Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F28 I0 R1
|
51 |
55.9 |
1320120 |
86.3% |
1139263 |
218.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
3,053,526 |
T→C |
28.3% |
R439G (AGA→GGA) |
pepP ← |
proline aminopeptidase P II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,053,526 | 0 | T | C | 28.3%
| 45.1
/ 2.7
| 33 | R439G (AGA→GGA) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base T (11/10); new base C (3/7); total (14/19) |
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCAC > NC_000913/3053299‑3053751
|
ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGAATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGCCTTAACCGGGAACTAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCCCCACCGACCATGATTACGCCCATTGCTTtct > 1:95320/1‑228 (MQ=255)
tCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGCCTTAACCGGGAAATAGCAAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCCCCGCCGATGATTACGCTCATTGCTTTCCCccag‑ca > 1:314571/1‑227 (MQ=255)
tGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATCCCGCCACCGCCGATGATTACCCCCCTTGCTTTCTCGCAC‑CAccccccacccctt > 2:658106/1‑218 (MQ=255)
aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTcc > 1:520606/1‑227 (MQ=255)
aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAA‑CAACCCTCAACGCCTCGGTTACTTCCg > 1:282698/1‑228 (MQ=255)
gACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTC‑‑CGCCCGGCCCTGCCTCCACCGCCGATGGTTCAGCCCATTCCTTT‑TCGCCGCCAACCACCAACCCTTCGATTTCTTCCGTCTGTTCCCCCACACt > 2:5883/1‑228 (MQ=255)
cgccAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGCCG‑CGCCC‑GCCCTGCCCCCACCGACGATGATTCCGCCCATAGCTTTCTCTCAG‑CAACCCTCAACGCTTCCATTTATTCCCGCGTTTTCACCACGCTGACGGGGAggggc > 2:373984/1‑225 (MQ=255)
aTCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCCCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGTTTTCGTTACCg > 1:366675/1‑227 (MQ=255)
ctttctCCCTCAAAACCCGGGTGGAGCGTGGGACTGGGGCGCAGGCGCTTAAATCAAGTGGCCGTGCAGCCCCCC‑GTGACTGAACCGGAAAATGGCCACCG‑CCAGCCTCG‑ACCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGtt < 2:314571/223‑1 (MQ=255)
cGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGAAAGGGTTTAGTTAACGGTTTTgg > 2:558851/1‑228 (MQ=255)
aaaGCCGGGAG‑GAGCCTGTGACTCGGGCGCCGTCGCTTCAATCAAATGGCCCGGCCGCCCCCC‑GTGAATTAACCGGGAAATAGCAAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATcaca < 1:610398/228‑1 (MQ=255)
gAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGCCGGTGAGGTTTTCTGTTCCGGTTTTGG‑TAATCACAATtttgtc > 1:405398/1‑228 (MQ=255)
tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACAACGCTGGCGGTGAGGGTTTCTTTTCCCGTTTCTG‑GAATCACACAGTTGGCTTCCATTAGAATTCCg > 1:644964/1‑228 (MQ=255)
tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCACCAACGCCTCGATTTCTTCCGGCTTTTTCACCCCGCTGGCGGCGTGGTTTTCGTTTCCGGGTTCGGATACGCACAA‑GTCGGCTTCAAACCAAATGcca > 2:7477/1‑227 (MQ=255)
gcggAGTCTCTTCAACAAAATG‑GCCGGGAGCCCCCCGGTGCTCTAACCGGGAAA‑AGCCCGGGCCCAGCGTCG‑GCCCC‑GCCATGCCGCCACCGGCGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGa < 2:95320/227‑1 (MQ=255)
gcctgatCGCTTAAATCAATGGTCCCGGAAGCGCCCC‑GTGATTTAACGGGGAACTCCCCAGCG‑CCCGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGa < 1:364864/221‑1 (MQ=255)
tCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGTTTTCTTTTCCGTGTTCGG‑TACTACCAATATCGTCTTCATAACAAAAGCCggcaacccg > 2:572965/1‑220 (MQ=255)
aTGTCCCGGAAGCCCCCG‑GGGACTTAACCGGAAAATGCCCAGCG‑CCAGGTGCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg < 1:115327/227‑1 (MQ=255)
gcCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTCTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCCAGACCGCGATATTGTTTTTGCACTTTTTCATCCg > 1:418894/1‑228 (MQ=255)
cccc‑GTGACTTAACCGGGAAATAGCCAGCG‑CCCGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCgg < 1:414270/228‑1 (MQ=255)
cggTGACTGAACAGGGAATTACCCAGCG‑CCGGCGCCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGATCATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgcg < 1:19642/226‑1 (MQ=255)
cGGGAAATGGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGATTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc < 1:104070/228‑1 (MQ=255)
ccgccgcg‑cccgccTCC‑CGCCC‑GCCATGCCGCCACCGCCGATGATTACGCTCATTGCTTTCGCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct < 1:592296/223‑1 (MQ=255)
gcccccg‑cccgcgTCGCCCCCC‑GCCATGCCCGCACCGATGCTGTTTACCCTCAGTGCTTTCCCGCAG‑GACCCATAAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct < 2:282698/223‑1 (MQ=255)
cgccc‑gccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTAAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc < 1:558851/228‑1 (MQ=255)
gccc‑gtcATGCCGCCACCGACGGTGATTACGCCCATTGCTTTCGCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc < 1:60520/227‑1 (MQ=255)
cccc‑ggcATGCCGCCACCGACGATGATTACGTTTATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg < 1:339067/224‑1 (MQ=255)
gccgttcatTACGCTAATTTCTTTCCCGCGA‑CCACCATCATCGCTTGGATTTCTTCCGGCTTATTCCCCGCGCTGGCTGGGAGGTTTTTGTTGCCGGTGTCGG‑AAATCACAATGTCGTCGTCGATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCGCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag < 2:578370/221‑1 (MQ=255)
ttACGCCCATTGCTTGTCCGGCG‑CAACCATTAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTg < 1:5883/228‑1 (MQ=255)
tccGCTCCTTGCTGTCCCTGAG‑CAGCCATAACCTCTTGGTTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCCGAATTTCGTCTTCAATACTAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCGAGAATGCGCGAGCGATCCTGa < 2:478561/226‑1 (MQ=255)
tacggccATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTTCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGa < 1:658106/222‑1 (MQ=255)
cTCATTGGTTTCCCGCAG‑CAACCATCCACGCTGCGATTTCGTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAt < 1:222791/228‑1 (MQ=255)
cTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑Taa > 2:525120/1‑93 (MQ=255)
tCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAt < 1:525120/93‑1 (MQ=255)
ctcGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCAATTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCCAGCCATCCTGACCATAAACACCCAc > 1:204590/1‑227 (MQ=255)
|
TTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCAC > NC_000913/3053299‑3053751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
