGTATTGCATTTATTCCCATACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGAc                                    <  1:115190/100‑1 (MQ=255)
                                                            agCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGAc                                    <  2:3278080/100‑1 (MQ=255)
                                                            agCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGACAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGAc                                    >  2:3911261/1‑100 (MQ=255)
                                                             gCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGAcc                                   <  2:1767836/100‑1 (MQ=255)
                                                               gTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTa                                 <  2:3143969/100‑1 (MQ=255)
                                                               gTATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTa                                 <  1:1962895/100‑1 (MQ=255)
                                                                tATTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTac                                >  1:1598232/1‑100 (MQ=255)
                                                                 aTTGCATTTATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacg                               >  2:1990212/1‑100 (MQ=255)
                                                                      aTTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACggg                          <  1:4266960/100‑1 (MQ=255)
                                                                       tttATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGc                         >  2:2619337/1‑100 (MQ=255)
                                                                         tATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCg                       <  1:3711827/100‑1 (MQ=255)
                                                                         tATTCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCg                       >  2:4471916/1‑100 (MQ=255)
                                                                           ttCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGAc                     <  2:1307828/100‑1 (MQ=255)
                                                                           ttCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGAGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGAc                     >  2:5369723/1‑100 (MQ=255)
                                                                            tCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACa                    >  2:1124084/1‑100 (MQ=255)
                                                                            tCCCAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACa                    <  1:3245370/100‑1 (MQ=255)
                                                                             cccAAAGTGGTTGGCGTGGGCTTTTTTACCAGCGGTAGCAACGGCGCACGACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTCCGACGGGACGACAg                   >  2:38099/1‑100 (MQ=255)
                                                                             cccAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAg                   <  1:1786244/100‑1 (MQ=255)
                                                                              ccAAACTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGa                  >  1:4014097/1‑100 (MQ=255)
                                                                                 aaCTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAAcc               <  2:4253323/100‑1 (MQ=255)
                                                                                 aaCTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAAcc               <  1:2645083/100‑1 (MQ=255)
                                                                                  aCTGGTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAAccc              <  2:4918510/100‑1 (MQ=255)
                                                                                      gTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAgtg          <  2:546264/100‑1 (MQ=255)
                                                                                      gTTGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAgtg          >  1:3955255/1‑100 (MQ=255)
                                                                                        tGGCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGtt        <  1:4102170/100‑1 (MQ=255)
                                                                                          gCGTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTc      <  1:3481927/100‑1 (MQ=255)
                                                                                            gTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTg    <  1:4941331/100‑1 (MQ=255)
                                                                                            gTGGGCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTg    >  2:2013372/1‑100 (MQ=255)
                                                                                              gggCTTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGt  <  2:1882929/100‑1 (MQ=255)
                                                                                                 |                                                                                                
AAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGT  >  NC_000913/1605073‑1605266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A