Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 43 55.2 1410130 81.3% 1146435 215.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_000913 4,001,645 Δ5 bp 93.9% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 40016443 (0.060)46 (0.930) 36/408 NT 93.9% coding (219/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913 4001650 = 3 (0.060)coding (225/951 nt) corA magnesium/nickel/cobalt transporter

TGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001427‑4001644
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAA  >  NC_000913/4001650‑4001873
                                                                                                                                                                                                                                                                                                                                                                                                                                                          
aGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTT                                                                                                                                                                                                                         <  1:69433/226‑1
      GCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCCTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTT                                                                                                                                                                                                                  >  1:554946/1‑228
         CATTGCAACGGGAAAATAACCGAATGGCCCGGCTGGAAGTCGAAGAGTCAGACCCCCTTGGAAAGGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGA                                                                                                                                                                                                                <  1:310370/227‑1
           TTTCAAAGGGAAAATACCCGAAGTAGCCGGCGGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGGATGGATTGATCTTGGCGAACCGGACGACGACGAGCGACGGCGCGTACAATCTGAAGTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGA                                                                                                                                                                                                             <  2:580723/228‑1
               AACTTGAAAATAACCGCCGGACCCGGCGGGAATTCGAAGAGTCACAACCCCTTGGAAATGCAGGATGGATTGATCTTGGCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCG                                                                                                                                                                                                         <  2:556716/228‑1
                        ATAACCGACTGACACGGCTGGAAGTCGAAGAGTCAAAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAC                                                                                                                                                                                                <  1:572134/228‑1
                         taaccgatctcCCCGGCTGGAAGTCGAAGAGTAAACACCCCTTGAAAAGGCAGTAGGGATTGATCTTGGCGAACCGGGCGACGACGAGCTACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACG                                                                                                                                                                                               <  1:527921/217‑1
                         TAACCGGCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACG                                                                                                                                                                                               <  1:88534/228‑1
                           accacgtgtCCCGGTTGGAAGTCGAAGGGGACCAACCCCTTGGAAAAGCAGTGTGGATTGATGTTGTCGAACCGGACGACGCCGGGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCC                                                                                                                                                                                             <  2:84683/219‑1
                              gtCTGACCCGGCTGGAAGGCGAAGAGGCCCAACCCCTTGGAAAGTCAGTATGGATTGATTTTGGCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                                                                                                                          <  1:129724/226‑1
                              ctCTGGCCCGGTTGGAATTTGAAGAGTCCAACCCCCTTGGAAATGCAGTATGGATTTATCTTGTCGAACCGGACGACGACCAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGCCGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                                                                                                                          <  2:142363/226‑1
                               gCTGGCCCGGATTGAAGTCGAAGAGTCACAACCCCGTGTAAAGGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTGGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGT                                                                                                                                                                                          <  1:304290/226‑1
                                   ACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTTAAGATTCGGAAGATCACGC                                                                                                                                                                                              >  1:84683/1‑219
                                        GCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGCCGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGCCATCGAAGCATCGGCACGGTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGGAACTCCcccg                                                                                                                                                                                >  2:499158/1‑224
                                         CTGGAAGTCGGAGAGTCACAACCGCTGGTAAATGGAGTATGGATGGGTCTTGTCGAACCGGACGCCGACGAGCGACTGGGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGTAAGATCACGCCGGTAACTCCACTGT                                                                                                                                                                               <  1:248760/228‑1
                                                    agggtccaAACCCCTGGGAAGTGCAGTCTGGATTGATCTTGCCGAACCGGACGCCGACGCGCGACTGCGCGTACAATCGGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTTGAAGACATCGAAGCATCGGCTCGTTTCTGTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATTCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACC                                                                                                                                                                     <  2:560412/219‑1
