Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,019,670 T→G I18S (ATT→AGT)  fliL → flagellar biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,6700TG99.2% 406.5 / NA 121I18S (ATT→AGT) fliLflagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (59/61);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 121 total aligned reads displayed.

GATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGT  >  NC_000913/2019573‑2019766
                                                                                                 |                                                                                                
gATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTc                                                                                                <  1:745837/100‑1 (MQ=255)
 aTAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCt                                                                                               >  2:3533382/1‑100 (MQ=255)
  tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGCTTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTAGATCCCGAGTCTg                                                                                              >  1:3679115/1‑100 (MQ=255)
  tAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTg                                                                                              <  1:3503273/100‑1 (MQ=255)
     ttAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTa                                                                                           <  1:366548/100‑1 (MQ=255)
     ttAGCCGATAAGCAGTAGCGACACAGGAAGACAGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTa                                                                                           <  2:1248500/100‑1 (MQ=255)
      tAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTAt                                                                                          >  1:1859178/1‑100 (MQ=255)
      tAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTAt                                                                                          <  1:325619/100‑1 (MQ=255)
         ccGATAAGCAGTAGCGACACAGGAAGACCGCACCACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCa                                                                                       <  1:190110/100‑1 (MQ=255)
         ccGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCa                                                                                       <  1:2554186/100‑1 (MQ=255)
         ccGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCa                                                                                       <  2:2836317/100‑1 (MQ=255)
         ccGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCa                                                                                       >  1:2330792/1‑100 (MQ=255)
          cGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCAt                                                                                      >  2:2276994/1‑100 (MQ=255)
            tctaGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTGTGGTATTCATTa                                                                                    <  1:3510342/97‑1 (MQ=255)
             tAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCTATAAGCAAGAAAAGCAAGCGATCGCTTTGGAGCCCGAGTCTGGTATTCATTAc                                                                                   <  2:2764828/100‑1 (MQ=255)
             tAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAc                                                                                   <  1:1188966/100‑1 (MQ=255)
             tAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAc                                                                                   <  1:2815570/100‑1 (MQ=255)
               aGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTaaga                                                                                 >  2:3435275/1‑97 (MQ=255)
               aGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAccc                                                                                 <  2:3851194/100‑1 (MQ=255)
               aGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAccc                                                                                 >  1:1437711/1‑100 (MQ=255)
               aGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAccc                                                                                 <  1:653109/100‑1 (MQ=255)
               aGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTAccc                                                                                 >  1:1830262/1‑100 (MQ=255)
                gCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCt                                                                                >  2:1229870/1‑100 (MQ=255)
                gCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCt                                                                                >  1:3779871/1‑100 (MQ=255)
                   gTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTcgc                                                                             <  2:288703/100‑1 (MQ=255)
                   gTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTcgc                                                                             >  1:2140830/1‑100 (MQ=255)
                    tAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTcgcg                                                                            >  2:131111/1‑100 (MQ=255)
                        gACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCt                                                                        >  1:2930895/1‑100 (MQ=255)
                         acacAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtg                                                                       <  2:121364/100‑1 (MQ=255)
                         acacAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtg                                                                       <  2:1202186/100‑1 (MQ=255)
                         acacAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtg                                                                       <  1:3503418/100‑1 (MQ=255)
                         acacAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtg                                                                       <  1:2268550/100‑1 (MQ=255)
                         acacAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtg                                                                       <  2:1600153/100‑1 (MQ=255)
                          cacaGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtgt                                                                      >  2:2747930/1‑100 (MQ=255)
                          cacaGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCtgt                                                                      >  1:3483701/1‑100 (MQ=255)
                            caGGAAGACCGCAACACGTGACTGATTACGCGATAAGCAAGAAAGGAAAGCGATCGCTTTGGATGCCGAGGCTGGTATTTATCACACGCGCGGCCTGTGc                                                                    <  2:1718303/100‑1 (MQ=255)
                               gAAGACCGCAACACATGGCTGATTATCCAATAAGCAAGAAAAGCATGCGATCGCTTTGGGTCCCGCGTCTCGTATTCATTACTCTTCCGTACTGTTCCAg                                                                 <  2:558548/100‑1 (MQ=255)
                               gAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAg                                                                 >  1:916659/1‑100 (MQ=255)
                               gAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAg                                                                 <  1:2497587/100‑1 (MQ=255)
                               gAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAg                                                                 <  1:2270033/100‑1 (MQ=255)
                                caGACCGCAACACATGACTGATTCCGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGCTCCCGCGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgc                                                                <  2:1179997/99‑1 (MQ=255)
                                 aGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                               >  2:404359/1‑100 (MQ=255)
                                 aGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcg                                                               <  1:1766529/100‑1 (MQ=255)
                                  gACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAgcgc                                                              >  1:2423407/1‑100 (MQ=255)
                                   aCCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCa                                                             <  1:2792046/100‑1 (MQ=255)
                                    ccGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAg                                                            >  2:2338504/1‑100 (MQ=255)
                                    ccGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAg                                                            >  2:3521363/1‑100 (MQ=255)
                                     cGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAgg                                                           <  1:3967401/100‑1 (MQ=255)
                                      gCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTCTTCATTACCCTCGCGGCCTGTGCCAGCGCAGGt                                                          >  1:4034444/1‑100 (MQ=255)
