Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 579,127 T→C 100% intergenic (‑234/+57) borD ← / ← ybcV DLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,1270TC98.7% 10270.5 / 31.5 3609intergenic (‑234/+57)borD/ybcVDLP12 prophage; putative lipoprotein/DLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base C (1743/1792);  total (1774/1835)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 3875 total aligned reads displayed.

ATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTG  >  NC_000913/578980‑579273
                                                                                                                                                   |                                                                                                                                                  
    ttAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTTCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACATTGTTTAGCGCTTACAcgtattg                                                                                                                                              >  4:211953/1‑149 (MQ=255)
    ttAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACAcgtattg                                                                                                                                              >  6:27596/1‑149 (MQ=255)
       atatTTCTTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGACCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGCTGCACACAGGGTTTAGCGCGTACAcgtattgcat                                                                                                                                           <  5:334147/149‑1 (MQ=255)
       atatTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACAcgtattgcat                                                                                                                                           >  4:28077/1‑149 (MQ=255)
        tatttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACgtattgcatt                                                                                                                                          <  6:16427/149‑1 (MQ=255)
        tatttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACgtattgcatt                                                                                                                                          <  3:216647/149‑1 (MQ=255)
          tttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGGGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGAGCGTACACGTATTGcattat                                                                                                                                        >  1:27896/1‑149 (MQ=255)
          tttattAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGCCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGcattat                                                                                                                                        <  2:10942/149‑1 (MQ=255)
           ttattaATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCAATATg                                                                                                                                       >  1:100015/1‑149 (MQ=255)
               taATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCa                                                                                                                                    <  4:25077/148‑1 (MQ=255)
                aaTGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCTGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCaa                                                                                                                                   <  8:136474/148‑1 (MQ=255)
                aaTGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCACTTACGGGGAACTAGTCTGCGTGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCa                                                                                                                                    >  2:302584/1‑147 (MQ=255)
                 atgtatgtCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAacg                                                                                                                                 >  1:162354/1‑148 (MQ=255)
                 atgtatgtCAGGTGCGAGGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGGGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAac                                                                                                                                  <  2:164701/148‑1 (MQ=255)
                       gtcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCgg                                                                                                                           >  2:140294/3‑149 (MQ=255)
                        tcAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGt                                                                                                                          >  5:52364/2‑149 (MQ=255)
                         cAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTg                                                                                                                         >  1:35174/1‑149 (MQ=255)
                               cGATGAATCGTCATTGTCTTCCCGGATTAACTATGGCCACCGCCCTGCCGGGGAAGTTCTCTGGGGGAGTGTCCGGGAATAATT‑ATAACGATGCACACCGGGTTTAGCGCGTAGACGTATTGCATTTTGCCAACGCCCCGGTGCTGaga                                                                                                                   <  8:301827/149‑3 (MQ=255)
                                 aTGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACg                                                                                                                 <  4:61291/149‑1 (MQ=255)
                                  tGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACg                                                                                                                 >  4:335816/1‑148 (MQ=255)
                                    aaTCGTCATTGTATTCCCGTATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCAGGGGCTGACACGga                                                                                                               <  5:142137/148‑1 (MQ=255)
                                     aTCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACgg                                                                                                                >  6:131611/1‑146 (MQ=255)
                                      tCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGacac                                                                                                                  >  7:55217/1‑143 (MQ=255)
                                        gTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACAcgtatt                                                                                                                                               <  8:194869/112‑1 (MQ=255)
                                        gTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGaaa                                                                                                          >  4:365793/1‑149 (MQ=255)
                                               tATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACg                                                                                                   >  6:70458/1‑149 (MQ=255)
                                               tATTCCCGGATTAACTATGTCCACAGACCTGACGGGGAACTTCACTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTAAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGAGCTGACACGAAAGAAACCGGACg                                                                                                   >  8:356185/1‑149 (MQ=255)
                                                 ttCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGtt                                                                                                 >  7:122354/1‑149 (MQ=255)
                                                   cccGGTTTAACTATGTCCACAGCACTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACTGGGTTTAGCGCGTACTCGTATTGCATTATGCCAACGCGCCGGTGCTGACACGGAAGAAACCGGACGTTa                                                                                                <  4:144770/148‑1 (MQ=255)
                                                   cccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGATTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTa                                                                                                <  7:182785/148‑1 (MQ=255)
                                                   cccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTa                                                                                                <  5:78293/148‑1 (MQ=255)
                                                   cccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                               >  5:248407/1‑149 (MQ=255)
                                                    ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                               >  6:30315/1‑148 (MQ=255)
                                                    ccGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAt                                                                                               >  3:83651/1‑148 (MQ=255)
                                                       gactAACTATGTCCACAGCACTGACGGTTAACTTCTCGGCGGGAGGGTCCGTTAATAATT‑AAAACGATGCACCCAGGGGTTAGCGCTTACACGTATTTCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGAtt                                                                                           <  8:274627/146‑1 (MQ=255)
                                                         ttAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTa                                                                                         <  3:243329/149‑1 (MQ=255)
                                                            aCTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGa                                                                                                                     <  6:345677/118‑1 (MQ=255)
                                                            aCTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCg                                                                                      >  2:85276/1‑149 (MQ=255)
                                                            aCTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCg                                                                                      >  1:217570/1‑149 (MQ=255)
                                                             cTATGTCCACAGCCCTGACGGGGAAATTATCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGCGTTTAGCGCGTACACGTATTGCTTTATGCCCAAGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATAATTTAGc                                                                                       >  7:336866/1‑147 (MQ=255)
                                                              tATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGAc                                                                                                    >  6:222036/1‑133 (MQ=255)
                                                               aTGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGCTGCACACAGGGTGTAGCGCGTACACGTATTGCATTATGCCAACTCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTTAGCggg                                                                                    >  7:82648/1‑146 (MQ=255)
                                                                tGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTg                                                                                    <  5:27596/147‑1 (MQ=255)
                                                                   ccACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                  >  6:19225/1‑146 (MQ=255)
                                                                   ccACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                  >  3:130146/1‑146 (MQ=255)
                                                                    cacaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                  >  5:293778/1‑145 (MQ=255)
                                                                    cacaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGa                                                                                  >  3:19206/1‑145 (MQ=255)
                                                                     acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAt                                                                             >  4:19479/1‑149 (MQ=255)
