Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,822,043 T→G I72L (ATC→CTC)  osmE ← osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,822,0430TG97.6% 428.7 / ‑1.9 128I72L (ATC→CTC) osmEosmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (66/58);  total (67/61)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTT  >  NC_000913/1821946‑1822138
                                                                                                 |                                                                                               
tATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGt                                                                                               <  1:2116764/100‑1 (MQ=255)
tATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGt                                                                                               <  1:2071328/100‑1 (MQ=255)
 aTTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAACGTAGGTTTCTGCTTTACCGTCACGTTGACCCAGGAGGTa                                                                                              >  1:3822766/1‑100 (MQ=255)
    cAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGaggtaggt                                                                                           >  1:111523/1‑100 (MQ=255)
     aGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTc                                                                                          >  1:1112654/1‑100 (MQ=255)
      gCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCt                                                                                         >  1:4081390/1‑100 (MQ=255)
       cacaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTg                                                                                        <  2:2913099/100‑1 (MQ=255)
       cacaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTg                                                                                        <  2:408962/100‑1 (MQ=255)
       cacaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTg                                                                                        >  1:2236777/1‑100 (MQ=255)
       cacaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTg                                                                                        >  1:1698328/1‑100 (MQ=255)
        acaGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTgg                                                                                       <  1:2716999/100‑1 (MQ=255)
        acaGGTCTGAGAACCGGAGTTGATGACATGTCCGGTAGCAGCTAACGCGACAAAGTAGGTTGCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTgg                                                                                       <  2:1997512/100‑1 (MQ=255)
         caGTTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                      <  2:21831/100‑1 (MQ=255)
         caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                      >  1:2675144/1‑100 (MQ=255)
         caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                      <  1:6582/100‑1 (MQ=255)
         caGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGc                                                                                      <  2:1148789/100‑1 (MQ=255)
          aGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCa                                                                                     <  1:1252579/100‑1 (MQ=255)
           ggTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCaa                                                                                    >  1:3466743/1‑100 (MQ=255)
               tGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAc                                                                                >  2:116304/1‑100 (MQ=255)
               tGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAc                                                                                >  1:2005119/1‑100 (MQ=255)
                gATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAcc                                                                               >  2:3497624/1‑100 (MQ=255)
                gATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTAcc                                                                               <  1:2883233/100‑1 (MQ=255)
                  tagcgggAGTTGAGGACATGTCCGGTATGATCTAACGCGACAAAGTAGGGTTCTGCTTTTCCATCGCGTTGTCCCATGAGGTAGGTCTGGCAAGTACcgc                                                                             <  2:2416700/95‑1 (MQ=255)
                  tAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcgc                                                                             <  2:686979/100‑1 (MQ=255)
                   aaCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACcgcg                                                                            >  1:3491916/1‑100 (MQ=255)
                    aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGa                                                                           >  2:273387/1‑100 (MQ=255)
                    aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGa                                                                           >  2:2425637/1‑100 (MQ=255)
                    aCCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGa                                                                           <  1:396919/100‑1 (MQ=255)
                     ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAg                                                                          >  1:1531354/1‑100 (MQ=255)
                     ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAg                                                                          >  2:1243779/1‑100 (MQ=255)
                     ccGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGAAGGTTTCTGCTTTACCATCACATTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAg                                                                          >  1:1987987/1‑100 (MQ=255)
                      cGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGc                                                                         >  1:1306736/1‑100 (MQ=255)
                      cGGAGTTGATGACATGGCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCCTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGc                                                                         <  2:710721/100‑1 (MQ=255)
                         aGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATg                                                                      <  1:256510/100‑1 (MQ=255)
                          gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATgn                                                                     <  1:1747794/100‑2 (MQ=255)
                          gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                     >  1:2351887/1‑100 (MQ=255)
                          gTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATgg                                                                     <  2:3243625/100‑1 (MQ=255)
                             gatgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTATACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATc                                                                  >  1:523672/1‑100 (MQ=255)
                              atgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCa                                                                 >  2:2597177/1‑100 (MQ=255)
                               tgaCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCAt                                                                >  2:2328264/1‑100 (MQ=255)
                                 aCATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGc                                                              <  1:961896/100‑1 (MQ=255)
                                    tGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCa                                                           <  1:3002440/100‑1 (MQ=255)
                                    tGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTCGCAAGTCCCGCGAGCATGGATCATGCTCa                                                           >  1:3720252/1‑100 (MQ=255)
                                     gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                          >  1:2961140/1‑100 (MQ=255)
                                     gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                          >  1:33459/1‑100 (MQ=255)
                                     gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCAc                                                          <  1:723966/100‑1 (MQ=255)
                                     gTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGATCCATGCTcgn                                                          <  2:3149198/100‑3 (MQ=255)
                                      tCCGGTATCATCTAACGCGACACAGTAGGTTTCTGCTCTACCATCACGTTGACCCAGGATGTAGGTCGGGCAAGTACGGCGCGTATGATCCATGCTcgcg                                                         <  2:1620935/100‑4 (MQ=255)
