Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A24 F2 I0 R1 35 39.7 777878 96.9% 753763 248.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATC  >  NC_000913/4296143‑4296676
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                       
ATAAGGAGTTGACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACC                                                                                                                                                                                                                                             <  M01186:120:000000000‑A992N:1:2106:22282:8961/301‑1 (MQ=5)
    GGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCG                                                                                                                                                                                                                                            <  M01186:120:000000000‑A992N:1:1101:19199:10973/298‑1 (MQ=3)
            cgcagcgttctACATCACCGCCTGATGCGACTTTTGCCGCGTCTTATCATGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGGTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTT                                                                                                                                                                                                                                 <  M01186:120:000000000‑A992N:1:2102:25940:11413/290‑1 (MQ=12)
                 CATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCCTGCCGCGTCTGTTCAGGCCTATCTTACCCGTTGGTTAATTTTTCAA                                                                                                                                                                                                                            >  M01186:120:000000000‑A992N:1:2102:22094:6111/1‑301 (MQ=24)
                               GCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGGGTTTTATCAGGACTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACAC                                                                                                                                                                                                               >  M01186:120:000000000‑A992N:1:1101:4633:7880/1‑300 (MQ=22)
                                    ttgcgtcgtttgcCGCGTCTTATCAAGCCGACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATC                                                                                                                                                                                                         <  M01186:120:000000000‑A992N:1:2114:20089:5322/288‑1 (MQ=54)
                                         tctattgcggtgttTTATCAGGCCTACGCCAGGCAGCTCAATAGGCTGATTTAGCTTGATTTTGTAGTGCGGATAAGGCGTTTATGACGCATCCGACATCAACGCCTGAGGCGACTCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAC                                                                                                                                                                                                     <  M01186:120:000000000‑A992N:1:2116:16466:5600/286‑1 (MQ=53)
                                         acgccttcagccgctgatcaggcctacgccagacagcgcattatccttatttagcttgagttgttacgccgggtgcGGCGTTTATGCCGCCTCCTATACCAAAGCCTGCTGCCACGCTTACCGCGTCTTGTCAGGCTTACGCCAGCCAGCTCAATCGCCTGATTTATCGTGATGTTTTAGGTCGGATAAGGCGTGTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTTGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAC                                                                                                                                                                                                     <  M01186:120:000000000‑A992N:1:1116:21113:14828/224‑1 (MQ=48)
                                         CCGCTGAACGCGTCTTATGAGGCCTAGGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACC                                                                                                                                                                                                    <  M01186:120:000000000‑A992N:1:2102:24936:12078/301‑1 (MQ=60)
                                                CCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAT                                                                                                                                                                                             <  M01186:120:000000000‑A992N:1:1104:18734:12614/301‑1 (MQ=60)
                                                             GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGACCACGCCAGACAGCGCAATAGCCTGATTTAGCATGTTTTTGTAGGTCGGATAAGGCGCTTACGCCACATCCGACATCAATGCCTGATGCGACCCATGCCGCCTCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTAAAACTCTTCACACCCGGTATCAAACCCTACCATACAGCgccgatt                                                                                                                                                                                 >  M01186:120:000000000‑A992N:1:1103:9922:20789/1‑293 (MQ=60)
                                                             GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCCTCACACCCGGTATTAAACCCTACCATACAGATCAGGTC                                                                                                                                                                                 >  M01186:120:000000000‑A992N:1:1104:14618:3808/1‑300 (MQ=60)
                                                                 tactcagGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                                                                                                                                                                            <  M01186:120:000000000‑A992N:1:1103:15714:7789/294‑1 (MQ=60)
                                                                  ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGTAGCTTGCCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA                                                                                                                                                                           >  M01186:120:000000000‑A992N:1:2103:8518:16187/1‑301 (MQ=60)
                                                                            GCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGCTCAGTTTCAACCAATGCAGCGC                                                                                                                                                                  >  M01186:120:000000000‑A992N:1:1104:19838:11257/1‑300 (MQ=60)
                                                                                      CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTACCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCAGCCACTTTTGCTTGT                                                                                                                                                       >  M01186:120:000000000‑A992N:1:2111:28608:9373/1‑301 (MQ=60)
                                                                                        aattgcGCGTGATTTTGCAGGTCGGATAATGCGGTTATGCCGCATCCGACATCAACGCCGGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCTACGCTTGCCGCGTCTTATAAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC                                                                                                                                                     <  M01186:120:000000000‑A992N:1:1108:20328:6322/295‑1 (MQ=60)
                                                                                                   ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTAGCTTAACCGTTGGTTAATTTTTAAAACTCTTCACCCCCGGTATCCAACCCTTACCTACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAA                                                                                                                                          >  M01186:120:000000000‑A992N:1:1111:10266:14329/1‑301 (MQ=60)
                                                                                                   ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAA                                                                                                                                          >  M01186:120:000000000‑A992N:1:1107:13374:10252/1‑301 (MQ=60)
                                                                                                                         TTTATGCCGCATCCGACATCAACGCGTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCGGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTGTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAC                                                                                                                    <  M01186:120:000000000‑A992N:1:2114:27512:12898/301‑1 (MQ=60)
                                                                                                                                                     ATGCGACGCGTAACGGTCCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAC                                                                                        <  M01186:120:000000000‑A992N:1:2101:26104:10749/301‑1 (MQ=60)
