Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	191	118.8	2757226	85.6%	2360185	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,825,130	G→T	20.8%	intergenic (‑193/+10)	ves ← / ← spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,130	0	G	T	20.8%	138.6 / 8.8	83	intergenic (‑193/+10)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand): ref base G (32/31); new base T (15/3); total (49/34)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGC  >  NC_000913/1824899‑1825365

caGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTAAGGTTTTTGGCTG‑gaa                                                                                                                                                                                                                                              >  1:797045/1‑239 (MQ=255)

               tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTTAGTTTTTTTTTTG‑CAAAATTATTCAGCAGtt                                                                                                                                                                                                                               >  2:1200880/1‑239 (MQ=255)

                                      tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTACGGTTTTTTGCTG‑AAAGATTATTCATCAGTTGCAGG‑CATTTTACCTTTTTcc                                                                                                                                                                                                         >  1:376953/1‑237 (MQ=255)

                                        cccGCCCCCGGCCTTTTTTTTTGTTTTTTACTGTACGGGTAAGCGTGGATGTTTACCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTCACATCTTTTTGAGAAGAAAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTg                                                                                                                                                                                                     <  1:38463/237‑1 (MQ=255)

                                         tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTTAGGTTTTTGCGTG‑AAAGATTTTTTAGCAGTTGCAGG‑CATTTTTCCTTTTTCCGCCgg                                                                                                                                                                                                    >  2:1257382/1‑239 (MQ=255)

                                           gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GTTTCAGTTTTTTGTCTG‑AAAGAATTTTAATACGTTTCAGG‑CCTTTTTCTTTTTTCCGCtcg                                                                                                                                                                                                    >  1:587013/1‑235 (MQ=255)

                                           gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCAACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAACTTTTTTGGCTG‑AAAGATTATTCATCAGTTGCAGG‑CATTTTGCCTGTTGCCGCTGGAc                                                                                                                                                                                                  >  1:1124079/1‑239 (MQ=255)

                                                                   ttATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTAAGTTTTTTGTCTG‑TAAGATTATTTAGCAGTTTGAGG‑CATTTTTCCTTTTGCCTCTGGGCGTTCTTT‑CAGAAGCTT‑CTCaaaaa                                                                                                                                                                        >  2:278358/1‑238 (MQ=255)

                                                                        gTTCAGGTAACCGTGGATGGTTATCGGCACCTCGGGTGGTTTACGTTAGTGGTGATCAGGCTTTTTTTACACCTGTTTGAGAAGTGAAAAGCAA‑A‑CCGCCGATCCTGTCCACCGCATAACTGCAAGGTAGTGGACAAAACCG‑GCGGATGTTAA‑TTTTTTTGCTGAAAAGATATTTAACCATTTGCGGC‑CTTTTTACTTTTTCTCCCTGGAGGTTTTTT‑CAAGCGCTC‑CTAaaaaatcac                                                                                                                                                                    >  2:989785/1‑234 (MQ=255)

                                                                             ggTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTTAATTTTTTTGCTG‑AAAGATTATTCTGCAGTTGCAGG‑CACTTTTCCTTTTTCCCCTGGACGTTCTTC‑TAGACCCTT‑CTCACAATTA‑ACATTaaa                                                                                                                                                             >  2:1226524/1‑239 (MQ=255)

                                                                              gTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTAAGTTTTTTGGCTG‑AAAGAGTATTAAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTATGT‑CCGACGCTT‑TCCCAAAGTA‑GCATTAAAt                                                                                                                                                            >  1:1167278/1‑239 (MQ=255)

                                                                                 aCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTTAGCCGTTGCAAG‑CCTTTTACCTTTTTCCGCTGGACGTTCTTT‑CCGACGCTT‑TTCCAAATTT‑GCCTTTAAtttt                                                                                                                                                         >  1:430643/1‑237 (MQ=255)

