Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F121 I1 R1 14 130.3 2998436 96.1% 2881496 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 587,957 G→T A621S (GCG→TCG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE587,9570GT82.8% 276.6 / 70.3 128A621S (GCG→TCG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (7/15);  new base T (39/67);  total (46/82)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 130 total aligned reads displayed.

ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTGCAG  >  USA300TCH1516_ALE/587818‑588092
                                                                                                                                           |                                                                                                                                       
acaAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCagcagc                                                                                                                                        >  2:660705/1‑141 (MQ=255)
   aGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGAc                                                                                                                                     <  4:332651/141‑1 (MQ=255)
     cAAAATTTAAATTAGAAGAAAAAGGTCCTTTCATTGAAGAATAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAg                                                                                                                                   <  3:287014/141‑1 (MQ=255)
       aaCTCTAAAATAGAAAAAAAAACTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTAAAAAAAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCa                                                                                                                                 <  2:360257/141‑1 (MQ=255)
           ctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTa                                                                                                                             >  2:809952/1‑141 (MQ=255)
           ctAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTa                                                                                                                             >  1:787472/1‑141 (MQ=255)
             aaaTTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGACGTATCGCCGAAGCAAGTTGTCTCAGCAGCGACAGCATGTact                                                                                                                           >  2:186404/1‑139 (MQ=255)
               aTTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTcc                                                                                                                         >  2:138040/1‑141 (MQ=255)
                 tAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCAt                                                                                                                       <  1:660705/141‑1 (MQ=255)
                   gATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTc                                                                                                                     <  3:222602/141‑1 (MQ=255)
                   gATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTc                                                                                                                     >  2:836538/1‑141 (MQ=255)
                     tGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCtt                                                                                                                   <  1:137111/141‑1 (MQ=255)
                     tGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCtt                                                                                                                   <  1:840006/141‑1 (MQ=255)
                      gAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTa                                                                                                                  <  2:484127/141‑1 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAg                                                                                                                 >  2:875552/1‑141 (MQ=255)
                       aaaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAg                                                                                                                 >  1:978601/1‑141 (MQ=255)
                        aaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGa                                                                                                                <  1:1025732/141‑1 (MQ=255)
                        aaaTGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGa                                                                                                                <  1:341182/141‑1 (MQ=255)
                           tGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTAAGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGaaaa                                                                                                             <  2:744791/141‑1 (MQ=255)
                              tCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatga                                                                                                          <  2:489368/141‑1 (MQ=255)
                               cGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAatgat                                                                                                         >  1:882103/1‑141 (MQ=255)
                                gTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAatgatg                                                                                                        <  1:480594/141‑1 (MQ=255)
                                 tttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAAtgatga                                                                                                       >  1:110801/1‑141 (MQ=255)
                                  ttCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTGAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGAc                                                                                                      >  1:283370/1‑141 (MQ=255)
                                   tCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACt                                                                                                     <  3:351985/141‑1 (MQ=255)
                                   tCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTAAAAAAAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACt                                                                                                     <  4:118441/141‑1 (MQ=255)
                                     aTGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCa                                                                                                   >  2:484792/1‑141 (MQ=255)
                                       ggATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCaaa                                                                                                 >  2:561223/1‑141 (MQ=255)
                                        gatgatGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGATAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAAc                                                                                                <  2:1016857/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                          <  1:402793/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                          <  2:761475/141‑1 (MQ=255)
                                              gaAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCa                                                                                          <  1:867956/141‑1 (MQ=255)
                                               aaGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAt                                                                                         <  2:911523/141‑1 (MQ=255)
                                               aaGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCCAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAt                                                                                         >  2:899496/1‑141 (MQ=255)
                                                aGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAtt                                                                                        <  1:689921/141‑1 (MQ=255)
                                                aGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCAtt                                                                                        <  2:527937/141‑1 (MQ=255)
                                                 gTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTg                                                                                       <  1:601915/141‑1 (MQ=255)
                                                    gTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATg                                                                                    <  2:106774/141‑1 (MQ=255)
                                                      aTGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATggg                                                                                  >  1:105275/1‑141 (MQ=255)
                                                      aTGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATggg                                                                                  <  2:258332/141‑1 (MQ=255)
                                                          cGTTTCCGTGGTAACAATACAGTTATGGCTAAAGCAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCg                                                                              <  1:968914/141‑1 (MQ=255)
                                                          cGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCg                                                                              <  2:391197/141‑1 (MQ=255)
                                                              tCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACa                                                                          <  4:401094/141‑1 (MQ=255)
                                                              tCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACa                                                                          <  2:520387/141‑1 (MQ=255)
                                                               ccGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACAt                                                                         <  2:295547/141‑1 (MQ=255)
                                                                cGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATg                                                                        >  1:370572/1‑141 (MQ=255)
