Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I3 R1 53 201.3 10250884 92.1% 9441064 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 243,264 C→T G14S (GGC→AGC)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913243,2640CT99.1% 742.5 / ‑3.9 217G14S (GGC→AGC) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (98/117);  total (99/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 217 total aligned reads displayed.

ACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCCGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTAG  >  NC_000913/243168‑243359
                                                                                                |                                                                                               
aCGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAg                                                                                              <  2:1350740/100‑1 (MQ=255)
   ccGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAg                                                                                           <  1:2599894/100‑1 (MQ=255)
   ccGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAg                                                                                           >  1:843576/1‑100 (MQ=255)
    cGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAgg                                                                                          <  1:1943259/100‑1 (MQ=255)
    cGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAgg                                                                                          >  1:1738657/1‑100 (MQ=255)
       ncacaGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACa                                                                                       >  1:4122273/2‑100 (MQ=255)
            gACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGt                                                                                  >  2:70418/1‑100 (MQ=255)
            gACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGt                                                                                  >  2:1548968/1‑100 (MQ=255)
             aCCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTa                                                                                 >  1:2987029/1‑100 (MQ=255)
              cgaGCAACGCCCAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCGGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACGACCGTAg                                                                                >  2:4714045/3‑100 (MQ=255)
              ccAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAg                                                                                >  2:3724653/1‑100 (MQ=255)
              ccAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAg                                                                                >  1:2099878/1‑100 (MQ=255)
              ccAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAg                                                                                >  2:2347333/1‑100 (MQ=255)
              ccAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAg                                                                                >  1:3772424/1‑100 (MQ=255)
                aGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCg                                                                              >  2:2151185/1‑100 (MQ=255)
                 gCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCga                                                                             >  1:2929599/1‑100 (MQ=255)
                   aaCGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATGAGCTCACGCGGTGCTAGAACAACGCGCTGAGCAGGGCAACCGTAGCgaga                                                                           <  2:1839353/100‑1 (MQ=255)
                     cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                         <  2:2870981/100‑1 (MQ=255)
                     cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                         <  1:3898847/100‑1 (MQ=255)
                     cGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAAt                                                                         <  2:4654924/100‑1 (MQ=255)
                       ccGAGGGCGGCTGTCGAGGCGAGCAAAATCAGACTGCTGATAAATTAGCGCACGCGGGGAGATAGCAACGCTCTGAGCAGGACAACCGGAGCGAGTATAc                                                                       <  2:1757933/100‑1 (MQ=255)
                       ccGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATAc                                                                       <  2:3000577/100‑1 (MQ=255)
                        cGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACt                                                                      <  1:895482/100‑1 (MQ=255)
                         gAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTc                                                                     >  1:4408974/1‑100 (MQ=255)
                          aGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCa                                                                    <  2:2646423/100‑1 (MQ=255)
                             tcggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCaaaa                                                                 <  2:900089/99‑1 (MQ=255)
                              cggcTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCaaaaa                                                                >  1:914605/1‑99 (MQ=255)
                                 cTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcat                                                             >  1:229114/1‑100 (MQ=255)
                                 cTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcat                                                             <  1:4479210/100‑1 (MQ=255)
                                  tGTCCAGGCGAGCAAAATCAGACTGCTGATAAATACGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcatc                                                            <  2:5012798/100‑1 (MQ=255)
                                  tGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcatc                                                            <  1:150196/100‑1 (MQ=255)
                                  tGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcatc                                                            <  2:1660636/100‑1 (MQ=255)
                                  tGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAatcatc                                                            >  2:1582263/1‑100 (MQ=255)
                                   gTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAAtcatca                                                           <  1:3835427/100‑1 (MQ=255)
                                   gTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAAtcatca                                                           >  2:5066365/1‑100 (MQ=255)
                                       aGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAAc                                                       <  1:1654074/100‑1 (MQ=255)
                                        ggCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACg                                                      >  1:169447/1‑100 (MQ=255)
                                        ggCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACg                                                      >  1:1135568/1‑100 (MQ=255)
                                          cGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGaa                                                    <  2:2762666/100‑1 (MQ=255)
                                            aGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGaaaa                                                  <  2:4009689/100‑1 (MQ=255)
                                             gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                 <  2:2004059/100‑1 (MQ=255)
                                             gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                 <  1:1408127/100‑1 (MQ=255)
                                             gCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAg                                                 <  1:4787902/100‑1 (MQ=255)
                                               aaaaTCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGcc                                               <  1:2208768/100‑1 (MQ=255)
                                                aaaTCAGACTTCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGccc                                              >  1:1530563/1‑100 (MQ=255)
                                                aaaTCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGccc                                              >  2:4110978/1‑100 (MQ=255)
                                                aaaTCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGccc                                              >  2:2538792/1‑100 (MQ=255)
                                                 aaTCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGcccn                                             <  1:2235293/100‑2 (MQ=255)
                                                  aTCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCt                                            >  2:2920010/1‑100 (MQ=255)
                                                   tCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAACAGGACAAACGAAGCGAGAAAACTCAAAAACATCATAACGAAAAGCCCAtt                                           <  2:2408868/100‑1 (MQ=255)
