Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,234,442 T→G L137W (TTG→TGG)  preT → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,234,4420TG97.9% 654.0 / 0.2 193L137W (TTG→TGG) preTdihydropyrimidine dehydrogenase, NADH‑dependent, subunit N
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (104/82);  total (109/84)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 193 total aligned reads displayed.

GTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGC  >  NC_000913/2234345‑2234537
                                                                                                 |                                                                                               
gTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGc                                                                                               <  2:123078/100‑1 (MQ=255)
  tACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAg                                                                                             >  2:946926/1‑100 (MQ=255)
   aCTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAgg                                                                                            >  2:197979/1‑100 (MQ=255)
   aCTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAgg                                                                                            >  2:3787375/1‑100 (MQ=255)
    cTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGc                                                                                           <  1:4369602/100‑1 (MQ=255)
    cTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGc                                                                                           >  1:4756585/1‑100 (MQ=255)
    cTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGc                                                                                           >  2:4917032/1‑100 (MQ=255)
     tGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGcc                                                                                          <  1:1845345/100‑1 (MQ=255)
     tGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGcc                                                                                          <  2:2108515/100‑1 (MQ=255)
      gATTTTGAACAACAATCTGGACTTGCAATTTCTCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCCCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  2:4535823/1‑100 (MQ=255)
      gATTTTGAACAACAAACCGGAATGGCAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  2:4377865/1‑100 (MQ=255)
      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  1:3325185/1‑100 (MQ=255)
      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  1:2236255/1‑100 (MQ=255)
      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  1:5022990/1‑100 (MQ=255)
      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCa                                                                                         >  2:885536/1‑100 (MQ=255)
        ttttGAACAACAAACCGGAATGGAAATTCATCAGCCCGGTACTAACACGCTCGGCAAAGTCGCACATATTGGCGCAGGTCCTGCCCGATGGCAGGCCAgt                                                                                       >  2:4248317/1‑100 (MQ=255)
         tttGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAgtg                                                                                      <  2:268871/100‑1 (MQ=255)
          ttGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAgtgt                                                                                     >  1:2625914/1‑100 (MQ=255)
             aacaacAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGac                                                                                  >  2:1891229/1‑100 (MQ=255)
             aacaacAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGac                                                                                  <  1:1236827/100‑1 (MQ=255)
             aacaacAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGac                                                                                  >  2:2768333/1‑100 (MQ=255)
             aacaacAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCGCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGac                                                                                  <  1:4705883/100‑1 (MQ=255)
               caacaaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGacac                                                                                >  1:4890638/1‑100 (MQ=255)
                aacaaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACt                                                                               <  1:2599984/100‑1 (MQ=255)
                 acaaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTg                                                                              >  1:970163/1‑100 (MQ=255)
                 acaaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTg                                                                              >  2:2863703/1‑100 (MQ=255)
                 acaaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTg                                                                              >  1:1197671/1‑100 (MQ=255)
                  caaACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGa                                                                             >  1:3853673/1‑100 (MQ=255)
                   aaaCCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGAc                                                                            <  2:5356638/100‑1 (MQ=255)
                   aaaCCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGAc                                                                            <  1:4194625/100‑1 (MQ=255)
                    aaCCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGGCGGATCGCAGGCCAGTGTGACACTGACa                                                                           >  1:5072074/1‑100 (MQ=255)
                    aaCCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACa                                                                           <  2:747933/100‑1 (MQ=255)
                    aaCCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACa                                                                           <  2:3034911/100‑1 (MQ=255)
                        ggAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAAcc                                                                       <  1:363860/100‑1 (MQ=255)
                        ggAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAAcc                                                                       <  2:3646287/100‑1 (MQ=255)
                          aaTGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAg                                                                     <  1:3688462/100‑1 (MQ=255)
                          aaTGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAg                                                                     <  2:1155027/100‑1 (MQ=255)
                           aTGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAgg                                                                    >  2:695258/1‑100 (MQ=255)
                            tGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAggg                                                                   >  1:3208743/1‑100 (MQ=255)
                            tGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAggg                                                                   <  2:325237/100‑1 (MQ=255)
                            tGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAggg                                                                   >  2:3184355/1‑100 (MQ=255)
                             ggAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGt                                                                  >  2:1381791/1‑100 (MQ=255)
                              gAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGtt                                                                 >  2:3440504/1‑100 (MQ=255)
                                 aTTTATCAGTCCGGTCCTACCACGCTCGGCAAGCTAGCGTTTATTGGCTCATGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATg                                                              >  2:1532013/1‑100 (MQ=255)
                                 aTTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATg                                                              <  2:2327170/100‑1 (MQ=255)
                                  tttATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGa                                                             >  2:1668272/1‑100 (MQ=255)
                                  tttATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGa                                                             <  2:3906361/100‑1 (MQ=255)
                                   ttATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGAc                                                            <  1:897383/100‑1 (MQ=255)
                                    tATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACg                                                           <  1:1160820/100‑1 (MQ=255)
                                      tCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTc                                                         <  2:799949/100‑1 (MQ=255)
