Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 578,118 A→C 37.8% V4V (GTA→GTC)  rzpD → DLP12 prophage; putative murein endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913578,1180AC37.8% 81.4 / 59.1 69V4V (GTA→GTC) rzpDDLP12 prophage; putative murein endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (23/20);  new base C (12/14);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

TTCGCAAAATAAACCAGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAAT  >  NC_000913/577971‑578258
                                                                                                                                                   |                                                                                                                                            
ttcGCAAAATAAACCAGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAAAGGAAAGGCCTGATGACACGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTaa                                                                                                                                             >  5:198033/1‑149 (MQ=255)
     aaaaTAAACCAGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGCAATACGCTGGCGGGAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACcgc                                                                                                                                         >  5:105064/1‑148 (MQ=255)
        aTAAACCAGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGAtt                                                                                                                                     <  7:192581/149‑1 (MQ=255)
               aGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGAGGACTCGTCGTGAGATTGCGCGTGCAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCg                                                                                                                              >  1:278439/1‑149 (MQ=255)
                   cGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGctct                                                                                                                          <  3:28332/149‑1 (MQ=255)
                         cAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTAt                                                                                                                    >  3:311577/1‑149 (MQ=255)
                          aaaGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATc                                                                                                                   <  3:247801/149‑1 (MQ=255)
                           aaGGCGCATGTGATCAGCTGGGTCGATGGACATTCGCTGGCTGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTATCCGCGATTATATCCGCTCTGATTATCt                                                                                                                  <  6:198033/149‑1 (MQ=255)
                             ggCGCATGTGTTCAGCTGCGTCGATGGAGTTATGCTGGCGGTAAGCATTGGAAATGCCTGATGACTCGTCGTGAGATTTTGCGTGAATTCTGTTTGTGGGGGCAACAGTGTGCAGAGTAACGGCGATTATATCCGCTCTGATTATCTGc                                                                                                                <  3:65863/149‑1 (MQ=255)
                                      gATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGAt                                                                                                                                      >  8:303226/1‑118 (MQ=255)
                                      gATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGAt                                                                                                                                      <  7:303226/118‑1 (MQ=255)
                                       aTCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCa                                                                                                      <  4:311577/149‑1 (MQ=255)
                                          aGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTAt                                                                                                                    <  7:105390/132‑1 (MQ=255)
                                          aGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTAt                                                                                                                    >  8:105390/1‑132 (MQ=255)
                                              gCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGt                                                                                                >  7:274063/1‑148 (MQ=255)
                                                   gcgggACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATgg                                                                                           <  6:35758/145‑1 (MQ=255)
                                                     tGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCg                                                                                        >  8:352744/1‑149 (MQ=255)
                                                        aCATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTc                                                                                     <  2:9859/149‑1 (MQ=255)
                                                          aTACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGCCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCaa                                                                                   >  7:103696/1‑149 (MQ=255)
                                                           tACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTTTCATGGGCGGTCAAt                                                                                  >  1:199497/1‑149 (MQ=255)
                                                                     gTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGAt                                                                                                                                      <  6:370771/87‑1 (MQ=255)
                                                                     gTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGAt                                                                                                                                      >  5:370771/1‑87 (MQ=255)
                                                                     gTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGt                                                                         >  5:123472/1‑148 (MQ=255)
                                                                      tAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGc                                                                                         <  8:117125/131‑1 (MQ=255)
                                                                      tAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGc                                                                                         >  7:117125/1‑131 (MQ=255)
                                                                          cAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAAc                                                                   >  5:133371/1‑149 (MQ=255)
                                                                               gAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAAt                                                              <  8:274063/149‑1 (MQ=255)
                                                                                 aaGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACGCAATCg                                                            >  7:220323/1‑149 (MQ=255)
                                                                                    gCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATAATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGcc                                                          >  4:317541/1‑148 (MQ=255)
                                                                                        gatgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGAATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACa                                                      >  6:187840/1‑148 (MQ=255)
                                                                                         atgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACaaa                                                    <  6:123472/149‑1 (MQ=255)
                                                                                                gtcgtGAGATTGAGCGTGAAGTCTCTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGATCAATAATTAACTTGATAAATCAATAGCCTACAAAGTCAAgc                                             >  7:353498/1‑149 (MQ=255)
                                                                                                         ttGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCa                                                                                <  5:319191/105‑1 (MQ=255)
                                                                                                         ttGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCa                                                                                >  6:319191/1‑105 (MQ=255)