                                                                 tgTGTAATTTCGGTAGGGTTTGATCTTGGCGAACCGGACGACGCAGGGCGCCGTCGGGTACAATCTGAACTTGGCACGGGCCTGGCATCCGGCCCGGAACTGGAAGCCATCCAAGCATCGCCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCG                                                                                                                                                       <  2:176191/226‑1
                                                                  TTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGCGCCTGGCACCCCGCCCGGAACTGGACGACATCGAAGCATCGGCACGTTTCTTTGACGACGACGACGTCCTGCATCTCCTTCTTCTCCTTTGATGATGCGGACGATCAGACCGGGAACCCCACTGTGCCATTTACCATCCGGGATGGaggg                                                                                                                                                      >  2:335794/1‑224
                                                                  TTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGc                                                                                                                                                         >  1:274169/1‑224
                                                                   TGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGACAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGCAGCATCGGCACGTTTCTTTGCAGACGACTACGGCCTGCATCTCCCTCTTCTTCTTTTAAGATGCGGAAGATCACGCCGGTAACTACACCGTGGCCTTTACCATCCGTGAAGGTAGTa                                                                                                                                                     >  2:25214/1‑227
                                                                        ATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGTTCGGCTGTTT                                                                                                                                                >  1:352474/1‑228
                                                                          GCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTCCACCTCCTTCTCCTCCTTTGCAGATGCGGCAGATCACGCCGGAACCTCCCCCGTGGCCTTTACCATCCGTGATGTTCccccttttac                                                                                                                                              >  2:623844/1‑218
                                                                              TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCT                                                                                                                                           >  1:167479/1‑227
                                                                              TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGTATGGTCGTCTGTTTctgtc                                                                                                                                           >  1:62599/1‑222
                                                                                        TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGCCGTCTGTTTACTCTGCCTGCGCGgt                                                                                                                                >  1:371572/1‑226
                                                                                                      ACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTT                                                                                                                  <  1:499158/228‑1
                                                                                                          CGACGCGCGGCCGGGCTTACAAGTCGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACTTCGAAGCATCGGCACGTTTCTTGGAAGACGACGTCGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                                                                                                              <  2:480433/228‑1
                                                                                                                   ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACC                                                                                                                                                                     >  1:286124/1‑164
                                                                                                                         CGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTTGTTTGTATCGTAgcgggc                                                                                               >  1:561838/1‑222
                                                                                                                                    AACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCT                                                                                                                                                                                                                          >  1:500835/1‑94
                                                                                                                                     ACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTT                                                                                                                                                                                                                         <  2:500835/94‑1
                                                                                                                                        gggccggacgCTGGCAACCCCCCCCGAACTGGCAGGCATCTAAGCATCGGCACGTTTCTTTGAAGACGACGCTGGCCTGCATCTCCTTCTTCTCCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTATGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGA                                                                                <  1:335912/218‑1
                                                                                                                                          GCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGATGGTCGTCTGTTTACTCTGCGTAGGCGTGAACTGCCCCCTTTTGGGCTGTTTCTTATGCGGGCCCGGAGCagatcgtgg                                                                              >  2:123760/1‑219
                                                                                                                                                    GGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAATCTGCGTGAGCGTGACTTGCCCGCTTTTCGCTTGAATCGTATGCGTGCCCGTCGCCAGTCGATGGTAGACGGTA                                                                    >  1:429719/1‑228
                                                                                                                                                      cagCCCGCCCGGACCTGGAGGCCATGGAAGAATCGGCACTTTTCTTTGGAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAA                                                                   <  1:30302/224‑1
                                                                                                                                                            GCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCG                                                                                                                <  1:216862/176‑1
