Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I53 R1	229	158.0	8090706	91.1%	7370633	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,190,100	T→G	I105M (ATT→ATG)	yjaZ →	stationary phase growth adaptation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,190,100	0	T	G	98.6%	486.7 / ‑2.9	139	I105M (ATT→ATG)	yjaZ	stationary phase growth adaptation protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (61/76); total (62/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 142 total aligned reads displayed.

GCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAG  >  NC_000913/4190002‑4190196

tcgcCTAACGAATCTCGACCCGTCAGCCAGCACCTGTATTTCTTAACGCGCGCACTCTTGAAAATAACTATTTTGGCGCTGCCAGAAAGCACCGTTATTc                                                                                                 <  2:1659700/99‑1 (MQ=255)

  gcCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCAt                                                                                               <  2:3027462/100‑1 (MQ=255)

   ccTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATg                                                                                              >  1:3031233/1‑100 (MQ=255)

   ccTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATg                                                                                              >  1:3021687/1‑100 (MQ=255)

    cTAAAGAATCTCGTCCCTTCTGCCAGCACCGGTATAACTTAGCGCGCACACACTCAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTatgcatgc                                                                                             >  2:3807954/1‑100 (MQ=255)

     tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGcc                                                                                            <  1:3504356/100‑1 (MQ=255)

      aaaGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGACCTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCg                                                                                           >  1:1397801/1‑100 (MQ=255)

       aaGCATCGCCTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATACCgg                                                                                          <  2:3381853/100‑1 (MQ=255)

         gAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGt                                                                                        >  2:2854789/1‑100 (MQ=255)

           aTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTAt                                                                                      >  1:526589/1‑100 (MQ=255)

           aTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTAt                                                                                      <  2:1309038/100‑1 (MQ=255)

             ctcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATgg                                                                                    <  1:1280870/100‑1 (MQ=255)

              tcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGACAGCAACGTTATGCATGCCGGGTATGGt                                                                                   <  1:1881995/100‑1 (MQ=255)

              tcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGt                                                                                   <  1:4082865/100‑1 (MQ=255)

              tcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGt                                                                                   >  1:306916/1‑100 (MQ=255)

              tcGTCCCTTCTGCCAGCACCGTTATAACCGAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGt                                                                                   <  2:1818125/100‑1 (MQ=255)

               cGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTa                                                                                  >  2:331696/1‑100 (MQ=255)

               cGTCCCTTCTGCAAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTa                                                                                  >  2:3847825/1‑100 (MQ=255)

                gTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAg                                                                                 >  1:2460104/1‑100 (MQ=255)

                gTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAg                                                                                 >  1:3593326/1‑100 (MQ=255)

                   ccTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTa                                                                              >  2:1509414/1‑100 (MQ=255)

                   ccTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTa                                                                              >  1:2224398/1‑100 (MQ=255)

                    cTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTAt                                                                             <  1:589529/100‑1 (MQ=255)

                    cTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTAt                                                                             <  1:2014573/100‑1 (MQ=255)

                    cTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTAt                                                                             <  2:1036643/100‑1 (MQ=255)

                    cTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTAt                                                                             <  1:3725308/100‑1 (MQ=255)

                    cTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTAt                                                                             <  1:1207255/100‑1 (MQ=255)

                     ttCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATg                                                                            <  2:1405400/100‑1 (MQ=255)

                       cTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGcc                                                                          <  1:1354219/100‑1 (MQ=255)

                       cTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGcc                                                                          <  2:2053225/100‑1 (MQ=255)

                        tGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCg                                                                         <  2:754531/100‑1 (MQ=255)

                          ccAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTg                                                                       <  1:3560423/100‑1 (MQ=255)

                          ccAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTg                                                                       >  2:2936361/1‑100 (MQ=255)

                          ccAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTg                                                                       >  1:1625784/1‑100 (MQ=255)

                           cAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGa                                                                      >  2:431685/1‑100 (MQ=255)

                            aGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTGTGCATGCCGGGTATGGTAGCTATGCCGTGAt                                                                     <  1:3274763/100‑1 (MQ=255)

                            aGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTTAt                                                                     <  2:1811849/100‑1 (MQ=255)

                            aGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGAt                                                                     <  2:1677556/100‑1 (MQ=255)

                            aGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGAt                                                                     >  1:1361919/1‑100 (MQ=255)

                              cACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTg                                                                   <  2:1046259/100‑1 (MQ=255)

                               aCCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGa                                                                  >  1:1931138/1‑100 (MQ=255)

                                ccGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTCGCTATGCCGTGATTGAg                                                                 >  2:216703/1‑100 (MQ=255)

                                ccGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAg                                                                 >  1:2831524/1‑100 (MQ=255)

                                  gTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGt                                                               >  1:1287505/1‑100 (MQ=255)

                                   ttATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAACGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                              >  2:1543844/1‑100 (MQ=255)

                                   ttATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                              >  1:3477884/1‑100 (MQ=255)

                                    tgtaACTTAGCGCGCACAGACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTgg                                                             >  2:491736/3‑100 (MQ=255)

                                    tataACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTgg                                                             <  1:2858461/100‑1 (MQ=255)

