Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 547,728 T→G L350R (CTT→CGT)  fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913547,7280TG98.8% 563.4 / ‑2.0 160L350R (CTT→CGT) fdrAputative NAD(P)‑binding acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (73/85);  total (73/87)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 160 total aligned reads displayed.

TCACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATT  >  NC_000913/547632‑547823
                                                                                                |                                                                                               
 cACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGcc                                                                                             >  2:508086/1‑100 (MQ=255)
 cACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGcc                                                                                             <  1:3439916/100‑1 (MQ=255)
 cACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGcc                                                                                             >  2:3324419/1‑100 (MQ=255)
 cACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGcc                                                                                             <  1:1633382/100‑1 (MQ=255)
   cGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCgg                                                                                           <  1:1233521/100‑1 (MQ=255)
   cGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCgg                                                                                           <  2:1072437/100‑1 (MQ=255)
   cGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCgg                                                                                           >  1:1412914/1‑100 (MQ=255)
     gcgcGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGac                                                                                         <  1:3425690/100‑1 (MQ=255)
     gcgcGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGac                                                                                         <  1:4055194/100‑1 (MQ=255)
      cgcgACGTAACGCAATAGCGCCTGTCAGCATCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGaca                                                                                        <  1:3886469/100‑1 (MQ=255)
        cgACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTTGCTGCCGAAGCGGCGGGATTACGTGCCGGACAcc                                                                                      >  2:204121/1‑100 (MQ=255)
        cgACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACAcc                                                                                      >  2:947002/1‑100 (MQ=255)
         gACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCt                                                                                     >  1:1617270/1‑100 (MQ=255)
          aCGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCtt                                                                                    <  1:3175308/100‑1 (MQ=255)
          aCGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCtt                                                                                    >  1:38462/1‑100 (MQ=255)
           cGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTg                                                                                   <  1:54188/100‑1 (MQ=255)
            gTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTgg                                                                                  >  1:818703/1‑100 (MQ=255)
            gTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTgg                                                                                  >  1:2077457/1‑100 (MQ=255)
            gTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTgg                                                                                  <  1:1400329/100‑1 (MQ=255)
             tAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGc                                                                                 <  1:1482914/100‑1 (MQ=255)
              aaCGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCg                                                                                >  1:902730/1‑100 (MQ=255)
              aaCGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCg                                                                                <  1:1429153/100‑1 (MQ=255)
               aCGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGt                                                                               >  2:3071262/1‑100 (MQ=255)
                cGCAATAGCGCCTGTCAGTAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTg                                                                              <  1:1543980/100‑1 (MQ=255)
                cGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTg                                                                              >  1:759636/1‑100 (MQ=255)
                 gCAATAGCGCCTGTCAGCAGCGGATTTCTTTGCGGTTTGTATACCGGCGGTACGCTGCCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTgg                                                                             <  2:3952895/100‑1 (MQ=255)
                 gCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTgg                                                                             <  2:2754618/100‑1 (MQ=255)
                 gCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTgg                                                                             >  2:3033200/1‑100 (MQ=255)
                  cAATAGCGCCTGTCAGCAGCGGATTTAGTGGCGGTTTGTATTCCGGCGGTACGGTGGCTGCCGAAGCGGCAGGATTGCGTGCAGGGCACCTTGGCGTGGa                                                                            <  2:3676751/100‑1 (MQ=255)
                    aTAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAg                                                                          <  1:2387691/100‑1 (MQ=255)
                    aTAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAg                                                                          >  1:1396079/1‑100 (MQ=255)
                     tAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGc                                                                         <  2:1031759/100‑1 (MQ=255)
                      gatctcTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGcc                                                                        <  1:1830516/95‑1 (MQ=255)
                      aGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGcc                                                                        >  1:751955/1‑100 (MQ=255)
                      aGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGcc                                                                        <  1:680524/100‑1 (MQ=255)
                       gcgcCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCcg                                                                       <  2:2417536/100‑1 (MQ=255)
                         gcCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCcgac                                                                     <  1:4042086/100‑1 (MQ=255)
                          ccTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCcgacg                                                                    <  2:247197/100‑1 (MQ=255)
                          ccTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCcgacg                                                                    <  1:1368138/100‑1 (MQ=255)
                           cTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGccagatc                                                                   >  2:1800982/1‑95 (MQ=255)
                               cagcagCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATAcc                                                               >  1:939999/1‑100 (MQ=255)
                               cagcagCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATAcc                                                               <  2:131445/100‑1 (MQ=255)
                                 gcagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCa                                                             <  1:2766431/100‑1 (MQ=255)
                                 gcagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCa                                                             <  2:2132429/100‑1 (MQ=255)
                                 gcagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCa                                                             >  1:1015142/1‑100 (MQ=255)
                                  cagcGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCAt                                                            <  1:2453341/100‑1 (MQ=255)
                                     cGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCaa                                                         >  1:3006766/1‑100 (MQ=255)
                                      ggATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAAc                                                        >  1:29276/1‑100 (MQ=255)
                                       gATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGGGCCGGACACCTTTGCGTGGAAGCCGACGATACCCATCAACa                                                       <  2:2381515/100‑1 (MQ=255)
                                        atttatttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACAt                                                      <  1:2443420/100‑1 (MQ=255)
