Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 51 55.9 1320120 86.3% 1139263 218.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAA  >  NC_000913/3525724‑3526215
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
TTGCTCATCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAATTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGA                                                                                                                                                                                                                                                   <  M01186:129:000000000‑A5M8V:1:1102:19511:13329/251‑1 (MQ=60)
TGGCATATCGCCTTCCAGTGGGTCCGAGTTGAGATCGTGCGCTGCCAGGATAGTCAGGTTGCTGGAAAAGTAAGGCGGTGCAATGCTGAGCTTCTTCAAAAAAGTGAGATCGTTCGCTCCGAAGCCAACCTGTTCTATTAGCTCGTGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATGCGTAGGATTCAGGTCGCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGA                                                                                                                                                                                                                                                   <  M01186:129:000000000‑A5M8V:1:1113:27192:7829/251‑1 (MQ=60)
 tgtttATCGCCTTCCAGTGAGTCCGGGTAGAGATCTTGCGCTACCACGATATTTATTTTGCTGGAAAAGTAAGGCGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCGCGTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGat                                                                                                                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:1102:16700:28484/246‑3 (MQ=60)
 GGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCGGTTCCCACTGCGAATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGga                                                                                                                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:1113:17321:21226/1‑249 (MQ=60)
  ggtcagcggCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGat                                                                                                                                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:2108:16251:20528/241‑3 (MQ=60)
     TATCGCCTTGCAGGGATTCCGGGTAGAGAGCTTGCACTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGatcagg                                                                                                                                                                                                                                              <  M01186:129:000000000‑A5M8V:1:1107:23146:3939/251‑7 (MQ=60)
       TCGCCTTACAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGatcaggtg                                                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:1113:25476:17900/251‑9 (MQ=60)
             TCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTC                                                                                                                                                                                                                                            <  M01186:129:000000000‑A5M8V:1:2112:27227:9026/251‑1 (MQ=60)
                                 ATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGatcaggt                                                                                                                                                                                                                                             <  M01186:129:000000000‑A5M8V:1:2105:9329:16763/224‑8 (MQ=60)
                                     ttggttccacCGATATTCACTTTGCTGGAAAAGTAAGGCGGTGCCCTTCTTCGGTTCTTCATAAAACGCAGATCGGTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGACTTCGTAGACGCTTTCCCCCGGATCAATTAATCCTTTCGAAAACCCTAATTCGTAGGCTTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGC                                                                                                                                                                                                                    <  M01186:129:000000000‑A5M8V:1:2102:12041:17168/241‑1 (MQ=60)
                                      GCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCA                                                                                                                                                                                                                           >  M01186:129:000000000‑A5M8V:1:2110:28333:13902/1‑243 (MQ=60)
                                           ACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCG                                                                                                                                                                                                              >  M01186:129:000000000‑A5M8V:1:1108:27123:8680/1‑251 (MQ=60)
                                                                 AAAAGTAAGACGGTGCCATGCTGGGCCTCTTCAAAAAAGGCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTAACCCGTATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAAC                                                                                                                                                                                   <  M01186:129:000000000‑A5M8V:1:2107:14701:27000/250‑1 (MQ=60)
                                                                        AGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAcaa                                                                                                                                                                                  >  M01186:129:000000000‑A5M8V:1:1104:10569:15802/1‑247 (MQ=60)
                                                                          accggtCCATGCTGAACTTCTGCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTCGCTCGCGGTTAGCGGCTTCGGAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACGC                                                                                                                                                                                 <  M01186:129:000000000‑A5M8V:1:2106:9910:5196/243‑1 (MQ=60)
                                                                                         tcggggatcctctctggtctcgggggctcggggagggttataagagacagAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTG                                                                                                                                                                <  M01186:129:000000000‑A5M8V:1:2101:21615:2659/201‑1 (MQ=60)
                                                                                         TCTTCCTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTG                                                                                                                                                                <  M01186:129:000000000‑A5M8V:1:2108:14405:19013/251‑1 (MQ=60)
                                                                                               TCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGTCACAATCTTCACTGCTTCCCGGTTGGTTGGACcacttcgttcctaaaaaccccccagcccatgcctgaactc                                                                                                                                                           >  M01186:129:000000000‑A5M8V:1:2114:8738:20263/1‑210 (MQ=60)
                                                                                                    AAAGTCAGGTCCTTCGTCCCAAATCCAACCTTTTCTATTAACTCGAGGTTAGCGGCTTCGTCGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTGCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATC                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:2112:20731:25057/251‑1 (MQ=60)
                                                                                                    AAAGTCAGATCGTTCGCTGCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGAACAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATC                                                                                                                                                     <  M01186:129:000000000‑A5M8V:1:1114:26370:14739/251‑1 (MQ=60)
                                                                                                         CAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCAATGCTTCCCGGTTGGTTGGACGCATTCGGTCATCAACACGCCGCACGCCATTGCTGAACTCCAGATCCACG                                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:2103:11727:18680/1‑250 (MQ=60)
                                                                                                                       CAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCCGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAAC                                                                                                                                  <  M01186:129:000000000‑A5M8V:1:1101:15602:8098/251‑1 (MQ=60)
                                                                                                                         AATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACCCTCTCCACGGTAAACA                                                                                                                                 >  M01186:129:000000000‑A5M8V:1:2105:19512:5077/1‑250 (MQ=60)
