Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,698,512 T→C L13L (TTA→TTG)  pgpC ← phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,698,5120TC97.4% 654.7 / 0.4 197L13L (TTA→TTG) pgpCphosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (105/81);  total (107/90)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACA  >  NC_000913/2698416‑2698608
                                                                                                |                                                                                                
 aacaacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGt                                                                                              >  1:2855579/1‑100 (MQ=255)
  acaacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCACTGTTCCATCCAAGTc                                                                                             >  2:1722908/1‑100 (MQ=255)
  acaacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTc                                                                                             >  2:2605467/1‑100 (MQ=255)
  acaacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTc                                                                                             <  2:2798554/100‑1 (MQ=255)
  acaacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTc                                                                                             >  2:4530217/1‑100 (MQ=255)
   caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCa                                                                                            >  1:363818/1‑100 (MQ=255)
   caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCa                                                                                            >  2:3093812/1‑100 (MQ=255)
   caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCa                                                                                            >  1:175850/1‑100 (MQ=255)
   caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCa                                                                                            >  2:4707431/1‑100 (MQ=255)
   caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGAAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCa                                                                                            >  2:4979647/1‑100 (MQ=255)
     acGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCaaa                                                                                          <  1:3967050/100‑1 (MQ=255)
          aGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAaca                                                                                     <  2:4167937/100‑1 (MQ=255)
           ggACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAacac                                                                                    <  2:5360424/100‑1 (MQ=255)
           ggACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAacac                                                                                    >  1:3273826/1‑100 (MQ=255)
           ctaCAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAacac                                                                                    <  1:563768/98‑1 (MQ=255)
            gACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAAcacc                                                                                   <  2:1995054/100‑1 (MQ=255)
            gACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAAcacc                                                                                   <  1:162441/100‑1 (MQ=255)
             aCAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAacacag                                                                                  >  2:557846/1‑98 (MQ=255)
              cAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAAcaccac                                                                                 >  1:4544584/1‑100 (MQ=255)
               gactAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACcaca                                                                                <  2:4655016/97‑1 (MQ=255)
                   aaCGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACacgac                                                                            <  2:1187401/100‑1 (MQ=255)
                     cGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGc                                                                          >  1:2753170/1‑100 (MQ=255)
                        aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCg                                                                       >  1:4646569/1‑100 (MQ=255)
                        aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCg                                                                       <  1:378675/100‑1 (MQ=255)
                        aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCg                                                                       <  1:3088732/100‑1 (MQ=255)
                         ttCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGt                                                                      >  1:2289583/1‑100 (MQ=255)
                          tCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTg                                                                     <  1:4505252/100‑1 (MQ=255)
                           cAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGa                                                                    >  2:3893179/1‑100 (MQ=255)
                           cAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGa                                                                    >  1:3827081/1‑100 (MQ=255)
                            aGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAg                                                                   <  1:5497022/100‑1 (MQ=255)
                             gCGGTTGGCGACGTAGTAAATAGCGCATAAAAATGGATAACATATCCAGCTGATGCAATGTTCCATAAAAGAAAAAAAACACCACACGACGCTCGTGAGt                                                                  <  2:3420727/100‑1 (MQ=255)
                             gCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGt                                                                  >  1:5083607/1‑100 (MQ=255)
                              cGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGtt                                                                 <  2:3606718/100‑1 (MQ=255)
                               ggTTGGCGGCCTAGGAAATAGCGCAGAAAACTTCCGAACATATCCTGCTGATTCAATGTTAAAACAAAGAAAAAAAACACCACACGACGCTCGTGAGTTg                                                                <  2:4892228/100‑1 (MQ=255)
                               ggTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTg                                                                <  1:2088092/100‑1 (MQ=255)
                               ggTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTg                                                                >  1:3701340/1‑100 (MQ=255)
                                  tGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCa                                                             >  1:4676554/1‑100 (MQ=255)
                                     cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAAc                                                          >  1:5446114/1‑100 (MQ=255)
                                     cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAAc                                                          >  1:4123978/1‑100 (MQ=255)
                                     cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAAc                                                          >  1:2622383/1‑100 (MQ=255)
                                     cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAAc                                                          >  1:1048364/1‑100 (MQ=255)
                                       aCGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCa                                                        <  1:143991/100‑1 (MQ=255)
                                        cGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCAt                                                       <  2:2439010/100‑1 (MQ=255)
                                         gtagtaAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATa                                                      >  1:2837470/1‑100 (MQ=255)
                                           agtaAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCGATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATAct                                                    >  1:1388504/1‑100 (MQ=255)
                                           agtaAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATAct                                                    >  1:1706822/1‑100 (MQ=255)
                                             taAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATActcc                                                  <  1:2310947/100‑1 (MQ=255)
                                             taAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATActcc                                                  <  1:1175402/100‑1 (MQ=255)
                                             taAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGAAGCTCGTGAGTTGCCAAACCATActcc                                                  >  1:3334425/1‑100 (MQ=255)
