Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A10 F418 I1 R1 | 19 | 54.4 | 1474142 | 94.2% | 1388641 | 177.8 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 451,881 | A→G | intergenic (‑271/+189) | cyoA ← / ← ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 451,881 | 0 | A | G | 100.0% | 159.6 / NA | 42 | intergenic (‑271/+189) | cyoA/ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22) | |||||||||||
TGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAA > NC_000913/451653‑452103 | tgtgGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGtg > 2:333736/1‑239 (MQ=255) tGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATc > 2:532181/1‑239 (MQ=255) gTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTgg < 1:243913/185‑1 (MQ=255) gTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTgg > 2:243913/1‑185 (MQ=255) tttGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGgcgc > 1:117362/1‑239 (MQ=255) ccAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAAtt > 2:435860/1‑239 (MQ=255) tCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTcc < 1:435860/237‑1 (MQ=255) tGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGtga > 1:215792/1‑156 (MQ=255) tGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGtga < 2:215792/156‑1 (MQ=255) tGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGcc > 1:361031/1‑239 (MQ=255) gTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACaaa > 1:241225/1‑239 (MQ=255) ttttAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATta < 1:532181/239‑1 (MQ=255) agaagCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAg < 2:241225/239‑1 (MQ=255) ttGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGaa < 2:616098/150‑1 (MQ=255) ttGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGaa > 1:616098/1‑150 (MQ=255) gCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGAt < 1:239614/239‑1 (MQ=255) gCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGAt < 1:16509/239‑1 (MQ=255) gCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGaa > 1:692151/1‑140 (MQ=255) gCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGaa < 2:692151/140‑1 (MQ=255) gCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGATGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGTCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGtttattt > 2:424925/1‑239 (MQ=255) ggggAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAg < 1:111562/207‑1 (MQ=255) ggggAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAg > 2:111562/1‑207 (MQ=255) gTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACg > 2:38790/1‑236 (MQ=255) gTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACg < 1:38790/236‑1 (MQ=255) gCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGa < 2:117362/238‑1 (MQ=255) gCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGa < 2:361031/238‑1 (MQ=255) tGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTg < 2:58743/239‑1 (MQ=255) cTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGATGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATtacta < 1:424925/237‑1 (MQ=255) ggcAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAg > 1:301892/1‑182 (MQ=255) ggcAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAg < 2:301892/182‑1 (MQ=255) ggTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATAttt < 1:333736/239‑1 (MQ=255) aCCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGa < 2:519750/207‑1 (MQ=255) aCCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGa > 1:519750/1‑207 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATgag > 1:511212/1‑60 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATgag < 2:511212/60‑1 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATta > 2:123358/1‑159 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATta < 1:123358/159‑1 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGtttat > 1:28947/1‑180 (MQ=255) tgtgAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGtttat < 2:28947/180‑1 (MQ=255) aatTATCATTAGAGTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATaa > 1:728800/1‑239 (MQ=255) agagTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATtacta > 1:716472/1‑174 (MQ=255) agagTGGCAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATtacta < 2:716472/174‑1 (MQ=255) | TGTGGGCATTTTACGGGAAGGTTACATTACTGTAAACATGATTAAATTCCTGTTACTTGGATTTGGCTGCCAGGTCACATATGTTGCAAAACATATCAGGTTGATTTTAAGAAGCTGTTGCAAAAGGGCGTTATAACAAAAGGGGAGTGCTGAAGGAGTCTGGGCGGGCAATTGGTATAACCAATGTGAAATAAAACAATTATTTAACAAATAATTATCATTAGAGTGACAACTGGGTGATGAGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAATAGAATTTATAAATGGCGCCAATAATTTCCAAACGCTAAGCCGCACAAAAGAACAAATATTAATAAGCGATACTGATGTTTATTTAGCCGTAATAATTACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCGTTTTTCGTAGCGCCAGATAA > NC_000913/451653‑452103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |