Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 579,285 T→C 100% I104V (ATT→GTT)  ybcV ← DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,2850TC98.6% 13223.5 / 49.9 4682I104V (ATT→GTT) ybcVDLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base C (2543/2044);  total (2596/2086)
Fisher's exact test for biased strand distribution p-value = 9.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-02
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 4685 total aligned reads displayed.

TGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT  >  NC_000913/579139‑579430
                                                                                                                                                  |                                                                                                                                                 
 gCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCa                                                                                                                                                >  6:39929/1‑149 (MQ=255)
    aaCGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCg                                                                                                                                             >  2:29421/1‑149 (MQ=255)
    aaCGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCg                                                                                                                                             >  6:270024/1‑149 (MQ=255)
     acgccccGGTGCTGACACGGAAGCAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGAAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTGATGTTCATGGATATTTGTAGCCCATCg                                                                                                                                             >  6:295732/4‑148 (MQ=18)
       gccccGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGa                                                                                                                                           >  1:183741/2‑148 (MQ=255)
         cccGTTGCTGAGACTGAAGCAACCGGACGTAATTATTTAGCGTGGAAAGATTTTTGTACTGTTATGAATGCTCTCAGTAAATCGTAATTAATTATCAAAGGTATATTAATATCTTTTATTTTCCTTGATATTTGTAACACATCTTaaaa                                                                                                                                        <  6:10693/149‑1 (MQ=255)
         cccGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGaaaa                                                                                                                                        >  6:97578/1‑149 (MQ=255)
            ggTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTc                                                                                                                                     <  1:266911/149‑1 (MQ=255)
            ggTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGATCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTc                                                                                                                                     <  7:309940/149‑1 (MQ=255)
             gTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTcc                                                                                                                                    <  3:351654/149‑1 (MQ=255)
              tGCTGACACGGAAGAAACCGCACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCt                                                                                                                                   <  3:71968/149‑1 (MQ=255)
                   acacGTAAGAAACCGGACGTTAAGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATTAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGc                                                                                                                                 <  3:245456/146‑1 (MQ=17)
                   acacGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGc                                                                                                                                 >  8:173676/1‑146 (MQ=255)
                      cGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGc                                                                                                                           <  3:129877/149‑1 (MQ=255)
                          agaaACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGa                                                                                                                       <  1:215135/149‑1 (MQ=255)
                              aCCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGAtttt                                                                                                                   >  8:119904/1‑149 (MQ=255)
                               ccGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTc                                                                                                                  >  8:70589/1‑149 (MQ=255)
                                cGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGaaaa                                                                                                                                        <  7:20030/126‑1 (MQ=255)
                                 ggACGTTATGATATAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGATCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACACATCGGAAAACTCCTGCTTTAGCAAGATTTTccc                                                                                                                >  5:141807/1‑149 (MQ=17)
                                       tATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTc                                                                                                                                     <  2:155754/122‑1 (MQ=255)
                                       tATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCt                                                                                                               <  4:156318/144‑1 (MQ=255)
                                        attttttAGCGTTGAATGATTTGTGTTGTGTTCTGGATGCTCTCAGTAAATAGTACTGAATTATCAAATGTATAGTAATATCTTTTATGTGCATGGATATTTGTATCGCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTAtt                                                                                                          <  1:100768/144‑1 (MQ=255)
                                          gATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCt                                                                                                       >  8:225243/1‑149 (MQ=255)
                                          gATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCACTGTATTGCt                                                                                                       >  3:305360/1‑149 (MQ=255)
                                          gATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTATAGCAAGATTTTCCCTGTATTGCt                                                                                                       >  4:219182/1‑149 (MQ=255)
                                           aTTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCt                                                                                                       >  2:235695/1‑148 (MQ=255)
                                           aTTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAAAGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTg                                                                                                      <  3:189629/149‑1 (MQ=255)
                                                gCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAtg                                                                                                 >  7:172768/1‑149 (MQ=255)
                                                  gTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAtgt                                                                                                >  6:220910/1‑148 (MQ=255)
                                                      aaaGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGAttt                                                                                           <  1:372971/149‑1 (MQ=255)
                                                          aTTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTctct                                                                                       >  6:7155/1‑149 (MQ=255)
                                                          aTTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTctct                                                                                       >  1:131675/1‑149 (MQ=255)
                                                           tttGTGTAGTGGTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCtt                                                                                      <  4:125822/149‑1 (MQ=255)
                                                            ttGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCtt                                                                                      >  4:354743/1‑148 (MQ=255)
                                                              gtgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGa                                                                                    <  7:257589/148‑1 (MQ=255)
                                                              gtgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTCTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGAt                                                                                   >  5:312277/1‑149 (MQ=255)
                                                               tgtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGAtt                                                                                  >  4:326592/1‑149 (MQ=255)
                                                                gtAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGAttt                                                                                 >  4:248366/1‑149 (MQ=255)
                                                                   gtgtTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCaa                                                                              <  3:219307/149‑1 (MQ=255)
                                                                    tgtTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGc                                                                                                        >  3:325669/1‑122 (MQ=255)
                                                                    tgtTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAc                                                                             <  6:35662/149‑1 (MQ=255)
                                                                     gtTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAcc                                                                            >  8:343034/1‑149 (MQ=255)
                                                                      ttCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAcc                                                                            >  3:104914/1‑148 (MQ=255)
                                                                         tGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCt                                                                                                               >  4:54102/1‑110 (MQ=255)
                                                                         tGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTAt                                                                         >  5:376399/1‑148 (MQ=255)
                                                                          gAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAtg                                                                                                 >  6:164863/1‑123 (MQ=255)
                                                                           aaTGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATc                                                                        <  1:341939/147‑1 (MQ=255)
                                                                            aTGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATa                                                                     <  6:13276/149‑1 (MQ=255)
