Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,313,090 T→G intergenic (‑99/‑260) alsR ← / → rpiB d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,313,0900TG97.8% 623.1 / 2.7 186intergenic (‑99/‑260)alsR/rpiBd‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (102/79);  total (105/81)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

Only 100 of 198 total aligned reads displayed.

CGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGA  >  NC_000913/4312994‑4313186
                                                                                                |                                                                                                
cGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtat                                                                                               >  2:2579488/1‑100 (MQ=255)
 gTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtatt                                                                                              >  2:947178/1‑100 (MQ=255)
 gTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtatt                                                                                              <  1:2195837/100‑1 (MQ=255)
 gTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtatt                                                                                              >  2:1577363/1‑100 (MQ=255)
  ttCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtattt                                                                                             <  2:1761982/100‑1 (MQ=255)
   attctTGACCTTGAATTGAAGACGCCACTATGTCAAAATATCAAGAACAGAAATGATGCTGGCGAATAAAAAAAAAAATAAAATTTATAAAGtgtatttt                                                                                            <  2:3263838/98‑1 (MQ=255)
    ctctTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGtgtatttta                                                                                           >  1:888596/1‑100 (MQ=255)
       ttGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATtg                                                                                        <  2:373181/100‑1 (MQ=255)
       ttGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATtg                                                                                        <  2:762823/100‑1 (MQ=255)
        tGTCCTTGAATTGAAGACGCCACTCGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATtgt                                                                                       <  2:2004782/100‑1 (MQ=255)
        tGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATtgt                                                                                       >  2:3284480/1‑100 (MQ=255)
         gTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATtgtg                                                                                      >  2:2100072/1‑100 (MQ=255)
           ccTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGaa                                                                                    <  1:790909/100‑1 (MQ=255)
            cTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAAc                                                                                   >  2:310528/1‑100 (MQ=255)
             ttgaattgaaGACGCGATGTTATCGCCAAAGCAAGAACAGAAAAGATGCTGGAGAATTTAAAAAAAAATAAAACTGATGATGTGTATTTTCTtgtagaga                                                                                  >  2:452032/1‑95 (MQ=255)
              tgaattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACaa                                                                                 >  1:2701234/1‑100 (MQ=255)
                aattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAAGAATg                                                                               >  1:3662084/1‑100 (MQ=255)
                aattgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATg                                                                               >  2:2393717/1‑100 (MQ=255)
                aatcgaaGACCCCACCAGGCCCCCACATCAAGAACACCAAATATGCGGGCGACTGCGCATAAAAAGAAAATGTTTTAAGTGTATTTTATTGTGAACAATg                                                                               <  2:1791543/100‑1 (MQ=255)
                   tgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCg                                                                            <  2:3142788/100‑1 (MQ=255)
                   tgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCg                                                                            >  1:563863/1‑100 (MQ=255)
                   tgaaGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCg                                                                            <  1:3875549/100‑1 (MQ=255)
                        aCGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTgg                                                                       >  1:2595075/1‑100 (MQ=255)
                         cGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGc                                                                      >  2:849453/1‑100 (MQ=255)
                         cGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGCGAACAATGGCGAGTGGc                                                                      >  2:3441760/1‑100 (MQ=255)
                          gCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGGACAATGGCGAGTGGCt                                                                     >  1:3358663/1‑100 (MQ=255)
                           ccACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCtt                                                                    >  1:1623054/1‑100 (MQ=255)
                           ccACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCtt                                                                    >  1:1889144/1‑100 (MQ=255)
                             aCTATATCACCACAGCAAGAGCAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGTCTTaa                                                                  >  2:3587200/1‑100 (MQ=255)
                                atatCACCACAGCAAGAACAGAAAAGATGCTGGTGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAt                                                               <  2:2070501/100‑1 (MQ=255)
                                atatCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAt                                                               <  1:1023588/100‑1 (MQ=255)
                                 tatCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtt                                                              <  2:109152/100‑1 (MQ=255)
                                   tCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtttt                                                            >  1:548627/1‑100 (MQ=255)
                                   tCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAAtttt                                                            <  1:3105261/100‑1 (MQ=255)
                                    caccacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTa                                                           <  2:220465/100‑1 (MQ=255)
                                    caccacAGCAAGAACAGAAAAGATGCCGGCCACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTa                                                           <  2:496126/100‑1 (MQ=255)
                                     accacAGCTAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGTCTTAAAATTTTAt                                                          >  2:1906069/1‑100 (MQ=255)
                                     accacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAt                                                          <  2:1828246/100‑1 (MQ=255)
                                     accacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAt                                                          <  1:4060628/100‑1 (MQ=255)
                                     accacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAt                                                          <  2:3206497/100‑1 (MQ=255)
                                     accacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAt                                                          >  1:1960402/1‑100 (MQ=255)
                                     accacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAt                                                          <  1:1918577/100‑1 (MQ=255)
                                      ccacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAtt                                                         >  2:2521901/1‑100 (MQ=255)
                                      ccacAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAtt                                                         >  2:1546274/1‑100 (MQ=255)
                                       cacaGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTAttt                                                        >  2:3634692/1‑100 (MQ=255)
                                       cacaGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCAAGTGGCTTAAAATTTTAttt                                                        <  2:128702/100‑1 (MQ=255)
                                         caGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCa                                                      >  2:1458203/1‑100 (MQ=255)