                                                                                   cGTGGATGGTTATCGGCAACTCGGGTGATTGACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGAAGTGGACAAGACCGCGGGC‑TCTTCGGTTTTTTCG‑TT‑AAGGATTATTTAGCAGGTGGAGG‑GACTTTCTCTTTTTGCCCTGGGCGTTCTGT‑TAAACCCTC‑CTCAAAAATA‑GAAATAAAATTCtt                                                                                                                                                       >  2:825722/1‑239 (MQ=255)

                                                                                        aTGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGCCAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑AAAGATTTTTTAGCCGTTTCAGG‑CATTTTACCTTTTTCGCCCGGAGGTTCTGT‑CCGAGCGTT‑TTCCAAATTA‑GCATTCAATTGtttttttt                                                                                                                                                  >  1:1275387/1‑237 (MQ=255)

                                                                                             tATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑CAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGTCGTTTTGT‑CAACAGCTT‑CTCACAATTA‑GCATTAAAATCTGTTTTTTGTTcc                                                                                                                                             >  1:32856/1‑239 (MQ=255)

                                                                                                  gacacgtggtggATTTGCTTTGGTGGTGATCGGTTTTTTTTTACACGTGTTAGAGAAGAAAAAAAAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGt                                                                                                                                        <  2:40510/228‑1 (MQ=255)

                                                                                                     aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑GAAGATTATTCAGCAGGTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTTAATTGCTTTTTCTGTTCCGGCGTCA‑g                                                                                                                                    >  1:159469/1‑239 (MQ=255)

                                                                                                      cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCCGG‑CCTTTTACCTTTTTCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCCAAATTA‑GCATTTAATTGCTTTTTCTTTTGCGGCGTCA‑gg                                                                                                                                   >  1:40510/1‑239 (MQ=255)

                                                                                                      cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTAAGTTTTTTGGCTG‑AAAGATTATTCCGCCGGTGCAGG‑CATTTTTCCTTTTTCCGCTGGACGGTCTGT‑CAGAAGCCT‑CTCAAAATTATGATTTAATTTGCTTTTTTTTTTCCgggccagg                                                                                                                                     >  2:1114894/1‑233 (MQ=255)

                                                                                                                 tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGAATATTCAGCAGTTGCAGG‑CCTTTTACCTTTTTCCGCCGGACGTTTTGT‑CAGACGCTT‑CTCAACATTA‑GCATTAAATTTCTTTTTTTTTTTCGCCGGCA‑GGAGGTTG‑Taaaa                                                                                                                       >  1:1357860/1‑238 (MQ=255)

                                                                                                                 tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑CCTTAAGTTTTTTTGCCG‑AAAGATTATTCAGCAGTTTCAGG‑CCTTTTACCTTTTGCCGCTGGACGGTCTGT‑CAGAAGCTT‑CTCCAAATTA‑TCCTTTAATTTCTTTTTCTGTTCCGGCGTCA‑GGTGTTTG‑Taaaa                                                                                                                       >  1:1240299/1‑238 (MQ=255)

                                                                                                                        gtggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGAAGTGGACAAGACCG‑GCGG‑TCTTAAGGTTTTTGGCTG‑CAAAATTATTCAGCAGTTGCAGGCCTTTTTACCTTTT‑CCCCTGGACGGTCTGT‑CTGAAGCTTCCTCAAATTTC‑TC‑TTTAATTTCTTTTTTTTTTTCGGGGGCC‑GGAGTGTG‑TAAATttttttt                                                                                                                >  2:1050140/1‑238 (MQ=255)

                                                                                                                         tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑AAAGATTATTCAACAGTTTCCGG‑CCTTTTACCCTTTTCCGCTTGGAGTTTTGT‑CATACGCGT‑CTCAAAATTA‑GCATTAAATTTCTTTTTTTTTTCCGGCGCCG‑GGCTGTTG‑TAAAtttttt                                                                                                                  >  2:1095947/1‑234 (MQ=255)