                                                                 gTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGc                                                                       >  3:417429/1‑141 (MQ=255)
                                                                 gTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGc                                                                       <  1:671412/141‑1 (MQ=255)
                                                                  tGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCa                                                                      <  1:203014/141‑1 (MQ=255)
                                                                    gTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAAc                                                                    >  2:794560/1‑141 (MQ=255)
                                                                     tAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACg                                                                   <  1:704889/141‑1 (MQ=255)
                                                                     tAACAATACAGTTATGGCGAAAAAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACg                                                                   <  4:210489/141‑1 (MQ=255)
                                                                      aaCAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                                  <  1:585949/141‑1 (MQ=255)
                                                                        cAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                                <  4:50800/141‑1 (MQ=255)
                                                                          aTACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAg                                                              >  2:182815/1‑141 (MQ=255)
                                                                            aCAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCa                                                            >  2:842239/1‑141 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                           <  1:343636/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                           <  2:890298/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATGCCATTCGTAGAAAATGAGGACTCAAACCGGGCATTGATGTGTGCGAACATGCAACGTCAAGCAg                                                           <  4:37785/141‑1 (MQ=255)
                                                                             cAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAg                                                           <  4:312979/141‑1 (MQ=255)
                                                                              aGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGt                                                          <  2:874498/141‑1 (MQ=255)
                                                                               gTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTg                                                         >  2:386516/1‑141 (MQ=255)
                                                                                 tATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGcc                                                       <  1:638118/141‑1 (MQ=255)
                                                                                   tGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCtt                                                     >  2:802494/1‑141 (MQ=255)
                                                                                    ggCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCttt                                                    >  3:64498/1‑141 (MQ=255)
                                                                                       tAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgat                                                 >  2:9740/1‑141 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                <  1:135088/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                <  1:644561/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                <  1:809851/141‑1 (MQ=255)
                                                                                        aaaGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTtgatg                                                <  1:300818/141‑1 (MQ=255)
                                                                                              aaaaTTTATGATATCGATGTATCGCCGAAGCAAGGTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAATACTATTGTTCAATGCTTTTATATATTTTTGCGAACATGCAACTTCAGGCAGTGCCTTTTATGAATCCa                                          <  2:833385/141‑1 (MQ=255)
                                                                                               aaaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAg                                         >  1:872075/1‑141 (MQ=255)
                                                                                                aaTGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGa                                        <  4:256117/141‑1 (MQ=255)
                                                                                                  tGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAg                                      <  2:110801/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                    >  2:121914/1‑141 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                    <  1:234235/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                    <  1:820782/141‑1 (MQ=255)
                                                                                                    gATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCa                                    >  4:269565/1‑141 (MQ=255)
                                                                                                     aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAc                                   >  4:239162/1‑141 (MQ=255)
                                                                                                     aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCAATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAc                                   <  2:207364/141‑1 (MQ=255)
                                                                                                     aTTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCAc                                   <  3:171549/141‑1 (MQ=255)
                                                                                                         tatGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAtt                               <  1:279124/141‑1 (MQ=255)
                                                                                                          atGGATGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAttt                              <  1:291729/141‑1 (MQ=255)
                                                                                                          atGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCAttt                              >  1:136837/1‑141 (MQ=255)
                                                                                                             gATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATttgtt                           <  3:36813/141‑1 (MQ=255)
                                                                                                               tGTATCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGGAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTgg                         >  1:532244/1‑141 (MQ=255)
                                                                                                                   tCGCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACa                     >  3:120252/1‑141 (MQ=255)
                                                                                                                    cGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAg                    <  1:101897/141‑1 (MQ=255)
                                                                                                                     gCCGAAGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAgg                   >  1:372211/1‑141 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg              <  1:228582/141‑1 (MQ=255)
                                                                                                                          aGCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATgg              <  2:419259/141‑1 (MQ=255)
                                                                                                                           gCAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGa             >  2:281545/1‑141 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa            >  4:202577/1‑141 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGaa            >  4:372514/1‑141 (MQ=255)
                                                                                                                            cAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTTATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGCAGCACCATTTGTTGGTACAGGTATGGaa            <  4:429478/141‑1 (MQ=255)
                                                                                                                              aGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAacc          >  2:608060/1‑140 (MQ=255)
                                                                                                                              aGTTGTTTCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAaca          <  3:76467/141‑1 (MQ=255)
                                                                                                                                ttgttTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACg        <  1:263909/141‑1 (MQ=255)
                                                                                                                                    ttCAGCATCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTgc    >  4:377692/1‑141 (MQ=255)
                                                                                                                                      cagcagCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTgcag  <  3:184579/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
ACAAGCAAACTCTAAATTAGATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACAGTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCAGCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCGAACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGTATGGAACACGTTGCAG  >  USA300TCH1516_ALE/587818‑588092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A