Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,488,822 A→C L116R (CTT→CGT)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,488,8220AC98.5% 683.7 / ‑1.1 194L116R (CTT→CGT) yfdEacetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (96/95);  total (98/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 196 total aligned reads displayed.

TGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTT  >  NC_000913/2488726‑2488918
                                                                                                |                                                                                                
tGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAcgcg                                                                                               >  2:4188082/1‑100 (MQ=255)
   atgatgGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGttt                                                                                            >  1:2954284/1‑100 (MQ=255)
    tgatgGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTc                                                                                           <  2:1600960/100‑1 (MQ=255)
     gatgGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTcc                                                                                          >  1:1049756/1‑100 (MQ=255)
        ggTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCAt                                                                                       <  2:3797647/100‑1 (MQ=255)
         gTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATg                                                                                      >  2:4751957/1‑100 (MQ=255)
          tATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGa                                                                                     <  1:1865508/100‑1 (MQ=255)
           aTCGTAGGCAGGAGCATCTTTTTTCTTATTTGTATGTCCGAAACACGACTATGAAGCATATATGAAGCGGGGGTTGGGTTGTTGGCGCGTTTCCCATGaa                                                                                    >  2:2147362/1‑100 (MQ=255)
           aTCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGaa                                                                                    >  2:1378018/1‑100 (MQ=255)
           aTCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGaa                                                                                    >  2:1207850/1‑100 (MQ=255)
                 ggCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAAcccc                                                                              >  1:3745115/1‑100 (MQ=255)
                 ggCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAAcccc                                                                              >  2:3369084/1‑100 (MQ=255)
                   cAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAg                                                                            >  2:1690943/1‑100 (MQ=255)
                   cAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAg                                                                            <  2:3990664/100‑1 (MQ=255)
                     ggAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtt                                                                          >  2:2112775/1‑100 (MQ=255)
                      gAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGttt                                                                         <  2:4683204/100‑1 (MQ=255)
                       aGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGtttt                                                                        >  1:5498867/1‑100 (MQ=255)
                        gCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGttttt                                                                       >  2:3086560/1‑100 (MQ=255)
                        gCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGttttt                                                                       <  1:2931075/100‑1 (MQ=255)
                        gCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTAGATTTCTTGACGCGTTTCCCATGAAAACCCCAGttttt                                                                       >  1:5320483/1‑100 (MQ=255)
                         cATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTc                                                                      <  1:3397461/100‑1 (MQ=255)
                         cATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTc                                                                      <  2:4407336/100‑1 (MQ=255)
                          aTCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTcc                                                                     <  2:2012842/100‑1 (MQ=255)
                          aTCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTcc                                                                     <  1:2975364/100‑1 (MQ=255)
                          aTCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTcc                                                                     <  1:217846/100‑1 (MQ=255)
                          aTCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTcc                                                                     >  2:3299345/1‑100 (MQ=255)
                           tCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCa                                                                    <  1:2578438/100‑1 (MQ=255)
                           tCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCa                                                                    <  1:4292857/100‑1 (MQ=255)
                            cTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAt                                                                   >  2:5308825/1‑100 (MQ=255)
                             ttttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAtt                                                                  <  1:1902047/100‑1 (MQ=255)
                             ttttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAtt                                                                  >  2:2615413/1‑100 (MQ=255)
                             ttttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAtt                                                                  >  1:842491/1‑100 (MQ=255)
                              tttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTg                                                                 >  1:2067517/1‑100 (MQ=255)
                               ttAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGt                                                                <  2:73952/100‑1 (MQ=255)
                                tAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATGTATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTa                                                               <  2:4033507/100‑1 (MQ=255)
                                tAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTa                                                               >  2:1640622/1‑100 (MQ=255)
                                  gCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTAcc                                                             <  1:4409620/100‑1 (MQ=255)
                                    ggACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTg                                                           >  2:3939182/1‑100 (MQ=255)
                                     gACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTgg                                                          <  1:2135181/100‑1 (MQ=255)
                                       ccGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGc                                                        >  2:2315985/1‑100 (MQ=255)
                                       ccGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGc                                                        >  2:1153378/1‑100 (MQ=255)
                                        cGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCg                                                       <  1:1709742/100‑1 (MQ=255)
                                         ggTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGa                                                      >  2:3184364/1‑100 (MQ=255)
                                           tATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGaaa                                                    >  1:3091356/1‑100 (MQ=255)
                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGTGTTTCCCATGTAATCCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                  <  1:5378843/100‑1 (MQ=255)
                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                  >  1:509200/1‑100 (MQ=255)
                                             tGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAt                                                  <  1:765189/100‑1 (MQ=255)
                                              gTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAtt                                                 >  1:1625567/1‑100 (MQ=255)
                                              gTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAAtt                                                 >  1:1374617/1‑100 (MQ=255)
                                                ccGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtct                                               >  1:110082/1‑100 (MQ=255)
