Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,452	T→G	S231A (TCG→GCG)	holB →	DNA polymerase III, delta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,452	0	T	G	95.3%	341.0 / 5.4	107	S231A (TCG→GCG)	holB	DNA polymerase III, delta prime subunit
Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (48/54); total (50/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 108 total aligned reads displayed.

CCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGTGCTGC  >  NC_000913/1156353‑1156549

ccTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTAtt                                                                                                   >  1:2782317/1‑100 (MQ=255)

    gcgcGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgct                                                                                               >  1:470355/1‑100 (MQ=255)

     cgcgGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctg                                                                                              <  2:2124954/100‑1 (MQ=255)

      gcgGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctgc                                                                                             <  1:1478795/100‑1 (MQ=255)

       cgGCACTTTCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCgctgct                                                                                            <  1:1631984/100‑1 (MQ=255)

         gCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAg                                                                                          <  2:3575653/100‑1 (MQ=255)

          cACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGc                                                                                         >  1:46003/1‑100 (MQ=255)

             tGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGc                                                                                      >  2:2429659/1‑100 (MQ=255)

             tGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGc                                                                                      <  1:413146/100‑1 (MQ=255)

              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGcc                                                                                     <  1:2555171/100‑1 (MQ=255)

              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGcc                                                                                     <  1:1270622/100‑1 (MQ=255)

              ggCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGCTATGCGCTGCTCGCGGcc                                                                                     <  1:3403498/100‑1 (MQ=255)

               gCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGccc                                                                                    <  1:3219471/100‑1 (MQ=255)

               gCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGccc                                                                                    <  2:359599/100‑1 (MQ=255)

                cGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCt                                                                                   <  1:817105/100‑1 (MQ=255)

                cGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCt                                                                                   <  1:817118/100‑1 (MQ=255)

                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCtt                                                                                  >  1:2681655/1‑100 (MQ=255)

                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCtt                                                                                  <  1:262060/100‑1 (MQ=255)

                 gttgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGACCtt                                                                                  <  2:3384488/100‑1 (MQ=255)

                   tgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTaa                                                                                <  1:992709/100‑1 (MQ=255)

                   tgttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTaa                                                                                <  2:2756393/100‑1 (MQ=255)

                    gttTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAAt                                                                               <  2:3166267/100‑1 (MQ=255)

                     tttCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATc                                                                              >  1:1595484/1‑100 (MQ=255)

                     tttCAGGGAGATAACGGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATc                                                                              <  1:2085940/100‑1 (MQ=255)

                      ttaaGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGTCGTTGGCATATAGCATGCCATCGGGCGACTGGTATGCGCTGCTAGCAGCCCTTAATCa                                                                             >  2:3704766/4‑100 (MQ=255)

                      ttCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCa                                                                             <  1:3845100/100‑1 (MQ=255)

                        cAGGGAGATGACTGGAAGGCTCGTGAACCTCTGTGTCAGGCGTTGGCATATCGTGTGGAAGCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCTTg                                                                           <  2:620819/100‑1 (MQ=255)

                        cAGGGAGATAACTGGTAGGCTCGTGAGACATTGTGTCAGGTGTTGGCATAGAGCGTGCCATGTGGAGATTGGTGTGCTCGGCGAGCGGTCCTTAATCATg                                                                           >  2:360914/1‑100 (MQ=255)

                        cAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCCTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATg                                                                           >  2:848422/1‑100 (MQ=255)

                          gggAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGaa                                                                         <  1:2453865/100‑1 (MQ=255)

                           ggAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAAc                                                                        <  2:495148/100‑1 (MQ=255)

                              gaTAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAg                                                                     >  1:2986300/1‑100 (MQ=255)

                               aTAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGc                                                                    <  2:2284709/100‑1 (MQ=255)

                                  aCTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTcc                                                                 >  2:1714549/1‑100 (MQ=255)

                                   cTGGCAGTCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCg                                                                >  2:2564914/1‑100 (MQ=255)

                                   cTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGCCTGGTACGCGCTGCTAGCGGCCCTTACTCATGAACAACCTCCg                                                                >  1:3888468/1‑100 (MQ=255)

                                   cTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCg                                                                >  1:637517/1‑100 (MQ=255)

                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  2:3664018/1‑100 (MQ=255)

                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              <  1:2022512/100‑1 (MQ=255)

                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  1:1570660/1‑100 (MQ=255)

                                     ggCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGgc                                                              >  1:3388928/1‑100 (MQ=255)

                                        aGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGcgcg                                                           >  1:795271/1‑100 (MQ=255)

                                         ggCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGt                                                          <  2:803598/100‑1 (MQ=255)

                                          gCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGtt                                                         <  2:3550291/100‑1 (MQ=255)

                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttt                                                        <  2:3554968/100‑1 (MQ=255)

                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttt                                                        <  1:1346646/100‑1 (MQ=255)

                                           cTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGttn                                                        <  1:367528/100‑2 (MQ=255)

                                            tCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTa                                                       <  2:228460/100‑1 (MQ=255)

