Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 175,540 T→G L145W (TTG→TGG)  clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913175,5400TG99.2% 418.8 / ‑5.4 120L145W (TTG→TGG) clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (57/62);  total (58/62)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 122 total aligned reads displayed.

GAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGC  >  NC_000913/175443‑175635
                                                                                                 |                                                                                               
gAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGGATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGTAGGCATGGTGTggg                                                                                               >  2:328834/1‑100 (MQ=255)
 aaGGGGCGCTGGAAGATCAACGTTCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTgggg                                                                                              <  2:848661/100‑1 (MQ=255)
  aGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGgggg                                                                                             >  2:1001866/1‑100 (MQ=255)
   ggggCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGgc                                                                                            >  2:921897/1‑100 (MQ=255)
        gcTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGaa                                                                                       >  2:140421/1‑100 (MQ=255)
         cTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAg                                                                                      <  1:2958101/100‑1 (MQ=255)
         cTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAg                                                                                      >  1:1441697/1‑100 (MQ=255)
           ggAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAggg                                                                                    >  1:4047320/1‑100 (MQ=255)
           ggAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAggg                                                                                    >  1:3582200/1‑100 (MQ=255)
           ggAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAggg                                                                                    <  2:2465899/100‑1 (MQ=255)
            gAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGc                                                                                   <  1:2726918/100‑1 (MQ=255)
             aaGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGcc                                                                                  <  1:1943824/100‑1 (MQ=255)
              aGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCa                                                                                 >  1:3549744/1‑100 (MQ=255)
               gATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCaa                                                                                >  1:1005297/1‑100 (MQ=255)
               gATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCaa                                                                                >  1:533862/1‑100 (MQ=255)
               gATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCaa                                                                                >  1:1925119/1‑100 (MQ=255)
               gATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCaa                                                                                >  2:1815921/1‑100 (MQ=255)
                 tctaCGTCCCGTTAGCTGGTGGCGTGTATTGGCGGTGTAGTTCTTTGGCGGGTTGGTGTCACCCGGCGGAGGCATGGTGTGGGGGCGCAAAGGGCCAAcc                                                                              <  2:2133303/97‑1 (MQ=255)
                   aaCGTCCCGTTCGCTTGTGGCGTGTATTGCCGGTGCAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAGGGGCCAACCGt                                                                            >  2:2573582/1‑100 (MQ=255)
                   aaCGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGt                                                                            >  2:2438390/1‑100 (MQ=255)
                    aCGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTg                                                                           <  1:3782054/100‑1 (MQ=255)
                    aCGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTg                                                                           >  2:873393/1‑100 (MQ=255)
                    aCGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTg                                                                           >  2:2416037/1‑100 (MQ=255)
                     cGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGc                                                                          <  1:1724238/100‑1 (MQ=255)
                     cGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGc                                                                          <  2:752082/100‑1 (MQ=255)
                      gTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCa                                                                         <  1:673173/100‑1 (MQ=255)
                        cccGTTCGCGGGGGGCCTGTATTGCGTGGGACGTTCTTACGCGGGCCGGGGAGATTCGGGTGAGGAAGGGAGTGGGGGCGAGAAGGGCCAACCGTGCAGa                                                                       >  2:539054/1‑100 (MQ=255)
                          cGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATc                                                                     >  1:1989769/1‑100 (MQ=255)
                          cGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATc                                                                     >  1:1989754/1‑100 (MQ=255)
                           gTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATcg                                                                    <  2:1110944/100‑1 (MQ=255)
                           gTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATcg                                                                    >  2:1253930/1‑100 (MQ=255)
                             tCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATcggc                                                                  <  1:2489549/100‑1 (MQ=255)
                               gCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATcggcgg                                                                <  2:522686/100‑1 (MQ=255)
                               gCTGGTGGCGTGTATTGCCGGTGAAGTTCTTCGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATcggcgg                                                                <  1:1266757/100‑1 (MQ=255)
                                cTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGt                                                               <  2:1416415/100‑1 (MQ=255)
                                cTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGt                                                               <  2:1322192/100‑1 (MQ=255)
                                cTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGt                                                               >  2:2796800/1‑100 (MQ=255)
                                cTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGt                                                               <  2:530988/100‑1 (MQ=255)
                                 tggtggCGTGTACTGCCGGGGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGTGGGCATGGTCTGGGTGCGGGAGGGGCCAACCGTGCTGATCGGCGGTa                                                              <  2:1828379/100‑1 (MQ=255)
                                  ggtggGGGGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTaa                                                             <  2:1081447/100‑1 (MQ=255)
                                  ggtggCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTaa                                                             >  1:498195/1‑100 (MQ=255)
                                  ggtggCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTaa                                                             >  1:72021/1‑100 (MQ=255)
                                   gtggCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAAc                                                            <  1:2692004/100‑1 (MQ=255)
                                    tggCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACa                                                           <  1:2980315/100‑1 (MQ=255)
                                       cGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTg                                                        >  2:2879697/1‑100 (MQ=255)
                                        gtgtATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACACTgg                                                       >  2:942876/1‑100 (MQ=255)
                                         tgtATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACATTCGGCGGAGGCAAGGTGTGGAGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGc                                                      <  1:3877254/100‑1 (MQ=255)
                                         tgtATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGc                                                      <  1:567709/100‑1 (MQ=255)
