Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,738,006 A→C K55Q (AAG→CAG)  purR → transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,738,0060AC98.5% 688.1 / ‑5.6 197K55Q (AAG→CAG) purRtranscriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (98/94);  total (100/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

CAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCC  >  NC_000913/1737909‑1738102
                                                                                                 |                                                                                                
caacaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAg                                                                                                <  2:3572997/100‑1 (MQ=255)
 aacaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAgg                                                                                               >  1:2043446/1‑100 (MQ=255)
  acaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGt                                                                                              >  2:4899438/1‑100 (MQ=255)
  acaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGt                                                                                              >  1:2931881/1‑100 (MQ=255)
  acaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGt                                                                                              >  2:3830947/1‑100 (MQ=255)
  acaaAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGt                                                                                              >  1:489349/1‑100 (MQ=255)
    aaaaCACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTa                                                                                            >  1:1908725/1‑100 (MQ=255)
     aaaCACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTaa                                                                                           >  1:5261690/1‑100 (MQ=255)
        cacGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAAccn                                                                                        <  2:1579989/100‑2 (MQ=255)
        cacGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACca                                                                                        >  1:1704909/1‑100 (MQ=255)
        cacGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACca                                                                                        >  2:1638868/1‑100 (MQ=255)
            tttCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACAcc                                                                                    <  2:3307859/100‑1 (MQ=255)
            tttCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACAcc                                                                                    <  1:2285420/100‑1 (MQ=255)
            tttCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACAcc                                                                                    <  1:2846019/100‑1 (MQ=255)
             ttCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCa                                                                                   <  2:4978483/100‑1 (MQ=255)
               cgtcgCTGAAGAAACGCGCAACGCCGTTTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCACGTAGCCTGCAGGTTAACCACACCAAg                                                                                 >  2:4236494/1‑100 (MQ=255)
               cgtcgCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAg                                                                                 <  1:5176744/100‑1 (MQ=255)
               cgtcgCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAg                                                                                 <  1:1052882/100‑1 (MQ=255)
                gtcgCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGt                                                                                <  2:2805238/100‑1 (MQ=255)
                 tcgCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTc                                                                               <  1:2209386/100‑1 (MQ=255)
                  cgCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCt                                                                              >  2:4809211/1‑100 (MQ=255)
                  agCTGAAGAAACGCGCAACGGCGTGTGGGCAGAGATTAAAGAATTACACTACTCTCACAGAGCGGTGGAGCGTCGCATGAAGGTTATCCAAACAAActct                                                                              >  2:5486838/2‑100 (MQ=255)
                   gCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTa                                                                             >  2:2153127/1‑100 (MQ=255)
                    cTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTAt                                                                            >  2:2980033/1‑100 (MQ=255)
                     tGAAGAATCGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATc                                                                           <  2:5108774/100‑1 (MQ=255)
                       aagaaACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCgg                                                                         <  1:4360015/100‑1 (MQ=255)
                        agaaACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTCCAGGTTAACCACACGAAGTCTATGGGt                                                                        >  2:5192197/1‑100 (MQ=255)
                         gaaACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGtt                                                                       >  1:4295432/1‑100 (MQ=255)
                           aaCGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTg                                                                     >  2:1286607/1‑100 (MQ=255)
                           aaCGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAACCTGCAGGTTAACCACACCAAGTCTATCGGTTTg                                                                     >  2:2875574/1‑100 (MQ=255)
                             cgcgCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCt                                                                   <  1:3904715/100‑1 (MQ=255)
                               cgcAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTgg                                                                 <  1:604821/100‑1 (MQ=255)
                                gcAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGc                                                                >  2:4308310/1‑100 (MQ=255)
                                   aCGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGAc                                                             <  1:4006311/100‑1 (MQ=255)
                                    cGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGAcc                                                            <  1:4877957/100‑1 (MQ=255)
                                     gCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACca                                                           >  1:563810/1‑100 (MQ=255)
                                      ccGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACcag                                                          >  1:2391424/1‑100 (MQ=255)
                                       cGTGTGGGCAGCGATTAAAGAATTAGACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCTGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACcagc                                                         <  2:2300443/100‑1 (MQ=255)
                                       cGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGagatcg                                                         >  2:570670/1‑95 (MQ=255)
                                       cGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACcagc                                                         >  2:986977/1‑100 (MQ=255)
                                          gtgGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCagcagc                                                      <  1:2170103/100‑1 (MQ=255)
                                            gggCAGCGATTAAAGAATTGCACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGa                                                    >  2:5037823/1‑100 (MQ=255)
                                            gggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCCAGTCTATCGGTTTGCTGGCGGCCAGCAGCGa                                                    >  1:4632149/1‑100 (MQ=255)
                                             ggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCGTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGaa                                                   >  2:5282021/1‑100 (MQ=255)
                                             ggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTCTCGGTTTGCTGGCGACCAGCcgcgca                                                   >  1:2742375/1‑98 (MQ=255)
                                             ggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGaa                                                   >  2:44317/1‑100 (MQ=255)
                                             ggCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGaa                                                   >  2:1238366/1‑100 (MQ=255)
                                              gCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAg                                                  >  1:771164/1‑100 (MQ=255)