                                                                                                                                                                            CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTTCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGCCGGTAACGCCTCCGAGTTGCTGCTGGtac                                            >  2:210956/1‑225
                                                                                                                                                                            CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGA                                                                                <  1:536496/192‑1
                                                                                                                                                                            CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGA                                                                                >  2:536496/1‑192
                                                                                                                                                                               CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTTACTCGTATGCTTGCCCGTAGCCAGTCGATGGTAGACGGTAACCCCCACGGGTTGGCGTGGGATCTGg                                         >  2:282284/1‑227
                                                                                                                                                                               CGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGC                                                                                                  >  2:232342/1‑171
                                                                                                                                                                                GAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG                                                                                                 <  1:232342/171‑1
                                                                                                                                                                                       CGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCATTCGATGGTGGACTGTACCACCTACGATTTGCTGCTGGACCTGTTGGAAACC                                 >  1:302492/1‑228
                                                                                                                                                                                        ggccctTTTTTTTGAAGACGACCCCGGCCTGCACTTCCTTCTTTTTCTTTGAAGATGCGGAAGATCCCGCCGGTAACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCA                                <  2:554946/222‑1
                                                                                                                                                                                        GGCCCGTTTCTTTGAAGACGGCGACGGCCTGCATCTCCTTCTGCTTCTTTGAAGATTCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGGGAACTGCCCGCTTTGCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCA                                <  1:364696/228‑1
                                                                                                                                                                                                           GACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACGGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTCCGAAACCAAAATCGAACAGGTGGCAGA             >  2:632320/1‑228
                                                                                                                                                                                                                     ttccTCTCCTTTTTCTTTTTTGAAGATCCGGATGTTCCCGCCTGTACCCCCCCTGGGGCATTTACGCTCCGTGATGGTCGTCGTGTTACTCTGCGTGAGCGGGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCCATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAA   <  2:46095/224‑1
                                                                                                                                                                                                                     TGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTC                                                                                                                                                        >  1:292457/1‑79
                                                                                                                                                                                                                     TGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAA   <  1:123760/228‑1
                                                                                                                                                                                                                      tccTCTCCTTTTTCTTTTTTTAAAGTGGAGGAGATCCGGCCGTTAACTCCAGTGTGGCATTTACCATCCTTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAA   <  2:167479/224‑1
                                                                                                                                                                                                                      GCATCTCCCTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCG                                                                                                                                                       <  2:292457/79‑1
                                                                                                                                                                                                                      GCATCCCCTTCTTCTTCTTTGAAGATGGGGAAGTTCCCTCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAA  <  2:565591/228‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                          
TGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001427‑4001644
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAA  >  NC_000913/4001650‑4001873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAG  >  NC_000913/4001415‑4001883
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
GAGGCCCGGTCAAGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAG                                                                                                                                                                                                                        <  M01186:129:000000000‑A5M8V:1:1103:17176:5695/250‑1 (MQ=60)
       GGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCCTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATTCGGAA                                                                                                                                                                                                                >  M01186:129:000000000‑A5M8V:1:2107:7728:20896/1‑251 (MQ=60)