                                                                                                                                 CGCTTATTTTAGCTCGCTGTTAGCGGCTTCGTAGACCCTTTCCCCCTGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTCCCCACTGCGTTCTCGCGGGTCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCAGCACTGCTTCCCGGTTGGTTGGGCGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAAC                                                                                                                        <  M01186:129:000000000‑A5M8V:1:2106:10570:3721/251‑1 (MQ=60)
                                                                                                                                    TTCTTTTAGCTCCGGGTTAGCGTCTTCGGAGGCGCTTTCCACCGGATCAATTAGTCCTTTCGAAAACCCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAAGCGGCACAATCATCCCTGCTTCCCGGTTGGTTGGACGGATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAG                                                                                                               <  M01186:129:000000000‑A5M8V:1:1108:6701:23782/251‑1 (MQ=60)
                                                                                                                                                                    CGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAAC                                                                                                                        <  M01186:129:000000000‑A5M8V:1:2107:19724:19999/210‑1 (MQ=60)
                                                                                                                                                                      CTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGAGTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTG                                                                                   <  M01186:129:000000000‑A5M8V:1:2109:20237:6949/251‑1 (MQ=60)
                                                                                                                                                                             CCGGATCAATTAATCCTTTCGAAAACCCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTC                                                                      <  M01186:129:000000000‑A5M8V:1:2108:23011:12665/251‑1 (MQ=60)
                                                                                                                                                                              CGGATCAATTACTCCTTTCGAAAACCCTAATTCGTAGGATTCAGTTCCCCCTGCGTATCCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCGCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATT                                                                           <  M01186:129:000000000‑A5M8V:1:1112:11442:9099/251‑1 (MQ=60)
                                                                                                                                                                              CGGATCAATTAATCCTTTCGAAAACCCTAATCCGTAGGATTCAGTTCCCATCGCTTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCCCTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATT                                                                           <  M01186:129:000000000‑A5M8V:1:2108:12680:15187/251‑1 (MQ=60)
                                                                                                                                                                                             TTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGGATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCAAAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:2110:20527:21759/251‑1 (MQ=60)
                                                                                                                                                                                                   AAAACCTAATTGGGAGGATTCAGTTCCCAGTGGGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATGGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:2102:20502:6035/251‑1 (MQ=60)
                                                                                                                                                                                                   AAAACCTAATTCGTAGGATTCAGTTCCCATGGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGGCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTTGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:1107:14658:4098/251‑1 (MQ=60)
                                                                                                                                                                                                   AAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:1102:8150:15036/251‑1 (MQ=60)
                                                                                                                                                                                                   AAAACCTAAGTCGTAGGGTTCAGTTCCAGCTCCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑TCAGGAACAGGTGATCGTCCCCAACCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACTCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:2109:9811:17924/251‑1 (MQ=60)
                                                                                                                                                                                                   AAAACCTAAGTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGC                                                      <  M01186:129:000000000‑A5M8V:1:2106:20430:15398/251‑1 (MQ=60)
                                                                                                                                                                                                      ACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGCGGATCAGGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAAT                                                                                               >  M01186:129:000000000‑A5M8V:1:2103:7238:11091/1‑207 (MQ=60)
                                                                                                                                                                                                          AATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCG                                                                                                                             <  M01186:129:000000000‑A5M8V:1:1109:20296:15410/167‑1 (MQ=60)
                                                                                                                                                                                                          AAATCGTTGGCTTCAGTTCCCACTGCGTATTCTC‑‑‑‑‑‑GGATCAGGATCCAGA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTTATCCGCCACAATCGGCCCAATCATCCCTGCTTCCCGGTTGGTTGGACGCATTCGTTCCTAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAA                                               <  M01186:129:000000000‑A5M8V:1:2106:15183:21481/251‑1 (MQ=60)
                                                                                                                                                                                                                         ATTCCCGCTGTGTATTCGC‑‑‑‑‑‑GGCTCAG‑‑‑‑‑‑GATACGGCT‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGGATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTG                                <  M01186:129:000000000‑A5M8V:1:2104:15607:22010/251‑1 (MQ=60)
                                                                                                                                                                                                                                               tcaggaaCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGC          <  M01186:129:000000000‑A5M8V:1:1106:11990:17460/244‑1 (MQ=60)
                                                                                                                                                                                                                                                       AG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAA  >  M01186:129:000000000‑A5M8V:1:1102:21735:14352/1‑251 (MQ=60)
                                                                                                                                                                                                                                                                A‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGAAACGCTTTCGGC          >  M01186:129:000000000‑A5M8V:1:2102:12725:5524/1‑240 (MQ=60)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
TGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCTTTTAGCTCGCGGTTAGCGGCTTCGTAGACGCTTTCACCCGGATCAATTAATCCTTTCGAAAAACCTAATTCGTAGGATTCAGTTCCCACTGCGTATTCGC‑‑‑‑‑‑GGATCAG‑‑‑‑‑‑GA‑‑‑‑‑‑T‑‑‑‑‑‑CAGGTGATCGTCCACAATCGGCACAATCATCACTGCTTCCCGGTTGGTTGGACGCATTCGTTCATAAACACGCCGCACGCCATTGCTGAACTCCAGATCCACGCTCTCGACGGTAAACAGTCGGGAACGGGCTACAGTTTCAACATTCAGAATGGTGGGTTTTTGTAATGATTTGCTCATCGTGGGATCTATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAA  >  NC_000913/3525724‑3526215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