Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 619,006 A→C G251G (GGA→GGC)  fepE → regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,0060AC98.5% 451.1 / ‑1.7 132G251G (GGA→GGC) fepEregulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (53/77);  total (53/79)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 133 total aligned reads displayed.

AAAGAAAAACTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGA  >  NC_000913/618908‑619101
                                                                                                  |                                                                                               
aaagaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGaa                                                                                                >  2:3607949/1‑100 (MQ=255)
 aagaaaaaCTGGCTCAGCAGCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACGTTGCCAACGCGGCAGGCAt                                                                                               <  2:646854/100‑1 (MQ=255)
  agaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAAtt                                                                                              <  1:566420/100‑1 (MQ=255)
   gaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAACCATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTa                                                                                             <  2:3944197/100‑1 (MQ=255)
   gaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTa                                                                                             <  1:3727492/100‑1 (MQ=255)
   gaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTa                                                                                             >  1:2539350/1‑100 (MQ=255)
   gaaaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTa                                                                                             <  1:223162/100‑1 (MQ=255)
     aaaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCATTCGACATTGCCAACGCGGCAGGCATTaaa                                                                                           >  2:3056234/1‑100 (MQ=255)
      aaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTaaaa                                                                                          >  2:83850/1‑100 (MQ=255)
      aaaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTaaaa                                                                                          <  1:743964/100‑1 (MQ=255)
       aaCTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTaaaaa                                                                                         <  1:3420222/100‑1 (MQ=255)
         cTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGc                                                                                       <  1:3892671/100‑1 (MQ=255)
           ggCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGccc                                                                                     >  2:3058496/1‑100 (MQ=255)
            gCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCg                                                                                    <  1:60844/100‑1 (MQ=255)
               cAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGttt                                                                                 <  1:1167554/100‑1 (MQ=255)
               cAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGttt                                                                                 >  1:913250/1‑100 (MQ=255)
                 ggATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTAc                                                                               <  1:1640156/100‑1 (MQ=255)
                 ggATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTAc                                                                               <  1:1729203/100‑1 (MQ=255)
                   aTCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAg                                                                             <  2:229618/100‑1 (MQ=255)
                   aTCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAg                                                                             <  2:1406790/100‑1 (MQ=255)
                   aTCGAATTAAAACAAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAg                                                                             <  2:3275268/100‑1 (MQ=255)
                    tCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGt                                                                            >  2:2146388/1‑100 (MQ=255)
                      gCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGCCATTGCCAACGCGGCAGGCAGTAAAAAGCCCGTTTACAGTaa                                                                          <  1:3857830/100‑1 (MQ=255)
                      gCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCCTTAAAAAGCCCGTTTACAGTaa                                                                          <  1:3410899/100‑1 (MQ=255)
                      gCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTaa                                                                          >  1:2264156/1‑100 (MQ=255)
                      gCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTaa                                                                          >  2:1598899/1‑100 (MQ=255)
                       cATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAAt                                                                         >  1:2298053/1‑100 (MQ=255)
                       cATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAAt                                                                         <  1:2707550/100‑1 (MQ=255)
                        aTTAAAACGAAAAATCAACTTGATGCAAACATTCATCGCCTCAATTATTCACTCGACATTGCCAACGCTGCAGGCATTAAAAAGCCCGTTTACAGTAATg                                                                        <  2:2797225/100‑1 (MQ=255)
                         ttAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATgg                                                                       >  2:3325673/1‑100 (MQ=255)
                         ttAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATgg                                                                       >  1:1731203/1‑100 (MQ=255)
                         ttAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATgg                                                                       <  1:2100653/100‑1 (MQ=255)
                            aaaCGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCa                                                                    <  1:196532/100‑1 (MQ=255)
                            aaaCGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCa                                                                    <  2:1516593/100‑1 (MQ=255)
                             aaCGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAg                                                                   <  1:2213787/100‑1 (MQ=255)
                             aaCGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAg                                                                   <  1:3686488/100‑1 (MQ=255)
                              aacaaaaaTCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAgg                                                                  <  2:852094/97‑1 (MQ=255)
                                  aaaaTCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGt                                                              <  2:2262435/100‑1 (MQ=255)
                                    aaTCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTa                                                            <  1:1125150/100‑1 (MQ=255)
                                     aTCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaa                                                           >  1:3578130/1‑100 (MQ=255)
                                      tCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa                                                          <  1:3330041/100‑1 (MQ=255)
                                      tCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa                                                          <  2:2717079/100‑1 (MQ=255)
                                      tCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTaaa                                                          <  2:2727971/100‑1 (MQ=255)
                                       cAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAg                                                         <  1:3149940/100‑1 (MQ=255)
                                       cAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAg                                                         <  1:3149968/100‑1 (MQ=255)
                                       cAACTTGATGCAAACAGTCAGCGACTAAATTATTCTCGCGATATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAg                                                         <  2:280934/100‑1 (MQ=255)
                                        aaCTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAACGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGa                                                        <  2:1622695/100‑1 (MQ=255)
                                           ttGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGa                                                     >  2:2630691/1‑100 (MQ=255)
                                             gATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAcc                                                   >  1:1478546/1‑100 (MQ=255)
                                              aTGCACACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAACAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAccc                                                  <  1:3869568/100‑1 (MQ=255)