                                                                     acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAt                                                                             >  4:310061/1‑149 (MQ=255)
                                                                     acaGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAt                                                                             >  1:325194/1‑149 (MQ=255)
                                                                       aGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAttt                                                                           <  4:88767/149‑1 (MQ=255)
                                                                          ccTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtgtg                                                                        <  4:11230/149‑1 (MQ=255)
                                                                            tGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTtgtg                                                                        >  7:242638/1‑147 (MQ=255)
                                                                              aCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTa                                                                      <  5:222886/147‑1 (MQ=255)
                                                                              aCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAgt                                                                    >  8:383143/1‑149 (MQ=255)
                                                                               cGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑ATAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAgtg                                                                   <  2:351649/149‑1 (MQ=255)
                                                                                 gggAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGtt                                                                 <  8:256431/149‑1 (MQ=255)
                                                                                  ggAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTc                                                                <  3:365950/149‑1 (MQ=255)
                                                                                   gAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTACTGTTCt                                                               >  2:245228/1‑149 (MQ=255)
                                                                                   gAACTTCTCTGCGGGAGGGTCCTGGAAAAATT‑AACACGAGGCACACAGGGTTTAGCGCGTAAACGTATTGCATTATGCCATCGCCCCGGTGCTGACACGGAAGAAACGGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCt                                                               <  6:94339/149‑1 (MQ=255)
                                                                                      cTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGaa                                                            <  8:163243/149‑1 (MQ=255)
                                                                                      cTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGaa                                                            >  3:54096/1‑149 (MQ=255)
                                                                                       ttCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAt                                                           >  7:211228/1‑149 (MQ=255)
                                                                                       ttCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAt                                                           <  1:8459/149‑1 (MQ=255)
                                                                                        tctcTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATg                                                          >  1:378248/1‑149 (MQ=255)
                                                                                         ctctGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATg                                                          <  7:306910/148‑1 (MQ=255)
                                                                                          tctGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTa                                                                      >  7:151168/1‑135 (MQ=255)
                                                                                            tGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctct                                                      >  3:167556/1‑149 (MQ=255)
                                                                                            tGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGct                                                        <  8:114000/147‑1 (MQ=255)
                                                                                              cGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGTGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctct                                                      <  5:194936/147‑1 (MQ=255)
                                                                                              cGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctct                                                      >  5:360436/1‑147 (MQ=255)
                                                                                              cGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGctc                                                       <  5:173316/146‑1 (MQ=255)
                                                                                                       ccGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCACCGGAGCTGACACGGAAGAAACAGGACGTTATGAt                                                                                            >  2:274527/1‑100 (MQ=255)
                                                                                                         gggAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTaa                                         >  2:377793/1‑149 (MQ=255)
                                                                                                          ggAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAt                                        <  6:249622/149‑1 (MQ=255)
                                                                                                           gAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAt                                        >  7:288461/1‑148 (MQ=255)
                                                                                                           gAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAt                                        >  8:46741/1‑148 (MQ=255)
                                                                                                             ataatT‑AAAACGATGCACACAGGGTTTAGCGCGTACACGTATTGCTTTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGaa                                     <  6:10524/149‑1 (MQ=255)
                                                                                                                 attaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAAtt                                   <  8:224013/145‑1 (MQ=255)
                                                                                                                 attaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTa                                  >  8:7147/4‑149 (MQ=255)
                                                                                                                  ttaaaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCAGTTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAATTGTGTAGTGTTCTGAATTCTCTCAGATAATAGTAATGAATTAt                                 >  1:133696/3‑149 (MQ=255)
                                                                                                                    aaaaCGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGATGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTATAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATc                                >  4:171710/1‑148 (MQ=255)
                                                                                                                     aaaCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCaa                              <  3:331802/149‑1 (MQ=255)
                                                                                                                       aCGATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCTGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAg                            <  1:244472/149‑1 (MQ=255)
                                                                                                                         gATGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGt                          <  1:303344/149‑1 (MQ=255)
                                                                                                                           tGCACACAGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGta                         >  3:299205/1‑148 (MQ=255)
                                                                                                                             cacacaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGtata                       <  7:268362/148‑1 (MQ=255)
                                                                                                                              acacacGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATa                                             >  6:383997/1‑125 (MQ=255)
                                                                                                                                acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                      >  2:54715/1‑146 (MQ=255)
                                                                                                                                acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAg                      <  1:54715/146‑1 (MQ=255)
                                                                                                                                acaGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTaa                   >  2:323804/1‑149 (MQ=255)
                                                                                                                                 caGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAat                  <  7:387730/149‑1 (MQ=255)
                                                                                                                                  aGGGTTTAGCGCGTACACGTATTGCATTATGCCAACGACCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAata                 >  2:218902/1‑149 (MQ=255)
                                                                                                                                  aGGGTTTAGCGCGTACACGTATTGCATTATGACAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATAAAAGGTATAGTAata                 >  5:113616/1‑149 (MQ=25)
                                                                                                                                    ggTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATg                                                          >  8:327055/1‑106 (MQ=255)
                                                                                                                                    ggTTTAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATc               <  7:359431/149‑1 (MQ=255)
                                                                                                                                      tttAGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtt             >  3:107303/1‑149 (MQ=255)
                                                                                                                                         aGCGCGTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCtttt           <  2:192665/148‑1 (MQ=255)
                                                                                                                                             cgTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTaaa                                               <  1:188798/108‑1 (MQ=255)
                                                                                                                                              gTACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGtt      >  3:275260/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTACATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTG  >  NC_000913/578980‑579273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 2117 total aligned reads displayed.
TCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTA‑CATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATA  >  NC_000913/578976‑579277
                                                                                                                                                        |                                                                                                                                                      
   TATTGATAATATTTATTAATGTATGTCAGCTGCGATGAATCGTCATACTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGAAGCCCACACGGATTAGCGCGTA‑CACGT                                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11610:1450:18552/1‑150 (MQ=60)
    ATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCTCACAGGGTTTAGCGCGTA‑CACGTAT                                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21503:26491:11714/1‑151 (MQ=60)
    ATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTAT                                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11611:21172:1204/151‑1 (MQ=60)
    ATTGTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTA                                                                                                                                                     <  NB501016:230:HFVJLAFXY:2:11111:13953:16002/150‑1 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:2:11103:8931:6091/151‑1 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21406:10473:8671/151‑1 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:2:11311:11629:1365/1‑151 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTG                                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11503:6029:11457/150‑1 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTG                                                                                                                                                  <  NB501016:230:HFVJLAFXY:1:11101:13723:9061/150‑1 (MQ=60)
       GTTAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTG                                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11601:6702:7848/150‑1 (MQ=60)
         TAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCAT                                                                                                                                               >  NB501016:230:HFVJLAFXY:1:11306:17680:3898/1‑151 (MQ=60)
         TAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCA                                                                                                                                                <  NB501016:230:HFVJLAFXY:4:11510:6055:9900/150‑1 (MQ=60)
         TAATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCAAATCCCTGACGGGGAACTTCTCTGAGGGTGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCAT                                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11612:14555:1224/1‑151 (MQ=60)
          AATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATT                                                                                                                                              <  NB501016:230:HFVJLAFXY:3:21605:15589:19459/151‑1 (MQ=60)
          AATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATT                                                                                                                                              >  NB501016:230:HFVJLAFXY:1:11209:20253:9552/1‑151 (MQ=60)
          AATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCAT                                                                                                                                               <  NB501016:230:HFVJLAFXY:1:11310:4308:16369/150‑1 (MQ=60)
          AATATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCAT                                                                                                                                               <  NB501016:230:HFVJLAFXY:1:21109:1640:9517/150‑1 (MQ=60)
          AATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTA‑CATGTATTGTA                                                                                                                                                <  NB501016:230:HFVJLAFXY:4:11604:3045:8950/150‑1 (MQ=60)
            TATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTAT                                                                                                                                            >  NB501016:230:HFVJLAFXY:2:11106:4784:13370/1‑151 (MQ=60)
             ATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATG                                                                                                                                           <  NB501016:230:HFVJLAFXY:4:11405:5801:19502/151‑1 (MQ=60)
             ATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTAT                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11506:7872:6610/150‑1 (MQ=60)
             ATTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCAACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATG                                                                                                                                           >  NB501016:230:HFVJLAFXY:4:11410:25535:2265/1‑151 (MQ=60)
              TTTATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTA                                                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11102:3009:14979/1‑140 (MQ=60)
                TATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AACACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATTCC                                                                                                                                         >  NB501016:230:HFVJLAFXY:1:11110:22669:2529/1‑150 (MQ=60)
                TATTAATGTATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAAATATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCC                                                                                                                                         <  NB501016:230:HFVJLAFXY:3:21401:24599:16458/150‑1 (MQ=60)
                         ATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCCCAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGG                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11511:5425:10214/1‑151 (MQ=60)
                         ATGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAAATTCTCTGCGTGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCACGG                                                                                                                               >  NB501016:230:HFVJLAFXY:3:21408:25381:20066/1‑151 (MQ=60)
                           GTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTG                                                                                                                             >  NB501016:230:HFVJLAFXY:4:11404:8529:18464/1‑151 (MQ=60)
                            TCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGC                                                                                                                            >  NB501016:230:HFVJLAFXY:1:21102:13280:13737/1‑151 (MQ=60)
                            TCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGC                                                                                                                            >  NB501016:230:HFVJLAFXY:4:21409:22786:16042/1‑151 (MQ=60)
                             CAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGCCGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCT                                                                                                                           >  NB501016:230:HFVJLAFXY:3:11402:17869:10734/1‑151 (MQ=60)
                               GGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTG                                                                                                                          >  NB501016:230:HFVJLAFXY:2:21106:26185:9080/1‑150 (MQ=60)
                                GTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGATTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGAC                                                                                                                        <  NB501016:230:HFVJLAFXY:4:11511:6722:19935/151‑1 (MQ=60)
                                 TGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGAC                                                                                                                        >  NB501016:230:HFVJLAFXY:1:11205:13281:14355/1‑150 (MQ=60)
                                 TGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGAC                                                                                                                        >  NB501016:230:HFVJLAFXY:1:21302:12762:9176/1‑150 (MQ=60)
