Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,061,235 A→C Y24S (TAT→TCT)  yihM → putative sugar phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,061,2350AC97.6% 715.5 / 2.8 207Y24S (TAT→TCT) yihMputative sugar phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (95/107);  total (97/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 207 total aligned reads displayed.

ACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGT  >  NC_000913/4061138‑4061331
                                                                                                 |                                                                                                
aCCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTg                                                                                                <  1:3883664/100‑1 (MQ=255)
  cTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAAAAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGcc                                                                                              >  1:4861570/1‑100 (MQ=255)
     atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCttt                                                                                           <  1:4598292/100‑1 (MQ=255)
          tAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCAttt                                                                                      <  1:3276687/100‑1 (MQ=255)
           aaTCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTa                                                                                     <  1:568747/100‑1 (MQ=255)
           aaTCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTa                                                                                     <  2:4649438/100‑1 (MQ=255)
           aaTCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTa                                                                                     >  1:2076208/1‑100 (MQ=255)
             tCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTaaa                                                                                   <  2:4427250/100‑1 (MQ=255)
             tCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTaaa                                                                                   <  1:928000/100‑1 (MQ=255)
             tCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTaaa                                                                                   <  2:1069061/100‑1 (MQ=255)
              gtGTACGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAat                                                                                  <  2:5122174/99‑1 (MQ=255)
              cTGTATGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACAGGCTTCTGCCTTTCATTTAAat                                                                                  <  2:822894/100‑1 (MQ=255)
              cTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAat                                                                                  >  2:3288720/1‑100 (MQ=255)
                 tAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATg                                                                               <  1:5246799/100‑1 (MQ=255)
                    gtgtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCgg                                                                            <  1:4236481/100‑1 (MQ=255)
                     tgtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGc                                                                           <  1:3499380/100‑1 (MQ=255)
                      gtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGca                                                                          >  2:3466916/1‑99 (MQ=255)
                      gtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCt                                                                          >  1:3349961/1‑100 (MQ=255)
                      gtTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCt                                                                          >  2:210760/1‑100 (MQ=255)
                          tATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGaa                                                                      >  2:421159/1‑100 (MQ=255)
                           aTGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGaaa                                                                     <  1:492977/100‑1 (MQ=255)
                           aTGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGaaa                                                                     <  1:1114303/100‑1 (MQ=255)
                              gTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAagag                                                                  >  1:207035/1‑100 (MQ=255)
                              gTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAagag                                                                  >  2:51951/1‑100 (MQ=255)
                              gTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGAAACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAagag                                                                  <  2:2213699/100‑1 (MQ=255)
                                gACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAgtg                                                                >  1:2600313/1‑100 (MQ=255)
                                gACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAgtg                                                                <  2:4225087/100‑1 (MQ=255)
                                  cAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGtt                                                              >  2:1207091/1‑100 (MQ=255)
                                  cAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGtt                                                              >  1:1137929/1‑100 (MQ=255)
                                   aataaataACGCAAGAAAGATTCTACAACGGGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTg                                                             <  1:4311466/100‑1 (MQ=255)
                                    ataaataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGc                                                            >  1:1661137/1‑100 (MQ=255)
                                    ataaataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGc                                                            >  1:2364616/1‑100 (MQ=255)
                                     taaataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGcc                                                           <  1:173798/100‑1 (MQ=255)
                                      aaataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCct                                                          >  2:1402563/1‑100 (MQ=255)
                                        ataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgc                                                        <  2:341444/100‑1 (MQ=255)
                                        ataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgc                                                        <  1:1021995/100‑1 (MQ=255)
                                        ataaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgc                                                        <  1:776621/100‑1 (MQ=255)
                                         taaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgct                                                       <  2:2843530/100‑1 (MQ=255)
                                         taaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgct                                                       <  2:3116040/100‑1 (MQ=255)
                                          aaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgctn                                                      <  1:4450434/100‑2 (MQ=255)
                                          aaCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctgctg                                                      >  2:1607027/1‑100 (MQ=255)
                                           aCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGa                                                     <  1:3783686/100‑1 (MQ=255)
                                            cGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt                                                    <  2:4571503/100‑1 (MQ=255)
                                            cGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt                                                    <  2:3678346/100‑1 (MQ=255)
                                             gCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAtt                                                   >  2:3501070/1‑100 (MQ=255)
                                             gCAAGAAAGATTCCACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAtt                                                   >  2:1752701/1‑100 (MQ=255)
                                              cAAGAAAGATTCTTCAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAttt                                                  <  1:5015659/100‑1 (MQ=255)
                                              cAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAttt                                                  >  2:440200/1‑100 (MQ=255)