                                    tataACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTgg                                                             >  1:2643677/1‑100 (MQ=255)

                                      taACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTgaga                                                           >  2:584543/1‑97 (MQ=255)

                                      taACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGAc                                                           <  2:1208207/100‑1 (MQ=255)

                                         cTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAACGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTa                                                        >  1:2255635/1‑100 (MQ=255)

                                         cTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTa                                                        >  1:2000506/1‑100 (MQ=255)

                                            aGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGAc                                                     >  2:2439565/1‑100 (MQ=255)

                                                 gcACACACTTAAACAGCACTATTTTGGCGCTGCCAGAATGCAACGTTATGCATGCCGGGGATGGTAGCTATGCCGTGATTGTGGTGGACTTAGACggagg                                                <  2:3036754/100‑1 (MQ=255)

                                                 gcACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagg                                                <  1:294213/100‑1 (MQ=255)

                                                  cacacaCTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagga                                               >  1:2181196/1‑100 (MQ=255)

                                                  cacacaCTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagga                                               >  1:1089959/1‑100 (MQ=255)

                                                  cacacaCTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagga                                               <  1:3929713/100‑1 (MQ=255)

                                                   acacacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGgaggag                                              >  2:2941467/1‑100 (MQ=255)

                                                    cacacTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGgaga                                             >  1:897317/1‑100 (MQ=255)

                                                        cTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAg                                         >  2:3447995/1‑100 (MQ=255)

                                                        cTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAg                                         >  1:3387776/1‑100 (MQ=255)

                                                         ttAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAgg                                        >  1:314404/1‑100 (MQ=255)

                                                          tAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGc                                       <  2:1588553/100‑1 (MQ=255)

                                                           aaaaaGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCa                                      >  1:3689081/1‑100 (MQ=255)

                                                                gAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTa                                 >  2:3163489/1‑100 (MQ=255)

                                                                gAACTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTa                                 <  1:1901475/100‑1 (MQ=255)

                                                                  aCTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACt                               >  1:2403271/1‑100 (MQ=255)

                                                                  aCTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACt                               >  1:153839/1‑100 (MQ=255)

                                                                  aCTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACt                               <  1:1667708/100‑1 (MQ=255)

                                                                   cTATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCTGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACtt                              >  1:1446548/1‑100 (MQ=255)

                                                                    tATTTTGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACttt                             <  1:2261097/100‑1 (MQ=255)

                                                                      ttttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgt                           <  2:1278666/100‑1 (MQ=255)

                                                                        ttGGCGCTGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgttg                         <  1:1898169/100‑1 (MQ=255)

                                                                         tGGCGCTGCCAGAAAGCAACGGTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGc                        <  2:1745306/100‑1 (MQ=255)

                                                                            cgcTGCCAGAAAGCAACGTTATGAATGCCGGGGATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTATTTTGTTGCGtt                     >  2:2958515/1‑100 (MQ=255)

                                                                               tGCCAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAg                  <  2:1472100/100‑1 (MQ=255)

                                                                                  naGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc               >  1:3693305/2‑100 (MQ=255)

                                                                                  cAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgggn               <  2:1738177/100‑2 (MQ=255)

                                                                                  cAGAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGc               <  1:905622/100‑1 (MQ=255)

                                                                                    gAAAGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCtt             <  1:2239324/100‑1 (MQ=255)

                                                                                     aaaGCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCttt            <  1:3609171/100‑1 (MQ=255)

                                                                                       aGCAACGTTATGGACGCCGGGTATGGGAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCa          <  2:3649850/100‑1 (MQ=255)

                                                                                        gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg         >  2:2166936/1‑100 (MQ=255)

                                                                                        gCAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAg         <  1:1627302/100‑1 (MQ=255)

                                                                                         cAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGTTTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAgg        <  2:3629255/100‑1 (MQ=255)

                                                                                         cAACGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAgn        <  1:1891713/100‑2 (MQ=255)

                                                                                         cAACGTGATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAgg        >  2:3507547/1‑100 (MQ=255)

                                                                                          aaCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAggg       <  1:399874/100‑1 (MQ=255)

                                                                                          aaCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAggg       <  1:703749/100‑1 (MQ=255)

                                                                                          aaCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAggg       <  1:667203/100‑1 (MQ=255)

                                                                                          aaCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAggg       <  1:2609385/100‑1 (MQ=255)

                                                                                           aCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGga      >  1:1686561/1‑100 (MQ=255)

                                                                                           aCGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGga      <  1:1136326/100‑1 (MQ=255)

                                                                                            cGTTATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaa     >  2:3792313/1‑100 (MQ=255)

                                                                                              ttATGCATGCCGGTTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGTAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaa   <  1:4075324/100‑1 (MQ=255)

                                                                                              ttATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaa   >  1:1851429/1‑100 (MQ=255)

                                                                                               tATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaag  >  1:635948/1‑100 (MQ=255)

                                                                                               tATGCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGgaaaag  >  1:3535339/1‑100 (MQ=255)

GCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGGCTTTCAGGGAAAAG  >  NC_000913/4190002‑4190196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A