Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I3 R1 53 201.3 10250884 92.1% 9441064 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 714,588 C→T A344V (GCC→GTC)  pgm → phosphoglucomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913714,5880CT97.9% 631.0 / ‑0.9 188A344V (GCC→GTC) pgmphosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (109/75);  total (112/76)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

CGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTT  >  NC_000913/714493‑714684
                                                                                               |                                                                                                
cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              >  1:3571600/1‑100 (MQ=255)
cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              <  2:519298/100‑1 (MQ=255)
cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              <  2:3534117/100‑1 (MQ=255)
cGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTgtcgtc                                                                                              >  1:261539/1‑100 (MQ=255)
  tCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCgg                                                                                            <  1:2235456/100‑1 (MQ=255)
   cACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGt                                                                                           >  2:3746902/1‑100 (MQ=255)
     cTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaa                                                                                         >  1:3988466/1‑100 (MQ=255)
     cTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaa                                                                                         >  2:343068/1‑100 (MQ=255)
      tCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaa                                                                                        <  1:1837289/100‑1 (MQ=255)
       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaaa                                                                                       >  2:1048461/1‑100 (MQ=255)
       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTaaaa                                                                                       >  2:4004270/1‑100 (MQ=255)
       ccGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTCTCGTCGGTaaaa                                                                                       <  1:4432424/100‑1 (MQ=255)
        cGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAAc                                                                                      >  1:1330876/1‑100 (MQ=255)
         ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACg                                                                                     >  2:2686502/1‑100 (MQ=255)
         ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACg                                                                                     >  2:2661649/1‑100 (MQ=255)
                ttGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGttt                                                                              <  2:4766847/100‑1 (MQ=255)
                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  2:1310137/100‑1 (MQ=255)
                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  1:1436606/100‑1 (MQ=255)
                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            >  2:348920/1‑100 (MQ=255)
                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            >  1:330455/1‑100 (MQ=255)
                  gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCa                                                                            <  1:2877358/100‑1 (MQ=255)
                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCCAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          >  2:4465868/1‑100 (MQ=255)
                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          <  1:4740948/100‑1 (MQ=255)
                    tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATc                                                                          >  2:1747774/1‑100 (MQ=255)
                      aaTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGCTGTTGTCGTCGGTAAAACGCTGGTTTCATCTg                                                                        >  2:3617511/1‑100 (MQ=255)
                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATcgca                                                                       >  2:2320349/1‑97 (MQ=255)
                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       <  1:307810/100‑1 (MQ=255)
                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       >  1:5105222/1‑100 (MQ=255)
                       aTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGc                                                                       <  1:641407/100‑1 (MQ=255)
                          cGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  2:2070948/100‑1 (MQ=255)
                          cGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgac                                                                    >  2:3001730/1‑99 (MQ=255)
                          cGAACCACTACCTGGCGGTGGCAATCAATCACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  1:4707521/100‑1 (MQ=255)
                          cGAACCACGACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCgat                                                                    <  2:3555793/100‑1 (MQ=255)
                              ccACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATc                                                                >  1:1741231/1‑100 (MQ=255)
                              ccACTACCTGGCGGTGGCAATCAATAAACTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTACAACGCTGGTTTCATCTGCGATGATc                                                                >  2:186430/1‑100 (MQ=255)
                               cACTACCTGGCGGTGGCGATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTTGGTAAAACGCTGGTTTCTTCTGCGATGATCg                                                               >  2:314392/1‑100 (MQ=255)
                               cACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCg                                                               >  1:1886861/1‑100 (MQ=255)
                                aCTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGa                                                              >  1:444604/1‑100 (MQ=255)
                                aCTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGa                                                              >  2:2383776/1‑100 (MQ=255)
                                  tACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGAcc                                                            <  2:7673/100‑1 (MQ=255)
                                   aCCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCg                                                           >  1:4316401/1‑100 (MQ=255)
                                     cTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCgtg                                                         >  1:2016028/1‑100 (MQ=255)
                                      tGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCgtgt                                                        <  2:3843096/100‑1 (MQ=255)
                                       ggCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGtgtg                                                       <  2:2631735/100‑1 (MQ=255)
                                        gCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTgg                                                      <  1:4668702/100‑1 (MQ=255)
                                         cGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGt                                                     <  1:2717205/100‑1 (MQ=255)
                                          ggTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTc                                                    >  1:774701/1‑100 (MQ=255)
                                            tGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCaa                                                  >  1:5111601/1‑100 (MQ=255)
                                             ggCAATCAATTACCTGTTCCAGCCTCCTCCGCACTGGGGAAACGATGTTGTCCTCCGTAACACGCTGGTTTCATCTGCGATGATCGACCATGCGGTccag                                                 <  2:1007216/100‑4 (MQ=255)
