Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,036,544 A→C E64A (GAG→GCG)  hyaE → putative HyaA chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,036,5440AC95.3% 403.1 / 5.1 128E64A (GAG→GCG) hyaEputative HyaA chaperone
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (61/60);  total (65/63)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 130 total aligned reads displayed.

ACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCACTT  >  NC_000913/1036447‑1036639
                                                                                                 |                                                                                               
 cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGtt                                                                                              >  1:2226845/1‑100 (MQ=255)
 cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGtt                                                                                              >  2:205941/1‑100 (MQ=255)
 cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGCCGAATTACTGCGCGCGtt                                                                                              >  2:1406683/1‑100 (MQ=255)
  gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGACCCGAAACGCACGCCAGAGATCAACGATAGTCCGGTCATGATTGGCGAATTACTGCGCGCGttt                                                                                             >  2:3817067/1‑100 (MQ=255)
   cAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTc                                                                                            >  1:1038227/1‑100 (MQ=255)
   cAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTc                                                                                            <  2:1052928/100‑1 (MQ=255)
   cAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTc                                                                                            >  2:1504499/1‑100 (MQ=255)
    aaGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTcc                                                                                           <  1:646286/100‑1 (MQ=255)
    aaGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTcc                                                                                           >  2:513169/1‑100 (MQ=255)
    aaGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTcc                                                                                           <  2:3128715/100‑1 (MQ=255)
     aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTccc                                                                                          >  2:2008040/1‑100 (MQ=255)
      gcgcCAGACGTCTTGATGTTATTACGCAGTGACCCGAACCGCACGCCAGAGGTCAGCTATAATCCGGTAATGATTGGCGCAATACTGCGCGCGTTTccag                                                                                         <  2:101904/100‑3 (MQ=255)
      gcgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCg                                                                                         <  1:778042/100‑1 (MQ=255)
       cgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGa                                                                                        >  2:2823858/1‑100 (MQ=255)
       cgcCAGACGGCGTGGTGTTATTAAGCAGTGACCCAAAACGCACGCCAGAGGTCAGAGATAATCCGGTAATGCTTGGCGAATTACTGCGCGCGTTTCCCGa                                                                                        >  2:757242/1‑100 (MQ=255)
        gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGAc                                                                                       <  2:3126414/100‑1 (MQ=255)
        gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGAc                                                                                       <  1:563497/100‑1 (MQ=255)
         ccAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACt                                                                                      >  1:1517423/1‑100 (MQ=255)
          cAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACta                                                                                     <  2:3893582/100‑1 (MQ=255)
          cAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACta                                                                                     >  1:727912/1‑100 (MQ=255)
          cAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACta                                                                                     <  1:52748/100‑1 (MQ=255)
                gCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATg                                                                               >  1:407316/1‑100 (MQ=255)
                  ggggtgTTATTAAACGGTGAGCGGAAACGCACGCTAGCGGTCAGCGATAATCCGTTAATGATTTTCGAATTACTGCGCGTTTGGTCCGACTATACATGGc                                                                             <  2:1154753/98‑1 (MQ=255)
                   tggtgTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCa                                                                            >  2:2998770/1‑100 (MQ=255)
                     gtgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAgg                                                                          >  2:1244061/1‑100 (MQ=255)
                     gtgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAgg                                                                          >  2:3051201/1‑100 (MQ=255)
                      tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGt                                                                         >  1:2280671/1‑100 (MQ=255)
                       gtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTg                                                                        >  1:1955665/1‑100 (MQ=255)
                       gtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTg                                                                        >  1:3092043/1‑100 (MQ=255)
                       gtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTg                                                                        >  2:3791084/1‑100 (MQ=255)
                         tattaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGc                                                                      >  2:2185874/1‑100 (MQ=255)
                         tattaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGc                                                                      <  2:2153560/100‑1 (MQ=255)
                         tattaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGc                                                                      <  2:1958870/100‑1 (MQ=255)
                         tattaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGc                                                                      >  2:691258/1‑100 (MQ=255)
                             aaGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGCATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGAtt                                                                  >  2:1415598/1‑100 (MQ=255)
                             aaGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGAtt                                                                  >  1:2981611/1‑100 (MQ=255)
                              agcagcGCCCCGAAACGCACGCGAGAGGTCAGCGATAGTCCGGTAATGATTGGCGAATTACTGCGAGCGCTTCCCGACTATACATGGCCGGTGGCGATTg                                                                 >  2:1524712/1‑100 (MQ=255)
                              aGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTg                                                                 >  2:1434152/1‑100 (MQ=255)
                               gCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGc                                                                >  1:868506/1‑100 (MQ=255)
                               gCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGATGGCGATTGc                                                                <  2:3682656/100‑1 (MQ=255)
                                 aGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGCCTATACATGGCAGGTGGCGATTGCTg                                                              >  2:593418/1‑100 (MQ=255)
                                  gTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa                                                             >  1:874348/1‑100 (MQ=255)
                                   tGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc                                                            <  1:1528543/100‑1 (MQ=255)
                                   tGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc                                                            <  2:2245774/100‑1 (MQ=255)
                                    gACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc                                                           <  1:1795121/100‑1 (MQ=255)
                                    gACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc                                                           >  1:332385/1‑100 (MQ=255)
                                    gACCCGAAACGCACGCCAGAGGTCACCGATACTCTGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGGGGCGATTGCTGAcc                                                           <  2:270577/100‑1 (MQ=255)
                                      cccGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCtt                                                         >  1:1965523/1‑100 (MQ=255)
                                       ccGAAACGCACGCCAGGGGTCAGGGATAGTCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACGATACATGGCAGGTGGCGATGGCTGACCTTg                                                        <  2:29313/100‑1 (MQ=255)
