Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,358,695 A→C intergenic (‑78/+2) cadA ← / ← cadB lysine decarboxylase, acid‑inducible/putative lysine/cadaverine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,358,6950AC98.0% 867.7 / 4.1 248intergenic (‑78/+2)cadA/cadBlysine decarboxylase, acid‑inducible/putative lysine/cadaverine transporter
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (123/119);  total (125/123)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 257 total aligned reads displayed.

AATATTGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGACGA  >  NC_000913/4358598‑4358791
                                                                                                 |                                                                                                
 atatTGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTttcatt                                                                                               <  2:4361977/100‑1 (MQ=255)
  tatTGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTttcatta                                                                                              <  2:4824782/100‑1 (MQ=255)
   atTGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTttcattaa                                                                                             >  1:1113435/1‑100 (MQ=255)
     tGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAAtg                                                                                           >  1:4636691/1‑100 (MQ=255)
     tGCAATAACGTTCATAGGCATATCTCCAGGTAAAAAAGGCCCCTCCGAACACATGGGACAAATTGAAAGGAGGAGCCTCTGAAAATACTTTGCATTGAtg                                                                                           >  2:390365/1‑100 (MQ=255)
       cactAACGTTCATAGTCATATCCCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGACAATACTTTTCATTAAtgtg                                                                                         >  2:1969962/4‑100 (MQ=255)
       cAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAAtgtg                                                                                         >  1:2836738/1‑100 (MQ=255)
       cAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAAtgtg                                                                                         >  1:2339950/1‑100 (MQ=255)
       cAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAAtgtg                                                                                         >  1:1922865/1‑100 (MQ=255)
         aTAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCg                                                                                       >  2:1859864/1‑100 (MQ=255)
            aCGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTa                                                                                    <  1:5126269/100‑1 (MQ=255)
             cGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAg                                                                                   <  2:1869047/100‑1 (MQ=255)
               ttCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGAc                                                                                 <  2:5012295/100‑1 (MQ=255)
                   tAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCgg                                                                             <  1:1482677/100‑1 (MQ=255)
                    aGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGgt                                                                            <  2:1567102/100‑1 (MQ=255)
                     gTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGgtg                                                                           >  1:1226535/1‑100 (MQ=255)
                     gTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACAGGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGgtg                                                                           >  2:4163636/1‑100 (MQ=255)
                      tCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGgtgt                                                                          >  1:2413961/1‑100 (MQ=255)
                       cATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGtgtg                                                                         >  2:5116368/1‑100 (MQ=255)
                       cATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGCTAGACGCGGtgtg                                                                         >  2:4305302/1‑100 (MQ=255)
                       cATACCCCCAGGTAAAAAAGGCCCCTCCCCACACATGGGACAAAATGCAAGGCGGACCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGtgtg                                                                         <  1:2228012/100‑1 (MQ=255)
                        atatCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTgg                                                                        <  2:3837426/100‑1 (MQ=255)
                         tatCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGt                                                                       <  2:1695002/100‑1 (MQ=255)
                         tatCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGt                                                                       >  1:2733109/1‑100 (MQ=255)
                         tatCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGt                                                                       >  2:459799/1‑100 (MQ=255)
                         tatCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGt                                                                       <  1:376626/100‑1 (MQ=255)
                           tctcCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTa                                                                     >  2:3011077/1‑100 (MQ=255)
                            ctcCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTAt                                                                    >  1:3788194/1‑100 (MQ=255)
                             tcCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATc                                                                   <  2:1053524/100‑1 (MQ=255)
                               cAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCa                                                                 >  1:4033228/1‑100 (MQ=255)
                               cAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCa                                                                 <  2:2369437/100‑1 (MQ=255)
                               cAGCTAAAAAATTCCCCTCCCAACACATGGGACAAAATGAAAGGATGAGCCTCGGAAAATCCTTTTCATTAATGTGCGTTTGACGCTGTGTGGTTCTcct                                                                 >  2:3098928/1‑99 (MQ=255)
                                aGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCAt                                                                >  1:115612/1‑100 (MQ=255)
                                 ggTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCAtt                                                               >  2:4556203/1‑100 (MQ=255)
                                  gTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTg                                                              <  2:2886992/100‑1 (MQ=255)
                                  gTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTg                                                              <  1:2008408/100‑1 (MQ=255)
                                   tAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGa                                                             <  1:2939391/100‑1 (MQ=255)
                                   tAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGa                                                             >  2:2410371/1‑100 (MQ=255)
                                    aaaaaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAg                                                            >  1:2899611/1‑100 (MQ=255)
                                     aaaaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGt                                                           <  1:4363335/100‑1 (MQ=255)
                                      aaaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTg                                                          >  1:2074702/1‑100 (MQ=255)
                                       aaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTgg                                                         >  2:1426279/1‑100 (MQ=255)
                                       aaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTgg                                                         <  1:4216513/100‑1 (MQ=255)
                                       aaaGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTgg                                                         <  1:1748475/100‑1 (MQ=255)
                                         aGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGct                                                       <  2:1537520/100‑1 (MQ=255)
                                         aGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGct                                                       >  1:847012/1‑100 (MQ=255)
                                             cccTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTgg                                                   >  1:4402039/1‑100 (MQ=255)
                                             cccTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTgg                                                   <  1:4153874/100‑1 (MQ=255)
