Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	98	90.7	2214160	82.3%	1822253	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,120,818	A→G	21.1%	L246P (CTC→CCC)	wcaJ ←	colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,818	0	A	G	21.1%	109.3 / 6.0	53	L246P (CTC→CCC)	wcaJ	colanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand): ref base A (10/31); new base G (10/2); total (20/33)
Fisher's exact test for biased strand distribution p-value = 3.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120590‑2121053

aTCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGGAAGCGGCGTGTTGTAAAGCGGCACCACCGGGACGCCGTTCCTCTCTTCGA‑GGGGTGGATGGGGAATGTTgga                                                                                                                                                                                                                                   >  1:934668/1‑237 (MQ=255)

    gCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGACCCCCCGGTACGCCGTTCATTTCTTCCA‑GGCGGGGATGGGGAATGTTGAAGGTa                                                                                                                                                                                                                               >  1:947276/1‑239 (MQ=255)

      ttGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGGGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGGGAATGGGGAATGTTGAAAGGaaa                                                                                                                                                                                                                             >  1:275251/1‑239 (MQ=255)

        gCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGTCAGCACAATGTCTTCCGGACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGGGGAGCGTAAAGCGGCACCCCCGGTACGGCGCTCACCTCCTCGAGGGCGCGAA‑GGAGAAGGCTGCAGGGaaaaa                                                                                                                                                                                                                           >  2:661488/1‑237 (MQ=255)

               atGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGTAAAGCGGCACCACCGGTACGCCGGTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGGAAAAACGTCggg                                                                                                                                                                                                                    >  1:247344/1‑239 (MQ=255)

                tGCCGTAGCGGGTCGGGCGGAAAATACCCGGCCTTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                   <  1:1083219/239‑1 (MQ=255)

                ggcggtggCGAGTCTGGCGGAAAATACCCGGCCCGGGTAAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCgggg                                                                                                                                                                                                                   <  1:863602/232‑1 (MQ=255)

                   cGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAACGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCCCTTCCA‑GGGGGGAGTGGGGAATGTTTAAGGGAAAGACCTCGGGGGTc                                                                                                                                                                                                                >  1:407794/1‑239 (MQ=255)

                     tAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAAACCCGGAAAGCGGCGTGTCGGAAAACGGCCCCACCGGTACGCCGTTCATCTCTTCCA‑GGCGTGAATGGGGAATTGTTAAGGGAAAGACGGCGGGGGTcag                                                                                                                                                                                                              >  1:1087918/1‑239 (MQ=255)

                     gaggcaGTCTGGGCGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATcag                                                                                                                                                                                                              <  1:399014/234‑1 (MQ=255)

                         gtgTCGGGGGGAAAATACCCCGCCCGGGGGAACTGGTTTTACCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCagcagc                                                                                                                                                                                                          <  2:247344/237‑1 (MQ=255)

                           ttcaggCGGAAATAAACCGGCCCTGGTGAATGGAGTTTCCCCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAgcagca                                                                                                                                                                                                         <  1:757851/234‑1 (MQ=255)

                            tCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGGGTCGGAAAGGTGCACCACCGGTACGCCGTTCATCTCTTCGC‑GGCGGGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGGTCaacagccac                                                                                                                                                                                                       >  2:855197/1‑236 (MQ=255)

                            tCTGGCGAAAAATACCCGGCCCTGGTGACCTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        <  1:165824/238‑1 (MQ=255)

                               ggCGAAAAATACCCGGCCCTGGTAAACTGGGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGaa                                                                                                                                                                                                    <  1:715521/239‑1 (MQ=255)

                                                    gTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGGATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGGGGGGTCCGCCCGTTGa                                                                                                                                                                               >  1:573972/1‑239 (MQ=255)

                                                        accggttttCCCCGCCAGCGCAATCCAGCAACGCCCCGGGGAGATCAGGAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGa                                                                                                                                                                           <  2:1087918/233‑1 (MQ=255)

                                                         cTGAGTTCCACCGCCAGCGCAATACAGCACAGCCCCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGAc                                                                                                                                                                          <  1:429265/239‑1 (MQ=255)

                                                                          gccAATACCGACCAACACCGGGGGGGGCCGGAGCAGATAAGGCGCCGCCAGCAAAATGGCATCCTCGCGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGGGTGCCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGGCCAGTTTTTTCACTcgcg                                                                                                                                                         <  2:395733/237‑1 (MQ=255)

                                                                                       agcacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGGAAAGGGGGGGGCCGCCCGTTGTTGGGCCAGGTTTTTCCCCCGCGCGCCGCCGCCCAt                                                                                                                                            >  1:941945/1‑239 (MQ=255)

                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgag                                                                                                                                                                                                                                            <  2:701813/141‑1 (MQ=255)

                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGgag                                                                                                                                                                                                                                            >  1:701813/1‑141 (MQ=255)

                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGGGTCCGCCCGTTGATGGACCAGGTTTTTTCCTCGCGCGCGGTGGCACAttt                                                                                                                                          >  1:588820/1‑239 (MQ=255)

                                                                                         cacCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGCGTCGTAAAGCGGCACCACCGGTAAGCCGGTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGGACAGACGTCGGGGATCAGCACAACCGAAAAGAGGGTGTCCGCCAGTGGGGGGACAAGTTTTTTCACCAGCGCGCCGGCGcacaatg                                                                                                                                          >  2:177755/1‑236 (MQ=255)

                                                                                          cccGGGGAGATCAGCCGCAAAATAAGCGGCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGGAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                         <  2:559389/238‑1 (MQ=255)