                                        atttatttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACAt                                                      <  1:966318/100‑1 (MQ=255)
                                         tttatttGCGGTTTGTATACTGGCGGTACGCTGGCTGCGGAAGGGGCGGGGTTACGTGCCGGATACCTTGTAGTGGAAGCCGACGATACCCGTCAACATg                                                     >  2:179596/1‑100 (MQ=255)
                                          ttatttGCGTTTTGTATACCGGCGGGACGCTTGGTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATgg                                                    <  2:51507/100‑1 (MQ=255)
                                          ttatttGCGGTTGGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATgg                                                    <  2:2223734/100‑1 (MQ=255)
                                            atttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCa                                                  <  2:1154830/100‑1 (MQ=255)
                                            atttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCa                                                  >  2:3877360/1‑100 (MQ=255)
                                            atttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCa                                                  <  1:2889475/100‑1 (MQ=255)
                                             tttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                                                 <  1:1127683/100‑1 (MQ=255)
                                             tttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                                                 >  1:2319106/1‑100 (MQ=255)
                                             tttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                                                 >  1:1359447/1‑100 (MQ=255)
                                             tttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                                                 <  1:118869/100‑1 (MQ=255)
                                             tttGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCat                                                 >  1:2669720/1‑100 (MQ=255)
                                               tGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCatga                                               >  2:1639633/1‑100 (MQ=255)
                                                 cGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCatgatg                                             >  2:803764/1‑100 (MQ=255)
                                                 cGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCatgatg                                             <  1:2460968/100‑1 (MQ=255)
                                                  ggTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGc                                            <  1:190179/100‑1 (MQ=255)
                                                   gTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCt                                           <  2:1763171/100‑1 (MQ=255)
                                                   gTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCt                                           <  1:3621570/100‑1 (MQ=255)
                                                   gTTGGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAATATGGCATGATGCt                                           >  2:3668861/1‑100 (MQ=255)
                                                     ttGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTgg                                         <  1:3970876/100‑1 (MQ=255)
                                                        tataCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACg                                      >  2:3155198/1‑100 (MQ=255)
                                                        tataCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACg                                      >  1:3580766/1‑100 (MQ=255)
                                                         ataCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGc                                     >  1:1820258/1‑100 (MQ=255)
                                                         ataCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGc                                     >  1:524739/1‑100 (MQ=255)
                                                         ataCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGAGACCCATCAACATGGCATGATGCTGGACGc                                     >  1:709415/1‑100 (MQ=255)
                                                           aCCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCg                                   <  2:3146227/100‑1 (MQ=255)
                                                             cggcggTACGCTGGCTGCCGAAGCGGCGTGATTACGTGCCTGACACCTTTGCGTGGAATCCGTCTATACCCATCAACATGGCATGATGCTGGACGCCGAt                                 <  2:1383741/100‑1 (MQ=255)
                                                                 ggTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGcc                             <  1:3093666/100‑1 (MQ=255)
                                                                 ggTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGcc                             >  2:1331930/1‑100 (MQ=255)
                                                                  gTACGCTGGCTGCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGcca                            >  2:797013/1‑100 (MQ=255)
                                                                  gTACGCTGGCTGCCGAAGCGGCGGGAGTACGTGCCGGACACCTTGGCGTGGGAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGcca                            >  2:2415831/1‑100 (MQ=255)
                                                                       ctggctgCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGa                       >  1:1548574/1‑100 (MQ=255)
                                                                       ctggctgCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGa                       >  2:3623976/1‑100 (MQ=255)
                                                                       ctggctgACGAAGCGGCGGGATTGCGTGCCGGACATCTTGGCTTGGAAGCCGCCGATACCCATCACCATGGCATGTTGCTGGACGCCGATAGCCACCAGa                       <  2:36494/100‑1 (MQ=255)
                                                                        tggctgCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGat                      >  1:105591/1‑100 (MQ=255)
                                                                           ctgCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGattat                   <  2:2727550/100‑1 (MQ=255)
                                                                             gCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTg                 <  2:2160358/100‑1 (MQ=255)
                                                                             gCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTg                 <  2:671165/100‑1 (MQ=255)
                                                                             gCCGAAGCGGCGGGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTg                 >  2:726605/1‑100 (MQ=255)
                                                                                    cggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCgg          <  1:1120947/100‑1 (MQ=255)
                                                                                    cggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCgg          >  1:1675083/1‑100 (MQ=255)
                                                                                     ggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGc         >  2:2278724/1‑100 (MQ=255)
                                                                                     ggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGc         <  2:1217504/100‑1 (MQ=255)
                                                                                     ggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGc         <  1:3082782/100‑1 (MQ=255)
                                                                                     ggcggGATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGACACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGc         >  1:3475497/1‑100 (MQ=255)
                                                                                      gcggGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCg        <  2:3947872/100‑1 (MQ=255)
                                                                                        gggATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCgat      <  1:1245541/100‑1 (MQ=255)
                                                                                        gggATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCgat      <  1:607499/100‑1 (MQ=255)
                                                                                         ggATTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCgatg     >  2:613634/1‑100 (MQ=255)
                                                                                           aTTACGTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCgatgat   >  1:1459639/1‑100 (MQ=255)
                                                                                                |                                                                                               
TCACGGCGCGACGTAACGCAATAGCGCCTGTCAGCAGCGGATTTATTTGCGGTTTGTATACCGGCGGTACGCTGGCTGCCGAAGCGGCGGGATTACTTGCCGGACACCTTGGCGTGGAAGCCGACGATACCCATCAACATGGCATGATGCTGGACGCCGATAGCCACCAGATTATTGACCTCGGCGATGATT  >  NC_000913/547632‑547823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A