                                   CGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11109:19233:10351/151‑1 (MQ=60)
                                    GATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACG                                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11312:20110:13024/1‑134 (MQ=60)
                                     ATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGG                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11505:3191:6842/1‑150 (MQ=60)
                                       GAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTTTTGCATTATGCCAACGCCCCGGTGCTGACACGGAAG                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11502:19428:10750/151‑1 (MQ=60)
                                       GAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGTCAACGCCCCGGTGCTGACACGGAAG                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11402:6599:19213/151‑1 (MQ=60)
                                        AATCGTCATTGTATTCCCGGATTAACTATGTCCACGGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGA                                                                                                                   >  NB501016:230:HFVJLAFXY:4:21510:7886:12883/1‑148 (MQ=60)
                                         ATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑GACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGA                                                                                                                >  NB501016:230:HFVJLAFXY:1:11305:2721:6048/1‑150 (MQ=60)
                                            GTCATTGTATTCCCGGATTAACTATTTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACC                                                                                                            <  NB501016:230:HFVJLAFXY:3:21508:19748:1851/151‑1 (MQ=60)
                                            GTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACC                                                                                                            >  NB501016:230:HFVJLAFXY:1:21208:6341:7265/1‑151 (MQ=60)
                                             TCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGGA‑CACGTAATGCAGTATGCCAACGCCCCGGGGCTGACACGGAAGAAACCG                                                                                                           >  NB501016:230:HFVJLAFXY:4:21506:3982:19814/1‑151 (MQ=60)
                                              CATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGG                                                                                                          >  NB501016:230:HFVJLAFXY:2:11112:13253:9245/1‑151 (MQ=60)
                                              CATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGG                                                                                                          <  NB501016:230:HFVJLAFXY:4:21502:14633:11639/151‑1 (MQ=60)
                                                  GTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGT                                                                                                      >  NB501016:230:HFVJLAFXY:3:11607:20958:15607/1‑151 (MQ=60)
                                                  GTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACG                                                                                                       <  NB501016:230:HFVJLAFXY:4:21602:4272:12394/150‑1 (MQ=60)
                                                  GTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACG                                                                                                                     >  NB501016:230:HFVJLAFXY:1:21103:3405:19849/1‑136 (MQ=60)
                                                   TATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCTTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGGT                                                                                                     >  NB501016:230:HFVJLAFXY:2:21105:24117:16483/1‑151 (MQ=60)
                                                   TATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTT                                                                                                     >  NB501016:230:HFVJLAFXY:1:21311:4411:16548/1‑151 (MQ=60)
                                                    ATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTA                                                                                                    <  NB501016:230:HFVJLAFXY:3:21504:13701:10423/151‑1 (MQ=60)
                                                     TTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCGCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTAT                                                                                                   >  NB501016:230:HFVJLAFXY:3:21606:15858:6714/1‑151 (MQ=60)
                                                      TCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATG                                                                                                  >  NB501016:230:HFVJLAFXY:3:11504:7564:12384/1‑151 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGA                                                                                                 <  NB501016:230:HFVJLAFXY:4:21411:7224:19774/151‑1 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATG                                                                                                  >  NB501016:230:HFVJLAFXY:1:11104:18347:8748/1‑150 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATG                                                                                                  >  NB501016:230:HFVJLAFXY:2:11203:16399:12780/1‑150 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATG                                                                                                  <  NB501016:230:HFVJLAFXY:2:21301:24638:2801/150‑1 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTGATG                                                                                                  >  NB501016:230:HFVJLAFXY:3:11407:6675:18264/1‑150 (MQ=60)
                                                       CCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACAC                                                                                                                      <  NB501016:230:HFVJLAFXY:4:21608:4192:20083/130‑1 (MQ=60)
                                                        CCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTATGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGAT                                                                                                <  NB501016:230:HFVJLAFXY:2:21101:22050:6235/151‑1 (MQ=60)
                                                          GGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTT                                                                                              >  NB501016:230:HFVJLAFXY:3:21411:9749:7650/1‑151 (MQ=60)
                                                            ATTTACTATGTCCACAGCCCTGACGGGGAACTTCTCTTCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAG                                                                                            <  NB501016:230:HFVJLAFXY:4:11606:17476:5205/151‑1 (MQ=60)
                                                            ATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGTAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAG                                                                                            <  NB501016:230:HFVJLAFXY:3:21607:19337:3331/151‑1 (MQ=60)
                                                            ATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAAAGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTT                                                                                              >  NB501016:230:HFVJLAFXY:2:11305:17811:10734/1‑149 (MQ=60)
                                                              TAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGC                                                                                           <  NB501016:230:HFVJLAFXY:1:21205:12525:18397/150‑1 (MQ=60)
                                                               AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCGAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGT                                                                                         >  NB501016:230:HFVJLAFXY:1:21107:5229:16224/1‑151 (MQ=60)