         TCATGATGAGCGCATTGCAACGGGAAAATAACCGAATGGCCCGGCTGGAAGTCGAAGAGTCAGACCCCCTTGGAAAGGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAG                                                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:1112:22543:9823/250‑1 (MQ=60)
                        TTCAATGGGAAAATAACCGACTGACACGGCTGGAAGTCGAAGAGTCAAAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTC                                                                                                                                                                                               <  M01186:129:000000000‑A5M8V:1:2108:8536:13021/251‑1 (MQ=60)
                         TCAACTGGAAAATAACCGGCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCC                                                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1103:19775:18048/251‑1 (MQ=60)
                         TCAACTGGAAAATAACCGATCTCCCCGGCTGGAAGTCGAAGAGTAAACACCCCTTGAAAAGGCAGTAGGGATTGATCTTGGCGAACCGGGCGACGACGAGCTACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCC                                                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:2106:23499:22629/251‑1 (MQ=60)
                              TGGAAAATAACCGTCTGACCCGGCTGGAAGGCGAAGAGGCCCAACCCCTTGGAAAGTCAGTATGGATTGATTTTGGCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                                                                                                                         <  M01186:129:000000000‑A5M8V:1:1104:12121:25191/251‑1 (MQ=60)
                               GAAAAATAACCGGCTGGCCCGGATTGAAGTCGAAGAGTCACAACCCCGTGTAAAGGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTGGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                                                                                                                         <  M01186:129:000000000‑A5M8V:1:1111:27495:21411/250‑1 (MQ=60)
                                 aaagtgtgcggtcgacgcggttggaagtcaaagagccacagctccgtgaaaagGCAGTAGGGATTGGTGTTGTCGAACCGGACGCCGTCGAGCGCGTGCGCGTACAATCGGAACGTGGCAGGCGCCTGGCAACCCGACCGGAACTGGAAGACAGCGAAGCATCGGCAGGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGC                                                                                                                                                                                      <  M01186:129:000000000‑A5M8V:1:1108:19536:5958/198‑1 (MQ=60)
                                    ATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTTAAGATTCGGAAGATCACGCCGGTAACTCCAC                                                                                                                                                                                            >  M01186:129:000000000‑A5M8V:1:1103:22655:15641/1‑242 (MQ=60)
                                       ccaggtgtcacttggactcggagatgggtaaaagaggcagTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGCAGACGGAGAGCGACTGCGCGTACAATCTGACCTTGGCCAGGGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAC                                                                                                                                                                                <  M01186:129:000000000‑A5M8V:1:1101:12823:25600/211‑1 (MQ=60)
                                         CGACTGACCCGGCTGGAAGTCGGAGAGTCACAACCGCTGGTAAATGGAGTATGGATGGGTCTTGTCGAACCGGACGCCGACGAGCGACTGGGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGTAAGATCACGCCGGTAACTCCACTGTGGCATTTACCA                                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1109:25996:7930/251‑1 (MQ=60)
                                                                   GTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGCCGTCTGTTTCCT                                                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1110:9430:10607/1‑248 (MQ=60)
                                                                         ACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGTTCGGCTGTTTACTCTGCGTGAG                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:2101:22196:12230/1‑251 (MQ=60)
                                                                          tggtaaaaagcaaaggcagggtttgcttTTGTCGAACCGGACGGCGACGAGCGACTGCGCGTACAAACTGAACTTGGCCAGGGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAG                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:2110:9333:20754/222‑1 (MQ=60)
                                                                           CCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCCCGTTTCTTTGAAGACGACGCCGGCCTGCAT‑‑‑‑‑CTCCTACTTCTTCTTTGAAGATACGGAAGATCACGCCGGGACCTCCACTTTGGCCTTTTCCATCCtttatgtaccgctgttgactctgcggtagcg                                                                                                                                            >  M01186:129:000000000‑A5M8V:1:1112:16710:16770/1‑220 (MQ=60)
                                                                               TGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCCATGTGGCATTTACCATCCGTGATGGTCGTCTGTTTctggctcttatccacat                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1104:17205:22210/1‑233 (MQ=60)
                                                                               TGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTCCGTGGCCGTGA                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1106:10396:7382/1‑250 (MQ=60)
                                                                               TGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGTATGGTCGTCTGTTTctgtcccttatacccat                                                                                                                                         >  M01186:129:000000000‑A5M8V:1:1102:24381:22671/1‑233 (MQ=60)
                                                                                         GTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGCCGTCTGTTTACTCTGCCTGCGCGGTAACTGCCCGCTT                                                                                                                              >  M01186:129:000000000‑A5M8V:1:2101:16521:26095/1‑251 (MQ=60)
                                                                                                  GATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGACACGCTTCTTTGAAGACGACGACGGCCAGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCTTCTGTTTACTCTGCtggaggcgtgacaccccgcttttcttct                                                                                                                       >  M01186:129:000000000‑A5M8V:1:1106:10688:3439/1‑221 (MQ=60)
                                                                                                      TTGGCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCG                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2105:17301:24526/251‑1 (MQ=60)