                                              aaaTAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATActcct                                                 >  2:98055/1‑100 (MQ=255)
                                                aTAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCctct                                               >  1:2659974/1‑100 (MQ=255)
                                                 tAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTg                                              <  1:1122495/100‑1 (MQ=255)
                                                 tAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTg                                              <  1:914475/100‑1 (MQ=255)
                                                 tAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTg                                              <  2:4229916/100‑1 (MQ=255)
                                                    cgcAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGAc                                           <  1:3474104/100‑1 (MQ=255)
                                                     gcAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACt                                          >  1:4814132/1‑100 (MQ=255)
                                                     gcAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACt                                          >  2:1956503/1‑100 (MQ=255)
                                                        gAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGaa                                       >  1:193762/1‑100 (MQ=255)
                                                        gAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGaa                                       <  2:4880435/100‑1 (MQ=255)
                                                         aaaaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGaaa                                      <  2:415586/100‑1 (MQ=255)
                                                         aaaaCTCGCGAACATCTCCTGCTGATGCAACGTTCCATCCACGTCTAAAATCACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGTCAGaaa                                      <  2:5176538/100‑1 (MQ=255)
                                                          aaaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAAc                                     <  1:5049658/100‑1 (MQ=255)
                                                          aaaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAAc                                     >  1:43857/1‑100 (MQ=255)
                                                           aaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACa                                    >  2:2465273/1‑100 (MQ=255)
                                                             cTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATc                                  >  1:293771/1‑100 (MQ=255)
                                                             cTGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATc                                  >  1:2821432/1‑100 (MQ=255)
                                                              tGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATcc                                 >  2:800963/1‑100 (MQ=255)
                                                              tGCCGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATcc                                 <  1:258887/100‑1 (MQ=255)
                                                                ccGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTg                               <  1:2398792/100‑1 (MQ=255)
                                                                ccGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTg                               <  2:1303220/100‑1 (MQ=255)
                                                                 cGAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGa                              >  2:4432225/1‑100 (MQ=255)
                                                                  gAACATATCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGAt                             <  2:5043110/100‑1 (MQ=255)
                                                                       tatCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAtt                        >  1:5277164/1‑100 (MQ=255)
                                                                         tCCTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAtttt                      >  1:1153349/1‑100 (MQ=255)
                                                                         nccTGCTGATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAtttt                      >  1:357649/2‑100 (MQ=255)
                                                                           ctgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCg                    >  1:2698007/1‑100 (MQ=255)
                                                                           ctgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCg                    <  2:5205363/100‑1 (MQ=255)
                                                                           ctgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCg                    <  1:946038/100‑1 (MQ=255)
                                                                           ctgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCg                    <  2:4073633/100‑1 (MQ=255)
                                                                           ctgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCg                    <  2:326265/100‑1 (MQ=255)
                                                                            tgctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGc                   <  2:1043629/100‑1 (MQ=255)
                                                                             gctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGct                  <  1:2172474/100‑1 (MQ=255)
                                                                              ctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGcta                 <  1:1480326/100‑1 (MQ=255)
                                                                              ctgATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGcta                 <  1:3991701/100‑1 (MQ=255)
                                                                                gATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctact               <  2:2266184/100‑1 (MQ=255)
                                                                                gATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctact               >  2:5884/1‑100 (MQ=255)
                                                                                gATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctact               >  1:4031411/1‑100 (MQ=255)
                                                                                gATGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctact               >  2:5294722/1‑100 (MQ=255)
                                                                                 aTGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctacta              <  2:1138005/100‑1 (MQ=255)
                                                                                  tGCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCtactac             <  2:3667272/100‑1 (MQ=255)
                                                                                   gCAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACa            >  2:2208781/1‑100 (MQ=255)
                                                                                    cAATGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACAt           >  2:1261393/1‑100 (MQ=255)
                                                                                     aaTGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATa          <  1:4761641/100‑1 (MQ=255)
                                                                                     aaTGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATa          <  2:3842323/100‑1 (MQ=255)
                                                                                      aTGTTCCATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAg         <  2:1481947/100‑1 (MQ=255)
                                                                                            cATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAAc   <  2:2593003/100‑1 (MQ=255)
                                                                                            cATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAAc   >  1:4702030/1‑100 (MQ=255)
                                                                                            cATCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAAc   >  2:3767502/1‑100 (MQ=255)
                                                                                             aTCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACa  >  1:3589003/1‑100 (MQ=255)
                                                                                             aTCCAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACa  >  2:5468543/1‑100 (MQ=255)
                                                                                                |                                                                                                
TAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACA  >  NC_000913/2698416‑2698608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A