Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,815,451	T→G	F529V (TTT→GTT)	katE →	catalase HPII, heme d‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,815,451	0	T	G	99.2%	413.0 / ‑5.0	117	F529V (TTT→GTT)	katE	catalase HPII, heme d‑containing
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (55/61); total (55/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 118 total aligned reads displayed.

GCGCAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGAAA  >  NC_000913/1815354‑1815546

gcgcAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGtgtt                                                                                               >  1:507126/1‑100 (MQ=255)

 cgcAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTg                                                                                              <  1:1066986/100‑1 (MQ=255)

 cgcAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTg                                                                                              <  2:3862821/100‑1 (MQ=255)

 cgcAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTg                                                                                              <  1:3918876/100‑1 (MQ=255)

 cgcAGCCCATCCTTCGGCGACTATTATTCCCACGCGCGTATGTTCTGGCTAAGTCAGTCGCCATTTGAGCAGCGCAATATTGTCGATTGTTTCAGTGTTg                                                                                              <  2:246109/100‑1 (MQ=255)

   cAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCCGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAg                                                                                            <  1:3937913/100‑1 (MQ=255)

     gCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGtt                                                                                          >  2:2576142/1‑100 (MQ=255)

     gCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGtt                                                                                          >  1:1316808/1‑100 (MQ=255)

     gCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGtt                                                                                          <  1:2815475/100‑1 (MQ=255)

      cccATCCTTTGGCGAATATTACTCCCATCCGCGTCTGTTCTGGCTAAGTCACACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTa                                                                                         <  1:241699/100‑1 (MQ=255)

        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                       <  1:702928/100‑1 (MQ=255)

        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                       <  2:2930021/100‑1 (MQ=255)

        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                       <  1:638124/100‑1 (MQ=255)

        cATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAg                                                                                       <  1:443031/100‑1 (MQ=255)

           cGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCaa                                                                                    <  1:195013/100‑1 (MQ=255)

             tttGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCGGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAg                                                                                  <  1:1544243/100‑1 (MQ=255)

              ttGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGt                                                                                 <  1:1210531/100‑1 (MQ=255)

              ttGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGt                                                                                 <  1:337626/100‑1 (MQ=255)

               tGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtc                                                                                >  2:3220283/1‑100 (MQ=255)

                ggCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcg                                                                               >  2:2559789/1‑100 (MQ=255)

                ggCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTAAGTGTTGCGTTAAGCCAAGtag                                                                               >  1:1009672/1‑98 (MQ=255)

                 gCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgt                                                                              >  1:3285875/1‑100 (MQ=255)

                  cGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgtt                                                                             <  2:15528/100‑1 (MQ=255)

                  cGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgtt                                                                             >  2:3782985/1‑100 (MQ=255)

                   gAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttc                                                                            <  2:2826425/100‑1 (MQ=255)

                   gAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttc                                                                            >  2:2628822/1‑100 (MQ=255)

                   gAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttc                                                                            <  1:3935117/100‑1 (MQ=255)

                   gAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGtcgttc                                                                            >  1:3001616/1‑100 (MQ=255)

                       attattCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTcc                                                                        <  2:12707/100‑1 (MQ=255)

                        ttattCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCg                                                                       >  1:1440712/1‑100 (MQ=255)

                         tattCCCATCCGCGTCTGTTCTGGCAAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGACGTTCGTCCGt                                                                      >  2:3189255/1‑100 (MQ=255)

                           ttCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGtat                                                                    <  1:1573392/100‑1 (MQ=255)

                           ttCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGtat                                                                    <  2:1906376/100‑1 (MQ=255)

                           ttCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGtat                                                                    >  1:3529079/1‑100 (MQ=255)

                              ccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATAtt                                                                 >  2:616172/1‑100 (MQ=255)

                               cATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTc                                                                >  2:1161997/1‑100 (MQ=255)

                                aTCCGCGTCTGTTCTGGCTAAGTCAGACTCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCg                                                               <  1:2547605/100‑1 (MQ=255)

                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                             <  2:2837337/100‑1 (MQ=255)

                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                             >  1:1553906/1‑100 (MQ=255)

                                  ccGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTg                                                             >  1:2581840/1‑100 (MQ=255)

                                   cgcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGa                                                            >  1:211798/1‑100 (MQ=255)

                                   cgcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGa                                                            >  2:1569452/1‑100 (MQ=255)

                                   cgcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGa                                                            <  1:2829484/100‑1 (MQ=255)

                                    gcgTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAg                                                           <  1:1084428/100‑1 (MQ=255)

                                        cTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCTTATTGTCCATGGTTTCAGTGATGAGTTAAGCAAAGTCGTTCGTCCGTATATTAGTGAGcgcg                                                       >  2:3908081/1‑100 (MQ=255)

                                        cTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGcgcg                                                       <  2:379993/100‑1 (MQ=255)

                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                     >  1:3994352/1‑100 (MQ=255)

