Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,294,845	A→C	pseudogene (148/567 nt)	insZ ←	pseudogene, transposase homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,294,845	0	A	C	95.6%	447.3 / 4.3	135	pseudogene (148/567 nt)	insZ	pseudogene, transposase homolog
Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (77/52); total (80/55)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 135 total aligned reads displayed.

CCAGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTAGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGCAAA  >  NC_000913/1294749‑1294941

ccAGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGc                                                                                               <  1:2793712/100‑1 (MQ=255)

ccAGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGc                                                                                               <  1:4042352/100‑1 (MQ=255)

ccAGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGc                                                                                               >  1:2924882/1‑100 (MQ=255)

  aGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGt                                                                                             <  1:3900632/100‑1 (MQ=255)

  aGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGt                                                                                             <  2:3592288/100‑1 (MQ=255)

    cacaGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTc                                                                                           <  2:2485716/100‑1 (MQ=255)

    cacaGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTc                                                                                           <  1:2366139/100‑1 (MQ=255)

     acaGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCt                                                                                          <  1:1301867/100‑1 (MQ=255)

      caGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTg                                                                                         >  1:2132881/1‑100 (MQ=255)

      caGTATGGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGGCTTCACAGGTTCATTAAGGACACCAGACGCATAACCGGGTCGGAGTTCTg                                                                                         >  2:3765871/1‑100 (MQ=255)

        gTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGcc                                                                                       >  1:1069635/1‑100 (MQ=255)

        gTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGcc                                                                                       >  1:138200/1‑100 (MQ=255)

        gTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGcc                                                                                       <  2:3929425/100‑1 (MQ=255)

         tttCGCTCTGCCGGTAAGGTGCGGTGACGGCATTCAGCAGAATGTGACTTCCCAGCTTCATCAAGGCCACCAGCCGCATAACCGGGTCGGCGTTCGGCCg                                                                                      >  2:3961210/1‑100 (MQ=255)

          ttCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCA‑TAGACGCATAACCGGGTCGGCGTTCTGCCGCt                                                                                    <  2:2155220/100‑1 (MQ=255)

           tCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCt                                                                                    <  1:3951/100‑1 (MQ=255)

              ctctGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAg                                                                                 >  1:1935687/1‑100 (MQ=255)

              ctctGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAg                                                                                 <  1:3433552/100‑1 (MQ=255)

               tctGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGt                                                                                <  2:446574/100‑1 (MQ=255)

               tctGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGt                                                                                <  2:44217/100‑1 (MQ=255)

               tctGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGt                                                                                <  1:1175672/100‑1 (MQ=255)

                 tGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTgg                                                                              <  1:2852133/100‑1 (MQ=255)

                 tGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTgg                                                                              <  2:1521455/100‑1 (MQ=255)

                  gCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGa                                                                             >  1:2865478/1‑100 (MQ=255)

                  gCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGa                                                                             >  2:3282494/1‑100 (MQ=255)

                   ccGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAt                                                                            <  1:87494/100‑1 (MQ=255)

                    cGGTAAGGTGCGGTCACGGCATTCATCAGAATGTGCCTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAtg                                                                           <  2:1107966/100‑1 (MQ=255)

                    cGGTAACGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAtg                                                                           <  1:3995491/100‑1 (MQ=255)

                     ggTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAtgt                                                                          >  1:2479860/1‑100 (MQ=255)

                     ggTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAtgt                                                                          <  2:1369028/100‑1 (MQ=255)

                     ggTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGAtgt                                                                          >  2:3451223/1‑100 (MQ=255)

                         aGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTg                                                                      <  1:325131/100‑1 (MQ=255)

                          ggTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTgg                                                                     >  1:2836864/1‑100 (MQ=255)

                          ggTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTgg                                                                     >  1:1561858/1‑100 (MQ=255)

                          ggTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTgg                                                                     >  2:1955001/1‑100 (MQ=255)

                           gTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGAAGCCTAACCGGGTAGGCGTTCTGCCGCTTAGTGGACGTGTTGGc                                                                    >  1:2031919/1‑100 (MQ=255)

                            tGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCa                                                                   <  1:92731/100‑1 (MQ=255)

                                ntCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGAcc                                                               >  1:3076952/2‑100 (MQ=255)

                                gTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCATTCTGCCGCTTAGTGGAGGTGTTGGCAGAcc                                                               >  2:3141496/1‑100 (MQ=255)

                                  cACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCa                                                             >  1:2539091/1‑100 (MQ=255)

                                        aTTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAatat                                                       >  1:138218/1‑100 (MQ=255)

                                        aTTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAatat                                                       >  1:3419628/1‑100 (MQ=255)

                                           cagcagAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCa                                                    >  2:1759781/1‑100 (MQ=255)

                                           cagcagAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCa                                                    >  1:1844097/1‑100 (MQ=255)

                                             gcagAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACg                                                  >  1:2567423/1‑100 (MQ=255)

                                             gcagAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTAGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACg                                                  >  1:1368702/1‑100 (MQ=255)

                                              cagAATGTGACTTCCCAGGTTCATTAAGGCCACCAGCCGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGc                                                 <  1:3845088/100‑1 (MQ=255)

                                               agAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCa                                                >  1:497260/1‑100 (MQ=255)

                                                gAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAg                                               >  1:896755/1‑100 (MQ=255)

                                                 aaTGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGc                                              <  1:4036353/100‑1 (MQ=255)

                                                   tgtgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTc                                            <  1:1099071/100‑1 (MQ=255)