                                                               AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCACCGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGT                                                                                         >  NB501016:230:HFVJLAFXY:2:11108:8095:13714/1‑151 (MQ=60)
                                                               AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGT                                                                                         >  NB501016:230:HFVJLAFXY:4:21611:6621:16264/1‑151 (MQ=60)
                                                               AACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGT                                                                                         >  NB501016:230:HFVJLAFXY:1:21308:4030:19108/1‑151 (MQ=60)
                                                                ACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTG                                                                                        <  NB501016:230:HFVJLAFXY:3:21609:11852:14681/151‑1 (MQ=60)
                                                                ACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTG                                                                                        >  NB501016:230:HFVJLAFXY:2:21108:1802:15000/1‑151 (MQ=60)
                                                                ACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGGTTAGCGCGTA‑CCCGTATTGCATTATGCCAACGCCCCGGTGCTGCCACGGAAGACACCGGACGTTATGATTTAGCGTG                                                                                        >  NB501016:230:HFVJLAFXY:3:21611:10567:10938/1‑151 (MQ=60)
                                                                 CTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGG                                                                                       >  NB501016:230:HFVJLAFXY:2:11303:20730:19852/1‑151 (MQ=60)
                                                                 CTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACG                                                                                                       <  NB501016:230:HFVJLAFXY:3:21406:17390:4435/135‑1 (MQ=60)
                                                                  TATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGCTGCACACAGGGTGTAGCGCGTA‑CACGTATTGCATTATGCCAACTCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTTAGCGGGG                                                                                       >  NB501016:230:HFVJLAFXY:4:11502:3395:15943/1‑150 (MQ=60)
                                                                   ATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGG                                                                                       <  NB501016:230:HFVJLAFXY:4:21509:18167:1613/149‑1 (MQ=60)
                                                                    TGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:4:11508:17589:9293/1‑150 (MQ=60)
                                                                     GTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAA                                                                                    <  NB501016:230:HFVJLAFXY:4:11611:16110:5196/150‑1 (MQ=60)
                                                                     GTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:1:11311:21804:1312/1‑149 (MQ=60)
                                                                      TCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AGAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:1:21304:24192:11399/1‑148 (MQ=60)
                                                                      TCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:1:21306:13667:16544/1‑148 (MQ=60)
                                                                      TCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:3:21410:4250:10307/1‑148 (MQ=60)
                                                                      TCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAA                                                                                     >  NB501016:230:HFVJLAFXY:1:11309:15287:15359/1‑148 (MQ=60)
                                                                         ACAGCCCTGTCGGGGAACTTCTCTGGGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTTGCTGGTA‑CACGTATTGCATTATGCGAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTT                                                                               <  NB501016:230:HFVJLAFXY:3:11411:21777:13855/151‑1 (MQ=60)
                                                                         ACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTT                                                                               <  NB501016:230:HFVJLAFXY:2:11307:21979:3959/151‑1 (MQ=60)
                                                                          CAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTG                                                                              <  NB501016:230:HFVJLAFXY:3:21505:13149:20332/151‑1 (MQ=60)
                                                                          CAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTG                                                                              <  NB501016:230:HFVJLAFXY:2:11212:15779:1694/151‑1 (MQ=60)
                                                                          CAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTG                                                                              <  NB501016:230:HFVJLAFXY:3:11602:20045:2141/151‑1 (MQ=60)
                                                                          CAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTG                                                                              <  NB501016:230:HFVJLAFXY:2:21307:22908:5220/151‑1 (MQ=60)
                                                                           AGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGT                                                                             <  NB501016:230:HFVJLAFXY:2:21310:9644:9840/151‑1 (MQ=60)
                                                                           AGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGAACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGAAACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGT                                                                             <  NB501016:230:HFVJLAFXY:3:21507:18786:20351/151‑1 (MQ=60)
                                                                            GCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTG                                                                            >  NB501016:230:HFVJLAFXY:2:21309:5864:18685/1‑151 (MQ=60)
                                                                            GCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTG                                                                            >  NB501016:230:HFVJLAFXY:3:21510:7918:9618/1‑151 (MQ=60)
                                                                            GCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTG                                                                            <  NB501016:230:HFVJLAFXY:1:21210:14528:8455/151‑1 (MQ=60)
                                                                            GCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTT                                                                                              >  NB501016:230:HFVJLAFXY:1:11205:9517:20272/1‑133 (MQ=60)
                                                                            GCCCTGACGGGGAACTTCTCTGCGGGAGTGTCAGGGTATTATT‑AAAACGATGCACTCAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTG                                                                            <  NB501016:230:HFVJLAFXY:3:21502:25118:16342/151‑1 (MQ=60)
                                                                             CCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           <  NB501016:230:HFVJLAFXY:1:21203:5929:1598/151‑1 (MQ=60)
                                                                               CTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           >  NB501016:230:HFVJLAFXY:4:21407:2874:14917/1‑149 (MQ=60)
                                                                               CTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           >  NB501016:230:HFVJLAFXY:1:11307:4557:16737/1‑149 (MQ=60)
                                                                               CTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           >  NB501016:230:HFVJLAFXY:4:11603:18828:14538/1‑149 (MQ=60)
                                                                               CTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           >  NB501016:230:HFVJLAFXY:4:11409:25073:17172/1‑149 (MQ=60)