                                                                                                                                                        CGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTACCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCTTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCG                                                                                      <  M01186:120:000000000‑A992N:1:2109:23628:19843/300‑1 (MQ=60)
                                                                                                                                                           CGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAACCGACATCAATGCCTGCTGCGACGCTTGCCGTGTCTTATCCGGCCAATATTAACCGTTGGTTAATTTTTCAAACCCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCACCCAGTGCAGCGCCTTTTGCTTGTTATTATCGCTAAAACCTTCTTCACCGTTTATGAAGATCATTCCCGCCCAGTACTCGGATCAACCGGTTT                                                                                  >  M01186:120:000000000‑A992N:1:2103:7664:10505/1‑301 (MQ=60)
                                                                                                                                                            GCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTCACACCCGGTATCAAACC                                                                                                                                                                                                    >  M01186:120:000000000‑A992N:1:2118:17693:17479/1‑186 (MQ=60)
                                                                                                                                                             CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAACCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGG                                                                                >  M01186:120:000000000‑A992N:1:2103:13750:15227/1‑301 (MQ=60)
                                                                                                                                                                AACGCGTCTTATCGGGCTGACGCCAGCAAGCGCAATAGCCTGATTTGGCGTGATTTTGTAGATCTGAGATGGCGTTTACGCCGCAACCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAA                                                                             <  M01186:120:000000000‑A992N:1:1103:11917:2666/301‑1 (MQ=60)
                                                                                                                                                                    CGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACTGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT                                                                                                                                                          >  M01186:120:000000000‑A992N:1:2102:19916:12253/1‑220 (MQ=60)
                                                                                                                                                                         TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAG                                                                    >  M01186:120:000000000‑A992N:1:2107:18790:7236/1‑301 (MQ=60)
                                                                                                                                                                         TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCG                                                                                      <  M01186:120:000000000‑A992N:1:2101:14221:13868/283‑1 (MQ=60)
                                                                                                                                                                               GCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCG                                                                                      <  M01186:120:000000000‑A992N:1:1106:22248:14291/277‑1 (MQ=60)
                                                                                                                                                                                CCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCCGATAAGGCGGTTACGCCGCATCCGACATCAATGCCTGATGCTACGCTTGCCGTGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGT                                                              <  M01186:120:000000000‑A992N:1:2112:14096:19247/300‑1 (MQ=60)
                                                                                                                                                                                   ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAACACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACCCGGAATAACCGGTTCGGTAACTTGAAGAGCTGCGTTTGA                                                          >  M01186:120:000000000‑A992N:1:2108:24997:18043/1‑301 (MQ=60)
                                                                                                                                                                                   ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGA                                                          >  M01186:120:000000000‑A992N:1:1113:20760:9981/1‑301 (MQ=60)
                                                                                                                                                                                   ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT                                                                                                                                                          <  M01186:120:000000000‑A992N:1:2108:18290:5763/205‑1 (MQ=60)
                                                                                                                                                                                   ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGCTAAAACCTTCTTCACCGTTTCAGAACATCATTCCCGCACAGTACTCGGAATAACCGGTTCGGAGAATTGCAGAGCTGCGTTTGA                                                          >  M01186:120:000000000‑A992N:1:2102:23584:19511/1‑301 (MQ=60)
                                                                                                                                                                                             GCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGCCGTTTACGCCGCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCG                                                                                      <  M01186:120:000000000‑A992N:1:2108:18793:3974/263‑1 (MQ=60)
                                                                                                                                                                                              CGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCCTTTAAGAACATCATTCCCGCCCAGTAT                                                                                                  >  M01186:120:000000000‑A992N:1:1112:21286:19265/1‑250 (MQ=60)
                                                                                                                                                                                                     GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAC                                                                                                                               <  M01186:120:000000000‑A992N:1:1115:16745:16901/214‑1 (MQ=60)
                                                                                                                                                                                                     GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCtgtcccttataccaatctccgagcccacgaaactcctctacatctcgtatgccgtcttctgcctt                                        >  M01186:120:000000000‑A992N:1:2103:24954:12741/1‑236 (MQ=60)
                                                                                                                                                                                                                    ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATCAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGACATACCAGGTTGCCTTGTCA                                      >  M01186:120:000000000‑A992N:1:2101:14299:8264/1‑288 (MQ=60)
                                                                                                                                                                                                                                           ttacgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCGTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATC  <  M01186:120:000000000‑A992N:1:2106:14535:6942/296‑1 (MQ=60)
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ATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGTTGGCGTAAATC  >  NC_000913/4296143‑4296676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