                                                ccGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtct                                               >  1:1223780/1‑100 (MQ=255)
                                                ccGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtct                                               <  1:4929597/100‑1 (MQ=255)
                                                 cGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAGAACGCCAGTTTTTCCATTGTACCTGGGCGAAAATtctc                                              <  2:2133335/100‑1 (MQ=255)
                                                 cGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCGTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATtctc                                              <  2:4945490/100‑1 (MQ=255)
                                                  gAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCa                                             >  2:770705/1‑100 (MQ=255)
                                                   aaaCCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAg                                            <  1:4704518/100‑1 (MQ=255)
                                                       cTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTaa                                        >  1:3302385/1‑100 (MQ=255)
                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                       >  2:921976/1‑100 (MQ=255)
                                                        tGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                       <  2:5071567/100‑1 (MQ=255)
                                                        tGACGATGAAGCAGATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAAt                                       <  2:4201685/100‑1 (MQ=255)
                                                         gACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATa                                      <  2:970199/100‑1 (MQ=255)
                                                          aCGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATAc                                     <  1:5508265/100‑1 (MQ=255)
                                                           cGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACa                                    >  1:334566/1‑100 (MQ=255)
                                                             aTGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATc                                  <  1:276119/100‑1 (MQ=255)
                                                              tGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCa                                 <  2:3672129/100‑1 (MQ=255)
                                                              tGAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCa                                 <  1:3940239/100‑1 (MQ=255)
                                                               gAAGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAg                                >  2:4800872/1‑100 (MQ=255)
                                                               gAAGCAGATATCAAGCGCAGGCAGATTTCATGACGCGTTGCCCATGAAAATCCCAATTCTTCCATTGTACCTGGGCGAAATTTCTCCGCGAATACATcgg                                <  2:860655/100‑3 (MQ=255)
                                                                aaGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGATTAACCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGc                               >  2:5247388/1‑100 (MQ=255)
                                                                 aGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCt                              >  2:3449984/1‑100 (MQ=255)
                                                                 aGCATATATGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCt                              <  1:4869543/100‑1 (MQ=255)
                                                                    atatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGt                           >  2:3033293/1‑100 (MQ=255)
                                                                    atatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGt                           <  1:2695368/100‑1 (MQ=255)
                                                                    atatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGt                           <  1:2557393/100‑1 (MQ=255)
                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                          <  2:3191828/100‑1 (MQ=255)
                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                          <  2:4887304/100‑1 (MQ=255)
                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                          >  1:2830132/1‑100 (MQ=255)
                                                                     tatatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGtt                          <  2:1763366/100‑1 (MQ=255)
                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCCGTTTTTCCATTGTACCTGTGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                        <  1:5177673/100‑1 (MQ=255)
                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                        >  2:4805801/1‑100 (MQ=255)
                                                                       tatGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTg                        <  2:155169/100‑1 (MQ=255)
                                                                        atGAGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGa                       <  1:4392635/100‑1 (MQ=255)
                                                                          gAGCCGCGTGTTTACTTCTTGACCCTTTTCCCATGAAAACCCCTTTTTTTCCATTTTACATGGGCGAAAATTCTCAGCTAAAACATCAGCTTGTTTGAGc                     <  2:4736553/100‑1 (MQ=255)
                                                                           aGGCGCGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCa                    <  1:5023623/100‑1 (MQ=255)
                                                                            ngcgcGGGTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCat                   >  1:552835/2‑100 (MQ=255)
                                                                                 gggTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTta              >  1:5456801/1‑100 (MQ=255)
                                                                                 gggTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTta              <  1:3482320/100‑1 (MQ=255)
                                                                                   gTTGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTtata            <  1:5156586/100‑1 (MQ=255)
                                                                                    ttGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATaa           >  1:4184165/1‑100 (MQ=255)
                                                                                    ttGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATaa           >  1:1448726/1‑100 (MQ=255)
                                                                                     tGATTTCTTGACGCGTTTCCCATGAAAACCCCATTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATaaa          <  2:1468539/100‑1 (MQ=255)
                                                                                     tGATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATaaa          >  1:1529589/1‑100 (MQ=255)
                                                                                     cgATTTCTTGACGGGTTTCCCATGAAAACCCCAGTTTTTCCATTGCACCTGGTCGAAAATTCTCAGCTAATACGTCAGCTTGTTTCAGCATATTTATaaa          <  2:5127404/99‑1 (MQ=255)
                                                                                      gATTTCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAat         >  1:69487/1‑100 (MQ=255)
                                                                                        tttCTTGACGCAGTTCCCATAAAAACCCCAATTTTTCCATTGTACCTAGGCGAAAATTCTCAGCTAACACATCCGCTTGTTTGCCACTATTTATAAttat       <  2:2759431/100‑1 (MQ=255)
                                                                                          tCTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATAc     <  2:1823907/100‑1 (MQ=255)
                                                                                           cTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATAcn    <  1:4448111/100‑2 (MQ=255)
                                                                                           cTTGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACt    <  1:1556843/100‑1 (MQ=255)
                                                                                             tGACGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATAAttt  >  2:2535723/1‑100 (MQ=255)
                                                                                             tGACGCGTTTCCCATGAAAACCCCAGTTTTTCCAGTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACttt  <  2:471407/100‑1 (MQ=255)
                                                                                                |                                                                                                
TGAATGATGGTATCGTAGGCAGGAGCATCTTTTAGCGGACCGGTATGTCCGAAACCTGACGATGAAGCATATATGAGGCGCGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTT  >  NC_000913/2488726‑2488918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A