                                            cAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAACGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCAttt                                                   >  2:3841811/1‑100 (MQ=255)
                                            cAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAAACATGGCGAGTGGATTAAAATTTAATTTCCttc                                                   >  2:1170213/1‑99 (MQ=255)
                                             aaGAACAGAAAAGATGCTGGCAACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTa                                                  <  1:765798/100‑1 (MQ=255)
                                               gAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAt                                                >  2:889476/1‑100 (MQ=255)
                                                aaCAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAtt                                               >  1:3220140/1‑100 (MQ=255)
                                                  cAGAAAAGATGCTGGCGACTTCACAAAAAAAAAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAg                                             >  1:3267554/1‑100 (MQ=255)
                                                   aGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGt                                            <  2:262013/100‑1 (MQ=255)
                                                   aGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGt                                            >  2:2463817/1‑100 (MQ=255)
                                                   aGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGAGTATTTTATTGTGAACAATGGCGAGAGGCTTAAAATTTTATTTCATTTAATTAGt                                            >  1:1817292/1‑100 (MQ=255)
                                                    gAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGtt                                           >  2:1197997/1‑100 (MQ=255)
                                                    gAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGtt                                           >  2:3572002/1‑100 (MQ=255)
                                                    gAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGtt                                           >  1:2316399/1‑100 (MQ=255)
                                                    gAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGtt                                           >  1:2990645/1‑100 (MQ=255)
                                                           tGCTGGCGACTTCACAAAAACATAAAATTTATAAAGTGTATTCTATTATCAACAATAGCGCGTTGCTTACAATTTTATTTCATTTAATTAGTTTAAAAAc                                    >  2:1701394/1‑100 (MQ=255)
                                                             cTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACat                                  >  1:1139659/1‑100 (MQ=255)
                                                             cTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACat                                  >  2:570647/1‑100 (MQ=255)
                                                             cTGGCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACat                                  >  1:3606929/1‑100 (MQ=255)
                                                              tGGCGACTTCACAAAAAAATAAAATTTAGAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACatg                                 <  1:1064397/100‑1 (MQ=255)
                                                               ggCGACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACatga                                >  2:2647833/1‑100 (MQ=255)
                                                                  gACTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGt                             <  1:1303067/100‑1 (MQ=255)
                                                                   acataacaAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGtt                            <  2:642871/95‑1 (MQ=255)
                                                                   aCTTCACAAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGtt                            >  1:337570/1‑100 (MQ=255)
                                                                       cacaAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAttt                        <  2:3011005/100‑1 (MQ=255)
                                                                        acaAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTg                       <  1:930622/100‑1 (MQ=255)
                                                                         caAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGt                      >  1:400619/1‑100 (MQ=255)
                                                                         caAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGt                      >  2:3174783/1‑100 (MQ=255)
                                                                         caAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGt                      >  1:1340454/1‑100 (MQ=255)
                                                                         caAAAAAATAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTACTTGt                      >  1:1525905/1‑100 (MQ=255)
                                                                          aaaaaaaTAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtt                     <  2:2436265/100‑1 (MQ=255)
                                                                          aaaaaaaTAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtt                     <  2:393210/100‑1 (MQ=255)
                                                                          aaaaaaaTAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtt                     >  2:3363449/1‑100 (MQ=255)
                                                                           aaaaaaTAAAATTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGttt                    <  1:1209190/100‑1 (MQ=255)
                                                                                ataaaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTg               <  1:2029302/100‑1 (MQ=255)
                                                                                ataaaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTg               <  2:1897873/100‑1 (MQ=255)
                                                                                ataaaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTg               <  2:3401558/100‑1 (MQ=255)
                                                                                ataaaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTg               <  1:2414880/100‑1 (MQ=255)
                                                                                ataaaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTg               <  1:1436999/100‑1 (MQ=255)
                                                                                   aaatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtt            >  1:2269737/1‑100 (MQ=255)
                                                                                    aatTTATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGttt           <  2:3305469/100‑1 (MQ=255)
                                                                                      tttATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGttttt         <  2:1050001/100‑1 (MQ=255)
                                                                                       ttATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt        >  2:211755/1‑100 (MQ=255)
                                                                                       ttATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt        <  1:1431866/100‑1 (MQ=255)
                                                                                       ttATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt        <  1:2133006/100‑1 (MQ=255)
                                                                                       ttATAAAGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGtttttt        <  2:1410683/100‑1 (MQ=255)
                                                                                           aaaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgat    <  1:659012/100‑1 (MQ=255)
                                                                                           aaaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgat    <  2:3560744/100‑1 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   >  2:3216368/1‑100 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   >  2:5491/1‑100 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   <  1:3428845/100‑1 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   >  2:2903452/1‑100 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   <  1:3321342/100‑1 (MQ=255)
                                                                                            aaGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatg   <  1:977326/100‑1 (MQ=255)
                                                                                             aGTGTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgatga  <  2:1758528/100‑1 (MQ=255)
                                                                                                |                                                                                                
CGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGA  >  NC_000913/4312994‑4313186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A