                                                      gACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACt                                        >  2:4510711/1‑100 (MQ=255)
                                                       aCTGCTGCTAAATAAGCGCACGCGGTGATAGACCAACGCGCTGATCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACtt                                       <  1:4730840/100‑1 (MQ=255)
                                                       aCTGCTGAGAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACtt                                       >  2:636890/1‑100 (MQ=255)
                                                        ctgctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTg                                      >  1:2900084/1‑100 (MQ=255)
                                                         tgctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGGGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGt                                     <  2:5036814/100‑1 (MQ=255)
                                                          gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                    >  2:4421334/1‑100 (MQ=255)
                                                          gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                    <  2:819364/100‑1 (MQ=255)
                                                          gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                    >  1:249108/1‑100 (MQ=255)
                                                          gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                    >  1:5126836/1‑100 (MQ=255)
                                                          gctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTa                                    >  2:3505126/1‑100 (MQ=255)
                                                           gtgATAGATGTGCCCACACGGGGATAGAACACCGCTCTGTGCAGGGCAGCCTTACCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTag                                   <  2:4306008/99‑1 (MQ=255)
                                                           ctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTag                                   <  2:909137/100‑1 (MQ=255)
                                                           ctgATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTag                                   >  2:529428/1‑100 (MQ=255)
                                                             gATAAATAAGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTagga                                 >  1:1673869/1‑100 (MQ=255)
                                                               taaataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTaggagg                               >  2:1261359/1‑100 (MQ=255)
                                                                 aataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTc                             >  1:3495957/1‑100 (MQ=255)
                                                                 aataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTc                             <  2:3958618/100‑1 (MQ=255)
                                                                  ataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATACCGAAAAGCCCCTTACTTGTAGGAGGTCt                            >  2:3155285/1‑100 (MQ=255)
                                                                  ataaGCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCt                            <  1:4159859/100‑1 (MQ=255)
                                                                      gCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGAcc                        <  2:226255/100‑1 (MQ=255)
                                                                      gCTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGAcc                        <  1:1881218/100‑1 (MQ=255)
                                                                       cTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCa                       >  1:2272473/1‑100 (MQ=255)
                                                                       cTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCa                       >  2:27524/1‑100 (MQ=255)
                                                                       cTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCa                       >  2:2022120/1‑100 (MQ=255)
                                                                       cTCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCa                       >  2:4055173/1‑100 (MQ=255)
                                                                        tCACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCAc                      <  2:133954/100‑1 (MQ=255)
                                                                         cACGCGGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGAGAGAATACTCAAAATAATCATAACGAAAAGACCCTTACTTGTAGGAGGTCTGACCACt                     >  2:487509/1‑100 (MQ=255)
                                                                           cgcgGTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTtg                   <  2:386076/100‑1 (MQ=255)
                                                                               gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat               <  2:1443288/100‑1 (MQ=255)
                                                                               gTGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgat               <  1:1570906/100‑1 (MQ=255)
                                                                                tGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatg              >  1:3527520/1‑100 (MQ=255)
                                                                                tGATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGtgatg              <  1:2669901/100‑1 (MQ=255)
                                                                                 gATAGAACAACGCGCTGAGCATGACAACCATAGCGAGAATACTCAAAATCATGATAACGAAAAGACACTTACTTGTAGGCGGTCTGACCACTTGtgatga             >  2:1121575/1‑100 (MQ=255)
                                                                                 gATAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGTGGTCTGACCACTTGtgatga             <  1:4018530/100‑1 (MQ=255)
                                                                                  aTAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTgatgat            <  1:755875/100‑1 (MQ=255)
                                                                                   tAGAATAACGCGCTGAGCAGGACCACCGTAGCGAGAATACTCAAAGTCGTCTTATCGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGata           <  2:4459771/100‑1 (MQ=255)
                                                                                   tAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGata           <  1:1011990/100‑1 (MQ=255)
                                                                                   tAGAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGata           >  1:4202249/1‑100 (MQ=255)
                                                                                     gAACAACGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATg         >  1:4280264/1‑100 (MQ=255)
                                                                                      accaacGCGCTGAGCAGGACAGCCGTAGCGAGGATACTCAAAATCATCATAAGGAAAAGCTCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATgg        <  2:148518/98‑1 (MQ=255)
                                                                                       acaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGt       <  2:2383993/100‑1 (MQ=255)
                                                                                       acaacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGt       >  2:2091148/1‑100 (MQ=255)
                                                                                        caacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGtt      <  1:3096049/100‑1 (MQ=255)
                                                                                         aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg     >  1:3597849/1‑100 (MQ=255)
                                                                                         aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg     >  1:27162/1‑100 (MQ=255)
                                                                                         aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg     <  2:2680926/100‑1 (MQ=255)
                                                                                         aacGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTg     <  2:1641953/100‑1 (MQ=255)
                                                                                          acGCGCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGt    <  1:594124/100‑1 (MQ=255)
                                                                                           cgcgCTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTa   >  1:3981055/1‑100 (MQ=255)
                                                                                            gcgcTGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTAg  <  1:2568171/100‑1 (MQ=255)
                                                                                                |                                                                                               
ACGCCGACCACAGACCAGCAACGCCGAGGGCGGCTGTCCAGGCGAGCAAAATCAGACTGCTGATAAATAAGCTCACGCGGTGATAGAACAACGCGCCGAGCAGGACAACCGTAGCGAGAATACTCAAAATCATCATAACGAAAAGCCCCTTACTTGTAGGAGGTCTGACCACTTGTGATGATATGGTTGTAG  >  NC_000913/243168‑243359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A