                                        aGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCAc                                                       <  1:2175521/100‑1 (MQ=255)
                                         gCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACg                                                      >  2:2043700/1‑100 (MQ=255)
                                             ggTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGAttt                                                  >  2:2140292/1‑100 (MQ=255)
                                                cttAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATg                                               <  1:714340/98‑1 (MQ=255)
                                                aCTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATg                                               <  1:3145013/100‑1 (MQ=255)
                                                aCTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATg                                               >  2:3728626/1‑100 (MQ=255)
                                                aCTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATg                                               <  2:4049026/100‑1 (MQ=255)
                                                 cTAAAACGCTCGGCAAAGTCGCTATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGAGACTGACAAACCATGGTTATTACGTCGCGATTTAGga                                              >  2:2866824/1‑100 (MQ=255)
                                                 cTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATga                                              >  1:3539418/1‑100 (MQ=255)
                                                  tAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACGCTGACAAACCAGGGTTATGACGTCACGATTTATgag                                             >  1:5247037/1‑100 (MQ=255)
                                                   aaaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATgaga                                            >  1:2677441/1‑100 (MQ=255)
                                                   aaaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATgaga                                            >  1:1448375/1‑100 (MQ=255)
                                                   aaaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATgaga                                            >  1:133391/1‑100 (MQ=255)
                                                    aaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGCTTTATGagaa                                           >  2:3352797/1‑100 (MQ=255)
                                                       cGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGagaaaga                                        >  2:2565015/1‑100 (MQ=255)
                                                        gCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGagaaagaa                                       <  1:5520769/100‑1 (MQ=255)
                                                         cTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAaagaag                                      >  1:1824971/1‑100 (MQ=255)
                                                          tCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAgc                                     <  1:1912296/100‑1 (MQ=255)
                                                           cGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAgcg                                    >  1:622110/1‑100 (MQ=255)
                                                           cGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGATTATGACGTCACGATTTATGAGAAAGAAgcg                                    >  2:17716/1‑100 (MQ=255)
                                                            ggCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTCTGAGACACAAgcac                                   >  2:4146725/1‑98 (MQ=255)
                                                            ggCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAgcgc                                   <  1:5358124/100‑1 (MQ=255)
                                                             gCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCa                                  >  1:2994232/1‑100 (MQ=255)
                                                              cAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCAc                                 >  1:3988605/1‑100 (MQ=255)
                                                                   tCGCGATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCgg                            <  2:3868834/100‑1 (MQ=255)
                                                                    cgcgATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGt                           >  1:2780017/1‑100 (MQ=255)
                                                                     gcgATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTg                          <  1:3108630/100‑1 (MQ=255)
                                                                     gcgATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTg                          <  2:4035441/100‑1 (MQ=255)
                                                                     gcgATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTg                          >  2:4785235/1‑100 (MQ=255)
                                                                       gATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGc                        <  1:2453008/100‑1 (MQ=255)
                                                                       gATTATTGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGc                        <  1:4557293/100‑1 (MQ=255)
                                                                        nttattGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCt                       >  1:2106750/2‑100 (MQ=255)
                                                                         ttattGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTg                      <  2:5262204/100‑1 (MQ=255)
                                                                           attGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGt                    >  1:824454/1‑100 (MQ=255)
                                                                           attGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGt                    >  2:2181820/1‑100 (MQ=255)
                                                                             tGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGGCACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTg                  <  2:1293615/100‑1 (MQ=255)
                                                                             tGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTg                  <  1:2533939/100‑1 (MQ=255)
                                                                             tGGCGCAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTg                  <  1:781788/100‑1 (MQ=255)
                                                                                 gcAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTa              <  1:1742630/100‑1 (MQ=255)
                                                                                 gcAGGTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTa              <  1:1520510/100‑1 (MQ=255)
                                                                                    ggTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACg           >  1:2502398/1‑100 (MQ=255)
                                                                                    ggTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACg           >  1:2501986/1‑100 (MQ=255)
                                                                                     gTCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACgg          >  1:5156551/1‑100 (MQ=255)
                                                                                      tCCTGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGt         >  1:3754463/1‑100 (MQ=255)
                                                                                         tGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTAtt      >  1:3355875/1‑100 (MQ=255)
                                                                                         tGCCGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTAtt      >  1:3961958/1‑100 (MQ=255)
                                                                                            cGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCg   <  2:4692188/100‑1 (MQ=255)
                                                                                            cGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCg   <  1:578229/100‑1 (MQ=255)
                                                                                            cGGATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTAGGACGTCCCGATTTATGTGAAAGAAGCGTACCCCGGTGGCTGGTTGCGTAACGGTATTCCg   <  1:514032/100‑1 (MQ=255)
                                                                                             ggATGGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAATGGTATTTCGc  >  2:4365421/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGC  >  NC_000913/2234345‑2234537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A