Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,497,996 T→G E100D (GAA→GAC)  alaC ← glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,497,9960TG98.7% 543.1 / ‑2.4 156E100D (GAA→GAC) alaCglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (86/68);  total (87/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 156 total aligned reads displayed.

GGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAGTAACC  >  NC_000913/2497899‑2498091
                                                                                                 |                                                                                               
ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGGCGATGGCGTc                                                                                               >  2:2789648/1‑100 (MQ=255)
ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTc                                                                                               >  2:1032556/1‑100 (MQ=255)
  tAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTg                                                                                             >  2:2578506/1‑100 (MQ=255)
         gATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCAGATTCCgg                                                                                      >  1:3893260/1‑100 (MQ=255)
          aTTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCggg                                                                                     >  1:2238723/1‑100 (MQ=255)
           ttCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGt                                                                                    >  1:1402512/1‑100 (MQ=255)
             cGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGACCCAATAGTGACGATGGCGTCTGATTCCGGGTCg                                                                                  <  2:816526/100‑1 (MQ=255)
             cGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCg                                                                                  >  2:3224253/1‑100 (MQ=255)
               gCACCAGCACCGTGTCCCCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAt                                                                                <  1:3355810/100‑1 (MQ=255)
               gCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAt                                                                                <  2:3473451/100‑1 (MQ=255)
               gCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAt                                                                                <  2:3449787/100‑1 (MQ=255)
               gCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAAGCCCTCTTTCAAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAt                                                                                >  2:3628192/1‑100 (MQ=255)
                cACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAtt                                                                               >  2:2240824/1‑100 (MQ=255)
                cACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAtt                                                                               >  2:2876115/1‑100 (MQ=255)
                cACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAtt                                                                               <  2:3031359/100‑1 (MQ=255)
                 aCCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGAttt                                                                              >  1:933905/1‑100 (MQ=255)
                  ccAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTc                                                                             >  1:2383805/1‑100 (MQ=255)
                   cAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCa                                                                            <  2:830049/100‑1 (MQ=255)
                    aGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCaa                                                                           >  1:780961/1‑100 (MQ=255)
                     gCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAAc                                                                          <  2:3411242/100‑1 (MQ=255)
                          gtgtCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAAcgtcgt                                                                     >  1:1104/1‑100 (MQ=255)
                          gtgtCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAAcgtcgt                                                                     >  2:1422338/1‑100 (MQ=255)
                           tgtCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTa                                                                    <  1:2784360/100‑1 (MQ=255)
                            gtCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAg                                                                   <  1:1087549/100‑1 (MQ=255)
                            gtCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAg                                                                   <  1:3092229/100‑1 (MQ=255)
                             tCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGc                                                                  <  1:2585885/100‑1 (MQ=255)
                             tCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTGTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGc                                                                  <  1:900697/100‑1 (MQ=255)
                              cACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTTACGATGGCGTCTGATTCCGGGTGGATTTCAACGTCGTAGCg                                                                 >  2:2672843/1‑100 (MQ=255)
                              cACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCg                                                                 >  1:255348/1‑100 (MQ=255)
                              cACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCg                                                                 >  2:1906702/1‑100 (MQ=255)
                                ccATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGAt                                                               >  2:2901321/1‑100 (MQ=255)
                                ccATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGAt                                                               >  1:127546/1‑100 (MQ=255)
                                ccATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGAt                                                               >  2:2551204/1‑100 (MQ=255)
                                ccATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGAt                                                               >  1:1507129/1‑100 (MQ=255)
                                 cATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATc                                                              <  1:145658/100‑1 (MQ=255)
                                  aTGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATcc                                                             >  2:1196953/1‑100 (MQ=255)
                                   tGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCt                                                            <  1:2275653/100‑1 (MQ=255)
                                     aTCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGa                                                          >  1:1225407/1‑100 (MQ=255)
                                      tCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGAt                                                         <  1:696679/100‑1 (MQ=255)
                                      tCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGCCTGATTCCGGGTCGCTTTCAACCTCGTAACGCTCCTGAt                                                         >  2:342417/1‑100 (MQ=255)
                                       ccAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATa                                                        <  1:2609065/100‑1 (MQ=255)
                                       ccAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATa                                                        <  1:376969/100‑1 (MQ=255)
                                         aGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAcc                                                      >  1:1946603/1‑100 (MQ=255)
                                         aGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAcc                                                      >  2:2060912/1‑100 (MQ=255)
                                          gCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCa                                                     >  2:2340092/1‑100 (MQ=255)
                                          gCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCa                                                     >  2:3711306/1‑100 (MQ=255)
                                            gTCGCCAGCATCCGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGc                                                   >  1:3507512/1‑100 (MQ=255)
