Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,639	A→C	F413C (TTT→TGT)	rcsC ←	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,639	0	A	C	98.9%	640.3 / ‑1.9	179	F413C (TTT→TGT)	rcsC	hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (83/94); total (84/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 195 total aligned reads displayed.

ATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAA  >  NC_000913/2318542‑2318735

aTCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACacaa                                                                                                <  2:1686564/100‑1 (MQ=255)

   naccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTg                                                                                             >  1:5200371/2‑100 (MQ=255)

   caccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTg                                                                                             >  2:562149/1‑100 (MQ=255)

   caccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTg                                                                                             <  2:475584/100‑1 (MQ=255)

   caccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTg                                                                                             >  2:2198768/1‑100 (MQ=255)

   caccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTg                                                                                             <  1:2437201/100‑1 (MQ=255)

    accaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGa                                                                                            >  2:3386411/1‑100 (MQ=255)

     ccaGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGAc                                                                                           >  1:567478/1‑100 (MQ=255)

      caGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGAcc                                                                                          <  2:5050954/100‑1 (MQ=255)

           cacaAATGGGGAGGTTTTCATTACGATAGCGCGAATGTACGAAGCTGCTTTGCAGATTGGTATTGTTTCTGGTCAGGGCATCTACACAGTTGACctgctg                                                                                     >  2:5381680/1‑100 (MQ=255)

           cacaAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACctgctg                                                                                     >  2:3722192/1‑100 (MQ=255)

               aaTGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCca                                                                                 >  2:645217/1‑100 (MQ=255)

                aTGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCcac                                                                                <  2:3957954/100‑1 (MQ=255)

                 tGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCcaca                                                                               <  1:1011796/100‑1 (MQ=255)

                   gCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGa                                                                             <  1:4101580/100‑1 (MQ=255)

                   gCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGa                                                                             >  1:3642679/1‑100 (MQ=255)

                    ccACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGAt                                                                            >  1:3002503/1‑100 (MQ=255)

                     cACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATa                                                                           <  1:5256607/100‑1 (MQ=255)

                     cACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATa                                                                           >  1:3883753/1‑100 (MQ=255)

                        gTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAAtt                                                                        >  2:4679795/1‑100 (MQ=255)

                          tttCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTg                                                                      <  1:979554/100‑1 (MQ=255)

                          tttCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTg                                                                      <  2:2116032/100‑1 (MQ=255)

                           ttCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGc                                                                     <  1:4517866/100‑1 (MQ=255)

                            tCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTTCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCg                                                                    >  2:3093913/1‑100 (MQ=255)

                             cATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGt                                                                   >  2:1620234/1‑100 (MQ=255)

                             cATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGt                                                                   >  1:1632574/1‑100 (MQ=255)

                              nttACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTc                                                                  >  2:4653577/2‑100 (MQ=255)

                              aTTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTc                                                                  >  2:1184572/1‑100 (MQ=255)

                                tACGATAGCGCGATTGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTAGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAg                                                                <  1:5024636/100‑1 (MQ=255)

                                 aCGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGt                                                               <  2:1846920/100‑1 (MQ=255)

                                  cGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTc                                                              >  2:2883356/1‑100 (MQ=255)

                                   gATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCg                                                             >  1:347339/1‑100 (MQ=255)

                                    aTAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGt                                                            >  1:4238638/1‑100 (MQ=255)

                                       gcgcGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTgg                                                         >  1:3326838/1‑100 (MQ=255)

                                       gcgcGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTgg                                                         <  2:2190435/100‑1 (MQ=255)

                                        cgcgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGc                                                        <  1:625637/100‑1 (MQ=255)

                                        cgcgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGc                                                        >  2:1384777/1‑100 (MQ=255)

                                        cgcgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGc                                                        <  2:3533316/100‑1 (MQ=255)

                                        cgcgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGc                                                        >  2:3675540/1‑100 (MQ=255)

                                         gcgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCg                                                       <  2:2024838/100‑1 (MQ=255)

                                          cgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCgg                                                      >  2:3505948/1‑100 (MQ=255)

                                          cgAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCgg                                                      <  1:2231008/100‑1 (MQ=255)

                                           gAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGt                                                     <  1:3531224/100‑1 (MQ=255)

                                             aTGGACGAAGCTCATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTcc                                                   >  2:175915/1‑100 (MQ=255)

                                              tGGACGAAGCTGATTTGCAGATTGGTAATGTTGCCGGTCATTACGTCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCt                                                  <  2:5247353/100‑1 (MQ=255)

                                                    aaGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCg                                            <  2:4200045/100‑1 (MQ=255)

                                                     aGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGt                                           >  2:2450811/1‑100 (MQ=255)

                                                       cTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTaa                                         >  2:1959940/1‑100 (MQ=255)

                                                          aTTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCa                                      >  1:3985779/1‑100 (MQ=255)

                                                            ttGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCata                                    <  2:1135802/100‑1 (MQ=255)

