Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,155,954	G→A	G231D (GGC→GAC)	mdtB →	multidrug efflux system, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,155,954	0	G	A	98.6%	725.8 / ‑3.3	219	G231D (GGC→GAC)	mdtB	multidrug efflux system, subunit B
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (107/106); total (109/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

AACGCTCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAA  >  NC_000913/2155857‑2156049

aaCGCTCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGAcc                                                                                               <  1:2199546/100‑1 (MQ=255)

aaCGCTCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGAcc                                                                                               <  1:5491771/100‑1 (MQ=255)

 aCGCTCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGAccc                                                                                              >  2:1763423/1‑100 (MQ=255)

     tCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTcc                                                                                          <  2:3079861/100‑1 (MQ=255)

     tCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTcc                                                                                          <  2:4670836/100‑1 (MQ=255)

        ggCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGt                                                                                       >  1:3151395/1‑100 (MQ=255)

         gCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTg                                                                                      <  2:4989693/100‑1 (MQ=255)

         gCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTg                                                                                      <  1:2931898/100‑1 (MQ=255)

          cGATTGCCGCCCTCGGCCTGACCAGCGAAAGCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGc                                                                                     <  1:5304992/100‑1 (MQ=255)

          cGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGc                                                                                     <  2:3691746/100‑1 (MQ=255)

            aTTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCgg                                                                                   <  1:2434103/100‑1 (MQ=255)

               gccgccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCa                                                                                >  1:3735711/1‑100 (MQ=255)

               gccgccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCa                                                                                >  1:1331419/1‑100 (MQ=255)

                ccgccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAACAGGTAGCCTCGACGACCCTTCCCGTGCGGTCAc                                                                               >  1:2722425/1‑100 (MQ=255)

                 cgccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACg                                                                              >  2:4241927/1‑100 (MQ=255)

                 cgccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACg                                                                              <  2:3271498/100‑1 (MQ=255)

                  gccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGc                                                                             <  1:259895/100‑1 (MQ=255)

                  gccCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGc                                                                             <  1:116497/100‑1 (MQ=255)

                   cccTCGTCCTCACCAGCGAACCCGTGCTCACCGCCATTACCGGCGCCAACGTTAACTCGGAAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCt                                                                            >  2:4919519/1‑100 (MQ=255)

                      tCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTc                                                                         <  1:1123263/100‑1 (MQ=255)

                          ccTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCcgcg                                                                     <  1:287201/100‑1 (MQ=255)

                          ccTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCcgcg                                                                     <  1:663043/100‑1 (MQ=255)

                           cTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGa                                                                    >  1:2131469/1‑100 (MQ=255)

                            tGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGaa                                                                   >  2:3991591/1‑100 (MQ=255)

                             gACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAAc                                                                  <  2:4363544/100‑1 (MQ=255)

                             gACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAAc                                                                  >  1:4969862/1‑100 (MQ=255)

                              aCCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACg                                                                 >  1:2757942/1‑100 (MQ=255)

                              aCCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACg                                                                 <  1:2833050/100‑1 (MQ=255)

                              aCCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACg                                                                 <  1:3583480/100‑1 (MQ=255)

                               ccAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGa                                                                >  1:5076469/1‑100 (MQ=255)

                               ccAGCGAAACCGTGCCCACCGCCATTACCGACGCTAACGTTAACTAGGCAAAAGGTAGCCTCGACGACCCTTCCCGAGCGGTCACGCTTTACGCGAACGa                                                                >  1:4843259/1‑100 (MQ=255)

                                cAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGAc                                                               <  2:388984/100‑1 (MQ=255)

                                cAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGAc                                                               <  2:117720/100‑1 (MQ=255)

                                 tgCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGCCGACCCTTCAAGAGCGGTCCCGCTTTCCGCGCACGAcc                                                              >  2:105026/2‑100 (MQ=255)

                                 aGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGAcc                                                              >  2:5424183/1‑100 (MQ=255)

                                 aGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGAcc                                                              <  1:3967473/100‑1 (MQ=255)

                                 aGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGAcc                                                              <  1:5051357/100‑1 (MQ=255)

                                  gCGAAGCCGTGCGCACCGCCAGTACCGGCGCTGACTTTAACTGGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCa                                                             <  2:1153753/100‑1 (MQ=255)

                                    gAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGa                                                           >  1:766540/1‑100 (MQ=255)

                                    gAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGa                                                           <  2:48570/100‑1 (MQ=255)

                                     aaaCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGAt                                                          <  1:5257599/100‑1 (MQ=255)

                                      aaCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATg                                                         <  2:1567569/100‑1 (MQ=255)

                                       aCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGc                                                        >  1:2280599/1‑100 (MQ=255)

                                       aCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGc                                                        <  2:1984887/100‑1 (MQ=255)

                                       aCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGc                                                        >  2:1242350/1‑100 (MQ=255)

                                       aCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCGTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGc                                                        <  2:2913212/100‑1 (MQ=255)

                                       aCCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGAAGCCTCGACAACCCTTCCCGTGCGGTCACGCTTGCCGCGAACGACCAGATGc                                                        <  1:1896984/100‑1 (MQ=255)

                                        ccGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCa                                                       <  2:107047/100‑1 (MQ=255)