                                             tCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAAGCAATAGTGACGATAGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCg                                                  <  2:2362627/100‑1 (MQ=255)
                                              cGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCgg                                                 <  1:2478170/100‑1 (MQ=255)
                                              cGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCgg                                                 <  1:302106/100‑1 (MQ=255)
                                               gCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCggg                                                >  1:382443/1‑100 (MQ=255)
                                                ccAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGa                                               >  2:897677/1‑100 (MQ=255)
                                                ccAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGa                                               >  1:1557761/1‑100 (MQ=255)
                                                ccAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGa                                               >  1:160045/1‑100 (MQ=255)
                                                ccAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGa                                               <  1:2290375/100‑1 (MQ=255)
                                                   gCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAAt                                            <  1:986507/100‑1 (MQ=255)
                                                    cATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATg                                           >  2:3680529/1‑100 (MQ=255)
                                                    cATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATg                                           >  2:3227580/1‑100 (MQ=255)
                                                      tCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGgc                                         >  2:1362181/1‑100 (MQ=255)
                                                       cAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGgcg                                        >  1:3108340/1‑100 (MQ=255)
                                                       cAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGgcg                                        <  1:10068/100‑1 (MQ=255)
                                                          aTGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGa                                     >  1:826602/1‑100 (MQ=255)
                                                             cgcCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGt                                  >  1:3343982/1‑100 (MQ=255)
                                                             cgcCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGGCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGt                                  >  1:3878688/1‑100 (MQ=255)
                                                              gcCAGGCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTa                                 >  1:1978092/1‑100 (MQ=255)
                                                                   gCCCTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACcgc                            >  1:3227264/1‑100 (MQ=255)
                                                                    cccTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACcgcg                           <  1:3031968/100‑1 (MQ=255)
                                                                     ccTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCgg                          >  2:1766941/1‑100 (MQ=255)
                                                                     ccTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCgg                          <  1:47670/100‑1 (MQ=255)
                                                                     ccTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCgg                          <  1:2919899/100‑1 (MQ=255)
                                                                     ccTCTTTCGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGAGATCCTGATACCAGCGGGAAATGGCGAGACGTAACCGCgg                          >  2:1838219/1‑100 (MQ=255)
                                                                            cGAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCcg                   <  2:1915731/100‑1 (MQ=255)
                                                                             gAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCcgc                  >  1:3689682/1‑100 (MQ=255)
                                                                             gAACCAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCcgc                  >  2:502927/1‑100 (MQ=255)
                                                                                ccAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTg               >  1:1731791/1‑100 (MQ=255)
                                                                                ccAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTg               >  1:1403785/1‑100 (MQ=255)
                                                                                 cAATAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGa              >  1:1049227/1‑100 (MQ=255)
                                                                                  aaTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGCATTCcgcggtta             <  2:673594/100‑5 (MQ=255)
                                                                                   aTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAg            >  2:2531643/1‑100 (MQ=255)
                                                                                   aTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAg            >  1:700233/1‑100 (MQ=255)
                                                                                   aTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAg            >  2:2576529/1‑100 (MQ=255)
                                                                                   aTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAg            <  2:1242056/100‑1 (MQ=255)
                                                                                   aTAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAg            <  2:1061868/100‑1 (MQ=255)
                                                                                    tAGTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGt           <  2:207217/100‑1 (MQ=255)
                                                                                      gTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         <  1:3619931/100‑1 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAGGTgg         >  1:185528/1‑100 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         <  1:1503149/100‑1 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         >  1:3788841/1‑100 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         >  1:2563551/1‑100 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         >  1:3438435/1‑100 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         <  1:3445447/100‑1 (MQ=255)
                                                                                      gTGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTgg         <  2:1986190/100‑1 (MQ=255)
                                                                                       tGACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGa        <  1:2030266/100‑1 (MQ=255)
                                                                                        gaggATGGCGGCTGATTGCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGCGGAg       <  1:3663690/97‑1 (MQ=255)
                                                                                        gACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAg       >  1:1768021/1‑100 (MQ=255)
                                                                                        gACGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAg       >  2:2187465/1‑100 (MQ=255)
                                                                                         aCGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAGt      <  1:2093394/100‑1 (MQ=255)
                                                                                         aCGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAGt      <  2:507616/100‑1 (MQ=255)
                                                                                         aCGATGGCGTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGAGACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAGt      <  2:637970/100‑1 (MQ=255)
                                                                                             tGGCGTTTGATTCCGGGTCGATTTCAACCTCCTAGCGATCCTGATACCAGCGGGAAATCGCGCGACGTAACCGCCGAATGCCGCGTGAAGTGGAGTAAcc  <  2:2404721/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAAGTGGAGTAACC  >  NC_000913/2497899‑2498091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A