                                         tgtATTGCCGGTGAAGTTCCTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGc                                                      <  1:3074762/100‑1 (MQ=255)
                                          gtATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGcc                                                     >  2:2519056/1‑100 (MQ=255)
                                           tATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCg                                                    >  2:900667/1‑100 (MQ=255)
                                           tATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCg                                                    <  1:325987/100‑1 (MQ=255)
                                           tATTGCCGGTGAAGTTCTTTGGCGAGCTGGAGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCg                                                    >  2:1779132/1‑100 (MQ=255)
                                             ttGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTa                                                  <  2:713398/100‑1 (MQ=255)
                                              tGCCGGTGAAGTTCTTTGGCGGGCTGGGGACCCTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTAt                                                 >  2:717108/1‑100 (MQ=255)
                                               gCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATg                                                <  2:308361/100‑1 (MQ=255)
                                               gCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATg                                                <  1:3095466/100‑1 (MQ=255)
                                                ccGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATgg                                               <  1:1296997/100‑1 (MQ=255)
                                                 cGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGt                                              >  1:993005/1‑100 (MQ=255)
                                                  ggTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTg                                             <  2:3068919/100‑1 (MQ=255)
                                                    tGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCt                                           <  2:2897720/100‑1 (MQ=255)
                                                     gAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCtt                                          >  1:1295763/1‑100 (MQ=255)
                                                      aaGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTg                                         <  1:1021218/100‑1 (MQ=255)
                                                       aGTTCTTTGGCGGGCTGGGGACACTTGGCGTTGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCAGCGGTAACATTGGCCGTATGGTGCTTGa                                        <  2:3128179/100‑1 (MQ=255)
                                                        gTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGat                                       >  2:2475144/1‑100 (MQ=255)
                                                        gTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGat                                       >  2:513118/1‑100 (MQ=255)
                                                        gTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGat                                       >  1:2497305/1‑100 (MQ=255)
                                                            tttGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATAttt                                   <  1:3962107/100‑1 (MQ=255)
                                                            tttGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATAttt                                   >  2:2294923/1‑100 (MQ=255)
                                                             ttGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATAtttt                                  <  1:673304/100‑1 (MQ=255)
                                                              tGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTc                                 <  1:4648/100‑1 (MQ=255)
                                                               ggCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTGTGGTGCTGGATATTTTcc                                >  2:205833/1‑100 (MQ=255)
                                                                gCGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCg                               <  1:1732463/100‑1 (MQ=255)
                                                                 cGGGCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGc                              >  1:2219840/1‑100 (MQ=255)
                                                                  gggCTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGcc                             <  1:2571387/100‑1 (MQ=255)
                                                                     cTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGa                          <  1:1991589/100‑1 (MQ=255)
                                                                     cTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGa                          >  1:3377152/1‑100 (MQ=255)
                                                                     cTGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGa                          >  2:3581033/1‑100 (MQ=255)
                                                                      tGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGaa                         <  1:3864462/100‑1 (MQ=255)
                                                                      tGGGGACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGaa                         <  1:941639/100‑1 (MQ=255)
                                                                      cccccacacTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGaa                         >  1:1920506/6‑100 (MQ=255)
                                                                       ggggACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGaaa                        >  1:1566122/1‑100 (MQ=255)
                                                                       ggggACACTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGaaa                        >  1:3032221/1‑100 (MQ=255)
                                                                         ggatacTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAgg                      <  2:1573954/96‑1 (MQ=255)
                                                                           acacTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTg                    <  1:311058/100‑1 (MQ=255)
                                                                             acTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGAc                  >  1:1092428/1‑100 (MQ=255)
                                                                              ntCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACg                 >  1:1394193/2‑100 (MQ=255)
                                                                              cTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACg                 >  2:1154888/1‑100 (MQ=255)
                                                                              cTCGGCGGAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACg                 >  1:1582059/1‑100 (MQ=255)
                                                                                cggcggAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGaa               <  2:713808/100‑1 (MQ=255)
                                                                                cggcggAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGaa               <  1:1277543/100‑1 (MQ=255)
                                                                                 ggcggAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAg              >  1:2382127/1‑100 (MQ=255)
                                                                                  gcggAGGCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGc             <  1:1845676/100‑1 (MQ=255)
                                                                                       ggCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGcc        <  1:1592075/100‑1 (MQ=255)
                                                                                       ggCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGcc        >  1:3426330/1‑100 (MQ=255)
                                                                                        gCATGGTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCa       <  1:3733339/100‑1 (MQ=255)
                                                                                            ggTGTGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACg   >  1:1919080/1‑100 (MQ=255)
                                                                                             gtgtGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACgc  >  2:2924385/1‑100 (MQ=255)
                                                                                             gtgtGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACgc  <  2:1762916/100‑1 (MQ=255)
                                                                                             gtgtGGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACgc  <  1:2513808/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGCGGGCTGGGGACACTCGGCGGAGGCATGGTGTTGGGGCGCGAAGGGCCAACCGTGCAGATCGGCGGTAACATTGGCCGTATGGTGCTTGATATTTTCCGCCTGAAAGGTGACGAAGCTCGCCATACGC  >  NC_000913/175443‑175635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A