Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,520	A→C	N184K (AAT→AAG)	ydcI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,520	0	A	C	94.8%	486.1 / 11.0	153	N184K (AAT→AAG)	ydcI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (1/7); new base C (63/82); total (64/89)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 155 total aligned reads displayed.

TGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCG  >  NC_000913/1494421‑1494619

tGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGa                                                                                                     <  1:3081892/100‑1 (MQ=255)

 gCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGAt                                                                                                    <  1:3330423/100‑1 (MQ=255)

  cATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCtt                                                                                                   >  2:2906722/1‑100 (MQ=255)

  cATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGAtt                                                                                                   <  2:2555035/100‑1 (MQ=255)

  cATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGAGTGCtt                                                                                                   >  1:2112274/1‑100 (MQ=255)

   aTCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTGCTCCTGAAGTAGCGGGTGCTTa                                                                                                  <  1:3412675/100‑1 (MQ=255)

    tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAg                                                                                                 <  2:2372194/100‑1 (MQ=255)

    tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAg                                                                                                 <  2:2390511/100‑1 (MQ=255)

    tCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAg                                                                                                 <  1:1639982/100‑1 (MQ=255)

      tGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAggg                                                                                               <  1:427759/100‑1 (MQ=255)

      tGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAggg                                                                                               <  1:3522409/100‑1 (MQ=255)

       gAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGc                                                                                              <  1:1091565/100‑1 (MQ=255)

       gAATGCTGGCGGGGCGCAGTACCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGc                                                                                              <  2:1857750/100‑1 (MQ=255)

        aaTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCg                                                                                             <  1:3681052/100‑1 (MQ=255)

         aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGCCCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCgg                                                                                            <  1:2670395/100‑1 (MQ=255)

         aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCgg                                                                                            >  2:2990728/1‑100 (MQ=255)

         aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCgg                                                                                            >  2:319807/1‑100 (MQ=255)

         aTGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCgg                                                                                            <  1:639615/100‑1 (MQ=255)

          tGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGa                                                                                           >  2:1719958/1‑100 (MQ=255)

            cTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACa                                                                                         <  2:201782/100‑1 (MQ=255)

            cTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCGTAGCGTTACGTTCTCCTGAAGTAGCGGTTGCTTAGTTCGGACa                                                                                         >  1:1206385/1‑100 (MQ=255)

              ggcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTTAAGTAGCGGGTGCTTAGGGCGGACacc                                                                                       >  2:3537000/1‑98 (MQ=255)

              ggcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAAc                                                                                       >  2:232982/1‑100 (MQ=255)

               gcgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAAcc                                                                                      <  2:2316751/100‑1 (MQ=255)

                cgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCa                                                                                     >  2:2911710/1‑100 (MQ=255)

                cgtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCa                                                                                     <  2:3351450/100‑1 (MQ=255)

                 gtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAg                                                                                    <  1:1777226/100‑1 (MQ=255)

                 gtggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAg                                                                                    <  2:1878871/100‑1 (MQ=255)

                   ggcgCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCt                                                                                  <  2:1635432/100‑1 (MQ=255)

                    gcgcAGTGCCTTATGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTAAGTTCTCATGAAGTAGCGGGTGCTTAGAGAGGACACCCAGCtt                                                                                 >  1:2030186/1‑100 (MQ=255)

                     cgcAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTc                                                                                >  1:1518420/1‑100 (MQ=255)

                      gcAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCa                                                                               <  1:1407664/100‑1 (MQ=255)

                        aGTGCCTTCTGGTGATACAACGACCGGGCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAAc                                                                             <  2:526173/100‑1 (MQ=255)

                        aGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAAc                                                                             <  1:1694448/100‑1 (MQ=255)

                        aGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAAc                                                                             <  2:523343/100‑1 (MQ=255)

                         gTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACg                                                                            >  1:2218678/1‑100 (MQ=255)

                            ccTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCCGCTTCAACGAtt                                                                         <  2:3501354/100‑1 (MQ=255)

                            ccTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGAtt                                                                         <  1:251634/100‑1 (MQ=255)

                                cTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAg                                                                     <  1:3069307/100‑1 (MQ=255)

                                cTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAg                                                                     <  1:236774/100‑1 (MQ=255)

                                cTGGTGATACAACGACCGGCCATTCCAGCACCCGGATTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTAAACGATTCAAg                                                                     <  2:785035/100‑1 (MQ=255)

                                 tGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGa                                                                    >  1:1941807/1‑100 (MQ=255)

                                  ggTGATACATCGACCGGCCATTCCAGCACCCGTCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGaa                                                                   <  2:3460761/100‑1 (MQ=255)

                                  ggTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGaa                                                                   >  1:2841430/1‑100 (MQ=255)

                                  ggTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGaa                                                                   >  1:3992514/1‑100 (MQ=255)

                                  ggTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGaa                                                                   >  2:246953/1‑100 (MQ=255)

                                   gTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGaaa                                                                  >  1:2990155/1‑100 (MQ=255)

                                    gtaTACAACGACCGGCCATTCCAACGCCAGGCTTAACGTTACCTACACCTGAACTAGCGGGTTCTTAGAACGTACAACCACCTTCAACGATTCAAAAAAc                                                                 <  2:836571/98‑1 (MQ=255)

