Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,452 A→C Q398P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,4520AC98.6% 508.6 / ‑4.1 143Q398P (CAG→CCG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (59/81);  total (60/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGT  >  NC_000913/3972356‑3972548
                                                                                                |                                                                                                
ngTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGtt                                                                                               >  1:3236907/2‑100 (MQ=255)
aGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGtt                                                                                               <  2:258285/100‑1 (MQ=255)
 gTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTc                                                                                              <  1:1624288/100‑1 (MQ=255)
   tGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCaa                                                                                            >  2:581194/1‑100 (MQ=255)
    gCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCaaa                                                                                           <  1:1441915/100‑1 (MQ=255)
     ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAg                                                                                          <  1:474712/100‑1 (MQ=255)
      cGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGt                                                                                         >  1:1068782/1‑100 (MQ=255)
      cGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGt                                                                                         >  1:3330475/1‑100 (MQ=255)
      cGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGt                                                                                         >  1:3773234/1‑100 (MQ=255)
      cGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGt                                                                                         >  2:686603/1‑100 (MQ=255)
       gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  1:2892661/1‑100 (MQ=255)
       gAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGtt                                                                                        >  1:2495704/1‑100 (MQ=255)
        aaaaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTa                                                                                       <  1:3406059/100‑1 (MQ=255)
         aaaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTAt                                                                                      >  2:3095952/1‑100 (MQ=255)
          aagaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTTGATCATAGTCCGTTCAAAGTTATc                                                                                     <  1:77582/97‑1 (MQ=255)
          aaaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATc                                                                                     <  2:3824858/100‑1 (MQ=255)
           aaaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCa                                                                                    >  1:651648/1‑100 (MQ=255)
            aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCaa                                                                                   >  2:212234/1‑100 (MQ=255)
            aaCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCaa                                                                                   <  1:3394974/100‑1 (MQ=255)
             aCTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAAt                                                                                  <  2:1948882/100‑1 (MQ=255)
              cTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATg                                                                                 >  2:343619/1‑100 (MQ=255)
              cTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCGGGCAACGGCAGACGTGCTGGTGATGCGGGTCGATCATAGTCCGTTCAAAGTTATCAATg                                                                                 >  1:2660428/1‑100 (MQ=255)
                gACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGc                                                                               <  2:661602/100‑1 (MQ=255)
                gACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGc                                                                               <  1:2182268/100‑1 (MQ=255)
                 aCCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCATACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCg                                                                              <  1:1892134/100‑1 (MQ=255)
                 aCCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCg                                                                              <  1:2315347/100‑1 (MQ=255)
                       cTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATg                                                                        >  1:3288909/1‑100 (MQ=255)
                         ttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTc                                                                      >  2:1936570/1‑100 (MQ=255)
                         ttGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTc                                                                      >  1:3079146/1‑100 (MQ=255)
                           gTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCa                                                                    <  1:2718199/100‑1 (MQ=255)
                             aCTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATc                                                                  >  2:1414861/1‑100 (MQ=255)
                              ctctGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATca                                                                 >  2:828495/1‑100 (MQ=255)
                              ctctGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATca                                                                 >  1:1183597/1‑100 (MQ=255)
                               tctGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATcag                                                                <  2:3475370/100‑1 (MQ=255)
                                   gcgcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGt                                                            <  1:2948674/100‑1 (MQ=255)
                                    cgcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTa                                                           >  2:215734/1‑100 (MQ=255)
                                    cgcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAATa                                                           >  2:2898268/1‑100 (MQ=255)
                                     gcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAt                                                          <  2:2705530/100‑1 (MQ=255)
                                     gcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAt                                                          <  1:2006628/100‑1 (MQ=255)
                                     gcAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAt                                                          <  1:2120388/100‑1 (MQ=255)
                                        gCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtc                                                       >  1:3631794/1‑100 (MQ=255)
                                        gCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtc                                                       >  1:870755/1‑100 (MQ=255)
                                         cTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcg                                                      <  1:311150/100‑1 (MQ=255)
                                         cTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcg                                                      <  2:2582120/100‑1 (MQ=255)
                                         cTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcg                                                      <  2:1294163/100‑1 (MQ=255)
                                         cTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcg                                                      <  1:2466040/100‑1 (MQ=255)
                                         cTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTAGgtcg                                                      >  1:3660810/1‑100 (MQ=255)
                                           ngACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                                    >  1:3249618/2‑100 (MQ=255)
                                              cGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGAt                                                 <  1:2981769/100‑1 (MQ=255)
                                              cGAGGCGCCGGAAACGGCAGACGTGCTGGTGCTGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGAt                                                 <  2:2406472/100‑1 (MQ=255)