                                                                                                         ttGAGCGTGAAGTCTGTTTGTGCCGCCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTCATTATCTGCATCATCGTCAGCCTGTCATGGGCGGGCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCACCGCGACaaa                                     >  5:4801/1‑148 (MQ=37)
                                                                                                                tGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGc                                >  3:255292/1‑146 (MQ=255)
                                                                                                                  aaGTCTGTTTGTTGGGGCAACAGGGAGCAGAGTCACCGCGATTATATACGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCaga                            <  6:105064/148‑1 (MQ=255)
                                                                                                                    gtattgttGTGGGGGCAACAGTGAGCAGAGTAACCGAGAGTATATCCGCTCTGATTATCTGCATCATCTTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGaa                         <  2:199497/143‑1 (MQ=25)
                                                                                                                               ggggCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc              <  4:255292/149‑1 (MQ=255)
                                                                                                                                  gCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGaa           <  5:148594/149‑1 (MQ=255)
                                                                                                                                   gatgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGCTTATCTGCATCATCGTCTGCCTGTCATGGTCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAc          >  2:272482/7‑149 (MQ=11)
                                                                                                                                    atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGc                                <  3:203957/121‑1 (MQ=11)
                                                                                                                                    atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                              <  3:315251/123‑1 (MQ=11)
                                                                                                                                    atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                              >  4:315251/6‑128 (MQ=11)
                                                                                                                                     tgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCCTCGTCTGCCTGTCATGGGCGGTTTATCATTCCCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgc        >  3:300436/5‑149 (MQ=11)
                                                                                                                                      gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                            <  2:183910/125‑1 (MQ=11)
                                                                                                                                      gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                            >  1:183910/4‑128 (MQ=11)
                                                                                                                                      gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                        <  1:217212/129‑1 (MQ=11)
                                                                                                                                      gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                        >  2:217212/4‑132 (MQ=11)
                                                                                                                                      cAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAAcgcg       >  7:344002/1‑149 (MQ=255)
                                                                                                                                       tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATACCGGCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgcg       <  6:191777/146‑1 (MQ=11)
                                                                                                                                       tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                              <  5:105395/123‑1 (MQ=11)
                                                                                                                                       tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                              >  6:105395/3‑125 (MQ=11)
                                                                                                                                        gTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCgg      >  6:174608/1‑148 (MQ=255)
                                                                                                                                         tGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATTGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGc     <  6:109236/148‑1 (MQ=11)
                                                                                                                                         tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                           <  4:311728/126‑1 (MQ=11)
                                                                                                                                         tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                           >  3:311728/1‑126 (MQ=11)
                                                                                                                                         tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                <  4:230302/137‑2 (MQ=11)
                                                                                                                                         tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                >  3:230302/1‑136 (MQ=11)
                                                                                                                                          gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                     <  7:210068/131‑1 (MQ=11)
                                                                                                                                          gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                     >  8:210068/1‑131 (MQ=11)
                                                                                                                                          gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                <  2:218203/136‑2 (MQ=11)
                                                                                                                                           aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGc                                                                 >  3:222005/1‑86 (MQ=11)
                                                                                                                                           aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGc                                                                 <  4:222005/86‑1 (MQ=11)
                                                                                                                                           aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                           >  5:6182/1‑124 (MQ=11)
                                                                                                                                           aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                           <  6:6182/124‑1 (MQ=11)
                                                                                                                                           aGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAAt  <  8:220323/149‑1 (MQ=255)
                                                                                                                                            gCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAgcgc                                           >  8:65908/1‑107 (MQ=11)
                                                                                                                                              agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCTGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc              <  6:91822/134‑1 (MQ=255)
                                                                                                                                              agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc              >  5:91822/1‑134 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TTCGCAAAATAAACCAGGGCGATATCAAAGGCGCATGTGATCAGCTGCGTCGCTGGACATACGCTGGCGGTAAGCAATGGAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACGCAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAAT  >  NC_000913/577971‑578258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A