                                                                               ctctCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGa                                                                  <  5:57353/149‑1 (MQ=255)
                                                                                tctcAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGAc                                                                 >  4:302180/1‑149 (MQ=255)
                                                                                tctcAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGAc                                                                 >  5:168443/1‑149 (MQ=255)
                                                                                  tcAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGt                                                               >  5:84486/1‑149 (MQ=255)
                                                                                  tcAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGt                                                               >  8:281944/1‑149 (MQ=255)
                                                                                    aGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGttt                                                             >  2:371572/1‑149 (MQ=255)
                                                                                        aaTAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCta                                                          <  6:196794/148‑1 (MQ=255)
                                                                                         aTAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCtat                                                         <  8:370389/148‑1 (MQ=255)
                                                                                          tAGTAATGAATTATCAAAGGTATAGTAATATATTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATaa                                                       <  7:282847/149‑1 (MQ=255)
                                                                                            gTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCtat                                                         >  5:228894/1‑145 (MQ=255)
                                                                                            gTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAg                                                      <  7:225192/148‑1 (MQ=255)
                                                                                             tAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAc                                                                             >  7:145351/1‑124 (MQ=255)
                                                                                             tAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAg                                                      >  1:153490/1‑147 (MQ=255)
                                                                                               aTGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGc                                                  <  4:278590/149‑1 (MQ=255)
                                                                                               aTGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGc                                                  >  8:146994/1‑149 (MQ=255)
                                                                                                tGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCg                                                 >  7:117807/1‑149 (MQ=255)
                                                                                                 gAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGt                                                >  3:160490/1‑149 (MQ=255)
                                                                                                 gAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGt                                                >  2:178363/1‑149 (MQ=255)
                                                                                                  aaTTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGt                                                <  7:60477/148‑1 (MQ=255)
                                                                                                    ttATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTa                                                                                                            <  4:189386/86‑1 (MQ=255)
                                                                                                      aTCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGttt                                            <  2:324216/148‑1 (MQ=255)
                                                                                                      aTCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGttt                                            <  7:391596/148‑1 (MQ=255)
                                                                                                       tCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTc                                           <  5:251626/148‑1 (MQ=255)
                                                                                                       tCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCt                                          <  7:343034/149‑1 (MQ=255)
                                                                                                         aaaGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCtt                                         >  1:322717/1‑148 (MQ=255)
                                                                                                         aaaGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCtt                                         >  3:282937/1‑148 (MQ=255)
                                                                                                         aaaGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTg                                        <  3:43111/149‑1 (MQ=255)
                                                                                                            ggTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTgag                                      >  2:69672/1‑148 (MQ=255)
                                                                                                              tataGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAAt                                   <  5:204532/149‑1 (MQ=255)
                                                                                                                  gTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGtg                                               >  2:105671/1‑132 (MQ=255)
                                                                                                                  gTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTaa                               <  2:397236/149‑1 (MQ=255)
                                                                                                                   tAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATaa                                                       >  2:264829/1‑124 (MQ=255)
                                                                                                                   tAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTga                                       >  2:209830/1‑140 (MQ=255)
                                                                                                                   tAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTaa                               >  5:112828/1‑148 (MQ=255)
                                                                                                                     atatCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCACTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACa                             <  1:41/148‑1 (MQ=255)
                                                                                                                      tatCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACAtt                           >  8:258053/1‑149 (MQ=255)
                                                                                                                       atCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACAtt                           >  6:330728/1‑148 (MQ=255)
                                                                                                                          ttttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTAc                        >  1:296235/1‑148 (MQ=255)
                                                                                                                            ttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGTGTGTTTCTTGAGAATTTAACATTTACaa                      <  1:74527/148‑1 (MQ=255)
                                                                                                                            ttATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTAc                        <  7:91574/146‑1 (MQ=255)
                                                                                                                               tGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCtt                  <  5:351290/149‑1 (MQ=255)
                                                                                                                                   catGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACACCCttttt               >  4:79779/3‑148 (MQ=255)
                                                                                                                                   catGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCttttt               <  7:369972/146‑1 (MQ=255)
                                                                                                                                    atGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTaa             <  8:203829/148‑1 (MQ=255)
                                                                                                                                    atGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTaa             <  1:244448/148‑1 (MQ=255)
                                                                                                                                    atGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGAGTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTAAAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCttatt               >  2:133376/2‑147 (MQ=255)
                                                                                                                                        atatTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTcc         >  8:314545/1‑149 (MQ=255)
                                                                                                                                           tttGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCttt      <  5:280089/149‑1 (MQ=255)
                                                                                                                                             tGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCtttt     <  8:398322/148‑1 (MQ=255)
                                                                                                                                             tGTAACCCATCATAAAACTCCTGCTTTAGCAAGATTTTCGCTGTTTTGCTGAAATGTGATTTCTATTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCtttt     <  2:343849/148‑1 (MQ=37)
                                                                                                                                              gTAACCCATCGGAAACCTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTtta    <  4:100021/148‑1 (MQ=255)
                                                                                                                                              gTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTtta    <  6:243310/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT  >  NC_000913/579139‑579430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 2527 total aligned reads displayed.
ATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACG  >  NC_000913/579135‑579436
                                                                                                                                                      |                                                                                                                                                       
  TATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACC                                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11302:16412:3591/150‑1 (MQ=60)
      CCAACGCCCCTGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAATATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCG                                                                                                                                                   <  NB501016:230:HFVJLAFXY:1:21109:5571:5071/151‑1 (MQ=60)
      CCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:2:11109:1209:8180/1‑151 (MQ=60)
      CCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:1:21202:3903:12692/1‑151 (MQ=60)
      CCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:3:11611:5517:9736/1‑151 (MQ=60)
      CCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCACTAAATAGTAATGAATTAGCAAAGGTATAGTAATATCTGTTATGTTCATGGATAGTTGTAACCCATCG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:3:11508:1131:4068/1‑151 (MQ=60)
       CAACGCCCCGGTGCTGACACGGAAGAACCCGGACGTTATGATTAAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCATTTATCTTCATGGATATTTGTAACCCATCGG                                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21309:4953:11546/1‑151 (MQ=60)
       CAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGG                                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:11312:10678:13139/1‑151 (MQ=60)
       CAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGG                                                                                                                                                  >  NB501016:230:HFVJLAFXY:3:21610:10241:14861/1‑151 (MQ=60)
        AACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTTGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGA                                                                                                                                                 <  NB501016:230:HFVJLAFXY:2:21102:9452:17750/151‑1 (MQ=60)
        AACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGG                                                                                                                                                  >  NB501016:230:HFVJLAFXY:4:11603:22544:5613/1‑150 (MQ=60)
            CCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAAC                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11301:20433:3790/1‑151 (MQ=60)
              CCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTC                                                                                                                                           >  NB501016:230:HFVJLAFXY:3:21604:15680:19595/1‑151 (MQ=60)
                 GTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:11209:26499:5676/151‑1 (MQ=60)
                 GTGCTGACACGGAAGAAACCGCACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:11203:17797:7986/151‑1 (MQ=60)
                     TGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTT                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21104:20458:1918/1‑151 (MQ=60)
                      GACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTT                                                                                                                                   >  NB501016:230:HFVJLAFXY:4:21606:11891:4262/1‑151 (MQ=60)
                      GACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTA                                                                                                                                   <  NB501016:230:HFVJLAFXY:1:11111:8612:5313/151‑1 (MQ=60)
                      GACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTT                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21303:26175:11120/1‑150 (MQ=60)
                      GACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCT                                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11607:4182:3290/148‑1 (MQ=60)
                       ACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTTTTCTGAATGCTCTCATTAAATAGTAATGAATTATCAAAGGTATATTAATATCTTTTTTGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:21609:1780:5986/151‑1 (MQ=59)
                         ACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCA                                                                                                                                <  NB501016:230:HFVJLAFXY:2:21201:16197:3135/151‑1 (MQ=60)
                          CGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTG                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21209:14316:19062/142‑1 (MQ=60)
                           GGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCA                                                                                                                                >  NB501016:230:HFVJLAFXY:4:11503:23136:12876/1‑149 (MQ=60)
                            GAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCA                                                                                                                                >  NB501016:230:HFVJLAFXY:2:11312:1344:20116/1‑148 (MQ=60)
                             AAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCA                                                                                                                                >  NB501016:230:HFVJLAFXY:3:21607:16982:11949/1‑147 (MQ=60)
                              AGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATT                                                                                                                           >  NB501016:230:HFVJLAFXY:4:11404:20967:17750/1‑151 (MQ=60)
                                 AACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTC                                                                                                                        >  NB501016:230:HFVJLAFXY:3:11607:12451:16791/1‑151 (MQ=60)
                                  ACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCC                                                                                                                       <  NB501016:230:HFVJLAFXY:2:21207:5797:5487/151‑1 (MQ=60)
                                   CCGGACGTTATGTTTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCC                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11102:9170:3197/151‑1 (MQ=60)
                                   CCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCC                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11206:12922:13766/151‑1 (MQ=60)
                                    CGGACGTTATGATATAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGATCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACACATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCT                                                                                                                     >  NB501016:230:HFVJLAFXY:3:11603:20431:14654/1‑151 (MQ=60)
                                     GGACGTTATGATTTATCTTGGAAATATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTG                                                                                                                    <  NB501016:230:HFVJLAFXY:3:21505:20307:2288/151‑1 (MQ=60)
                                     GGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTG                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21401:24808:8129/1‑151 (MQ=60)
                                      gactggagttcagaccgtgtgctcttccgatctGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTG                                                                                                                    <  NB501016:230:HFVJLAFXY:1:21307:14334:7685/117‑1 (MQ=60)
                                        CGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTAT                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11605:4397:4110/151‑1 (MQ=60)
                                          TTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTG                                                                                                               >  NB501016:230:HFVJLAFXY:1:11203:13651:13434/1‑151 (MQ=60)
                                           TATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGC                                                                                                              >  NB501016:230:HFVJLAFXY:4:11608:5470:19742/1‑151 (MQ=60)
                                            ATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCAATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCT                                                                                                             >  NB501016:230:HFVJLAFXY:3:11409:12593:19112/1‑151 (MQ=60)
                                             TGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTG                                                                                                            >  NB501016:230:HFVJLAFXY:1:21108:25378:13537/1‑151 (MQ=60)
                                                 cagatcTGGAAAGATTTGTGTAGTGTTCTTAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAT                                                                                                        <  NB501016:230:HFVJLAFXY:1:21106:5701:3932/145‑1 (MQ=60)
                                                 TTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAT                                                                                                        >  NB501016:230:HFVJLAFXY:2:21111:25580:11229/1‑151 (MQ=60)
                                                  gttgtgctcttccgatcTGTAGTGTTCTGTATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATG                                                                                                       <  NB501016:230:HFVJLAFXY:3:21403:23857:18260/134‑1 (MQ=60)
                                                     CGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGA                                                                                                    <  NB501016:230:HFVJLAFXY:1:11309:8820:19326/151‑1 (MQ=60)
                                                     CGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTG                                                                                                     <  NB501016:230:HFVJLAFXY:3:21405:5740:19591/150‑1 (MQ=60)
                                                      GTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGAT                                                                                                   >  NB501016:230:HFVJLAFXY:4:21407:6210:7310/1‑151 (MQ=60)
                                                      GTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGAT                                                                                                   >  NB501016:230:HFVJLAFXY:1:11304:3877:5662/1‑151 (MQ=60)
                                                       TGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATT                                                                                                  >  NB501016:230:HFVJLAFXY:3:21506:9014:11722/1‑151 (MQ=60)
                                                        GGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATT                                                                                                  <  NB501016:230:HFVJLAFXY:3:21508:7210:14337/150‑1 (MQ=60)
                                                        GGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATT                                                                                                                           <  NB501016:230:HFVJLAFXY:4:21511:9330:16447/125‑1 (MQ=60)
                                                        GGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCCCTGTATTGCTGAAATGTGATTT                                                                                                 >  NB501016:230:HFVJLAFXY:4:11601:14568:8912/1‑151 (MQ=60)
                                                         GAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTC                                                                                                <  NB501016:230:HFVJLAFXY:2:21302:17915:5372/151‑1 (MQ=60)
                                                         GAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTC                                                                                                <  NB501016:230:HFVJLAFXY:1:21308:22102:10531/151‑1 (MQ=60)
                                                             GATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTT                                                                                            <  NB501016:230:HFVJLAFXY:4:11606:8072:18070/151‑1 (MQ=60)
                                                             GATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTT                                                                                            <  NB501016:230:HFVJLAFXY:1:11110:3243:17715/151‑1 (MQ=60)
                                                              ATTTGTGTAGTGTTCTTAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTG                                                                                           <  NB501016:230:HFVJLAFXY:4:11408:8333:16960/151‑1 (MQ=60)
                                                               TTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATTGATATTTGTAACCCATAGGAAAACTCATGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTG                                                                                           >  NB501016:230:HFVJLAFXY:3:11512:8084:1724/1‑150 (MQ=60)
                                                               TTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCATG                                                                                                                    >  NB501016:230:HFVJLAFXY:2:21101:3136:13871/1‑125 (MQ=60)
                                                                 tgtgctattcagatatttCTCTCAGTAAATATTAATTAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGAT                                                                                         <  NB501016:230:HFVJLAFXY:3:11405:3255:16721/132‑1 (MQ=60)
                                                                  GTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTT                                                                                       >  NB501016:230:HFVJLAFXY:3:11610:18955:16820/1‑151 (MQ=60)
                                                                   tgttgttttgTGAATGATCTCAGTAAATATTAATGAATTATCAAATGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTACTTCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTT                                                                                       <  NB501016:230:HFVJLAFXY:3:11505:18506:19507/140‑1 (MQ=60)
                                                                   TGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTC                                                                                      >  NB501016:230:HFVJLAFXY:1:21305:17077:17286/1‑151 (MQ=60)
                                                                    GTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCA                                                                                     <  NB501016:230:HFVJLAFXY:3:11509:20675:8874/151‑1 (MQ=60)
                                                                    GTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCA                                                                                     <  NB501016:230:HFVJLAFXY:4:11502:15421:7357/151‑1 (MQ=60)
                                                                    GTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTC                                                                                      >  NB501016:230:HFVJLAFXY:3:11511:13164:12957/1‑150 (MQ=60)
                                                                     TAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCA                                                                                     >  NB501016:230:HFVJLAFXY:1:21110:24756:8663/1‑150 (MQ=60)
                                                                     TAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGT                                                                                                                   <  NB501016:230:HFVJLAFXY:4:11612:15871:7234/120‑1 (MQ=60)
                                                                      AGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAC                                                                                   <  NB501016:230:HFVJLAFXY:3:21407:22444:12315/151‑1 (MQ=60)