                                     gATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAca                                                                >  2:304309/1‑100 (MQ=255)

                                         cAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAcagcag                                                            >  1:3866011/1‑100 (MQ=255)

                                         cAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAcagcag                                                            >  2:2675006/1‑100 (MQ=255)

                                         cAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAAcagcag                                                            >  1:177466/1‑100 (MQ=255)

                                          aaCGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGt                                                           >  1:1532379/1‑100 (MQ=255)

                                           aCGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCAGACAACCAGCTTCAACGATTCAAGAAACAGCAGtt                                                          >  2:3949243/1‑100 (MQ=255)

                                             gACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCg                                                        >  2:675518/1‑100 (MQ=255)

                                                cGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTaa                                                     >  2:2695290/1‑100 (MQ=255)

                                                 tgCCAGTCCAGCACCCGGCTTAGCGTTACGCCCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAt                                                    <  2:2239808/99‑1 (MQ=255)

                                                 ggCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAAt                                                    <  1:1656/100‑1 (MQ=255)

                                                   ccATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTa                                                  <  2:2437320/100‑1 (MQ=255)

                                                   ccATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTa                                                  <  2:3932754/100‑1 (MQ=255)

                                                    cATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTaa                                                 >  2:2791873/1‑100 (MQ=255)

                                                     aTTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAg                                                >  2:3744979/1‑100 (MQ=255)

                                                     aTTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAg                                                <  2:107291/100‑1 (MQ=255)

                                                       tCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                              <  1:3035813/100‑1 (MQ=255)

                                                       tCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGcc                                              >  1:2448212/1‑100 (MQ=255)

                                                        ccAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGccc                                             >  2:2262567/1‑100 (MQ=255)

                                                         cAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCg                                            >  1:3000954/1‑100 (MQ=255)

                                                          aGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCgg                                           <  1:1155836/100‑1 (MQ=255)

                                                          aGCACCCGGCTTAGCGCTACGTTCTCCTGGAGTTGCGGTTGCTTAGGGCGGACAACCAGCTTGAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCgg                                           <  2:1073630/100‑1 (MQ=255)

                                                           tctcccGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGt                                          <  2:3692129/97‑1 (MQ=255)

                                                              cccGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcat                                       >  1:2549572/1‑100 (MQ=255)

                                                              cccGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcat                                       >  1:2563313/1‑100 (MQ=255)

                                                              cccGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcat                                       >  2:2740908/1‑100 (MQ=255)

                                                                cGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcatca                                     <  1:2427388/100‑1 (MQ=255)

                                                                cGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcatca                                     <  2:2086856/100‑1 (MQ=255)

                                                                cGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcatca                                     >  1:3930828/1‑100 (MQ=255)

                                                                     tAGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCAGCAGTTCa                                <  1:3317841/100‑1 (MQ=255)

                                                                     tAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCa                                >  1:1209674/1‑100 (MQ=255)

                                                                      aGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAg                               <  2:969936/100‑1 (MQ=255)

                                                                      aGCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAg                               <  2:1492287/100‑1 (MQ=255)

                                                                       gCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAgg                              <  2:3919788/100‑1 (MQ=255)

                                                                       gCGTTACGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAgg                              <  1:50129/100‑1 (MQ=255)

                                                                        cGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGa                             <  2:2571584/100‑1 (MQ=255)

                                                                             cGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGa                        <  2:1458161/100‑1 (MQ=255)

                                                                             cGTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGa                        >  1:787306/1‑100 (MQ=255)

                                                                              gTTCTCCTGAAGTAGCGGTGGCTTAGGGCGGAGAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTTATCATTTCAGGATTTGAc                       <  2:3387806/100‑1 (MQ=255)

                                                                              gTTCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGAc                       <  2:2798583/100‑1 (MQ=255)

                                                                               ttCTCCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACa                      <  1:4048544/100‑1 (MQ=255)

                                                                                 ctcCTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATc                    >  2:3535693/1‑100 (MQ=255)

                                                                                 ctcCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATc                    <  2:1186660/100‑1 (MQ=255)

                                                                                   ccTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCg                  >  2:1959083/1‑100 (MQ=255)

                                                                                   ccTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCg                  >  1:739383/1‑100 (MQ=255)

                                                                                   ccTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTAAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCg                  >  2:425082/1‑100 (MQ=255)

                                                                                    cTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGa                 >  1:1369818/1‑100 (MQ=255)

                                                                                    cTGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGa                 <  2:3859555/100‑1 (MQ=255)

                                                                                     tGAAGTAGCGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTTAGGATCTGACATCCGAc                >  1:362069/1‑100 (MQ=255)

                                                                                             cGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCg        <  1:3724503/100‑1 (MQ=255)

                                                                                             cGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCg        <  1:3724276/100‑1 (MQ=255)

                                                                                             cGGGTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCg        <  1:1990390/100‑1 (MQ=255)

                                                                                                gTGCTTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTGATCAGTTCAGCATCAGACATCCGACCAATGCCGata     >  2:3789334/1‑100 (MQ=255)

TGCATCTGAATGCTGGCGTGGCGCAGTGCCTTCTGGTGATACAACGACCGGCCATTCCAGCACCCGGCTTAGCGTTACGTTCTCCTGAAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCG  >  NC_000913/1494421‑1494619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A