                                                                                           ccGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTg                                                                                                                                         <  1:855197/238‑1 (MQ=255)

                                                                                             gggggcgtCAGCAGCAGAAAAAGGCGCGCCAGGCCAAGTTCTTCCGCACGTTTGGGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCAt                                                                                                                                      <  2:1072072/232‑1 (MQ=255)

                                                                                               ggAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATc                                                                                                                                     <  1:292315/238‑1 (MQ=255)

                                                                                                gagaTCCGCAGCAAAATAAGCCTCCCCAGCACAATGTCTTCCGCACGTTTGAGCAGGGGGTTAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                    <  2:955612/238‑1 (MQ=255)

                                                                                                gagaTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATcg                                                                                                                                    <  1:316474/238‑1 (MQ=255)

                                                                                                   aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        <  2:410012/167‑1 (MQ=255)

                                                                                                   aTCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCAc                                                                                                                                                                                                        >  1:410012/1‑167 (MQ=255)

                                                                                                        ccacaaaaTAAGCTTCCCCAGCACAATGTTTTCCGCACGTTTGGGCTGGCGGTGAACCCCGGAAAGGGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGGCGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGa                                                                                                                           <  2:931371/233‑1 (MQ=255)

                                                                                                                      tCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGGGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGGTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGGTTTAGACGTTTTGaag                                                                                                                 >  1:931371/1‑234 (MQ=255)

                                                                                                                        gCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCTCGCCGTCGCACATTTGCATCGCGATATAGCCGTTATGAATCtttc                                                                                                            >  1:198788/1‑236 (MQ=255)

                                                                                                                                     tttCCGCCCGTTTGAGCAGGGCGTTAACCCCGGAAAGGGGCGGTTCGTAAAGCGGCCCCTCCGGTACGCCGTTCATCTGTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTc                                                                                               <  2:275251/237‑1 (MQ=255)

                                                                                                                                           cccGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCCCCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGAcc                                                                                        <  1:68273/237‑1 (MQ=255)

                                                                                                                                                      cggtggTTAACCCGGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCa                                                                              <  1:517542/234‑1 (MQ=255)

                                                                                                                                                                ccccGGAAAGCGGGGTTTCGTAAAGCGGCACCACGGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  2:407794/239‑1 (MQ=255)

                                                                                                                                                                ccccGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTTAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGcc                                                                   <  1:788789/239‑1 (MQ=255)

                                                                                                                                                                     gAAAGCGGCGTGTCGTAAAGCGTCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGCATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGCACAGGTGGGTTCCGCCAGTTGGTGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCCCTTTCGCGTCCTCCACCAACTGTTCCAAGTTAACCGCCCAGTc                                                              >  2:518093/1‑239 (MQ=255)

                                                                                                                                                                                     aaaGCGGCACCCCCGGTACGCCGTTCATCTCTTCGA‑GCCGTGAATGGAAAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:644095/238‑1 (MQ=255)

                                                                                                                                                                                     aaaGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACgccgcc                                               <  1:887180/238‑1 (MQ=255)

                                                                                                                                                                                                  cGGTACTCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGt                                 <  1:726797/239‑1 (MQ=255)

                                                                                                                                                                                                         ccGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCCCGTCCTCGACCACCTGTTGCAGGTTACCCCCCCAGTCGTTCGAAACCCCCCCCGCTTTCGGGCGGTGGTaac                           >  2:254285/1‑237 (MQ=255)

                                                                                                                                                                                                              tctcttTTCGG‑GGCGTTATGGGGAAATGTTTAAGGGAAAGGCGTCGGGGATCAGCAGCCCCGAACGGGTGGGGCCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCcca                     <  2:934668/237‑1 (MQ=255)

                                                                                                                                                                                                               cccttttCGC‑GGCGGGAATGGAGAATGTTGAAGGGAAAGCCGTCGGGGTTCCGGACCCCCGAACAGGGGGGTGCCGCCAGTTGATGGACCCTTTTTTTCCCTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccac                    <  2:941945/234‑1 (MQ=255)

                                                                                                                                                                                                                actcgTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCccacc                   <  1:254285/238‑1 (MQ=255)

                                                                                                                                                                                                                    ttCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCTTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGCCGTTCGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACtt               >  1:337331/1‑239 (MQ=255)

                                                                                                                                                                                                                      cGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGCTATAGACGTTATGAATCTTGCCCGCTTTCCGGTCCTCGACCCGCGGTTGCAGGTTACCCGCCCAGTCGTTAGAAACCCCGCCCCGTTTCGGGTGGAGGTAAACGCCACCCACTCCa             >  2:449307/1‑239 (MQ=255)

                                                                                                                                                                                                                      cGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGCCCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCCCCCGGCTTCGGGTGGGGGTAAAccccccccccccca             >  2:356716/1‑233 (MQ=255)

                                                                                                                                                                                                                       gA‑GGGGTGAATGGAGAATGGTGAAGGTAAAGACGGCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:356716/239‑1 (MQ=255)

                                                                                                                                                                                                                        ggggCGTGAATGGATAATGTTGAAGGTAAAGGCGGCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTCTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCaa            <  1:556639/237‑1 (MQ=255)

                                                                                                                                                                                                                                  tGGGGAATGGTTAAGGTAAAGGCGGCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCTGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:456335/239‑1 (MQ=255)

                                                                                                                                                                                                                                  tGGAGATGGTTGAAGGTAAAGCCGTCGGGGATCAGCAGCCCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCa  <  1:147043/239‑1 (MQ=255)

ATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGA‑GGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCCGCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCA  >  NC_000913/2120590‑2121053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A