Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 960,657 C→T L132L (CTG→TTG)  ycaL → putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913960,6570CT99.2% 445.9 / ‑4.6 130L132L (CTG→TTG) ycaLputative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (58/70);  total (58/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 130 total aligned reads displayed.

ACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGC  >  NC_000913/960561‑960751
                                                                                                |                                                                                              
aCCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTgg                                                                                             >  1:3200218/1‑100 (MQ=255)
 ccAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTggg                                                                                            >  1:3447909/1‑100 (MQ=255)
  cAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAGTGACAACGAAATTGAAGGCGTTTTGGGc                                                                                           <  2:2130219/100‑1 (MQ=255)
    gcggtgTCAAGGCATGGGCGAGGGGGGAGGGCTGTGTGCGTGGCGACAGTGGCCTGATGGGCAGGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCa                                                                                         <  2:3336360/95‑1 (MQ=255)
     cGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCAt                                                                                        <  2:2346403/100‑1 (MQ=255)
     cGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCCAt                                                                                        <  2:3177187/100‑1 (MQ=255)
      gACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATg                                                                                       >  2:2723752/1‑100 (MQ=255)
      gACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATg                                                                                       <  2:1330133/100‑1 (MQ=255)
         gTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAAc                                                                                    >  1:1335400/1‑100 (MQ=255)
         gTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAAc                                                                                    <  2:669560/100‑1 (MQ=255)
            aaCGCATGGGCGATGGCGAACGGCTGTGTACGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTgg                                                                                 <  1:3732783/100‑1 (MQ=255)
             aCGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTggg                                                                                >  2:60073/1‑100 (MQ=255)
               gCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGcc                                                                              >  2:779017/1‑100 (MQ=255)
               gCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGcc                                                                              >  1:2414295/1‑100 (MQ=255)
                 aTGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCAc                                                                            >  1:762709/1‑100 (MQ=255)
                  tGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACg                                                                           <  2:2874079/100‑1 (MQ=255)
                   gggCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGt                                                                          >  2:1392503/1‑100 (MQ=255)
                    ggCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTc                                                                         <  2:724232/100‑1 (MQ=255)
                     gCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATTGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTcg                                                                        <  2:471496/100‑1 (MQ=255)
                     gCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTcg                                                                        >  1:1792609/1‑100 (MQ=255)
                       gATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTcgcg                                                                      <  2:2411538/100‑1 (MQ=255)
                         tGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGtt                                                                    <  1:3764328/100‑1 (MQ=255)
                         tGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGtt                                                                    <  2:241522/100‑1 (MQ=255)
                         tGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGtt                                                                    <  2:89373/100‑1 (MQ=255)
                          ggCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTg                                                                   >  1:2304730/1‑100 (MQ=255)
                           gCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTgg                                                                  <  1:1452676/100‑1 (MQ=255)
                           gCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTgg                                                                  <  1:3616206/100‑1 (MQ=255)
                            cgtccGGCTGTGTTCGTGTCTACAGTGGCCTGATGGAAATGCTGGCTGACAAAGACATTGACGGCGTTTTGGGCCATGCGCTGGGCCCCGTGGCGTTggg                                                                 <  2:588917/96‑1 (MQ=255)
                            cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                 >  2:363282/1‑100 (MQ=255)
                            cGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTggg                                                                 <  2:1847996/100‑1 (MQ=255)
                               aCGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCa                                                              <  1:3464880/100‑1 (MQ=255)
                                cGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTGTTGGGCCATGCACTCGGCCACGTCGCGTTGGGTcgc                                                             >  2:3781342/1‑98 (MQ=255)
                                 ggCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACt                                                            >  1:1090915/1‑100 (MQ=255)
                                 ggCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACt                                                            <  1:3222625/100‑1 (MQ=255)
                                  gCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTc                                                           >  2:3088541/1‑100 (MQ=255)
                                  gCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTc                                                           >  2:3969918/1‑100 (MQ=255)
                                  gCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTc                                                           <  1:3029598/100‑1 (MQ=255)
                                   cTGTGTGCGTGTCAACAGTTGCCTGATTGACATGATGAATGACAACGAAATTGAAGGCGGTTTTGTCCATGAACGGGGCCACGTCTCGTTGGGTCAcgcg                                                          >  2:589125/1‑100 (MQ=255)
                                    tgtgTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgc                                                         <  2:2678233/100‑1 (MQ=255)
                                      tgtTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctg                                                       <  2:509105/100‑1 (MQ=255)
                                        ttCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggc                                                     <  1:868192/100‑1 (MQ=255)
                                        ttCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggc                                                     <  1:1835661/100‑1 (MQ=255)
                                          cGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggctg                                                   <  1:3693567/100‑1 (MQ=255)
                                          cGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCgctggctg                                                   <  1:3638939/100‑1 (MQ=255)
                                           gtgtCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtga                                                  <  1:1712162/100‑1 (MQ=255)
                                              tCTACAGTGGCCTGATGGAGATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCAGTCGCTGGCtgaaat                                               >  2:3658839/1‑100 (MQ=255)
                                                tACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaatga                                             >  1:3748455/1‑100 (MQ=255)
                                                tACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaatga                                             >  1:766952/1‑100 (MQ=255)
                                                 aCAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaatgaa                                            >  1:2029256/1‑100 (MQ=255)
