Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 89 94.4 2603848 74.4% 1937262 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,131 G→A 20.4% A337T (GCC→ACC)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,1310GA20.4% 131.1 / 34.1 73A337T (GCC→ACC) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base G (27/31);  new base A (8/7);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

TCGACGGTGGCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAA  >  NC_000913/1195895‑1196364
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
tCGCCGGTGGCCCGTTGCTGAAAGTTAAAACCCCGAACGCGTGCAAAGAGATCGTCATTAAAGACGGGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGACCCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCGGTTTGAAGcc                                                                                                                                                                                                                                          <  2:733414/239‑1 (MQ=255)
    cGGTGGCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAg                                                                                                                                                                                                                                            >  1:1091021/1‑233 (MQ=255)
     ggTGGCCCTTGGTTGAAAGTTAAAAACCCGAAACCTGGAAAAGAGTACGTCATTAAAGGCGTGAGTGCGGATGCATGCCTGCAACAGTTCCTGCTGCGTCCGGCTGAATATGATGTTATTGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGGTTGAAAccaccca                                                                                                                                                                                                                                     <  2:991934/239‑1 (MQ=255)
      gTGGCCCGGGTTTTAAAATTAAGAACCCGTGCACTGGCACAGAGGTCGGTCTTAAAGACGTTATTGCTGAGTCCTTCCCGCAACAAATCCCTCTGCGTCCGGCTGAATGTTATGGTACCGCATGTAGGAACCTGAACCGTGACTACATTT‑CTGGCGCGCTGGCAGCCCAGGTTGGCGGTATCGGTA‑TCGCCCTTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGcctccca                                                                                                                                                                                                                                     <  2:7337/238‑1 (MQ=255)
         gCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCCGGTTGGCGGTATCGGGA‑TCGCCCCTGGTGCAACCATCGGTGACGAATGCGCCCTGTTTGAGGCCACCCACgt                                                                                                                                                                                                                                  >  1:793090/1‑237 (MQ=255)
         gCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGGATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGt                                                                                                                                                                                                                                 >  1:374450/1‑239 (MQ=255)
          cccggtgatGAAAGTTAAAACCCCGAACCCTGGCAAAGAGATCGTCATGTAGGAGGTTATGTCGGATGCATGCCTGGATACGGTCCTGCTGCGGCCGGCTGAATATGATGTTATCGCCTGTATGGACCTGAACGGTGACTACATTT‑CTTACGGCCTGGCAGCGCAGGTTGGCGGTAGCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTa                                                                                                                                                                                                                                <  2:389229/231‑1 (MQ=255)
           ccGTGGCTGAAAGTTAAAAACCCGAACCCTGGCAAAGGGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTa                                                                                                                                                                                                                                <  1:1298479/238‑1 (MQ=255)
              tGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGAAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGGACTg                                                                                                                                                                                                                             >  1:87230/1‑238 (MQ=255)
              tGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGCGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTg                                                                                                                                                                                                                             >  1:347148/1‑238 (MQ=255)
                gCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTgcg                                                                                                                                                                                                                           <  1:1281387/238‑1 (MQ=255)
                     aatttAAAAACCCGAACACTGGCAAAGGACTCGTCATTAAATGCGTTATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTTGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGaa                                                                                                                                                                                                                      <  1:646220/235‑1 (MQ=255)
                         ttaaacCCCGAAACCTGGGAAAGGGATCGTTCTTAAAAGCGTTATTGCGGATGCGTTCCTGCAACAGATCCTGGTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATg                                                                                                                                                                                                                 <  2:385789/237‑1 (MQ=255)
                              aCCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGt                                                                                                                                                                                                            <  1:263141/239‑1 (MQ=255)
                                  gAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAgg                                                                                                                                                                                                        >  1:704470/1‑239 (MQ=255)
                                    acccTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGAc                                                                                                                                                                                                      <  1:42345/236‑1 (MQ=255)
                                              agagATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGGGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGCCGGTATCGGGA‑TCGCCCCTCGTGCAAACATCTGTGACTAGAGCGCCCGGTTTGTAACCCCCCCCGGGACCGAGCCCAAAAAAGCCAGACAGGCAAAAGaaaaac                                                                                                                                                                                            >  2:111905/1‑237 (MQ=255)
                                                agaTCGGCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCt                                                                                                                                                                                          <  1:939565/239‑1 (MQ=255)