                                                    gtgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCGGCTTAGTGGATGTGTTGGCAGACCCAGAATATTCACACAGATCg                                           >  1:3746624/1‑100 (MQ=255)

                                                    gtgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCg                                           >  1:2812263/1‑100 (MQ=255)

                                                     tgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACGGGGTAGGCG‑TCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCggg                                         <  2:2539032/100‑1 (MQ=255)

                                                     tgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCgg                                          <  1:321966/100‑1 (MQ=255)

                                                     tgACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCgg                                          >  2:3218294/1‑100 (MQ=255)

                                                      gACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCggg                                         <  1:421844/100‑1 (MQ=255)

                                                       aCTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGt                                        <  1:1462124/100‑1 (MQ=255)

                                                        cTTCCCAGTTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGtt                                       <  1:3436516/100‑1 (MQ=255)

                                                          tctcAGGTTCATTATGGCGACCCGACGCCTAACCGGGTCGGCCTTCTGCCGCTTAGTGGAGGTGTTGGCAGACCCATAATATTCATGCAGCTAGGGttca                                     >  2:846179/4‑98 (MQ=255)

                                                          tCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTa                                     >  1:1542648/1‑100 (MQ=255)

                                                             cAGGTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCa                                  >  1:3351487/1‑100 (MQ=255)

                                                               ggTTCATTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAgg                                <  1:3925609/100‑1 (MQ=255)

                                                                    aTTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTcc                           <  1:2071031/100‑1 (MQ=255)

                                                                    aTTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTcc                           >  2:758813/1‑100 (MQ=255)

                                                                    aTTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTcc                           >  1:2992523/1‑100 (MQ=255)

                                                                    aTTAAGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTcc                           >  1:610383/1‑100 (MQ=255)

                                                                        aGGCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGaa                       >  1:2219346/1‑100 (MQ=255)

                                                                         ggCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAAc                      <  1:1850320/100‑1 (MQ=255)

                                                                         ggCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAAc                      >  2:3886928/1‑100 (MQ=255)

                                                                         ggCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCACCTCGGGTTTATCAGGTGTCCTGAAc                      >  2:2177595/1‑100 (MQ=255)

                                                                          gCCACCAGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGCTGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACt                     >  2:3901272/1‑100 (MQ=255)

                                                                          gCCACCAGACGCATAACCGGGTAGGCGTTCTGCCGATTAGTGGATGTGTTGGCAGAACCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACt                     <  2:389783/100‑1 (MQ=255)

                                                                           ccaccaGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACtg                    >  2:956760/1‑100 (MQ=255)

                                                                           ccaccaGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACtg                    >  1:28305/1‑100 (MQ=255)

                                                                            caccaGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAAcacg                   >  2:2777073/1‑97 (MQ=255)

                                                                             accaGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACtgtg                  >  1:1219152/1‑100 (MQ=255)

                                                                             accaGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACtgtg                  <  2:1815078/100‑1 (MQ=255)

                                                                              ccaGGCGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTgc                 >  2:2380012/1‑100 (MQ=255)

                                                                               caGACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTgcg                >  1:234257/1‑100 (MQ=255)

                                                                                 gACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGcc              >  2:1476976/1‑100 (MQ=255)

                                                                                 gACGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGcc              >  1:3716359/1‑100 (MQ=255)

                                                                                   cGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCAt            >  1:1132686/1‑100 (MQ=255)

                                                                                   cGCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCAt            >  1:3347544/1‑100 (MQ=255)

                                                                                    gCATAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGGGTCCTGAACTGTGCGCCATc           <  1:3100628/100‑1 (MQ=255)

                                                                                      aTAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCaa         >  1:3715657/1‑100 (MQ=255)

                                                                                      aTAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCaa         >  1:2807336/1‑100 (MQ=255)

                                                                                      aTAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCaa         >  2:2931826/1‑100 (MQ=255)

                                                                                       tAACCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAAt        >  1:2252107/1‑100 (MQ=255)

                                                                                        aaCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTTGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATg       <  1:3846763/100‑1 (MQ=255)

                                                                                        aaCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATg       <  1:1740524/100‑1 (MQ=255)

                                                                                         aCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATgg      >  1:2916585/1‑100 (MQ=255)

                                                                                         aCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATgg      >  2:775008/1‑100 (MQ=255)

                                                                                         aCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATgg      >  2:886379/1‑100 (MQ=255)

                                                                                         aCCGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTCGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATgg      <  1:331929/100‑1 (MQ=255)

                                                                                          ccGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGGCCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGc     <  2:557763/100‑1 (MQ=255)

                                                                                          ccGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGc     <  2:1110312/100‑1 (MQ=255)

                                                                                          ccGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGAGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGc     <  2:2071795/100‑1 (MQ=255)

                                                                                           cGGGTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGCa    >  2:3452714/1‑100 (MQ=255)

                                                                                             ggTCGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGCaaa  >  2:2118645/1‑100 (MQ=255)

CCAGCACAGTTTCGCTCTGCCGGTAAGGTGCGGTCACGGCATTCAGCAGAATGTGACTTCCCAGGTTCATTAAGGCCACCAGACGCATAACCGGGTAGGCGTTCTGCCGCTTAGTGGATGTGTTGGCAGACCCATAATATTCACGCAGCTCGGGTTTATCAGGTGTCCTGAACTGTGCGCCATCAATGGCAAA  >  NC_000913/1294749‑1294941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A