                                        cGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGCGCATGGATCATGCTCACTTc                                                       <  1:4055750/100‑1 (MQ=255)
                                         gttATGATCGAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                      <  2:1167476/98‑1 (MQ=255)
                                         ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                      >  2:3158656/1‑100 (MQ=255)
                                         ggTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCa                                                      <  1:2124162/100‑1 (MQ=255)
                                          gTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAg                                                     <  2:1092581/100‑1 (MQ=255)
                                              catcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGa                                                 >  2:432279/1‑100 (MQ=255)
                                               atcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGaa                                                >  1:471973/1‑100 (MQ=255)
                                                tcTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAg                                               <  1:3777899/100‑1 (MQ=255)
                                                 cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                              <  2:3709106/100‑1 (MQ=255)
                                                 cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                              <  1:2610734/100‑1 (MQ=255)
                                                 cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                              >  1:2441230/1‑100 (MQ=255)
                                                 cTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAgg                                              <  1:1463803/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                            <  2:1504070/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                            <  1:739590/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGtt                                            <  2:3079505/100‑1 (MQ=255)
                                                   aaCGCGACAAAGTAAGTTTCTGCTTTACCATCACGTTGACCCAGGAGATAGGTCAGGCAAGTACCGCGAGCATGGATCATGCTTACATCAGACGTAGGtg                                            >  2:3732212/1‑99 (MQ=255)
                                                     cgcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCGAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTa                                          >  1:1802832/1‑100 (MQ=255)
                                                       cgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAcc                                        <  2:2662983/100‑1 (MQ=255)
                                                        gACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAccc                                       <  2:2251937/100‑1 (MQ=255)
                                                         aCAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                      <  2:1973675/100‑1 (MQ=255)
                                                         aCAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCg                                      <  1:1332638/100‑1 (MQ=255)
                                                          cAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGc                                     >  1:2864884/1‑100 (MQ=255)
                                                          cAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGc                                     <  1:3154973/100‑1 (MQ=255)
                                                           aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCa                                    <  1:2306560/100‑1 (MQ=255)
                                                           aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCa                                    <  1:713098/100‑1 (MQ=255)
                                                            aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACGCGCaa                                   >  1:3864417/1‑100 (MQ=255)
                                                                 ggTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt                              >  1:2835033/1‑100 (MQ=255)
                                                                 ggTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt                              <  1:2247957/100‑1 (MQ=255)
                                                                 ggTTTCTACTTCACAGTCGCGTTGACCCAGGTGGTAGGTGTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACAAAGGTTTACCCGCAATTtgt                              <  2:3277073/100‑1 (MQ=255)
                                                                  gTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                             >  1:2333853/1‑100 (MQ=255)
                                                                  gTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgtg                             <  1:3324388/100‑1 (MQ=255)
                                                                  gTTTCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCACAATTtgtg                             <  1:3978770/100‑1 (MQ=255)
                                                                   tttCTGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGc                            >  1:3526641/1‑100 (MQ=255)
                                                                       tGCTTTACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAcc                        >  1:721193/1‑100 (MQ=255)
                                                                            tACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcgc                   <  1:810057/100‑1 (MQ=255)
                                                                            tACCATCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcgc                   >  1:1004668/1‑100 (MQ=255)
                                                                              ccATCACGTTGCCCCAGAAGGCAGGTCTGGCAAGAACCGCCAGCATGGATCATGCTCACTTCAGACCAAGGTCTACCCGCAATTTGTGCAACCTGCGccc                 >  2:88391/1‑100 (MQ=255)
                                                                                aTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCgg               >  2:1801522/1‑100 (MQ=255)
                                                                                aTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCgg               >  1:60504/1‑100 (MQ=255)
                                                                                aTCACGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCgg               <  1:1042236/100‑1 (MQ=255)
                                                                                 tCACGTTGACCCAGGAGGTAGGTATGGAAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCAGGc              >  2:1376626/1‑100 (MQ=255)
                                                                                   aCGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc            >  2:1430786/1‑100 (MQ=255)
                                                                                   aCGTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc            >  1:1517360/1‑100 (MQ=255)
                                                                                     gTTGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCAt          <  2:1505097/100‑1 (MQ=255)
                                                                                       tGACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGc        >  1:3062370/1‑100 (MQ=255)
                                                                                        gACCCAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGcc       <  2:2517997/100‑1 (MQ=255)
                                                                                          cccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtt     >  2:2086795/1‑100 (MQ=255)
                                                                                          cccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCtt     >  1:609917/1‑100 (MQ=255)
                                                                                           ccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttt    >  2:512486/1‑100 (MQ=255)
                                                                                           ccAGGAGGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttt    >  1:1356066/1‑100 (MQ=255)
                                                                                             aggaggTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttttt  <  2:3944371/100‑1 (MQ=255)
                                                                                             aggaggTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCttttt  <  1:1269970/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTCATGCCTTTTT  >  NC_000913/1821946‑1822138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A