                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                     <  2:1963007/100‑1 (MQ=255)

                                          gTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgtt                                                     <  1:3078721/100‑1 (MQ=255)

                                           ttCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttg                                                    <  2:2820210/100‑1 (MQ=255)

                                           ttCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttg                                                    >  1:929161/1‑100 (MQ=255)

                                           ttCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttg                                                    <  1:152719/100‑1 (MQ=255)

                                           ttCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttg                                                    <  1:3108359/100‑1 (MQ=255)

                                            tCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCgttgt                                                   <  2:648168/100‑1 (MQ=255)

                                              tGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATCGTCGATGGTTTCACTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGttgttg                                                 <  2:3941865/100‑1 (MQ=255)

                                               ggCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGa                                                >  2:2172648/1‑100 (MQ=255)

                                                 cTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCACAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc                                              >  2:495346/1‑100 (MQ=255)

                                                 cTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc                                              >  2:3853410/1‑100 (MQ=255)

                                                     gTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt                                          >  2:2162803/1‑100 (MQ=255)

                                                     gTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt                                          >  1:3389013/1‑100 (MQ=255)

                                                     gTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt                                          >  2:1050505/1‑100 (MQ=255)

                                                      tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg                                         >  2:1710134/1‑100 (MQ=255)

                                                        aGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgc                                       >  2:2765112/1‑100 (MQ=255)

                                                         gACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgcg                                      >  2:1887504/1‑100 (MQ=255)

                                                         gACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgcg                                      <  1:1854013/100‑1 (MQ=255)

                                                         gACGCCATTTGAGCAGCGCCATATAGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGgcg                                      <  2:3102788/100‑1 (MQ=255)

                                                           cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCa                                    <  1:2594510/100‑1 (MQ=255)

                                                               aTTTGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATAtt                                <  1:2889692/100‑1 (MQ=255)

                                                                 ttGAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGa                              >  1:2184772/1‑100 (MQ=255)

                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                            <  2:2236406/100‑1 (MQ=255)

                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                            >  2:2296626/1‑100 (MQ=255)

                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                            <  2:2985183/100‑1 (MQ=255)

                                                                   gAGCAGCGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTGCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtc                            <  1:1621601/100‑1 (MQ=255)

                                                                     gcagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtctc                          <  1:2418333/100‑1 (MQ=255)

                                                                     gcagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtctc                          >  2:3771646/1‑100 (MQ=255)

                                                                     gcagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtctc                          <  2:3194314/100‑1 (MQ=255)

                                                                      cagcGCCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCa                         <  1:2599503/100‑1 (MQ=255)

                                                                         cgcCATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCActc                      <  2:2084684/100‑1 (MQ=255)

                                                                           ccATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTg                    >  1:1572892/1‑100 (MQ=255)

                                                                           ccATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTg                    >  2:2584708/1‑100 (MQ=255)

                                                                           ccATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTg                    <  1:721705/100‑1 (MQ=255)

                                                                            cATATTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTgg                   >  1:3349850/1‑100 (MQ=255)

                                                                              tatTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGcc                 <  2:1303929/100‑1 (MQ=255)

                                                                              tatTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGcc                 >  2:2769830/1‑100 (MQ=255)

                                                                              tatTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGcc                 >  1:2200344/1‑100 (MQ=255)

                                                                               atTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGccc                <  1:1080015/100‑1 (MQ=255)

                                                                               atTGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGccc                >  1:571706/1‑100 (MQ=255)

                                                                                ttGTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa               <  1:1370766/100‑1 (MQ=255)

                                                                                 tGTCGCTGGTTTCAGTGTTGAGTTACGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAg              <  2:1992561/100‑1 (MQ=255)

                                                                                  gTCGATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAgg             >  2:1530969/1‑100 (MQ=255)

                                                                                  gTCGATGGTTTCAGTGGTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGAGGTTGACCAGCTGGCGCATATTGATCTCACGCTGGCCGAgg             >  2:1866990/1‑100 (MQ=255)

                                                                                     gATGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCgg          <  1:612203/100‑1 (MQ=255)

                                                                                       tGGTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTg        <  1:2222761/100‑1 (MQ=255)

                                                                                        ggTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTgg       >  1:3955270/1‑100 (MQ=255)

                                                                                        ggTTTCAGTGTGGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTgg       <  2:1100970/100‑1 (MQ=255)

                                                                                         gTTTCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGc      <  1:334099/100‑1 (MQ=255)

                                                                                           ttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGa    >  2:3855630/1‑100 (MQ=255)

                                                                                           ttCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGa    >  2:2144823/1‑100 (MQ=255)

                                                                                            tCAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGaa   >  1:1921789/1‑100 (MQ=255)

                                                                                             cAGTGTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGaaa  <  1:2691036/100‑1 (MQ=255)

GCGCAGCCCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCAGGCGGTGGCGAAA  >  NC_000913/1815354‑1815546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A