                                                                      AGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAC                                                                                   <  NB501016:230:HFVJLAFXY:4:21508:20549:1635/151‑1 (MQ=60)
                                                                      AGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAA                                                                                    <  NB501016:230:HFVJLAFXY:3:21611:9535:8887/150‑1 (MQ=60)
                                                                      AGTGTGCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAAC                                                                                   <  NB501016:230:HFVJLAFXY:1:21212:9304:15929/151‑1 (MQ=60)
                                                                       GTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACC                                                                                  <  NB501016:230:HFVJLAFXY:2:11208:10052:16344/151‑1 (MQ=60)
                                                                         GTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAAT                                                                                                        <  NB501016:230:HFVJLAFXY:4:21510:23659:14061/127‑1 (MQ=60)
                                                                           TCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATC                                                                              >  NB501016:230:HFVJLAFXY:2:21108:3010:11305/1‑151 (MQ=60)
                                                                            CTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATC                                                                              >  NB501016:230:HFVJLAFXY:4:11506:21139:3864/1‑150 (MQ=60)
                                                                            CTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGT                                                                                                      <  NB501016:230:HFVJLAFXY:4:21608:23302:16336/126‑1 (MQ=60)
                                                                              GAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATTTTTGTAACCCATCGGTAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGAT                                                                                                   <  NB501016:230:HFVJLAFXY:2:11310:24871:17333/127‑1 (MQ=60)
                                                                              GAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCAT                                                                            >  NB501016:230:HFVJLAFXY:4:11411:18134:9769/1‑150 (MQ=60)
                                                                              GAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCAT                                                                            >  NB501016:230:HFVJLAFXY:1:21210:26769:15543/1‑150 (MQ=60)
                                                                              GAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCAT                                                                            >  NB501016:230:HFVJLAFXY:2:11306:2775:8960/1‑150 (MQ=60)
                                                                              GAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCA                                                                             <  NB501016:230:HFVJLAFXY:1:11204:23843:7348/149‑1 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGAGG                                                                      >  NB501016:230:HFVJLAFXY:2:11101:19112:14427/1‑151 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACG                                                                      >  NB501016:230:HFVJLAFXY:4:21610:4622:13580/1‑151 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACG                                                                      >  NB501016:230:HFVJLAFXY:2:11307:7065:14016/1‑151 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACG                                                                      >  NB501016:230:HFVJLAFXY:4:21504:9851:10030/1‑151 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACG                                                                      >  NB501016:230:HFVJLAFXY:3:21510:13847:7510/1‑151 (MQ=60)
                                                                                   CTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACG                                                                      >  NB501016:230:HFVJLAFXY:2:11104:9606:7109/1‑151 (MQ=60)
                                                                                     CTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTT                                                                    >  NB501016:230:HFVJLAFXY:4:11509:3351:15412/1‑151 (MQ=60)
                                                                                     CTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTT                                                                    >  NB501016:230:HFVJLAFXY:4:21409:10564:12296/1‑151 (MQ=60)
                                                                                     CTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTT                                                                    >  NB501016:230:HFVJLAFXY:4:11412:6537:15990/1‑151 (MQ=60)
                                                                                     CTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTAGGTTCATGGATATTTGTAACCCATCGGAAAAAACCTGATTTAGCAAGATTTTCCCTGTAGTGCTTAAATGTGATTTCTCTTGATTTCAtcacatccatgacctcc                                                                    >  NB501016:230:HFVJLAFXY:2:21203:8664:2980/1‑134 (MQ=60)
                                                                                      TCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGTATATTTGTAACCCATCGGAAAACTCATGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTT                                                                   >  NB501016:230:HFVJLAFXY:3:11501:16386:17064/1‑151 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTC                                                                  <  NB501016:230:HFVJLAFXY:4:11511:4641:9218/151‑1 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTC                                                                  >  NB501016:230:HFVJLAFXY:2:21303:7214:9127/1‑151 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTC                                                                  <  NB501016:230:HFVJLAFXY:3:21511:8728:8900/151‑1 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTT                                                                   <  NB501016:230:HFVJLAFXY:3:21605:23615:5588/150‑1 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTT                                                                    <  NB501016:230:HFVJLAFXY:1:11307:4725:4653/149‑1 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTT                                                                    <  NB501016:230:HFVJLAFXY:2:11107:1441:1600/149‑1 (MQ=60)
                                                                                       CAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCATGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTC                                                                  >  NB501016:230:HFVJLAFXY:1:21105:19923:19087/1‑151 (MQ=60)
                                                                                        AGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTAATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTTAAATGTGAATTCTCTAGATTTCAACCTATCATAGGACGTTACT                                                                 >  NB501016:230:HFVJLAFXY:1:11208:3947:18653/1‑151 (MQ=60)
                                                                                         GTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTT                                                                                       <  NB501016:230:HFVJLAFXY:3:11403:25617:10489/128‑1 (MQ=60)
                                                                                         GTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCACTGTATTGCTGAAATGTGATTTCTCTAGATTTCAACCTATCATAGGACGTTTAT                                                                 >  NB501016:230:HFVJLAFXY:1:11109:18181:11793/1‑150 (MQ=60)