                                                 gtttcttaTTAAAGACGTGATTTGTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCt                                                                                                                                                                                          <  2:1077554/231‑1 (MQ=255)
                                                 gaTCGTCATTAAAGTCTTGATTGCTGGTGCATTCCGGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACTGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATcc                                                                                                                                                                                           <  2:34260/237‑1 (MQ=255)
                                                   tgtgcATTAAAGGTGTGGTTTCGGATGCTTTCCCGAAAACAATCCGGCTGCGCTCGGCTGAATATGATGTTATCCCCTGTATGAAACTGAGCGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGGTACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTgg                                                                                                                                                                                        <  2:659780/234‑1 (MQ=255)
                                                   tCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTgg                                                                                                                                                                                        <  1:1272238/238‑1 (MQ=255)
                                                    cGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGGCAAAGTAAATCCTGGc                                                                                                                                                                                       >  1:34260/1‑238 (MQ=255)
                                                    cGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TAGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTCTGAAGCACCCCCCGGTACTTGCCCGAAATAAGCCGGTCCGGCAAAAGaaaaaacgggag                                                                                                                                                                                      >  2:841921/1‑231 (MQ=255)
                                                                 gTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGCCGAATGCGCCCTGTTTGCAGCCACCCACGGTATTGCGCCGAAATATGCCGGTCAGGCCAAAGTAAATCCCGGCTCTATTATtctcc                                                                                                                                                                          >  2:771150/1‑237 (MQ=255)
                                                                 gTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATtctct                                                                                                                                                                          >  1:348225/1‑238 (MQ=255)
                                                                     ttGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGc                                                                                                                                                                      <  1:936317/238‑1 (MQ=255)
                                                                                 tCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGAtgctg                                                                                                                                                         <  1:716686/239‑1 (MQ=255)
                                                                                       aaCAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCcac                                                                                                                                                   <  1:887099/239‑1 (MQ=255)
                                                                                            aTCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGAGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTgcg                                                                                                                                                                                                                           >  2:905832/1‑162 (MQ=255)
                                                                                            aTCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGAGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTgcg                                                                                                                                                                                                                           <  1:905832/162‑1 (MQ=255)
                                                                                              ccTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGCAATATGCCGGTCCGGCCAAAGTAAATCCTGGCCCTTTTATTTTCCCCGCTAAGACGATTCTCGCCCACACGGGtg                                                                                                                                            >  2:477447/1‑238 (MQ=255)
                                                                                              ccTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGGGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGtt                                                                                                                                            >  1:65404/1‑239 (MQ=255)
                                                                                                       tCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTTACTACATTT‑CTGACGACCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGCATGCGACCTGTTTTGAGCCACCCTCGGTACTGCGCCGCAATATACCGGTCAGGACAAAGAACACTCTGCCCCTACTATTTTATCTCCGCCGATGATTCGGCGCAACCTGGGGTGGTGCGAAg                                                                                                                                   >  2:866389/1‑239 (MQ=255)
                                                                                                           ctcaaaTATGTTGTTATGCCCTGGATGAACGTTGAGGGTGTCTACATTT‑GTGACGCCTTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACAGCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGc                                                                                                                               <  2:889942/235‑1 (MQ=255)
                                                                                                                      tgTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGcc                                                                                                                                                                                                                                          >  1:264575/1‑121 (MQ=255)
                                                                                                                      tgTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGCCTTAATTg                                                                                                                    >  1:905931/1‑239 (MQ=255)
