Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,036,444 T→G Y204D (TAT→GAT)  hchA → glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,036,4440TG95.9% 467.2 / 5.7 148Y204D (TAT→GAT) hchAglyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (62/79);  total (64/84)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 148 total aligned reads displayed.

ACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGA  >  NC_000913/2036347‑2036540
                                                                                                 |                                                                                                
aCAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGAt                                                                                                <  1:3971971/100‑1 (MQ=255)
 cAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGAtt                                                                                               >  1:430327/1‑100 (MQ=255)
   gTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTcc                                                                                             <  1:2867057/100‑1 (MQ=255)
     gggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAt                                                                                           >  1:3584177/1‑100 (MQ=255)
     gggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAt                                                                                           >  1:2827667/1‑100 (MQ=255)
     gggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAt                                                                                           >  2:1461077/1‑100 (MQ=255)
      ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAtt                                                                                          <  1:3298525/100‑1 (MQ=255)
      ggCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAtt                                                                                          >  2:1031031/1‑100 (MQ=255)
       gCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCAttt                                                                                         <  2:234604/100‑1 (MQ=255)
       gCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCAttt                                                                                         >  1:1647883/1‑100 (MQ=255)
        cAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTg                                                                                        <  2:3572905/100‑1 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGGTTTTCTGGCGCGTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                      >  2:3839004/1‑100 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                      <  2:966676/100‑1 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                      <  1:1728232/100‑1 (MQ=255)
          aTCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTgcg                                                                                      <  1:1298947/100‑1 (MQ=255)
            cAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCa                                                                                    >  2:2991634/1‑100 (MQ=255)
            cAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTGCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATGTGCGCa                                                                                    >  2:670582/1‑100 (MQ=255)
                aaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTcc                                                                                <  2:3368498/100‑1 (MQ=255)
                aaTGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTcc                                                                                <  2:1447538/100‑1 (MQ=255)
                  tGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCa                                                                              <  2:3104290/100‑1 (MQ=255)
                  tGACCGTTTTGTTATCTCCCTTCGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATACCCCACTGAATGGTGATTCCATTTGCGCATTCCCa                                                                              <  2:1579028/100‑1 (MQ=255)
                   gACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAg                                                                             <  1:1761391/100‑1 (MQ=255)
                    aCCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGa                                                                            >  1:878954/1‑100 (MQ=255)
                    aCCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGa                                                                            <  2:840771/100‑1 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                           <  1:1168995/100‑1 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                           <  1:522305/100‑1 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                           >  1:2614703/1‑100 (MQ=255)
                     ccGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAc                                                                           >  2:3816163/1‑100 (MQ=255)
                       gTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTCGCGCTTCGCCACGGCGATAACCCACTGAATGTTGATTCCATTTGCGCATTCCCAGAcgc                                                                         >  1:3534630/1‑100 (MQ=255)
                         tttGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGAcgccg                                                                       <  2:1089032/100‑1 (MQ=255)
                         tttGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTTCGCATTCCCAGAcgccg                                                                       >  1:3222619/1‑100 (MQ=255)
                         tttGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGAcgccg                                                                       <  2:370706/100‑1 (MQ=255)
                           tGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCa                                                                     >  1:3524067/1‑100 (MQ=255)
                           tGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGGGAATCCATTTGCGAATTCGCAGAcgccgag                                                                     >  1:3348084/1‑98 (MQ=255)
                           tGTTATCTCCCTTCGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCa                                                                     <  2:1076300/100‑1 (MQ=255)
                            gTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAg                                                                    <  2:3699314/100‑1 (MQ=255)
                            gTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAg                                                                    <  2:1578502/100‑1 (MQ=255)
                            gTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAg                                                                    <  2:1243684/100‑1 (MQ=255)
                            gTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAg                                                                    <  1:884870/100‑1 (MQ=255)
                              tATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGac                                                                  >  2:3623638/1‑100 (MQ=255)
                              tATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGac                                                                  <  1:3685796/100‑1 (MQ=255)
                               aTCCCCCTCTGCCACGGCCCGGCGGCTTTGCTGGTGCGTCGCTACGACGATAACCCACTGGATGGTGCTTCCATTTGCGCGTTCCCAGACGCCGGAGaca                                                                 <  2:2997069/100‑1 (MQ=255)
                                tctcCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaa                                                                <  1:687553/100‑1 (MQ=255)
                                   cccTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGacaaaca                                                             >  1:1887037/1‑100 (MQ=255)
                                    ccTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGacaaacaa                                                            >  2:623004/1‑100 (MQ=255)
                                     cTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGAcaaacaaa                                                           >  2:1348054/1‑100 (MQ=255)
                                      tttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACaaacaaac                                                          <  2:2687663/100‑1 (MQ=255)