                                       ccGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg                                                        <  2:2416857/100‑1 (MQ=255)
                                       ccGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg                                                        >  1:2016654/1‑100 (MQ=255)
                                         gAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAg                                                      <  2:3564986/100‑1 (MQ=255)
                                         gAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAg                                                      <  2:2536175/100‑1 (MQ=255)
                                          aaaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGc                                                     <  1:3131292/100‑1 (MQ=255)
                                          aaaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTACCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGc                                                     <  1:1785665/100‑1 (MQ=255)
                                           aaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCa                                                    >  1:602845/1‑100 (MQ=255)
                                              gtacgcCAGAGGTCAGCGATAATCCGGTAATGATTGGTGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCagag                                                 <  2:3639580/98‑1 (MQ=255)
                                                acgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCg                                               <  1:793995/100‑1 (MQ=255)
                                                 cgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGa                                              >  1:3943023/1‑100 (MQ=255)
                                                 cgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGa                                              >  1:3943357/1‑100 (MQ=255)
                                                 cgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGa                                              >  1:294582/1‑100 (MQ=255)
                                                  gcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGaa                                             >  2:121325/1‑100 (MQ=255)
                                                   ccAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAg                                            >  1:1916378/1‑100 (MQ=255)
                                                        ggTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATc                                       <  1:3946243/100‑1 (MQ=255)
                                                         gTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCg                                      >  1:1498297/1‑100 (MQ=255)
                                                           cAGCGATAATCCGGTAATGATTGGCGGATTACTGCGCGCGTTTCCCGACGATACATGGCAGGTGGCTATTGCTGACCTTGAGCAGAGCGAAGCCATCGGc                                    >  2:2828247/1‑100 (MQ=255)
                                                           cAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGc                                    <  1:2997898/100‑1 (MQ=255)
                                                           cAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGc                                    >  2:2569319/1‑100 (MQ=255)
                                                           cAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGc                                    <  1:1039662/100‑1 (MQ=255)
                                                           cAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGc                                    <  1:2784771/100‑1 (MQ=255)
                                                            aGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCg                                   <  2:2947619/100‑1 (MQ=255)
                                                             gCGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGGCATCGGCGa                                  >  2:779588/1‑100 (MQ=255)
                                                              cGATAATCCTGTAATGATTGGCGAATGACTGCTCGCGTTTCCCGACTATACATGGCAGGTTGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGAt                                 <  2:3018497/100‑1 (MQ=255)
                                                              cGATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGAt                                 >  1:928322/1‑100 (MQ=255)
                                                               gATAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATc                                <  1:523403/100‑1 (MQ=255)
                                                                 tAATCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGc                              >  2:2342064/1‑100 (MQ=255)
                                                                   aTCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGCTCGCtt                            >  2:2099385/1‑100 (MQ=255)
                                                                   aTCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCtt                            >  2:3919350/1‑100 (MQ=255)
                                                                   aTCCGGTAATGATTGGCGAATTACGGCGCGAGATTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGGAAAGCGAAGCCATCGGCGATCGCtt                            >  2:971363/1‑100 (MQ=255)
                                                                    tCCGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCttt                           <  1:2831883/100‑1 (MQ=255)
                                                                     ccGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTACACATTGCAGCAGACGATTGCTGACCTTGAGCAGCGCGACCCCATCGGCGATCGCTTTg                          >  2:3737867/1‑100 (MQ=255)
                                                                      cGGTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTgg                         <  2:1095305/100‑1 (MQ=255)
                                                                        gTAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCg                       <  1:1692027/100‑1 (MQ=255)
                                                                         tAATGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGt                      <  1:2100086/100‑1 (MQ=255)
                                                                            tGATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCtt                   <  2:2622004/100‑1 (MQ=255)
                                                                             gATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCttt                  <  2:1909380/100‑1 (MQ=255)
                                                                             gATTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCttt                  >  2:516248/1‑100 (MQ=255)
                                                                              aTTGGCGAATTACTGCGCGCGTTTCTCGACTATACATGGCAGGTGGCGATTGCTGACCTTGGGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTc                 <  1:1325059/100‑1 (MQ=255)
                                                                              aTTGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTc                 <  2:464502/100‑1 (MQ=255)
                                                                                tGGCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGAGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGc               <  1:1018184/100‑1 (MQ=255)
                                                                                 ggCGAATTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGGAGCCATCGGCGATCGCTTTGGCGTCTTTCGCt              <  2:3602064/100‑1 (MQ=255)
                                                                                     aaTTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTcc          <  1:3354379/100‑1 (MQ=255)
                                                                                     aaTTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTcc          <  2:451478/100‑1 (MQ=255)
                                                                                      aTTACTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCt         <  2:72908/100‑1 (MQ=255)
                                                                                         aCTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGcc      <  1:3144781/100‑1 (MQ=255)
                                                                                          cTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCa     >  1:3375199/1‑100 (MQ=255)
                                                                                          cTGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCa     >  2:3116678/1‑100 (MQ=255)
                                                                                           tGCGCGCGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCAc    <  2:3888743/100‑1 (MQ=255)
                                                                                            gcgcgcGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCACt   <  1:2962014/100‑1 (MQ=255)
                                                                                             cgcgcgTTTCCCGACTATACATGGCAGGTGGCGGTTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCACtt  >  1:3387586/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTGAGCAGAGCGAAGCCATCGGCGATCGCTTTGGCGTCTTTCGCTTTCCTGCCACTT  >  NC_000913/1036447‑1036639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A