                                                                                                                      tgTTATCGCCTGTATGAACCTGAACGGGGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGcc                                                                                                                                                                                                                                          <  2:264575/121‑1 (MQ=255)
                                                                                                                         tttCGCCTGTATGGACCGTAAAGGGGAGTTCAGTT‑CTGACGGCCGGGCAGCGTAGGTTGGAGGTAGCGGGA‑TCGCCCCGTGGGAAAAGCGCGGTGACGAATGCGCCCTGTTTGAAGCCACGTGCGGTACTGCGCCGAAATAGGCCGGGCAGGAGAAAGTAATTCCTGGCGCTATTATTCTCTCCGCTGAGATGATGATGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGtt                                                                                                                  <  2:501560/236‑1 (MQ=255)
                                                                                                                         tATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTgg                                                                                                                                                                                        >  1:656366/1‑168 (MQ=255)
                                                                                                                         tATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa                                                                                                                 >  1:269056/1‑239 (MQ=255)
                                                                                                                         tATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa                                                                                                                 <  1:1097141/239‑1 (MQ=255)
                                                                                                                         tATCGCCTGTATGAACCTGAACGGGGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTgg                                                                                                                                                                                        <  2:656366/168‑1 (MQ=255)
                                                                                                                             ccttGTTTGACCCTGAA‑GGTGTCTCCATTTAATGA‑GCCCGGGGAGCGCGGGTTGGGGGT‑TCCGTACCCGCCACTGGTGCGAACCATGGTGGCGAATGTGACCTGTTTGAAGCAACCCACGGTACTGCGCGGAAATATGCCGGTCAGGACAAAGTAAATCCTGGATCTATTTTTCTCTCTGCTGAGATGATGCTGCGCGACATGGGTTTGACCGAAGCGGCTGCCTTAATTGTTAAAggg                                                                                                            <  2:1295696/236‑2 (MQ=255)
                                                                                                                               cTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGt                                                                                                            <  1:477447/238‑1 (MQ=255)
                                                                                                                                tGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGGTGGACCGAAGCGGCTGACTTAAtt                                                                                                                     >  1:269594/1‑228 (MQ=255)
                                                                                                                                  ttttaaCCTGAATGGTTACTAAGTTT‑GCGACGCCCTGGCAGGGCAGGTGGGCGGGCTCGGTA‑TCGCCCCTGGTGAAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTTCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCGGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg                                                                                                        <  2:704470/235‑1 (MQ=255)
                                                                                                                                  tATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAACGGCTGACTTAATTGTTAAAGGTATgg                                                                                                        >  1:493024/1‑239 (MQ=255)
                                                                                                                                  tATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg                                                                                                        >  2:746433/1‑239 (MQ=255)
                                                                                                                                       aCCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCCCTATTATTCTCTCCGCTGAGATGATGCTGCCACGCATGGGTTGGCACGAAGCGGCCGACTTCATTGTTAAAGggaaggaaaga                                                                                                   >  2:521419/1‑236 (MQ=255)
                                                                                                                                         cTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGATGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGCCTTAATTGTTAAAGGTATGGAAGgcgc                                                                                                 >  1:1036142/1‑239 (MQ=255)
                                                                                                                                                          tt‑CTGACGCCCTGGCAGCGGAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCGCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCg                                                                               <  1:591183/239‑1 (MQ=255)
                                                                                                                                                          tt‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCg                                                                               <  1:111905/239‑1 (MQ=255)
                                                                                                                                                                aCGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTaa                                                                            >  1:376571/1‑237 (MQ=255)
                                                                                                                                                                       ggCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCAACATGGGTTGGACCGAAACGGCTGACTTAATTGTTTAAGGTATGGAAGGCGCA‑ATCAAAGCGAAAACCGGAACCTACGaat                                                                   >  2:211421/1‑237 (MQ=255)
                                                                                                                                                                             gcAGTTTGGCGTTTTCTTTT‑TCCCCCCTGTTGCAAACCTCGGGTACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGc                                                             <  2:493024/239‑1 (MQ=255)