                                               gAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGAtn                                                <  2:477652/100‑2 (MQ=255)
                                                gggCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTAAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGc                                               <  2:1956232/99‑1 (MQ=255)
                                                aGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTTAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGc                                               <  1:1928085/100‑1 (MQ=255)
                                                aGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGc                                               >  1:1371484/1‑100 (MQ=255)
                                                    gcTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCaaa                                           >  1:2244255/1‑100 (MQ=255)
                                                     cTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAg                                          <  1:394995/100‑1 (MQ=255)
                                                      tGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAgg                                         <  2:3693352/100‑1 (MQ=255)
                                                      tGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAgg                                         <  2:214122/100‑1 (MQ=255)
                                                      tGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAgg                                         <  1:2112589/100‑1 (MQ=255)
                                                       ggCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGa                                        >  2:3912941/1‑100 (MQ=255)
                                                       ggCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGa                                        >  1:1543899/1‑100 (MQ=255)
                                                         cAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGt                                      >  2:3676993/1‑100 (MQ=255)
                                                          aaCGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTc                                     <  1:3806258/100‑1 (MQ=255)
                                                           aCGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCt                                    >  2:1103812/1‑100 (MQ=255)
                                                           aCGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCt                                    <  1:846312/100‑1 (MQ=255)
                                                              gCAGACGTGCTGGTGATGCTGGTCGATCACAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgc                                 <  1:3725513/100‑1 (MQ=255)
                                                              acAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgc                                 <  1:4080526/99‑1 (MQ=255)
                                                               cAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgcg                                <  1:2598833/100‑1 (MQ=255)
                                                               cAGACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgcg                                >  1:3759431/1‑100 (MQ=255)
                                                                 gACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCt                              <  1:2372752/100‑1 (MQ=255)
                                                                 gACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCt                              >  2:4001043/1‑100 (MQ=255)
                                                                 gACGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCt                              <  2:1187552/100‑1 (MQ=255)
                                                                  aCGTGCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtg                             >  2:1103380/1‑100 (MQ=255)
                                                                    gTGCTGGTGATGCTGATCGATGATACGGAGGTCAAAGTTGTCAATGGCGACGATGTCCATCAGCAGTATTTCGTCGATGCCAAAGGAGACGGGCGCtgat                           >  2:1995806/1‑100 (MQ=255)
                                                                     tGCTGGTGATGCTGGTCGATCATAGTCCGTACAAAGTTATCAATGGCGACAATGTTCATCAGCAGTATGTCGTCGATGCCAAAGGACTCTGGCGCtgatg                          <  2:2502418/100‑1 (MQ=255)
                                                                      gCTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtgatga                         <  2:763470/100‑1 (MQ=255)
                                                                       cTGGTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATgag                        >  2:157405/1‑100 (MQ=255)
                                                                         ggTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGaa                      >  1:3432975/1‑100 (MQ=255)
                                                                         ggTGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGCCTGGCGCTGATGAGaa                      >  1:3291229/1‑100 (MQ=255)
                                                                           tGATGCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGaaaa                    <  1:2527707/100‑1 (MQ=255)
                                                                               gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                <  1:119997/100‑1 (MQ=255)
                                                                               gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                <  2:618220/100‑1 (MQ=255)
                                                                               gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                >  2:3421847/1‑100 (MQ=255)
                                                                               gCTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTc                <  2:1517525/100‑1 (MQ=255)
                                                                                cTGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCt               <  1:4085136/100‑1 (MQ=255)
                                                                                 tGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTg              <  1:160049/100‑1 (MQ=255)
                                                                                 tGGTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTg              <  1:2749352/100‑1 (MQ=255)
                                                                                  ggTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGa             >  2:320174/1‑100 (MQ=255)
                                                                                  ggTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGa             >  1:3337791/1‑100 (MQ=255)
                                                                                   gTCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCAATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGAt            <  2:2066988/100‑1 (MQ=255)
                                                                                    tCGATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATa           >  1:1527961/1‑100 (MQ=255)
                                                                                      gATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAAc         <  2:3186786/100‑1 (MQ=255)
                                                                                      gATCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAAc         <  2:2425830/100‑1 (MQ=255)
                                                                                       aTCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACa        <  2:3815840/100‑1 (MQ=255)
                                                                                        tCATAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAg       <  1:1681784/100‑1 (MQ=255)
                                                                                          aTAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggt     <  1:170101/100‑1 (MQ=255)
                                                                                          aTAGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggt     <  2:1923480/100‑1 (MQ=255)
                                                                                            aGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggtgg   <  2:223031/100‑1 (MQ=255)
                                                                                            aGTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggtgg   <  1:1661330/100‑1 (MQ=255)
                                                                                             gTCCGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAggtggt  >  2:1158720/1‑100 (MQ=255)
                                                                                                |                                                                                                
AGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAATTCTGATAACAGGTGGT  >  NC_000913/3972356‑3972548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A