                                                                                                          cgaaccggagcagcgcgaggggctgagcgttcaatctgaccgtggcaatAGCCTGGCAACCCGTCCGGAACTGGAAGATAACGATGCATGGGCACGTTTCGTTGAAGACGACGGCGGCCTGCA‑‑‑‑‑GCGCCGTCTTGTTATGTGAAGATGCGGAAGTGCACGCCGGTAAGTCGACTGAGGTATTTACTATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT                                                                                                              <  M01186:129:000000000‑A5M8V:1:1102:9113:26249/201‑1 (MQ=60)
                                                                                                            acccggaccgcgacgggcggcggcggGTACAATCTGAACGGGGCCAGGGCCGGGCAACCCGCCCGGAACTGGAAGCCATCGAAGCATCGGCACGTTTCTTGACAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTTTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCG                                                                                                           <  M01186:129:000000000‑A5M8V:1:1112:26256:20166/225‑1 (MQ=60)
                                                                                                                    GACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGT                                                                                                                                                                   >  M01186:129:000000000‑A5M8V:1:1110:8446:21570/1‑187 (MQ=60)
                                                                                                                          GAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTTGTTTGTATCGTAgcgggcgccgtagccaga                                                                                             >  M01186:129:000000000‑A5M8V:1:2108:22911:3711/1‑233 (MQ=60)
                                                                                                                                     ccgaccatttacactgtgccatatccgggccaccggcccggaattggaatgcgtcgaatcgtcgggACGTTTCTGTGAAGGCGACGCCGGCCTGCAT‑‑‑‑‑CCCCTTCTTCTTCTTTGAAGATGCTGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGAC                                                                                  <  M01186:129:000000000‑A5M8V:1:1106:6091:7966/185‑1 (MQ=60)
                                                                                                                                     CGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAG                                                                                                                                                                                                                        >  M01186:129:000000000‑A5M8V:1:2105:22948:26477/1‑117 (MQ=60)
                                                                                                                                        aaaatctgacctgggccggacgCTGGCAACCCCCCCCGAACTGGCAGGCATCTAAGCATCGGCACGTTTCTTTGAAGACGACGCTGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTCCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTATGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGT                                                                               <  M01186:129:000000000‑A5M8V:1:1114:7288:15364/229‑1 (MQ=60)
                                                                                                                                         gggtctcgtgggctcggagatttgtataagaaacaGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGGCGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGT                                                                               <  M01186:129:000000000‑A5M8V:1:2107:23565:7656/215‑1 (MQ=60)
                                                                                                                                                     GGCAAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAATCTGCGTGAGCGTGACTTGCCCGCTTTTCGCTTGAATCGTATGCGTGCCCGTCGCCAGTCGATGGTAGACGGTAAAGCCTACGAGT                                                                  >  M01186:129:000000000‑A5M8V:1:2103:23575:20665/1‑251 (MQ=60)
                                                                                                                                                      gccatggccgggcagCCCGCCCGGACCTGGAGGCCATGGAAGAATCGGCACTTTTCTTTGGAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGT                                                                  <  M01186:129:000000000‑A5M8V:1:1101:21764:23290/235‑1 (MQ=60)
                                                                                                                                                            GCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTA                                                                                                               <  M01186:129:000000000‑A5M8V:1:1107:9310:24220/199‑1 (MQ=60)
                                                                                                                                                                                CTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGCCGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCC                                                                                                <  M01186:129:000000000‑A5M8V:1:1108:10571:15224/194‑1 (MQ=60)
                                                                                                                                                                                        CATCGAAGCTTCGGCCCGTTTCTTTGAAGACGGCGACGGCCTGCAT‑‑‑‑‑CTCCTTCTGCTTCTTTGAAGATTCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGGGAACTGCCCGCTTTGCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAG                               <  M01186:129:000000000‑A5M8V:1:2101:15629:19971/251‑1 (MQ=60)
                                                                                                                                                                                        CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCATTCGATGGTGGACTGTACCACCTACGATTTGCTGCTGGACCTGTTGGAAACCAAAATCGCACAG                               >  M01186:129:000000000‑A5M8V:1:1111:10510:18970/1‑251 (MQ=60)
                                                                                                                                                                                                                     aagcCGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAG  <  M01186:129:000000000‑A5M8V:1:1104:18306:20126/247‑1 (MQ=60)
                                                                                                                                                                                                                      CGACGACGGCCTGCAT‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTCGTCTGTTTACTC                                                                                                                                                      >  M01186:129:000000000‑A5M8V:1:1111:10053:7786/1‑102 (MQ=60)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
GAGTCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAG  >  NC_000913/4001415‑4001883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