                                                                                 GACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAG                                                                         <  NB501016:230:HFVJLAFXY:4:21403:12861:12344/149‑1 (MQ=60)
                                                                                 GACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGGA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGAGTTAGCGTGGAAAGATTTGTGTAGTG                                                                       <  NB501016:230:HFVJLAFXY:3:11507:16732:10140/151‑1 (MQ=60)
                                                                                  ACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGT                                                                      <  NB501016:230:HFVJLAFXY:2:21205:11607:10384/151‑1 (MQ=60)
                                                                                   CGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTT                                                                     >  NB501016:230:HFVJLAFXY:3:11604:9441:10868/1‑151 (MQ=60)
                                                                                   CGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTT                                                                     >  NB501016:230:HFVJLAFXY:1:11203:18621:4805/1‑151 (MQ=60)
                                                                                   CGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAG                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21110:24080:7332/107‑1 (MQ=60)
                                                                                     GGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAACACGGCAGTTATGATTTAGCTTGGAAAGATTTGAGTAGTGTTCT                                                                   >  NB501016:230:HFVJLAFXY:3:11406:23388:2345/1‑151 (MQ=60)
                                                                                     GGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCT                                                                   >  NB501016:230:HFVJLAFXY:4:21404:23228:5126/1‑151 (MQ=60)
                                                                                     GGGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCT                                                                   <  NB501016:230:HFVJLAFXY:2:21112:2490:17313/151‑1 (MQ=60)
                                                                                     GGGAACTTCTCTGCGGGAGTGTCAGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCT                                                                   <  NB501016:230:HFVJLAFXY:4:21410:6916:16130/151‑1 (MQ=60)
                                                                                     GGGAACTTCTCTGCGGGAGTGGCCGGGAATAATT‑AAACCGATGCACACATGGTTTAGCGCGTA‑AACGTATTGCATTATTACAACGCCCCGGTGCTGACACTGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCT                                                                   <  NB501016:230:HFVJLAFXY:1:21112:20673:17577/151‑1 (MQ=60)
                                                                                      GGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTG                                                                  <  NB501016:230:HFVJLAFXY:4:21610:10207:20112/151‑1 (MQ=60)
                                                                                      GGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTG                                                                  <  NB501016:230:HFVJLAFXY:3:21509:13570:11689/151‑1 (MQ=60)
                                                                                      GGAACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTG                                                                  <  NB501016:230:HFVJLAFXY:3:11606:18246:2901/151‑1 (MQ=60)
                                                                                        AACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAA                                                                <  NB501016:230:HFVJLAFXY:4:21607:16263:7731/151‑1 (MQ=60)
                                                                                         ACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAT                                                               >  NB501016:230:HFVJLAFXY:2:11201:23756:18186/1‑151 (MQ=60)
                                                                                         ACTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTG                                                                            >  NB501016:230:HFVJLAFXY:1:11210:19088:15454/1‑138 (MQ=60)
                                                                                          CTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGTTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATG                                                              >  NB501016:230:HFVJLAFXY:4:11609:4756:20139/1‑151 (MQ=60)
                                                                                          CTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CCCGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATG                                                              >  NB501016:230:HFVJLAFXY:1:21104:5886:17376/1‑151 (MQ=60)
                                                                                          CTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATG                                                              >  NB501016:230:HFVJLAFXY:3:21601:18148:5830/1‑151 (MQ=60)
                                                                                          CTTCTCTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTG                                                                              >  NB501016:230:HFVJLAFXY:2:21305:13232:8924/1‑135 (MQ=60)
                                                                                            TCTCTGCGGGAGTGTCCGGGAATAATT‑AAAAGGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACAGGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATG                                                              <  NB501016:230:HFVJLAFXY:2:21312:2427:4761/149‑1 (MQ=60)
                                                                                               CTGCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTATGAATGCTCTC                                                         >  NB501016:230:HFVJLAFXY:2:11309:22750:17313/1‑151 (MQ=60)
                                                                                                 GCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAG                                                       <  NB501016:230:HFVJLAFXY:2:11209:20261:16545/151‑1 (MQ=60)
                                                                                                 GCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCA                                                        <  NB501016:230:HFVJLAFXY:2:21203:6514:10773/150‑1 (MQ=60)
                                                                                                 GCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTC                                                         >  NB501016:230:HFVJLAFXY:4:21601:19439:3740/1‑149 (MQ=60)
                                                                                                 GCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTC                                                         <  NB501016:230:HFVJLAFXY:2:11110:15155:5567/149‑1 (MQ=60)
                                                                                                 GCGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGAGTTGTGTAGTGTTCTGAATGCTCT                                                          <  NB501016:230:HFVJLAFXY:3:21405:1808:11965/148‑1 (MQ=60)
                                                                                                  CGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACATGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGTTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGT                                                      <  NB501016:230:HFVJLAFXY:3:21603:4122:1321/151‑1 (MQ=60)
                                                                                                  CGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CCCGTATTGCATTATGCCAACGCCCCGGTGCTGCCACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGT                                                      >  NB501016:230:HFVJLAFXY:2:21210:21551:7831/1‑151 (MQ=60)
                                                                                                  CGGGAGTGTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGT                                                      >  NB501016:230:HFVJLAFXY:2:11302:14637:2763/1‑151 (MQ=60)