                                             cccTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTgg                                                   >  1:4025952/1‑100 (MQ=255)
                                              ccTCCCAACACATGGGACAAAATGAAAGGAGGTGCCTCAGAAAATAATTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGgc                                                  >  1:4946942/1‑100 (MQ=255)
                                                tCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGgcgc                                                >  1:4307879/1‑100 (MQ=255)
                                                 cccAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCt                                               <  2:1245638/100‑1 (MQ=255)
                                                  ccAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTc                                              <  2:215217/100‑1 (MQ=255)
                                                   cAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCg                                             <  2:224357/100‑1 (MQ=255)
                                                     acacATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTg                                           <  2:654166/100‑1 (MQ=255)
                                                     acacATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTg                                           <  1:2212502/100‑1 (MQ=255)
                                                     acacATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTg                                           <  2:3780337/100‑1 (MQ=255)
                                                      cacaTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGc                                          <  1:5480720/100‑1 (MQ=255)
                                                       acaTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCa                                         <  1:4475553/100‑1 (MQ=255)
                                                        caTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCAt                                        <  2:4877658/100‑1 (MQ=255)
                                                        caTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCAt                                        >  2:894410/1‑100 (MQ=255)
                                                         aTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCAtt                                       >  1:1593005/1‑100 (MQ=255)
                                                         aTGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCAtt                                       <  2:2563669/100‑1 (MQ=255)
                                                           gggACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCAtttt                                     >  2:801287/1‑100 (MQ=255)
                                                            ggACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTg                                    >  1:95392/1‑100 (MQ=255)
                                                            ggACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTg                                    >  1:420452/1‑100 (MQ=255)
                                                             gACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGc                                   <  1:5388625/100‑1 (MQ=255)
                                                                aaaaTGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAg                                <  2:2805181/100‑1 (MQ=255)
                                                                 aaaTGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGc                               >  1:3948315/1‑100 (MQ=255)
                                                                 aaaTGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGc                               >  2:1667001/1‑100 (MQ=255)
                                                                  aaTGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCg                              <  1:1410059/100‑1 (MQ=255)
                                                                   aTGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGt                             <  1:5371202/100‑1 (MQ=255)
                                                                    tGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTa                            >  1:4595657/1‑100 (MQ=255)
                                                                    tGAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTa                            >  1:1053085/1‑100 (MQ=255)
                                                                     gAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAg                           <  2:3036074/100‑1 (MQ=255)
                                                                     gAAAGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAg                           >  1:2699203/1‑100 (MQ=255)
                                                                      aaaGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGa                          <  1:1319869/100‑1 (MQ=255)
                                                                      aaaGGAGGAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGa                          >  2:2037425/1‑100 (MQ=255)
                                                                        aggaggAGCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAAc                        <  2:2604290/100‑1 (MQ=255)
                                                                          gaggagCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACAt                      <  1:3660291/100‑1 (MQ=255)
                                                                            ggagCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCa                    <  1:3262431/100‑1 (MQ=255)
                                                                            ggagCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCa                    >  2:3988831/1‑100 (MQ=255)
                                                                             gggCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAg                   <  2:5458351/98‑1 (MQ=255)
                                                                             gagCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAg                   <  1:4825167/100‑1 (MQ=255)
                                                                              agCCTCGGAAAATACTTTTCATTAATTTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTATGGCGCTCGTGCATTTTGCGAGCGTAGAACATAAgg                  >  2:1100788/1‑100 (MQ=255)
                                                                               gCCTCGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGa                 >  2:3637812/1‑100 (MQ=255)
                                                                                 cTCGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATa               <  1:5422923/100‑1 (MQ=255)
                                                                                   cGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAAt             <  2:2687448/100‑1 (MQ=255)
                                                                                   cGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAAt             <  2:5280318/100‑1 (MQ=255)
                                                                                   cGGAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAAt             <  1:4778669/100‑1 (MQ=255)
                                                                                   cGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAAt             >  1:2503085/1‑100 (MQ=255)
                                                                                     gAAAATACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCa           >  1:630899/1‑100 (MQ=255)
                                                                                     gAAAATACTTTTCATTAATGGGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCa           <  2:559081/100‑1 (MQ=255)
                                                                                        aaTACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGc        <  1:3551289/100‑1 (MQ=255)
                                                                                          tACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTg      <  2:4067863/100‑1 (MQ=255)
                                                                                          tACTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTg      >  2:340834/1‑100 (MQ=255)
                                                                                            cTTTTCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGAc    <  1:1642735/100‑1 (MQ=255)
                                                                                             ttttCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGACg   <  1:3141515/100‑1 (MQ=255)
                                                                                             ttttCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGACg   <  2:1210250/100‑1 (MQ=255)
                                                                                              tttCATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGACGa  >  1:5193032/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AATATTGCAATAACGTTCATAGTCATATCTCCAGGTAAAAAAGGCCCCTCCCAACACATGGGACAAAATGAAAGGAGGAGCCTCGGAAAATACTTTTAATTAATGTGCGTTAGACGCGGTGTGGTTATCCATTGAGTGGCTCTGGCGCTCGTGCATTTTGCGAGCGTAGAACATCAGGATAATCAGGCTGACGA  >  NC_000913/4358598‑4358791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A