                                      gCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGt                                                          >  2:796502/1‑100 (MQ=255)
                                         acacATGACTGATTACGTTATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTTGTATCCATTACCCTCGCGGCCTTTGCCAGCCCGGGTTAc                                                       <  2:3869815/100‑1 (MQ=255)
                                         acacATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTAc                                                       >  2:876743/1‑100 (MQ=255)
                                         acacATGACTGATTACGAGATAAGCAAGAAACGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTAc                                                       <  2:421752/100‑1 (MQ=255)
                                           acaTGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAg                                                     <  2:1607292/100‑1 (MQ=255)
                                            caTGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGc                                                    >  1:962005/1‑100 (MQ=255)
                                            caTGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGc                                                    <  2:1447104/100‑1 (MQ=255)
                                             aTGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCt                                                   >  1:2035476/1‑100 (MQ=255)
                                              tGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTa                                                  >  1:780841/1‑100 (MQ=255)
                                              tGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTa                                                  >  1:2376717/1‑100 (MQ=255)
                                               gACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTAc                                                 <  2:3880281/100‑1 (MQ=255)
                                                 cTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTg                                               <  1:3695801/100‑1 (MQ=255)
                                                 cTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTg                                               >  1:2655278/1‑100 (MQ=255)
                                                   gATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGc                                             <  2:1407109/100‑1 (MQ=255)
                                                    aTTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCa                                            >  1:3923701/1‑100 (MQ=255)
                                                     ctACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAt                                           <  1:580943/99‑1 (MQ=255)
                                                      tACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAtt                                          <  1:2596223/100‑1 (MQ=255)
                                                       aCGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCAtag                                         >  2:569174/1‑98 (MQ=255)
                                                       aCGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTc                                         >  2:1913433/1‑100 (MQ=255)
                                                          cgATAAGCAAGAAAAGCAAGCGATCACTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCa                                      <  1:2450671/100‑1 (MQ=255)
                                                            aTAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATc                                    <  2:506341/100‑1 (MQ=255)
                                                              aaGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAg                                  >  1:2266449/1‑100 (MQ=255)
                                                               aGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAgg                                 <  1:3433259/100‑1 (MQ=255)
                                                                gCAATAAAAGCGAGCGATCGCTTTGGATCTCGAGTCTGGTATGCATTAACCTCGCGGCTTGTGGCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGt                                >  2:646427/1‑100 (MQ=255)
                                                                gCAAGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGt                                >  1:900623/1‑100 (MQ=255)
                                                                  aaGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTg                              >  1:4024645/1‑100 (MQ=255)
                                                                   aGAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGc                             >  1:1038302/1‑100 (MQ=255)
                                                                    gAAAAGCAAGCGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGcc                            >  1:2741308/1‑100 (MQ=255)
                                                                        agcaatcGATCGCTTTGTATCCTGAGTCTAATATTCATTACCCTCGCGGGCTGTGCCAGTGCAGGTTACAGCAACTGGCATTCGCAGCAGCTTGCCGCtt                        <  2:247777/100‑2 (MQ=255)
                                                                         gcaagcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTga                       >  1:923799/1‑100 (MQ=255)
                                                                          caagcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgac                      <  1:31873/100‑1 (MQ=255)
                                                                          caagcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgac                      <  2:3914316/100‑1 (MQ=255)
                                                                          caagcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgac                      <  2:1326284/100‑1 (MQ=255)
                                                                           aagcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTgacg                     <  1:569251/100‑1 (MQ=255)
                                                                             gcGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGTATTCGCATCAGGTTGCCGCTgacgac                   >  2:3225655/1‑100 (MQ=255)
                                                                              cGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACa                  >  2:927242/1‑100 (MQ=255)
                                                                              cGATCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACa                  >  2:2060691/1‑100 (MQ=255)
                                                                                aTCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACaaa                >  2:2509122/1‑100 (MQ=255)
                                                                                 tCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAg               >  2:291514/1‑100 (MQ=255)
                                                                                 tCGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAg               <  2:2143859/100‑1 (MQ=255)
                                                                                  cGCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAgc              <  1:2264089/100‑1 (MQ=255)
                                                                                   gCTTTGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAgcg             <  2:3088101/100‑1 (MQ=255)
                                                                                    cTTTGGATCCCGAGTCTGGTATTCATTACCCTCGAGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAgcgc            <  2:1857756/100‑1 (MQ=255)
                                                                                      ttGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCgcag          <  1:595591/100‑1 (MQ=255)
                                                                                       tGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCgcagc         >  2:2663509/1‑100 (MQ=255)
                                                                                       tGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCgcagc         >  2:2207415/1‑100 (MQ=255)
                                                                                       tGGATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCgcagc         <  1:1914370/100‑1 (MQ=255)
                                                                                       tGGATCCCGAGCCTGGTATTCATTACCCTCGCGGACTGTGCCAGCGCAGGTTAAAGCTACTGGCATTCGCATCAGGTTTCCGCTGACGATAAAGCgcagc         >  2:3693171/1‑100 (MQ=255)
                                                                                        ggATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCgcagca        >  1:1048074/1‑100 (MQ=255)
                                                                                         gATCCCGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCaa       >  2:407628/1‑100 (MQ=255)
                                                                                              cGAGTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTATAGCTACTGACATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGcgt  >  1:3625107/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GATAATTAGCCGATAAGCAGTAGCGACACAGGAAGACCGCAACACATGACTGATTACGCGATAAGCAAGAAAAGCAAGCGATCGCTTTGGATCCCGATTCTGGTATTCATTACCCTCGCGGCCTGTGCCAGCGCAGGTTACAGCTACTGGCATTCGCATCAGGTTGCCGCTGACGACAAAGCGCAGCAACGCGT  >  NC_000913/2019573‑2019766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A