                                                                                           AAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTAT                                                               >  NB501016:230:HFVJLAFXY:4:21410:17108:2638/1‑150 (MQ=60)
                                                                                           AAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTAT                                                               >  NB501016:230:HFVJLAFXY:2:21308:16738:5442/1‑150 (MQ=60)
                                                                                            AATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAA                                                             <  NB501016:230:HFVJLAFXY:1:11207:16285:6366/151‑1 (MQ=60)
                                                                                               AGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGAT                                                          <  NB501016:230:HFVJLAFXY:3:21404:8207:12217/151‑1 (MQ=60)
                                                                                               AGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGAT                                                          <  NB501016:230:HFVJLAFXY:4:21402:7084:14297/151‑1 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATG                                                         <  NB501016:230:HFVJLAFXY:3:21409:2982:19744/151‑1 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATG                                                         >  NB501016:230:HFVJLAFXY:2:21210:3861:20060/1‑151 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATG                                                         >  NB501016:230:HFVJLAFXY:4:11604:16278:1750/1‑151 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATG                                                         >  NB501016:230:HFVJLAFXY:2:11309:18588:17225/1‑151 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATG                                                                                                       >  NB501016:230:HFVJLAFXY:2:21310:8786:15020/1‑105 (MQ=60)
                                                                                                GTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTA                                                                                                                                   <  NB501016:230:HFVJLAFXY:4:11610:25481:14153/77‑1 (MQ=26)
                                                                                                 TAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGC                                                        >  NB501016:230:HFVJLAFXY:4:21604:8651:3825/1‑151 (MQ=60)
                                                                                                 TAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGC                                                        >  NB501016:230:HFVJLAFXY:1:21102:7838:1753/1‑151 (MQ=60)
                                                                                                 TAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGC                                                        >  NB501016:230:HFVJLAFXY:2:11212:21640:4258/1‑151 (MQ=60)
                                                                                                  AATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCG                                                       <  NB501016:230:HFVJLAFXY:4:11410:11532:18982/151‑1 (MQ=60)
                                                                                                  AATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCG                                                       <  NB501016:230:HFVJLAFXY:2:21303:11787:8885/151‑1 (MQ=60)
                                                                                                  AATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCG                                                       <  NB501016:230:HFVJLAFXY:1:11105:7200:10334/151‑1 (MQ=60)
                                                                                                   ATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGT                                                      >  NB501016:230:HFVJLAFXY:4:11407:1740:1652/1‑151 (MQ=60)
                                                                                                    TGAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAAC                                                                                                                                             <  NB501016:230:HFVJLAFXY:2:11206:14256:10947/63‑1 (MQ=25)
                                                                                                     GAATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACCCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTG                                                     <  NB501016:230:HFVJLAFXY:1:21302:20679:8445/150‑1 (MQ=60)
                                                                                                       ATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAA                                                             >  NB501016:230:HFVJLAFXY:1:11104:21191:15764/1‑140 (MQ=60)
                                                                                                       ATTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGAAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTAAACCTATCATAGGACGTTTCTATAAGATGCGTGTTT                                                  >  NB501016:230:HFVJLAFXY:1:21101:14179:14470/1‑151 (MQ=60)
                                                                                                        TTATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCACTGTATTGCTGAAATGTGATTTCTCTTGATT                                                                                        >  NB501016:230:HFVJLAFXY:2:21309:26128:10153/1‑112 (MQ=60)
                                                                                                         TATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGATATGTGATTTCTCTTGATTTCAACCTATCATATGACGTTTCTATAAGATGCGTGTTTC                                                 <  NB501016:230:HFVJLAFXY:2:21301:18894:4221/150‑1 (MQ=60)
                                                                                                          ATCAAATGTATAGTAATATCTTTTATGTTCATGGATTTTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCACTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCT                                                <  NB501016:230:HFVJLAFXY:3:11411:2988:17952/150‑1 (MQ=60)
                                                                                                          ATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCTTCGTAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCT                                                <  NB501016:230:HFVJLAFXY:2:21212:12503:4952/150‑1 (MQ=60)
                                                                                                          ATCAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTG                                                                                           <  NB501016:230:HFVJLAFXY:1:11107:21242:4750/107‑1 (MQ=60)
                                                                                                            CAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTG                                              >  NB501016:230:HFVJLAFXY:1:11101:12291:7737/1‑150 (MQ=60)
                                                                                                            CAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTG                                              <  NB501016:230:HFVJLAFXY:2:11211:14827:8817/150‑1 (MQ=60)
                                                                                                             AAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAG                                            <  NB501016:230:HFVJLAFXY:3:11401:16153:3462/151‑1 (MQ=60)
                                                                                                             AAAGGTATAGTAATATCTTTAATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGAATTGCTGAAATGGGATTTCTCTTGATTTCACCCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAG                                            <  NB501016:230:HFVJLAFXY:2:11202:18659:6257/151‑1 (MQ=60)
                                                                                                                GGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAAT                                         <  NB501016:230:HFVJLAFXY:3:11606:10754:13434/151‑1 (MQ=60)