                                                                                                                         tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑GAAGAGTATTCAGCAGTTGCAGG‑CATTTTACCTTTTTCCGCTGGGAGTTTTGT‑CAGACGGTT‑CTCAAAATTA‑GCATTAAATTTCTTTTTCTGTTTCGGCGTCA‑GGAAGTTG‑TAAATTTTGt                                                                                                                  >  1:951036/1‑236 (MQ=255)

                                                                                                                              aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTTAGTTTTTTTGCTG‑AAAGATTATTTCGCAGTTGCAGG‑CATTTTTCCTTTTTCCGCTGGGCGTTCTTT‑CAGACCCTT‑CTCAAAATTA‑GCATTTAATTTGTTTTTTTTTTTCTGCGTCC‑GGGTTTTT‑TAAATTTTTTTT‑TGGGtt                                                                                                         >  2:1122507/1‑239 (MQ=255)

                                                                                                                              aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑GAAGATTATTTAGCAGTTTCAGG‑CATTTTACCTTTTTCCGCCGGGCGTTCTGT‑CAGACGCCT‑CTCAAAAATA‑ACCTTTAATTTGTTTTTCTGTTCCCGCGTGAGGGCTTTTT‑TAAATtttttttgtgtt                                                                                                           >  2:61071/1‑233 (MQ=255)

                                                                                                                              aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑AAAGGATATTCAGCAATTTTAGG‑CATTTTTTCCTTTGCCGCTGGTCGTTTCTT‑TAGCACCTT‑CTCAAAATTA‑GCTATAAATTTCTTTTTCTGTTTCTGCGTCC‑GGCAGTTT‑TAAAATTTGTTT‑TGGGtt                                                                                                         >  2:50946/1‑239 (MQ=255)

                                                                                                                                  ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAATTTTTTGGCTG‑AAAAATTATTTAGCAGGTGCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTTT‑CAGACGCTT‑CTCAAAATTA‑GCATTTAATTGCTTTTTCTGTTTCGGCGTCC‑GGATGTTG‑TAAATTTTGTTC‑TGGGtttt                                                                                                       >  1:1228476/1‑236 (MQ=255)

                                                                                                                                    ttttttttACAATTGTTTGAAAAAAAAAAAAAAACA‑CCGCCCATCCTGTCCACCTCATTACTGGAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCAt                                                                                                    <  2:816048/238‑1 (MQ=255)

                                                                                                                                     tttttttACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAAGTTTTTGCCTG‑GAAGATTATTCATCGCTTGGAGG‑CATTTTATCTTTTTCCGCAGGCGGTTTTTT‑TAGACGATT‑GCCCAAATTA‑TCATTAAATTGCTTTTTCTGTTCCTGCGCCA‑GGAGGTTT‑TACATTTTGTGT‑TGGGGTTcctgtt                                                                                                  >  2:741709/1‑235 (MQ=255)

                                                                                                                                        ttttACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGGCGGTCTGT‑CAGACGCCT‑CTCAAAATTA‑GCATTAAATTGCTTTTTTTGTTCCGGCGTCA‑GGGTGTTG‑TAAATTTTGTTC‑TGGGGTTCCATTTgc                                                                                                >  1:138886/1‑238 (MQ=255)

                                                                                                                                                     tGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTAGGTG‑AAAAATTATTCCGCAATTGCACG‑CATTTTACCTTTTTGCGCTGGACGGTCTTT‑CAGACGCTT‑CCCCAAATAA‑GCATTAAATTGGTTTTTTTTTTCCGGGCTCC‑GGTTTTTT‑ATAATTTTTTTT‑TGGGGTTCCCTTGGGGCCCGCA‑TTTTAgg                                                                                 >  2:1336437/1‑238 (MQ=255)

                                                                                                                                                          agaaaaAAGAAA‑T‑CCGGCGGTCCTGTCCACCGGATTTCGGCAAGGTGGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgc                                                                            <  2:32856/239‑1 (MQ=255)

                                                                                                                                                           aagaaaagaaa‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                           <  1:330609/238‑1 (MQ=255)