                                         cGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCaa                                                      >  2:5352134/1‑100 (MQ=255)

                                          gTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAAt                                                     >  1:3297706/1‑100 (MQ=255)

                                              gcACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATccgc                                                 >  1:4377437/1‑100 (MQ=255)

                                               cACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATccgcc                                                <  2:2731571/100‑1 (MQ=255)

                                                aCCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATccgccg                                               <  2:660021/100‑1 (MQ=255)

                                                   gCCATTACCGGCGCTTACGTTAACTCCGCAAAATGTAGCCTTGATGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCTCCGAag                                            <  2:5239141/100‑1 (MQ=255)

                                                   gCCATTACCGGCGCTAACGTTAACTCGGCAAAGGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAag                                            >  2:4519232/1‑100 (MQ=255)

                                                   gCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAag                                            <  2:1367598/100‑1 (MQ=255)

                                                    ccATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAaga                                           >  1:2532235/1‑100 (MQ=255)

                                                     cATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAagag                                          <  2:756652/100‑1 (MQ=255)

                                                     cATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAagag                                          >  2:3448349/1‑100 (MQ=255)

                                                      aTTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGt                                         >  1:560922/1‑100 (MQ=255)

                                                      aTTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGt                                         >  1:2637805/1‑100 (MQ=255)

                                                      aTTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGt                                         >  2:5080582/1‑100 (MQ=255)

                                                      aTTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGt                                         >  2:4025937/1‑100 (MQ=255)

                                                        tACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTAt                                       <  2:3900629/100‑1 (MQ=255)

                                                        tACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTAt                                       >  2:3501434/1‑100 (MQ=255)

                                                         aCCGGCGCTAACGTTAACGCGGCAAAAGGTAGCCTCGTGGTCCCTTCCCGTGCTGTCACGCTTTCCGCGAACGAGTAGATGCAATCCGCCGAAGTGTATc                                      <  2:1905031/100‑1 (MQ=255)

                                                          ccGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCg                                     >  2:63033/1‑100 (MQ=255)

                                                          ccGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCg                                     <  2:3603330/100‑1 (MQ=255)

                                                           cGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGc                                    >  2:1864571/1‑100 (MQ=255)

                                                            ggCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGcc                                   >  2:1406485/1‑100 (MQ=255)

                                                               gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGc                                >  2:4115253/1‑100 (MQ=255)

                                                               gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGc                                <  2:4292626/100‑1 (MQ=255)

                                                               gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGc                                >  2:4162501/1‑100 (MQ=255)

                                                                cTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCt                               >  1:1060658/1‑100 (MQ=255)

                                                                cTAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCGTTCCGCGAACGACCAGATGGAATCCGCCGAAGAGTATCGCCAGCt                               <  2:3813726/100‑1 (MQ=255)

                                                                 tAACGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTa                              >  1:2985236/1‑100 (MQ=255)

                                                                   aCGTTAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAat                            >  2:3666971/1‑100 (MQ=255)

                                                                    cGTTAACTCGGCAAAAGGCAGCGTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAatc                           >  1:4651707/1‑100 (MQ=255)

                                                                      ttAACTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAatcat                         >  2:2272854/1‑100 (MQ=255)

                                                                         aCTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGc                      >  1:3921926/1‑100 (MQ=255)

                                                                          cTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGcc                     <  1:3385663/100‑1 (MQ=255)

                                                                          cTCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGcc                     <  1:4502337/100‑1 (MQ=255)

                                                                           tCGGCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCt                    >  1:2403870/1‑100 (MQ=255)

                                                                              gCAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTAcc                 <  1:1259073/100‑1 (MQ=255)

                                                                               cAAAAGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCa                <  1:1762616/100‑1 (MQ=255)

                                                                                 aaaGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGa              >  2:1615119/1‑100 (MQ=255)

                                                                                 aaaGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGa              <  1:5497612/100‑1 (MQ=255)

                                                                                   aGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGaan            <  1:4434968/100‑2 (MQ=255)

                                                                                   aGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAAc            <  2:3178249/100‑1 (MQ=255)

                                                                                   aGGTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAAc            >  2:1948025/1‑100 (MQ=255)

                                                                                     gTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACgg          >  1:3773280/1‑100 (MQ=255)

                                                                                     gTAGCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACgg          <  1:1447787/100‑1 (MQ=255)

                                                                                        gCCTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgc       >  2:2592255/1‑100 (MQ=255)

                                                                                         ccTCGACGAGCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgcg      <  1:147694/100‑1 (MQ=255)

                                                                                         ccTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgcg      >  1:1626139/1‑100 (MQ=255)

                                                                                          cTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgcgc     >  1:4175174/1‑100 (MQ=255)

                                                                                          cTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgcgc     >  1:3843615/1‑100 (MQ=255)

                                                                                          cTCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgcgcgc     >  2:2713919/1‑100 (MQ=255)

                                                                                           tCGACGCCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc    <  2:900476/100‑1 (MQ=255)

                                                                                           tCGACGACCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc    <  1:2664901/100‑1 (MQ=255)

AACGCTCAGGCGATTGCCGCCCTCGGCCTGACCAGCGAAACCGTGCGCACCGCCATTACCGGCGCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAA  >  NC_000913/2155857‑2156049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A