                                                                                                                GGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAAT                                         >  NB501016:230:HFVJLAFXY:1:21201:20212:12869/1‑151 (MQ=60)
                                                                                                                 GTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATT                                        <  NB501016:230:HFVJLAFXY:1:11301:17708:8086/151‑1 (MQ=60)
                                                                                                                   ATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAATCTCCTGCTTTAGCAAGATTTTCACTGTATTGCTGAAATGTGATTTCTCTTGATTTCAGCCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTGGAGAATTT                                       >  NB501016:230:HFVJLAFXY:3:11402:25219:3751/1‑150 (MQ=60)
                                                                                                                   ATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTA                                      <  NB501016:230:HFVJLAFXY:2:11110:24383:14264/151‑1 (MQ=60)
                                                                                                                     AGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    <  NB501016:230:HFVJLAFXY:2:21104:4840:19762/151‑1 (MQ=60)
                                                                                                                     AGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    <  NB501016:230:HFVJLAFXY:4:11508:18999:3061/151‑1 (MQ=60)
                                                                                                                     AGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    >  NB501016:230:HFVJLAFXY:3:21408:24687:8581/1‑151 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    >  NB501016:230:HFVJLAFXY:2:21110:20127:13729/1‑150 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTA                                      <  NB501016:230:HFVJLAFXY:1:21204:6346:9467/148‑1 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTG                                              >  NB501016:230:HFVJLAFXY:4:21507:14499:15929/1‑140 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTC                                                 >  NB501016:230:HFVJLAFXY:2:11304:3184:1270/1‑137 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCG                                                       <  NB501016:230:HFVJLAFXY:1:11212:19781:7426/131‑1 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAG                                                            <  NB501016:230:HFVJLAFXY:1:21112:13622:3533/126‑1 (MQ=60)
                                                                                                                      GTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATAATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    >  NB501016:230:HFVJLAFXY:4:11507:20736:19545/1‑150 (MQ=60)
                                                                                                                        AATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATT                                 >  NB501016:230:HFVJLAFXY:2:11112:12800:14415/1‑151 (MQ=60)
                                                                                                                         ATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTT                                <  NB501016:230:HFVJLAFXY:4:21411:18373:8932/151‑1 (MQ=60)
                                                                                                                          TATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAAT                                         <  NB501016:230:HFVJLAFXY:1:21312:13303:14302/141‑1 (MQ=60)
                                                                                                                          TATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATA                                                                           >  NB501016:230:HFVJLAFXY:3:11604:25975:3429/1‑107 (MQ=60)
                                                                                                                           gagttcagacgtgtgctccttccgatcTCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTAC                              <  NB501016:230:HFVJLAFXY:4:11402:23211:3747/124‑1 (MQ=60)
                                                                                                                           ATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCC                                                                                                                      <  NB501016:230:HFVJLAFXY:3:21504:24684:16556/63‑1 (MQ=50)
                                                                                                                            TCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCA                                                                                     <  NB501016:230:HFVJLAFXY:2:11204:24342:19189/95‑1 (MQ=60)
                                                                                                                             CTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACA                             >  NB501016:230:HFVJLAFXY:4:21502:15793:17653/1‑150 (MQ=60)
                                                                                                                              TTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAAC                           >  NB501016:230:HFVJLAFXY:4:21503:26583:10081/1‑151 (MQ=60)
                                                                                                                               TTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTAC                              <  NB501016:230:HFVJLAFXY:4:11401:21350:8359/147‑1 (MQ=60)
                                                                                                                                TTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACC                          <  NB501016:230:HFVJLAFXY:2:21305:18620:4649/150‑1 (MQ=60)
                                                                                                                                  ATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTT                        <  NB501016:230:HFVJLAFXY:4:21404:16569:14469/150‑1 (MQ=60)
                                                                                                                                    GTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTT                     >  NB501016:230:HFVJLAFXY:2:21107:1130:9141/1‑151 (MQ=60)
                                                                                                                                    GTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTT                     <  NB501016:230:HFVJLAFXY:3:11407:13879:12756/151‑1 (MQ=60)
                                                                                                                                    GTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTT                     <  NB501016:230:HFVJLAFXY:3:11408:17850:2656/151‑1 (MQ=60)
                                                                                                                                    GTTCATGGATATTTGTAACCCATCGGAAAACTCCTGCTATAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGAAGTTTCTATAATATGCGTGTTTCTTGAGAATTTAACAATTACAACCTTGTT                     >  NB501016:230:HFVJLAFXY:2:21209:22057:3243/1‑151 (MQ=60)
                                                                                                                                      TCATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTA                    <  NB501016:230:HFVJLAFXY:4:21607:1256:9285/150‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTATCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAG                  <  NB501016:230:HFVJLAFXY:4:21609:2536:2106/151‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAG                  <  NB501016:230:HFVJLAFXY:1:11303:9795:19928/151‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAG                  <  NB501016:230:HFVJLAFXY:4:21412:26399:11098/151‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAG                  <  NB501016:230:HFVJLAFXY:4:21406:19634:3050/151‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAG                  <  NB501016:230:HFVJLAFXY:3:11503:8773:17597/151‑1 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTA                    >  NB501016:230:HFVJLAFXY:1:11210:21564:13229/1‑149 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTA                    >  NB501016:230:HFVJLAFXY:1:11306:6049:11595/1‑149 (MQ=60)