                                              cAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAttt                                                  >  2:3874544/1‑100 (MQ=255)
                                                agaaagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTAc                                                >  1:2047507/1‑100 (MQ=255)
                                                  aaagaTTCTACAACGTGTCGACCCTCTTCCTATTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGCGTGTTCCCCGCTGATTTACtt                                              >  2:3605997/1‑100 (MQ=255)
                                                  aaagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACtt                                              <  1:843212/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTg                                             <  1:1823651/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTg                                             <  1:1202543/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTg                                             <  2:1583646/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTg                                             >  1:5111931/1‑100 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTg                                             <  2:3005430/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTCACTTg                                             <  2:4894731/100‑1 (MQ=255)
                                                   aagaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTATTGCCTGCTGATTTACTTg                                             <  1:1263081/100‑1 (MQ=255)
                                                     gaTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGat                                           <  2:526332/100‑1 (MQ=255)
                                                      aTTCTACACCCTGTCAACACTATTCCTCATTATTTACATGCTTCGGCCTATCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTAGata                                          <  2:3441156/100‑1 (MQ=255)
                                                      aTTCTACAACGTGTCGACACTCTTCCTCTTTATTTAGATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGata                                          <  2:2398337/100‑1 (MQ=255)
                                                      aTTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGata                                          >  2:2278064/1‑100 (MQ=255)
                                                        tCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATc                                        >  1:2145229/1‑100 (MQ=255)
                                                         cTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCg                                       <  2:2965790/100‑1 (MQ=255)
                                                          tACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGc                                      <  2:4993445/100‑1 (MQ=255)
                                                           aCAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCa                                     <  1:651919/100‑1 (MQ=255)
                                                           aCAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCa                                     >  2:1286646/1‑100 (MQ=255)
                                                           aCAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCa                                     >  2:1555492/1‑100 (MQ=255)
                                                            cAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCaa                                    >  2:3550983/1‑100 (MQ=255)
                                                              aCGTGTCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGt                                  >  2:3623198/1‑100 (MQ=255)
                                                                gtgtCGACACTCTTCCTCTTTATTTACATGCTGCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGa                                <  2:5498604/100‑1 (MQ=255)
                                                                 tgtCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaa                               <  1:1475852/100‑1 (MQ=255)
                                                                 tgtCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaa                               <  2:2592053/100‑1 (MQ=255)
                                                                   tCGACACTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGaaaa                             <  1:864023/100‑1 (MQ=255)
                                                                    cGACACGCTTCCTCTTTATTTACATGCTTCTGCCTTGCATTTAAATATGCTGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAaat                            <  1:307097/100‑1 (MQ=255)
                                                                      acacTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAaataa                          >  2:3992562/1‑100 (MQ=255)
                                                                       cacTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAaataat                         <  2:2668040/100‑1 (MQ=255)
                                                                        acTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATc                        <  1:2258504/100‑1 (MQ=255)
                                                                        acTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATc                        <  2:4166636/100‑1 (MQ=255)
                                                                        acTCTTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCCGCTGATTTACTTGATATCGCAAGTGAAAATAATc                        <  1:3017652/100‑1 (MQ=255)
                                                                         ctctTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCt                       >  1:5388617/1‑100 (MQ=255)
                                                                          tctTCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTa                      <  1:2452251/100‑1 (MQ=255)
                                                                            ttCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGAGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACg                    <  2:777902/100‑1 (MQ=255)
                                                                             tCCTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGt                   >  1:4379474/1‑100 (MQ=255)
                                                                              ccTCTTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTg                  >  2:3714126/1‑100 (MQ=255)
                                                                                 ctTTATTTACATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCg               <  2:885260/100‑1 (MQ=255)
                                                                                  tttatttaCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTCCTTGATATCGCAAGTGAAAATAATCTACGTGGCGt              >  2:4637178/1‑100 (MQ=255)
                                                                                  tttatttaCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGt              >  1:4204779/1‑100 (MQ=255)
                                                                                   ttatttaCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTc             <  1:1574877/100‑1 (MQ=255)
                                                                                   ttatttaCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTc             >  1:3449884/1‑100 (MQ=255)
                                                                                      tttaCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCaaa          >  1:4071130/1‑100 (MQ=255)
                                                                                        taCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAAt        >  1:2723059/1‑100 (MQ=255)
                                                                                        taCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAAt        <  2:767663/100‑1 (MQ=255)
                                                                                        taCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAAt        <  1:3611868/100‑1 (MQ=255)
                                                                                        naCATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAAt        >  1:2110014/2‑100 (MQ=255)
                                                                                          cATGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATcc      >  2:3177805/1‑100 (MQ=255)
                                                                                           aTGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCa     <  1:902046/100‑1 (MQ=255)
                                                                                            tGCTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCAt    <  2:1491147/100‑1 (MQ=255)
                                                                                              cTTCTGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGt  >  2:2474060/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATATCGCAAGTGAAAATAATCTACGTGGCGTCAAAATCCATGT  >  NC_000913/4061138‑4061331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A