                                                                                                         ttcctGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                <  NB501016:230:HFVJLAFXY:2:11102:16007:1182/145‑1 (MQ=60)
                                                                                                         GTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                <  NB501016:230:HFVJLAFXY:3:11404:6299:17750/150‑1 (MQ=60)
                                                                                                         GTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                <  NB501016:230:HFVJLAFXY:4:21401:6656:17164/150‑1 (MQ=60)
                                                                                                         GTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                <  NB501016:230:HFVJLAFXY:3:11409:20292:6498/150‑1 (MQ=60)
                                                                                                         GTCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAAT                                                               <  NB501016:230:HFVJLAFXY:3:11508:23392:12847/135‑1 (MQ=60)
                                                                                                          TCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGT                                               >  NB501016:230:HFVJLAFXY:2:21208:2524:8651/1‑150 (MQ=60)
                                                                                                          TCCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTG                                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11608:21759:13467/51‑1 (MQ=57)
                                                                                                           CCGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                <  NB501016:230:HFVJLAFXY:1:11201:12603:15577/148‑1 (MQ=60)
                                                                                                            CGGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGT                                                      >  NB501016:230:HFVJLAFXY:3:21612:26395:8271/1‑141 (MQ=60)
                                                                                                            CGGGAATAATT‑AAAACGATGAACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACTCCCCGCTGCTGACACGGAAGAAACCGGACGTTATCATTTAGCGTGGAAAGATTAGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAA                                             >  NB501016:230:HFVJLAFXY:2:11207:16695:10714/1‑150 (MQ=60)
                                                                                                             GGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATG                                           >  NB501016:230:HFVJLAFXY:3:11601:15688:14963/1‑151 (MQ=60)
                                                                                                             GGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATG                                           <  NB501016:230:HFVJLAFXY:1:21106:26417:8365/151‑1 (MQ=60)
                                                                                                             GGGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATG                                           >  NB501016:230:HFVJLAFXY:2:11211:20837:15774/1‑151 (MQ=60)
                                                                                                              GTAATAATT‑AAACCGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCTTTATGCCTACGCACCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAT                                            <  NB501016:230:HFVJLAFXY:2:21102:3225:12898/149‑1 (MQ=60)
                                                                                                              GGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATG                                           >  NB501016:230:HFVJLAFXY:4:21504:2788:7847/1‑150 (MQ=60)
                                                                                                              GGAATAATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATG                                           >  NB501016:230:HFVJLAFXY:1:11302:17544:5390/1‑150 (MQ=60)
                                                                                                                 ATAATT‑AAAACGATGCACACTGGG‑TTAGAGCGTT‑CAGGTATTGCTTTATGCCAACGCCCCGGTGCTGACAGGTAAGATACCGGACTTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTATATAGTAATGAATTA                                      <  NB501016:230:HFVJLAFXY:1:11303:3362:9414/151‑1 (MQ=60)
                                                                                                                    ATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATC                                    >  NB501016:230:HFVJLAFXY:2:11104:9104:19575/1‑151 (MQ=60)
                                                                                                                    ATT‑AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATAATC                                    >  NB501016:230:HFVJLAFXY:1:11301:1362:8936/1‑151 (MQ=60)
                                                                                                                        AAAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTTTGATTTAGCGGGGAAAGATTTGGGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAA                                  >  NB501016:230:HFVJLAFXY:2:21111:15818:10130/1‑150 (MQ=60)
                                                                                                                         AAACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACTCGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAG                                <  NB501016:230:HFVJLAFXY:4:21604:6898:17569/151‑1 (MQ=60)
                                                                                                                          AACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGG                               <  NB501016:230:HFVJLAFXY:1:21307:19828:6876/151‑1 (MQ=60)
                                                                                                                           ACGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGT                              >  NB501016:230:HFVJLAFXY:1:21206:13762:11824/1‑151 (MQ=60)
                                                                                                                            CGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTA                             <  NB501016:230:HFVJLAFXY:1:21207:10953:7932/151‑1 (MQ=60)
                                                                                                                            CGATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACG                                                                                                       <  NB501016:230:HFVJLAFXY:1:11112:10540:1507/77‑1 (MQ=60)
                                                                                                                             GATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTAT                            <  NB501016:230:HFVJLAFXY:1:11108:20049:12159/151‑1 (MQ=60)
                                                                                                                             GATGCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTAT                            <  NB501016:230:HFVJLAFXY:4:11607:3872:10939/151‑1 (MQ=60)
                                                                                                                                GCACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAG                          <  NB501016:230:HFVJLAFXY:4:21412:21943:1823/150‑1 (MQ=60)
                                                                                                                                GCACACAGGGATTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCTGACGTTATGATTTAGAGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGT                         <  NB501016:230:HFVJLAFXY:3:21506:12346:6297/151‑1 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGCCACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGT                         >  NB501016:230:HFVJLAFXY:3:11505:18965:2106/1‑150 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGT                         >  NB501016:230:HFVJLAFXY:2:21104:19688:16957/1‑150 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGT                         >  NB501016:230:HFVJLAFXY:4:21512:14312:8863/1‑150 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAG                                                >  NB501016:230:HFVJLAFXY:4:21606:15701:12673/1‑127 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCA                                                        <  NB501016:230:HFVJLAFXY:2:21308:25057:1323/119‑1 (MQ=60)