                                                                                                                                       CATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACATATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAAC                                    >  NB501016:230:HFVJLAFXY:2:21306:23433:9153/1‑133 (MQ=60)
                                                                                                                                        ATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGT                 >  NB501016:230:HFVJLAFXY:4:21603:22660:11309/1‑151 (MQ=60)
                                                                                                                                        ATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGT                 <  NB501016:230:HFVJLAFXY:2:21311:9402:17819/151‑1 (MQ=60)
                                                                                                                                        ATGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGT                 >  NB501016:230:HFVJLAFXY:4:21601:17251:6356/1‑151 (MQ=60)
                                                                                                                                         TGGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTC                >  NB501016:230:HFVJLAFXY:3:11506:3779:15000/1‑151 (MQ=60)
                                                                                                                                          GGATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTC                <  NB501016:230:HFVJLAFXY:1:21207:18895:15680/150‑1 (MQ=60)
                                                                                                                                            ATATTTTTAACCCTTCGGAAAACTCCTTCTTTAGCAATATTTTCGCTGTATTGCTGAAATTGGATTTCTCTTGATTTCAACCAATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACTTTTACAACCTTTTTAAGTCCTT             <  NB501016:230:HFVJLAFXY:3:21412:12428:5886/151‑1 (MQ=60)
                                                                                                                                            ATATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTT             >  NB501016:230:HFVJLAFXY:2:21205:2991:3004/1‑151 (MQ=60)
                                                                                                                                             TATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTG                                                                                                            >  NB501016:230:HFVJLAFXY:4:11505:18064:1584/1‑55 (MQ=60)
                                                                                                                                              ATTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTT            <  NB501016:230:HFVJLAFXY:3:11502:5688:9748/150‑1 (MQ=60)
                                                                                                                                               TTTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTT           >  NB501016:230:HFVJLAFXY:3:21401:22036:8525/1‑150 (MQ=60)
                                                                                                                                                TTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTA          <  NB501016:230:HFVJLAFXY:4:21611:4028:5323/150‑1 (MQ=60)
                                                                                                                                                TTGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTCTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTA          <  NB501016:230:HFVJLAFXY:1:21310:7623:16528/150‑1 (MQ=60)
                                                                                                                                                 TGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT        >  NB501016:230:HFVJLAFXY:4:11511:10001:20173/1‑151 (MQ=60)
                                                                                                                                                 TGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT        >  NB501016:230:HFVJLAFXY:1:21212:16449:2585/1‑151 (MQ=60)
                                                                                                                                                 TGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTAC                              >  NB501016:230:HFVJLAFXY:4:11406:16440:17422/1‑129 (MQ=60)
                                                                                                                                                 TGTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTGTCTTGAGAATTTAACATTTAGAACCTTTTTAAGTCCTTTTATT        >  NB501016:230:HFVJLAFXY:3:21410:5421:1232/1‑151 (MQ=60)
                                                                                                                                                  GTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTA       <  NB501016:230:HFVJLAFXY:3:11608:16654:7454/151‑1 (MQ=60)
                                                                                                                                                  GTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT        <  NB501016:230:HFVJLAFXY:1:21301:24517:14030/150‑1 (MQ=60)
                                                                                                                                                  GTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATT        >  NB501016:230:HFVJLAFXY:1:11102:26579:18367/1‑150 (MQ=60)
                                                                                                                                                  GTAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTAAAACTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTA       <  NB501016:230:HFVJLAFXY:3:21601:25581:9693/151‑1 (MQ=60)
                                                                                                                                                   TAACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTA       >  NB501016:230:HFVJLAFXY:3:21503:11251:3813/1‑150 (MQ=60)
                                                                                                                                                    AACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAAC     >  NB501016:230:HFVJLAFXY:2:11303:18877:6252/1‑151 (MQ=60)
                                                                                                                                                    AACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAA      <  NB501016:230:HFVJLAFXY:3:11601:21731:7177/150‑1 (MQ=60)
                                                                                                                                                     ACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACA    <  NB501016:230:HFVJLAFXY:1:11201:4717:8183/151‑1 (MQ=60)
                                                                                                                                                     ACCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAAC     >  NB501016:230:HFVJLAFXY:3:21611:10465:20130/1‑150 (MQ=60)
                                                                                                                                                      CCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATTCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACAC   >  NB501016:230:HFVJLAFXY:1:21206:2114:10702/1‑151 (MQ=60)
                                                                                                                                                      CCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACAC   <  NB501016:230:HFVJLAFXY:1:11103:5459:9256/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                       
ATTATGCCAACACCCCGGTGCTGACACGGAAGAAACCGGACGTTATGATTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTATCAAAGGTATAGTAATATCTTTTATGTTCGTGGATATTTGTAATCCATCGGAAAACTCCTGCTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCATAGGACGTTTCTATAAGATGCGTATTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTCCTTTTATTAACACG  >  NC_000913/579135‑579436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