                                                                                                                                                           aaaaaaaaaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTGTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                           <  1:1174510/236‑1 (MQ=255)

                                                                                                                                                            agaaaaggac‑ACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑GCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGGTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                           <  1:1306048/239‑1 (MQ=255)

                                                                                                                                                             gaaaagaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGTCGTTCTGT‑CAGACGCTT‑CTCCAAATTA‑GCATTAAATTGCTTTTTTTTTTCCGGCGGCC‑GGGTGTTT‑TAAATTTTTTTCTTGGGTTTCATTGGGC‑CCAGCA‑CGTTaagttttgggt                                                                         >  2:760743/1‑229 (MQ=255)

                                                                                                                                                                                 ccTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCg                                                        <  1:278358/238‑1 (MQ=255)

                                                                                                                                                                                  ctttCCCCCGCATTACTGCAAGGTAGTGGACAAGACCG‑GGGG‑TTTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                      <  2:1228476/236‑1 (MQ=255)

                                                                                                                                                                                  cTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                      <  1:1095947/239‑1 (MQ=255)

                                                                                                                                                                                  cTGGCCCCCGCATTACCGCAAGGTAGTGGACAAGGCCG‑GCGG‑TCTTAAGTTTGTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                      <  2:978142/239‑1 (MQ=255)

                                                                                                                                                                                    gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑GAAGATTATTCAGCAGGTGCAGG‑CCTTTTACCTTTTTCCGCTGGACGGTCTGT‑CAAACGCTT‑CTCAAAAATA‑ACCATTAATTTCTTTTTTTTTTCCCGCGTCC‑TGTGGTTT‑TAAAATTTGTTT‑TGGGGTTTCCTGTTCCCCAGCA‑AGGTAGCTTTTCCGTTTTTTTCCCtttttttggttt                                                    >  2:921162/1‑232 (MQ=255)

                                                                                                                                                                                     tcccacGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                   <  1:1336437/234‑1 (MQ=255)

                                                                                                                                                                                          ccagttACTGCAAGGTAGGGGACAAGACTG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGATTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                              <  1:908689/235‑1 (MQ=255)

                                                                                                                                                                                          cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑GAAGATTATTTAGCAGTTGCAGG‑CCTTTTTCCCTTTTCCCCCGGACGTTCTTT‑CCGACGCTT‑TTCAAAATTA‑GACTTTAATTGCTTTTTTTGTTCCCGCGTCC‑GGATGTTT‑TAAATTTTGTTT‑TTGGTTTTCCTTTTCGCCAGCC‑TTTTGACTTTTGCCTGTTTTTCCATTTTTTCGATTTGCGCt                                               >  2:582178/1‑238 (MQ=255)

                                                                                                                                                                                          cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATTTGCGCCAGCA‑TGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCtcggct                                               >  1:1114478/1‑233 (MQ=255)

                                                                                                                                                                                          cGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑CCTTTTGTTTTTTGGGTG‑AAAGGTTATTCAGCAGATGGAGG‑CATTTTTCCTTTTTCCGCTGGCCGGTCTGT‑CAGACACTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTTTGTTTCGGCGTCA‑GGGTGTTG‑TAAATTTTTTTT‑TGGGTTTTCATTGTCGCCCGCA‑TGTTAACTTTGAGCTGTTTTTCCATTTTTTTGATCTgcggt                                               >  2:932770/1‑236 (MQ=255)

                                                                                                                                                                                           gCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                             <  1:574006/239‑1 (MQ=255)

                                                                                                                                                                                               tACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CCTTTTACCTTTTTCCGCTGGACGTTCTGT‑TAAACGCTT‑CTCCAAATTA‑GCATTTAATTGCTTTTTTTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTTTTT‑TGGGTTTTCTTTTGGGCCCGCA‑TGTTAGCTTTGCGCTGTTTTTCCATTTTTTCGATTTGCGCTTCCGCt                                         >  2:471777/1‑239 (MQ=255)