                                                  cGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGc                                              <  2:3446327/100‑1 (MQ=255)
                                                   gATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGcc                                             >  1:3532389/1‑100 (MQ=255)
                                                    aTTAAAGAATTACACTTCTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCt                                            <  1:116790/100‑1 (MQ=255)
                                                       aaaGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTAtt                                         >  2:3351403/1‑100 (MQ=255)
                                                       aaaGAATTACACTACGCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTAtt                                         <  1:1549193/100‑1 (MQ=255)
                                                        aaGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTAttt                                        >  2:4355226/1‑100 (MQ=255)
                                                         aGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTAtttt                                       <  1:3970845/100‑1 (MQ=255)
                                                          gAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTg                                      <  1:3042088/100‑1 (MQ=255)
                                                          gAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTg                                      >  1:2346004/1‑100 (MQ=255)
                                                           aaTTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGc                                     <  2:3717783/100‑1 (MQ=255)
                                                           aaTTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGc                                     <  2:2471160/100‑1 (MQ=255)
                                                           aaTTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGc                                     <  1:5526972/100‑1 (MQ=255)
                                                            aTTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGcc                                    >  2:3147030/1‑100 (MQ=255)
                                                             ttACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCg                                   <  1:2692097/100‑1 (MQ=255)
                                                               acacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCgag                                 >  2:4563718/1‑100 (MQ=255)
                                                               acacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCgag                                 <  2:1327200/100‑1 (MQ=255)
                                                               acacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCgag                                 <  2:3287502/100‑1 (MQ=255)
                                                                cacTACTCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCgaga                                <  2:811370/100‑1 (MQ=255)
                                                                 actactCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGAt                               <  2:2198948/100‑1 (MQ=255)
                                                                  ctactCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATc                              <  2:3525475/100‑1 (MQ=255)
                                                                    actCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCAt                            <  2:5190031/100‑1 (MQ=255)
                                                                      tCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTg                          <  2:4619174/100‑1 (MQ=255)
                                                                      tCCCCTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTg                          <  2:4056251/100‑1 (MQ=255)
                                                                       ccccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGa                         >  2:1022885/1‑100 (MQ=255)
                                                                       ccccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGa                         >  1:60708/1‑100 (MQ=255)
                                                                        cccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGaa                        <  1:3422814/100‑1 (MQ=255)
                                                                        cccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCAGTGaa                        >  2:205616/1‑100 (MQ=255)
                                                                         ccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAg                       <  1:946259/100‑1 (MQ=255)
                                                                         ccTAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAg                       <  1:4968897/100‑1 (MQ=255)
                                                                           tAGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCa                     <  2:2904264/100‑1 (MQ=255)
                                                                            aGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAg                    >  2:4260743/1‑100 (MQ=255)
                                                                            aGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAg                    <  1:5354388/100‑1 (MQ=255)
                                                                            aGCGCGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAg                    <  2:2021573/100‑1 (MQ=255)
                                                                             gcgcGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGt                   <  1:3189895/100‑1 (MQ=255)
                                                                             gcgcGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGt                   <  1:4736163/100‑1 (MQ=255)
                                                                             gcgcGGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGt                   <  1:1387536/100‑1 (MQ=255)
                                                                              cgcgGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGtt                  >  1:2593276/1‑100 (MQ=255)
                                                                               gcgGTGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTg                 <  2:761220/100‑1 (MQ=255)
                                                                                   tGGCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaaa             <  1:5201125/100‑1 (MQ=255)
                                                                                    ggCGCGTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCCGTTGaaaaa            >  2:2424629/1‑100 (MQ=255)
                                                                                      cgcgTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaaaaaa          <  1:4885190/100‑1 (MQ=255)
                                                                                       gcgTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAAt         <  1:2552821/100‑1 (MQ=255)
                                                                                       gcgTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAAt         >  1:1592157/1‑100 (MQ=255)
                                                                                       gcgTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTCCCGAGATCATTGAAGCAGGTGAAAAAAAt         >  1:543294/1‑100 (MQ=255)
                                                                                         gTAGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTg       >  2:2811959/1‑100 (MQ=255)
                                                                                           aGCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCt     <  2:4175102/100‑1 (MQ=255)
                                                                                            gCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGTAGTTGAAAAAAATTGCtt    >  2:1784098/1‑100 (MQ=255)
                                                                                            gCCTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCtt    <  1:1833345/100‑1 (MQ=255)
                                                                                             ccTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTc   <  2:5414154/100‑1 (MQ=255)
                                                                                              cTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTcc  <  1:2504811/100‑1 (MQ=255)
                                                                                              cTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTcc  >  1:5509333/1‑100 (MQ=255)
                                                                                              cTGCAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTcc  <  1:1976279/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTAGCGCGGTGGCGCGTAGCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCC  >  NC_000913/1737909‑1738102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A