                                                             tGCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCatat                                   <  1:4700614/100‑1 (MQ=255)

                                                              gCAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATAtt                                  >  2:2667138/1‑100 (MQ=255)

                                                               cAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTg                                 >  1:5005303/1‑100 (MQ=255)

                                                               cAGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTg                                 <  1:1778436/100‑1 (MQ=255)

                                                                aGATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTga                                <  1:2877754/100‑1 (MQ=255)

                                                                 gATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgag                               <  1:527400/100‑1 (MQ=255)

                                                                 gATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgag                               >  1:1192939/1‑100 (MQ=255)

                                                                 gATTGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTgag                               >  2:2362196/1‑100 (MQ=255)

                                                                   ttGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGAt                             >  2:3288506/1‑100 (MQ=255)

                                                                   ttGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGAt                             <  2:3144991/100‑1 (MQ=255)

                                                                    tGGTATTGTTGCTGGTCATGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGCCCTCATGCGTAAGCATATTGAGATa                            >  2:1018768/1‑100 (MQ=255)

                                                                    tGGTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATa                            >  2:4496214/1‑100 (MQ=255)

                                                                     ggTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAg                           >  1:1296758/1‑100 (MQ=255)

                                                                     ggTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAg                           >  2:481263/1‑100 (MQ=255)

                                                                     ggTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAg                           <  1:1396981/100‑1 (MQ=255)

                                                                      gTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAgn                          <  1:4434165/100‑2 (MQ=255)

                                                                      gTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAgg                          >  2:5162002/1‑100 (MQ=255)

                                                                      gTATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAgg                          <  1:1672128/100‑1 (MQ=255)

                                                                       tATTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGt                         <  2:1032800/100‑1 (MQ=255)

                                                                        aTTGTTGCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTa                        <  2:4935578/100‑1 (MQ=255)

                                                                         ttgttgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTAt                       <  1:4286958/100‑1 (MQ=255)

                                                                          tgttgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATg                      >  1:1616434/1‑100 (MQ=255)

                                                                          tgttgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATg                      <  1:4566677/100‑1 (MQ=255)

                                                                           gttgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATgc                     >  2:372777/1‑100 (MQ=255)

                                                                           gttgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATgc                     >  2:3914362/1‑100 (MQ=255)

                                                                             tgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATgcgc                   >  1:1596154/1‑100 (MQ=255)

                                                                             tgCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATgcgc                   >  1:2116564/1‑100 (MQ=255)

                                                                              gCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGcc                  <  1:4154469/100‑1 (MQ=255)

                                                                              gCTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGcc                  >  1:124107/1‑100 (MQ=255)

                                                                               cTGGTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCa                 >  2:2355118/1‑100 (MQ=255)

                                                                                 ggTCAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGt               >  2:2222081/1‑100 (MQ=255)

                                                                                    cAGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCg            >  1:3431152/1‑100 (MQ=255)

                                                                                     aGGACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGt           <  2:2005662/100‑1 (MQ=255)

                                                                                      ggACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGtt          <  1:722857/100‑1 (MQ=255)

                                                                                      ggACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGtt          <  2:3848223/100‑1 (MQ=255)

                                                                                      ggACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGtt          <  1:2221405/100‑1 (MQ=255)

                                                                                      ggACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGtt          <  1:237187/100‑1 (MQ=255)

                                                                                       gACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTa         <  2:728129/100‑1 (MQ=255)

                                                                                       gACATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTa         <  1:3783002/100‑1 (MQ=255)

                                                                                        aCATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTAc        <  2:1576683/100‑1 (MQ=255)

                                                                                         cATCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACt       >  1:3543256/1‑100 (MQ=255)

                                                                                          aTCAACACAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATGTTGAGATAGGTATGCGCCAGTTCGTTACtt      >  2:2960065/1‑100 (MQ=255)

                                                                                            caacacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaa    >  2:2312540/1‑100 (MQ=255)

                                                                                            caacacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaa    >  1:3627249/1‑100 (MQ=255)

                                                                                             aacacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaaa   <  1:788759/100‑1 (MQ=255)

                                                                                             aacacAATTGACCTGCTGCCCACACATAATTTGCGTCAGTCGTTGTCGGTCCTCATGCGTCAGCATATTGAGAAAGGTATGCGCCAGTTCGTTACTTaac   >  2:4542733/1‑99 (MQ=255)

                                                                                              acacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaaca  >  1:5408254/1‑98 (MQ=255)

                                                                                              acacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaaaa  >  2:1637931/1‑100 (MQ=255)

                                                                                              acacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaaaa  >  2:123163/1‑100 (MQ=255)

                                                                                              acacAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTaaaa  >  1:1034509/1‑100 (MQ=255)

ATCCACCAGCACACAAATGGCCACGTTTTCATTACGATAGCGCGAATGGACGAAGCTGATTTGCAGATTGGTATTGTTGCTGGTCAGGACATCAACAAAATTGACCTGCTGCCCACAGATAATTTGCGTCAGTCGTTGGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAA  >  NC_000913/2318542‑2318735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A