                                                                                                                                 CACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCT                                                            <  NB501016:230:HFVJLAFXY:3:21407:18394:17294/115‑1 (MQ=60)
                                                                                                                                  ACACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTA                        >  NB501016:230:HFVJLAFXY:2:11210:23104:11730/1‑150 (MQ=60)
                                                                                                                                   CACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAAT                      >  NB501016:230:HFVJLAFXY:2:21302:25535:10935/1‑151 (MQ=60)
                                                                                                                                   CACAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGT                                                                           >  NB501016:230:HFVJLAFXY:1:21211:1633:12125/1‑98 (MQ=60)
                                                                                                                                     CAGGGTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATAT                    >  NB501016:230:HFVJLAFXY:1:21212:25979:14643/1‑151 (MQ=60)
                                                                                                                                         GTTTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTT                >  NB501016:230:HFVJLAFXY:1:21303:14774:14542/1‑151 (MQ=60)
                                                                                                                                           TTAGCGCGTA‑CACGTATTTCATTATGCCAACGCCCCGGTGCTGACACTGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTT               <  NB501016:230:HFVJLAFXY:1:11105:5595:18094/150‑1 (MQ=60)
                                                                                                                                           TTAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTTCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTT               <  NB501016:230:HFVJLAFXY:1:21202:24502:8718/150‑1 (MQ=60)
                                                                                                                                            TAGCGCGTAGCAT‑TATTGCATTATGCCTACGCCGCTGTGCTGACACGGAAGTAACCGGACGTTATGATTTAGCGTGGATAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTA              <  NB501016:230:HFVJLAFXY:3:11510:22469:9575/150‑1 (MQ=60)
                                                                                                                                            TAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTATCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTA              <  NB501016:230:HFVJLAFXY:3:11603:12668:15071/150‑1 (MQ=60)
                                                                                                                                            TAGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTA              <  NB501016:230:HFVJLAFXY:3:21404:1058:8468/150‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTTCATTATGACAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAT             <  NB501016:230:HFVJLAFXY:3:11412:9766:14710/150‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATG            <  NB501016:230:HFVJLAFXY:4:11509:22221:11168/151‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATG            <  NB501016:230:HFVJLAFXY:4:11612:22653:11527/151‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATG            <  NB501016:230:HFVJLAFXY:4:21604:15862:7064/151‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAT             <  NB501016:230:HFVJLAFXY:4:21505:23162:8620/150‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAT             <  NB501016:230:HFVJLAFXY:2:11312:12255:14090/150‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAT             <  NB501016:230:HFVJLAFXY:4:11412:9722:11531/150‑1 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATC                                    >  NB501016:230:HFVJLAFXY:2:21212:10663:16662/1‑127 (MQ=60)
                                                                                                                                             AGCGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTATGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCA                                   >  NB501016:230:HFVJLAFXY:1:11107:12459:14040/1‑128 (MQ=60)
                                                                                                                                               CGCGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTT          >  NB501016:230:HFVJLAFXY:2:11204:6059:16833/1‑151 (MQ=60)
                                                                                                                                               CGCGTA‑CACGTATTGCATTATGCCAACGCACCTGTGCTGAAACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTT          <  NB501016:230:HFVJLAFXY:2:21202:19608:1265/151‑1 (MQ=60)
                                                                                                                                                 CGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTC         >  NB501016:230:HFVJLAFXY:4:11407:11531:19139/1‑150 (MQ=60)
                                                                                                                                                 CGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTC         >  NB501016:230:HFVJLAFXY:4:11502:11831:2476/1‑150 (MQ=60)
                                                                                                                                                 CGTA‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTT               >  NB501016:230:HFVJLAFXY:2:21207:21533:12693/1‑144 (MQ=60)
                                                                                                                                                    A‑CACGTATTGCATTATTCGAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATG      <  NB501016:230:HFVJLAFXY:4:21507:26366:18696/150‑1 (MQ=60)
                                                                                                                                                    A‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGG     <  NB501016:230:HFVJLAFXY:4:11402:5361:11033/151‑1 (MQ=60)
                                                                                                                                                    A‑CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATG      <  NB501016:230:HFVJLAFXY:1:11207:4458:14198/150‑1 (MQ=60)
                                                                                                                                                      CACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTA                                      >  NB501016:230:HFVJLAFXY:4:11411:3439:11556/1‑117 (MQ=60)
                                                                                                                                                       ACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTATTGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGAT   >  NB501016:230:HFVJLAFXY:2:21109:26082:16640/1‑151 (MQ=60)
                                                                                                                                                        CGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGG     <  NB501016:230:HFVJLAFXY:4:11408:16929:11940/148‑1 (MQ=60)
                                                                                                                                                        CGTATTGCATTATGCCAAAGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCTAAGGTAGAGTAATATCTTTTATGTTgatggatt  >  NB501016:230:HFVJLAFXY:1:21309:25692:7488/1‑143 (MQ=60)
                                                                                                                                                        |                                                                                                                                                      
TCATATTGTTAATATTTATTAATGTATGCCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGGGGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAAACGATGCACACAGGGTTTAGCGCGTA‑CATGTATTGTATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATA  >  NC_000913/578976‑579277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