                                                  cAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCtgaaatgaaa                                           <  1:4034764/100‑1 (MQ=255)
                                                   aGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAg                                          <  2:3283577/100‑1 (MQ=255)
                                                   aGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAg                                          <  1:1377289/100‑1 (MQ=255)
                                                   aGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAg                                          <  1:3053098/100‑1 (MQ=255)
                                                     tGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGct                                        <  1:313787/100‑1 (MQ=255)
                                                      ggCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGctt                                       >  1:3000544/1‑100 (MQ=255)
                                                        ncTGATGGACATGATGAATGACAACGAAATTGAAGGCGATTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGcttct                                     >  1:3246812/2‑100 (MQ=255)
                                                          tGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTa                                   >  1:2402148/1‑100 (MQ=255)
                                                          tGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTa                                   <  1:2430737/100‑1 (MQ=255)
                                                          tGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTa                                   <  1:2038336/100‑1 (MQ=255)
                                                          tGATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTa                                   <  1:3171168/100‑1 (MQ=255)
                                                           gATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTAt                                  <  2:1645130/100‑1 (MQ=255)
                                                           gATGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTAt                                  <  2:2668320/100‑1 (MQ=255)
                                                             tGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGc                                >  1:3004098/1‑100 (MQ=255)
                                                             tGGACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGc                                <  1:458648/100‑1 (MQ=255)
                                                              ggACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCg                               >  1:2823996/1‑100 (MQ=255)
                                                               gACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGa                              >  2:3647509/1‑100 (MQ=255)
                                                               gACATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGa                              >  2:8605/1‑100 (MQ=255)
                                                                 cATGATGAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATc                            >  2:2245494/1‑100 (MQ=255)
                                                                  atgatgAATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCg                           <  1:1005928/100‑1 (MQ=255)
                                                                   tgatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGt                          >  1:1733135/1‑100 (MQ=255)
                                                                    gatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGtt                         >  1:1967630/1‑100 (MQ=255)
                                                                    gatgaATGACAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGtt                         >  1:1930747/1‑100 (MQ=255)
                                                                     atgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTg                        >  2:2332545/1‑100 (MQ=255)
                                                                      tgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGc                       <  2:1714539/100‑1 (MQ=255)
                                                                      tgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGc                       <  1:3334496/100‑1 (MQ=255)
                                                                      tgaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGc                       >  2:3112269/1‑100 (MQ=255)
                                                                       gaatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGcc                      <  1:2364953/100‑1 (MQ=255)
                                                                        aatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCg                     <  1:8710/100‑1 (MQ=255)
                                                                        aatgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCg                     <  1:2280386/100‑1 (MQ=255)
                                                                         atgaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGc                    >  2:1031535/1‑100 (MQ=255)
                                                                         atgaCAACGAAATTGAAGGCGTGTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGATGCCGc                    >  2:2544243/1‑100 (MQ=255)
                                                                           gaCAACGAAATTGAAGGCGTTTTGGGCCATGACCTGGGCCACGTCGCGTTGGGTCACGCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAc                  >  2:706159/1‑100 (MQ=255)
                                                                           gaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAc                  <  1:3746525/100‑1 (MQ=255)
                                                                           gaCAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAc                  <  1:1053287/100‑1 (MQ=255)
                                                                             cAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcgc                >  1:4082982/1‑100 (MQ=255)
                                                                             cAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcgc                >  1:1281869/1‑100 (MQ=255)
                                                                             cAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcgc                >  2:1139701/1‑100 (MQ=255)
                                                                             cAACGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCAcgc                >  2:1040850/1‑100 (MQ=255)
                                                                               aCGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGa              >  2:2381281/1‑100 (MQ=255)
                                                                                cGAAATTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGAt             >  1:2044648/1‑100 (MQ=255)
                                                                                    aTTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCa         <  2:2292626/100‑1 (MQ=255)
                                                                                    aTTGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCa         >  2:222215/1‑100 (MQ=255)
                                                                                     ttGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAt        <  2:3874920/100‑1 (MQ=255)
                                                                                     ttGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAt        >  2:2317086/1‑100 (MQ=255)
                                                                                     ttGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAt        >  1:58773/1‑100 (MQ=255)
                                                                                      tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt       >  2:3524906/1‑100 (MQ=255)
                                                                                      tGAAGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCAtt       <  1:1717766/100‑1 (MQ=255)
                                                                                         aGGCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCa    >  2:585776/1‑100 (MQ=255)
                                                                                          ggCGTTTTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGTCGCACGCGATGCCATTTCAg   <  1:3190030/100‑1 (MQ=255)
                                                                                           gCGTTTTGGGCCATGAACTGGGTCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGc  <  2:3865911/100‑1 (MQ=255)
                                                                                                |                                                                                              
ACCAGCGACGTCAACGCATGGGCGATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAATGACAACGAAATTGAAGGCGTTCTGGGCCATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGCGATGCCATTTCAGC  >  NC_000913/960561‑960751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A