                                             ggCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAAc                                                 >  1:4342406/1‑100 (MQ=255)
                                               caatcaatTACCTGTTCCAGCATTGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  1:1120658/1‑100 (MQ=255)
                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAACGATGTTGTCGTCGGTAAAATGCCGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:2759343/1‑100 (MQ=255)
                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:29622/1‑100 (MQ=255)
                                               caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGa                                               >  2:2243648/1‑100 (MQ=255)
                                                aatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGAc                                              >  2:1644128/1‑100 (MQ=255)
                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             >  1:1559321/1‑100 (MQ=255)
                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             <  1:1056484/100‑1 (MQ=255)
                                                 atcaatTACCTGTTCCAGCATCGTCCGCAGTGCGGCAAAGCTGTTGTCGTCGCTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACt                                             >  2:4989285/1‑100 (MQ=255)
                                                   caatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTg                                           <  1:2500018/100‑1 (MQ=255)
                                                    aatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTgg                                          >  1:1463658/1‑100 (MQ=255)
                                                      ttACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGc                                        <  2:2529218/100‑1 (MQ=255)
                                                      ttACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGc                                        <  1:972055/100‑1 (MQ=255)
                                                        aCCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCg                                      <  1:2073251/100‑1 (MQ=255)
                                                         ccTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTGATCTTCGATGATCGACCGTGTGGTCAACGACTTGGGCCGt                                     <  2:4956025/100‑1 (MQ=255)
                                                          cTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTa                                    >  1:4929178/1‑100 (MQ=255)
                                                           tGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaa                                   >  2:3928754/1‑100 (MQ=255)
                                                            gTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaaa                                  >  1:1112429/1‑100 (MQ=255)
                                                              tCCAGGATCGTCCGCAGTGGGGGAAAGATGTTGTCGTCGGTAAAACGCGGGTTTCATCTGCTATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                <  2:2079115/100‑1 (MQ=255)
                                                              nccAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                >  2:4663889/2‑100 (MQ=255)
                                                               ccAGCATCGTCCGCAGTGGGGCAAGGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTg                               >  2:3346703/1‑100 (MQ=255)
                                                               ccAGCATCGTCCGCAGTGGGCCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTg                               >  1:4033774/1‑100 (MQ=255)
                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:2402383/1‑100 (MQ=255)
                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:2078636/1‑100 (MQ=255)
                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              >  2:4416243/1‑100 (MQ=255)
                                                                cAGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                              <  1:1803204/100‑1 (MQ=255)
                                                                 aGCATCGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGt                             <  1:1016262/100‑1 (MQ=255)
                                                                      cGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAg                        <  1:2740634/100‑1 (MQ=255)
                                                                      cGTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAg                        >  2:2836118/1‑100 (MQ=255)
                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAGACTGGTAGAAGt                       >  2:4839347/1‑100 (MQ=255)
                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  1:1246118/1‑100 (MQ=255)
                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  1:410305/1‑100 (MQ=255)
                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  2:1307944/1‑100 (MQ=255)
                                                                       gTCCGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGt                       >  2:388063/1‑100 (MQ=255)
                                                                         ccGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTcc                     >  2:4563514/1‑100 (MQ=255)
                                                                         ccGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTcc                     >  1:102945/1‑100 (MQ=255)
                                                                          cGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTccc                    <  2:1990935/100‑1 (MQ=255)
                                                                          cGCAGTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTccc                    >  1:4602307/1‑100 (MQ=255)
                                                                              gTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTa                <  1:4350948/100‑1 (MQ=255)
                                                                              gTGGGGCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTa                >  1:2814749/1‑100 (MQ=255)
                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              >  2:1570490/1‑100 (MQ=255)
                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              >  1:4811877/1‑100 (MQ=255)
                                                                                ggggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAgg              <  1:3657434/100‑1 (MQ=255)
                                                                                 gggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGt             >  1:4272086/1‑100 (MQ=255)
                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  2:3752858/1‑100 (MQ=255)
                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  2:1409999/1‑100 (MQ=255)
                                                                                  ggCAAAGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGtt            >  1:32647/1‑100 (MQ=255)
                                                                                       aGATGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaaa       >  1:2641175/1‑100 (MQ=255)
                                                                                         aTGTTGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATg     <  1:2667098/100‑1 (MQ=255)
                                                                                          tgttgtCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATgg    <  2:4258168/100‑1 (MQ=255)
                                                                                            ttGTCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGtt  >  2:86889/1‑100 (MQ=255)
                                                                                               |                                                                                                
CGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTT  >  NC_000913/714493‑714684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A