                                            tCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTa                                                       <  2:1239555/100‑1 (MQ=255)

                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     <  2:1216070/100‑1 (MQ=255)

                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  2:1355297/1‑100 (MQ=255)

                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  1:620654/1‑100 (MQ=255)

                                              gTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTaca                                                     >  1:1770102/1‑100 (MQ=255)

                                               tGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTacac                                                    <  2:778622/100‑1 (MQ=255)

                                               tGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTacac                                                    >  2:3685191/1‑100 (MQ=255)

                                                  aaCATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACActgg                                                 <  1:1526794/100‑1 (MQ=255)

                                                    cATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACActggct                                               >  1:1911138/1‑100 (MQ=255)

                                                       tgtgTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACtggctggc                                            <  2:2941751/100‑1 (MQ=255)

                                                       tgtgTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACtggctggc                                            <  1:1000331/100‑1 (MQ=255)

                                                        gtgtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCa                                           >  1:165093/1‑100 (MQ=255)

                                                          gtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAAc                                         <  2:584520/100‑1 (MQ=255)

                                                          gtCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAAc                                         <  1:3764965/100‑1 (MQ=255)

                                                            cAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCCACGt                                       >  1:1081226/1‑100 (MQ=255)

                                                              ggCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTg                                     >  2:2550511/1‑100 (MQ=255)

                                                               gCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGc                                    <  2:2733040/100‑1 (MQ=255)

                                                               gCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGc                                    <  1:181159/100‑1 (MQ=255)

                                                                 gTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACATGCTCCGGCTCGTTTACACTGGCTGGCAACGTTGCTg                                  <  2:457399/100‑1 (MQ=255)

                                                                 gTTGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTg                                  >  2:2902149/1‑100 (MQ=255)

                                                                   tGGCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgat                                <  1:2692792/100‑1 (MQ=255)

                                                                    ggCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatn                               <  1:1824805/100‑2 (MQ=255)

                                                                    ggCATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatg                               >  2:2026551/1‑100 (MQ=255)

                                                                      cATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatgga                             >  2:2376433/1‑100 (MQ=255)

                                                                      cATATAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatgga                             >  1:148270/1‑100 (MQ=255)

                                                                       atatAGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatggat                            <  2:553077/100‑1 (MQ=255)

                                                                        tataGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTgatggatg                           >  1:10015/1‑100 (MQ=255)

                                                                         ataGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgc                          <  2:143301/100‑1 (MQ=255)

                                                                          taGCGTGCCATCGGGCGACTGGTATGCGCTGCTGGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgcg                         <  2:252305/100‑1 (MQ=255)

                                                                          taGCGTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATgcg                         <  2:600081/100‑1 (MQ=255)

                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  1:1983269/1‑100 (MQ=255)

                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  2:1545654/1‑100 (MQ=255)

                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  2:151862/1‑100 (MQ=255)

                                                                              gTGCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCAGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaa                     >  1:182490/1‑100 (MQ=255)

                                                                                gCCATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTaaaa                   >  2:805499/1‑100 (MQ=255)

                                                                                  cATCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTCCACTGGCTGGCAACGTTGCTGATGGCTGCGCTAAAACg                 >  2:3159396/1‑100 (MQ=255)

                                                                                   aTCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGc                >  2:2553165/1‑100 (MQ=255)

                                                                                    tCGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGcc               >  1:1676599/1‑100 (MQ=255)

                                                                                     cGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCca              <  1:662694/100‑1 (MQ=255)

                                                                                     cGGGCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCca              <  1:28907/100‑1 (MQ=255)

                                                                                      gggCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCcat             >  1:541214/1‑100 (MQ=255)

                                                                                      gggCGACTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCcat             >  1:1442004/1‑100 (MQ=255)

                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  1:1379913/100‑1 (MQ=255)

                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  1:1237036/100‑1 (MQ=255)

                                                                                           aCTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATgg        <  2:3537918/100‑1 (MQ=255)

                                                                                            cTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGt       >  1:2465125/1‑100 (MQ=255)

                                                                                            cTGGTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGt       <  1:3634479/100‑1 (MQ=255)

                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  1:2043275/1‑100 (MQ=255)

                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  1:1668411/1‑100 (MQ=255)

                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     >  2:1131333/1‑100 (MQ=255)

                                                                                              ggTATGCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgc     <  2:2825391/100‑1 (MQ=255)

                                                                                                 aTTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCCACGTTGCTGATGGATGCGCTAAAACGCCATCATGGtgctgc  >  1:738770/1‑100 (MQ=255)

CCTGGCGCGGCACTGGCGTTGTTTCAGGGAGATAACTGGCAGGCTCGTGAAACATTGTGTCAGGCGTTGGCATATAGCGTGCCATCGGGCGACTGGTATTCGCTGCTAGCGGCCCTTAATCATGAACAAGCTCCGGCGCGTTTACACTGGCTGGCAACGTTGCTGATGGATGCGCTAAAACGCCATCATGGTGCTGC  >  NC_000913/1156353‑1156549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A