                                                                                                                                                                              cAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGCATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATAACTTAGAGCg                                                            >  1:920146/1‑239 (MQ=255)
                                                                                                                                                                                         tatcggta‑tcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTTTTAACGGGATGGAAGG‑GCACAACAACGCGAAAAACGGAACATATAACTTCGAGAGTcgggaggggg                                                 >  2:435922/1‑230 (MQ=255)
                                                                                                                                                                                           tcggta‑tcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGGTGgc                                               >  1:236692/1‑239 (MQ=255)
                                                                                                                                                                                           tcggta‑tcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATGGTTAAAGGCATGGAAGGGCCA‑ATCAACGCGAAAACCGTAACCTATGACTTCTAGCGTCTGatggatggg                                               >  1:976985/1‑238 (MQ=255)
                                                                                                                                                                                                    gCCCCTGGTTCAAATATCGGTGACGAAGGGGCCATGTTTGAAGCC‑CCCACGGGTCGGCGCCGAAATAGGCCGGTCAGGAAAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActg                                      <  2:750988/239‑1 (MQ=37)
                                                                                                                                                                                                          ggggCAAACCTCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                 <  1:771150/236‑1 (MQ=255)
                                                                                                                                                                                                          ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                 <  1:872444/239‑1 (MQ=255)
                                                                                                                                                                                                              cAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGCAAATCCTGGCTCTATTATTCTCCCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTATTTGTTAAAGGGATGGAAAGCGAA‑ATCAACCCGGAAACCGAAACCTTTGACTCCTTGAGTCTAATAGATGGGCCAAAAACGCTGaaaatt                             >  2:601428/1‑236 (MQ=255)
                                                                                                                                                                                                               aaaCTTCGGTGCAGAATGGCCCCTGTTTGAAGCCACCAACGGTACTTGGCCGAAATAAGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCCCTCCGCTGAGAAGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAAGCTAGGACTGCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                            <  2:348225/239‑1 (MQ=25)
                                                                                                                                                                                                                aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCCCTATTATTCTCTCCGCTGAGATGAGGCTGCGCCACATGGGTTGGTCCGAAGAGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACCCGAGAGCAGTACCCTAGATCATCACGGGTCTCATGGGTGGCGCAAAAAGTCTGAAATGTTc                           >  2:364539/1‑239 (MQ=255)
                                                                                                                                                                                                                aaCATCGGTGACGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGGTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGGATGGAAGGCGCA‑ATCAACGCGAAAACCCTAACCCCTGACTACGGGCGTCTGTAGCATGGCGCTAAACGGTTAAAATGTTc                           >  2:62282/1‑239 (MQ=255)
                                                                                                                                                                                                                 aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTACGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                          <  1:211421/239‑1 (MQ=255)
                                                                                                                                                                                                                     cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGa                                                                                                                                     >  2:707422/1‑129 (MQ=255)
                                                                                                                                                                                                                     cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGa                                                                                                                                     <  1:707422/129‑1 (MQ=255)
                                                                                                                                                                                                                          aCGAATGCGCCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGCTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCCAAGCGAAAAACGTAACCTATGACTTCGAGCGACTGAGGGATGGGGCTAAAATGCTGAAAGGTTCAGAGTTTggg                 >  2:649206/1‑238 (MQ=255)
                                                                                                                                                                                                                               tGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTCAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGCCCGAAACGGCTGGATTAATTGTTACAGGTCTGGAAGGCGCA‑ATCAACGCAAAACCCGTAACCTATGACTTCGGGCGTCTGATGGATCTCGCTCAACTCTTGAATTGTTCCGCGTTTGGTGAcgc            >  2:63320/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gcCCTGTTTGAAACCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg           <  1:746433/237‑1 (MQ=255)
                                                                                                                                                                                                                                         ttGAAGCCCCCCCCGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCGATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa  <  1:841921/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
TCGACGGTGGCCCGTGGCTGAAAGTTAAAAACCCGAACACTGGCAAAGAGATCGTCATTAAAGACGTGATTGCTGATGCATTCCTGCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTT‑CTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTA‑TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCA‑ATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAA  >  NC_000913/1195895‑1196364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A