                                      tttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACaaacaaac                                                          <  2:3111063/100‑1 (MQ=255)
                                      tttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACaaacaaac                                                          <  1:3202460/100‑1 (MQ=255)
                                       ttGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACg                                                         <  1:444118/100‑1 (MQ=255)
                                          ccACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCa                                                      >  1:2305758/1‑100 (MQ=255)
                                           cACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCag                                                     >  2:2187608/1‑100 (MQ=255)
                                               gCCCGGCGGCTTTTCTGGCGCTTCGCTACGGCGATAAACCACTGAATGTGGATTCCATTTCGGCATTGCCAGACGCCGCAGACAAAAAAACGCCAGACAt                                                 >  2:3166573/1‑100 (MQ=255)
                                                 ccGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTg                                               >  1:1960677/1‑100 (MQ=255)
                                                  cggcggCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTgg                                              <  2:324108/100‑1 (MQ=255)
                                                  cggcggCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTgg                                              <  1:204931/100‑1 (MQ=255)
                                                      ggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtat                                          <  2:3652780/100‑1 (MQ=255)
                                                      ggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtat                                          >  2:1723533/1‑100 (MQ=255)
                                                      ggcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtat                                          >  2:2825661/1‑100 (MQ=255)
                                                       gcTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCtata                                         <  2:1670906/100‑1 (MQ=255)
                                                        cTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTatat                                        >  1:1144494/1‑100 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                  <  2:910413/100‑1 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                  <  2:2019278/100‑1 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                  >  1:3155336/1‑100 (MQ=255)
                                                              tGGCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCggg                                  >  1:2362610/1‑100 (MQ=255)
                                                              tGGCGCTCCGCCACGGCGATAACCCACTGAACGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACGAACAAACGCCAGAGATTGGCTATATGCCggg                                  <  1:3265223/100‑1 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGCTTCCATTTGCGCATTCCCAGACGCAGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 >  2:2489731/1‑100 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 <  1:376921/100‑1 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 <  2:3300848/100‑1 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 <  2:1923828/100‑1 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATACCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 >  2:3937863/1‑100 (MQ=255)
                                                               ggCGCTTCGCCACGGCGATAAACCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGt                                 >  1:3480555/1‑100 (MQ=255)
                                                                gcgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTc                                <  2:786847/100‑1 (MQ=255)
                                                                gcgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTc                                >  2:933640/1‑100 (MQ=255)
                                                                gcgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTc                                >  2:3480583/1‑100 (MQ=255)
                                                                 cgcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCa                               <  1:1439232/100‑1 (MQ=255)
                                                                  gcTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAt                              >  2:3266294/1‑100 (MQ=255)
                                                                   cTTCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtc                             <  1:4026390/100‑1 (MQ=255)
                                                                    ttCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtct                            <  1:779782/100‑1 (MQ=255)
                                                                     tCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtctc                           <  1:3392725/100‑1 (MQ=255)
                                                                     tCGCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAtctc                           <  1:3223155/100‑1 (MQ=255)
                                                                       gCCACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAc                         >  2:2109719/1‑100 (MQ=255)
                                                                        ccACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCAcc                        <  2:2949218/100‑1 (MQ=255)
                                                                         cACGGCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCt                       >  2:1004604/1‑100 (MQ=255)
                                                                         cACGGCGATAACCCACTGAATGGTGATGCCAGTTGCGCATTCCCAGACGCCGGAGATAAACAAACGCCAGAGATTGGCTATATGCCGGGTCAGCTCACCt                       <  2:251524/100‑1 (MQ=255)
                                                                            ggCGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAGCGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGt                    <  2:2668772/100‑1 (MQ=255)
                                                                              cGATAACCCACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTAc                  <  2:1534742/100‑1 (MQ=255)
                                                                               gATAACCCACTGAATGGCGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTAACCTGGTACt                 <  2:915288/100‑1 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            <  1:3919297/100‑1 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            >  1:2731741/1‑100 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            >  2:3673205/1‑100 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            <  1:69511/100‑1 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            <  1:769170/100‑1 (MQ=255)
                                                                                    cccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGc            >  1:2246916/1‑100 (MQ=255)
                                                                                     ccACTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCg           >  1:55019/1‑100 (MQ=255)
                                                                                        cTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCgaag        <  2:475362/100‑1 (MQ=255)
                                                                                        cTGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCgaag        <  2:1873525/100‑1 (MQ=255)
                                                                                         tGAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCgaaga       <  2:3220537/100‑1 (MQ=255)
                                                                                          gAATGGTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCgaagaa      >  2:483230/1‑100 (MQ=255)
                                                                                              ggTGATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTga  >  2:3968539/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ACAGTGGGCAATCAAAAATGACCGTTTTGTTATCTCCCTTTGCCACGGCCCGGCGGCTTTTCTGGCGCTTCGCCACGGCGATAACCCACTGAATGGTTATTCCATTTGCGCATTCCCAGACGCCGCAGACAAACAAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGA  >  NC_000913/2036347‑2036540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A