                                              aTGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAccc                                                  <  1:3310112/100‑1 (MQ=255)
                                              aTGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTCTACAGTAATGGTCAGGCCGTTAAAGATGAccc                                                  <  1:3310983/100‑1 (MQ=255)
                                               tGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAcccc                                                 <  2:3929159/100‑1 (MQ=255)
                                               tGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAcccc                                                 >  1:3645678/1‑100 (MQ=255)
                                                 cAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGa                                               >  2:3309002/1‑100 (MQ=255)
                                                 cAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGa                                               >  2:2089884/1‑100 (MQ=255)
                                                 cAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTATACAGTAATGGTCAGGCCGTTAAAGATGACCCCGa                                               >  2:3950987/1‑100 (MQ=255)
                                                   aaCATTCAGCGCCTCAATTATTCACGCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAtt                                             <  1:2218591/100‑1 (MQ=255)
                                                    aCATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAttt                                            <  1:493096/100‑1 (MQ=255)
                                                    aCATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGAttt                                            <  1:513279/100‑1 (MQ=255)
                                                       ttCGGCGCTTCAATTATTCACTCGCCATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTc                                         <  2:1894801/100‑1 (MQ=255)
                                                       ttCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTc                                         <  2:2047876/100‑1 (MQ=255)
                                                       ttCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTc                                         <  2:2249322/100‑1 (MQ=255)
                                                        tCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCt                                        <  1:1934571/100‑1 (MQ=255)
                                                         caacgcCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTAACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTa                                       <  1:3466704/97‑1 (MQ=255)
                                                         cAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTa                                       >  1:1287295/1‑100 (MQ=255)
                                                         cAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTa                                       <  2:28843/100‑1 (MQ=255)
                                                          aGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTAt                                      <  1:1179552/100‑1 (MQ=255)
                                                           gcgcCTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTAtt                                     >  1:2558220/1‑100 (MQ=255)
                                                             gcCTCAAGTATTCACTCCACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTtc                                   <  1:259148/100‑1 (MQ=255)
                                                              ccTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTtct                                  >  1:3997457/1‑100 (MQ=255)
                                                               cTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTtctc                                 >  1:2637527/1‑100 (MQ=255)
                                                               cTCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAACCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTtctc                                 >  2:3126327/1‑100 (MQ=255)
                                                                tCAATTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTtctct                                >  1:2695313/1‑100 (MQ=255)
                                                                  aaTTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTTTTTTTTTCTCg                              >  2:2652375/1‑100 (MQ=255)
                                                                  aaTTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCg                              >  1:3023854/1‑100 (MQ=255)
                                                                  aaTTATTCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCg                              >  1:2880323/1‑100 (MQ=255)
                                                                   attattCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCgg                             <  2:3609467/100‑1 (MQ=255)
                                                                   attattCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCgg                             <  1:4003956/100‑1 (MQ=255)
                                                                    ttattCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCTTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCggn                            <  1:829589/100‑2 (MQ=255)
                                                                     tattCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTg                           <  1:2821150/100‑1 (MQ=255)
                                                                      attCACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGc                          <  1:548086/100‑1 (MQ=255)
                                                                       ttCACTCGACATTGCCAACGCGGCAGGCATTAACAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCa                         <  1:4034272/100‑1 (MQ=255)
                                                                         cACTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGa                       >  2:652388/1‑100 (MQ=255)
                                                                          aCTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGAc                      >  1:2867282/1‑100 (MQ=255)
                                                                           cTCGACATTGCCAACGCGGCAGGCATTAAAAATCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACg                     <  1:3740033/100‑1 (MQ=255)
                                                                           cTCGACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACg                     >  2:3441758/1‑100 (MQ=255)
                                                                            tctaCATTGCCAACGCTATCGGCAATAAAAAGCCCACTTACAAGAATTATCAGGCCGTTAATGTTGACCCCGATTTTCCTATTTCTCTCGGTGCAGACgg                    <  2:1109960/97‑1 (MQ=255)
                                                                              gACATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTa                  >  2:2615290/1‑100 (MQ=255)
                                                                               aCATTGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTAt                 >  2:76593/1‑100 (MQ=255)
                                                                                  ttGCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGa              <  1:408848/100‑1 (MQ=255)
                                                                                    gCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGAGCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAAc            <  1:3538111/100‑1 (MQ=255)
                                                                                    gCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAAc            <  1:2307222/100‑1 (MQ=255)
                                                                                    gCCAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAAc            <  2:1963121/100‑1 (MQ=255)
                                                                                     ccAACGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACg           <  1:1461831/100‑1 (MQ=255)
                                                                                       aaCGCGGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCa         <  1:1848228/100‑1 (MQ=255)
                                                                                         cgcgGCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCaaa       >  2:2764326/1‑100 (MQ=255)
                                                                                             gCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTTTTTTTTTTTCGGTGCAGACGGTATTGAACGCAAACTgg   >  2:512482/1‑100 (MQ=255)
                                                                                             gCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTgg   <  2:1232734/100‑1 (MQ=255)
                                                                                             gCAGGCATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTgg   >  2:1830962/1‑100 (MQ=255)
                                                                                                  |                                                                                               
AAAGAAAAACTGGCTCAGGATCGCATTAAAACGAAAAATCAACTTGATGCAAACATTCAGCGCCTCAATTATTCACTCGACATTGCCAACGCGGCAGGAATTAAAAAGCCCGTTTACAGTAATGGTCAGGCCGTTAAAGATGACCCCGATTTTTCTATTTCTCTCGGTGCAGACGGTATTGAACGCAAACTGGA  >  NC_000913/618908‑619101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A