                                                                                                                                                                                                 cTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGGTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTTCATTTTTTCGGTCTGCGCTTCAGCttt                                       >  1:753614/1‑239 (MQ=255)

                                                                                                                                                                                                    cAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGAAGTTGCAGG‑CATTTTAACTTTTGCCGCTGGACGTTTTTT‑CAGAAGCTT‑CTCCAAATTA‑GCATTAAATTTCTTTTTCTTTTCCGGCGTCA‑GGAGGTTG‑TAAATTTTGTTC‑TGGGTTTCCCTGTGCGCC‑GCACTGTTAGCTTTGCGCTGTTCTTCCCTTTTTTCGATCTGCGCTTCCGCtttt                                      >  1:1155205/1‑237 (MQ=255)

                                                                                                                                                                                                     aaGGTAGTGGACAAGGCCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                   <  1:541928/239‑1 (MQ=255)

                                                                                                                                                                                                      aGGTAGTGGACTAGACCG‑GCTG‑TCTTAAGTTTTTTGGCTG‑AAAAATTATTCAGCAGTTGCAGG‑CATTTTACCTTTGGGCGGTGGACGTTCTGT‑CAGGCGCTT‑CTCAAAATTA‑GCATGAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTTTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                   <  1:1089417/238‑1 (MQ=255)

                                                                                                                                                                                                       ggTAGTGGACGAGACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGTCGCCGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTTAATTGCTTTTTTTGTTCCGGCGTCC‑GGATGTTG‑TAAATTTTGTTC‑TTGGGTTCCATGTGCGCCAGCC‑AGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGATTTTCGCTTTAGCTTTTACttt                                 >  1:197230/1‑239 (MQ=255)

                                                                                                                                                                                                         tAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCAttttt                                                           <  1:850627/211‑1 (MQ=255)

                                                                                                                                                                                                          tgTGGACAAGACCG‑GCGG‑TCTTAAGTTTTGTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCGGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTAt                               <  1:435126/237‑1 (MQ=255)

                                                                                                                                                                                                            tGGACAAGGCCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGa                            <  1:471777/239‑1 (MQ=255)

                                                                                                                                                                                                            tGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑TTCAAAATTA‑GCATTAAATTGCCTTTTTTGTTCCGGCGTCA‑GGATGTTG‑TAAACTTTGTTC‑TTGGTTTCCATGTCGGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCCTTTTTTCGATTCTCCCTTCCCCCTTTACTTTTTcta                            >  1:1043413/1‑237 (MQ=255)

                                                                                                                                                                                                            tGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGGTGCAGG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTTAATTGCTTTTTTTGTTCCGGCGTCA‑GGGTGTTG‑TAAATTTTGTTT‑TTGGTTTTCCTTTGCGCCCGCC‑TGTTTGCTTTTGGCTGTTTTTTCCTTTTTTCGACTTGCGCTTCACTTTTTACTTTTTCGa                            >  2:1124232/1‑239 (MQ=255)

                                                                                                                                                                                                            tGGACAAGACCG‑GCGG‑TCTTAAGGTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGCTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTTTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTTTTC‑TGGGGTTCCATGTGCGCCAGCA‑TGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCTGCGCTTCCGCTTTTACTTTATCGa                            >  1:744947/1‑239 (MQ=255)

                                                                                                                                                                                                              gACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGcc                                                                                             <  1:451964/173‑1 (MQ=255)

                                                                                                                                                                                                                 aaGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTT‑TGGGTTTTCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGATCTGCGCTTCAGCTTTTACTTTTTCGAAGGt                        >  1:309303/1‑238 (MQ=255)

                                                                                                                                                                                                                   gACCG‑GCGG‑TCTTAAGTTTTTTTGCTG‑AAAGATTATTTAGCCGGTGCACG‑CATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTTAATTTATTTTTTTTTTTCTGCGTCA‑GGAGGTTG‑TAAATTTTGTTT‑TTGGTTTCCCTTTTTGCCAGCC‑TGTTAGCTTTTGGGTGTTTTTCCTTTTTTTCGATCTGCCCTTCCGCTTTTTTTTTTTCGTAggatt                      >  2:347021/1‑235 (MQ=255)

                                                                                                                                                                                                                   gACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCCTGTGCGCCATAC‑TGTTACCTTTTGGCTGTTTTTCCATTTTTGCGATTTGCGCTTCAGCTTTTTCTTTATTGAAGGAAt                      >  1:162436/1‑238 (MQ=255)

                                                                                                                                                                                                                   gACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGAGGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTTCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATc                     >  1:794645/1‑239 (MQ=255)

                                                                                                                                                                                                                      cg‑gcgg‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                  <  1:1122507/239‑1 (MQ=255)

                                                                                                                                                                                                                      cg‑gccg‑TCTTAAGTTTTTTTGCTG‑AAAGATTATTCAGGAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CGCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                  <  1:302938/239‑1 (MQ=255)

                                                                                                                                                                                                                         gcgg‑TCTTAAGTTTTTTTGGTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTT‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGGGGTTGTTAATTTTTTTTT‑TGGGTTTCCATTTTCGCCACCC‑TTTTAGCTTTTCGCGTTTTTTCCCTTTTTTCGATTTTCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTg                 >  2:141695/1‑239 (MQ=255)

                                                                                                                                                                                                                              tCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa             <  1:136085/238‑1 (MQ=255)

                                                                                                                                                                                                                               ttttaGTTTTTTGGCTT‑AAAGATTATTCCGGATTTGCAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  2:197230/235‑1 (MQ=255)

                                                                                                                                                                                                                               tttagtgtttttGTCTT‑AAAGATTTTTCAGCAGGTGGAGG‑CGTTTTTCCGTTTGCCTGTGGAC‑TTTTGTGCAGACGCTT‑CTTAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGGCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  2:1275387/232‑1 (MQ=255)

                                                                                                                                                                                                                               ctttatttttttGGTGT‑AAAGTTTATTCAGCAGTTGTAGG‑CATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCTGCA‑GGATGTTG‑TAAATTTTGTTC‑GGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  2:1378050/233‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGTTTTTTTGCTG‑AAAGATTATTCAGCAGGTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CGGACGATT‑CTCAAAATTA‑TCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:664586/239‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGTTTTTTGGCTT‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:932770/239‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGTTTTTTGGCTG‑AAAGGTTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:72366/239‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTTTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:582178/239‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:146550/239‑1 (MQ=255)

                                                                                                                                                                                                                               cTTAAGGTTTTTGGCTG‑AAAGGTTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg           <  1:1085637/239‑1 (MQ=255)

                                                                                                                                                                                                                                    gTTTTTTTGTTT‑AAAGATTATTAAGCGGTTGCAGG‑CATTTTACCTTTTGCCGCTGGCCGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTTTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTc      <  2:1124079/239‑1 (MQ=255)

                                                                                                                                                                                                                                      tttttGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTc      <  1:61071/237‑1 (MQ=255)

                                                                                                                                                                                                                                        tttGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGCTGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCagga  >  2:535468/1‑236 (MQ=255)

                                                                                                                                                                                                                                        tttGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTTTTCCATTTTTGCGGTCTGCGGTTCAGCTTTTACTTTATTGAAGGTATCGTTGCCAaagattcatagc  >  2:739890/1‑232 (MQ=255)

                                                                                                                                                                                                                                        tttGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTGG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAGGGGATCCCTGGCAATGATGTccg    >  1:1378050/1‑235 (MQ=255)

CAGATTCACCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCG‑GCGG‑TCTTAAGTTTTTTGGCTG‑AAAGATTATTCAGCAGTTGCAGG‑CATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAGACGCTT‑CTCAAAATTA‑GCATTAAATTGCTTTTTCTGTTCCGGCGTCA‑GGATGTTG‑TAAATTTTGTTC‑TGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGC  >  NC_000913/1824899‑1825365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A