Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 579,671 T→C 100% intergenic (‑77/‑209) ybcV ← / → ybcW DLP12 prophage; uncharacterized protein/DLP12 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,6710TC98.9% 12753.2 / 28.4 4453intergenic (‑77/‑209)ybcV/ybcWDLP12 prophage; uncharacterized protein/DLP12 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base C (2024/2339);  total (2073/2380)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 4661 total aligned reads displayed.

TAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCA  >  NC_000913/579524‑579816
                                                                                                                                                   |                                                                                                                                                 
 aaaaCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCTTGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCctt                                                                                                                                                 >  7:225082/1‑149 (MQ=255)
  aaaCAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCttt                                                                                                                                                 <  3:148931/148‑1 (MQ=255)
  aaaCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCGATTGGTAACACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCctt                                                                                                                                                 <  6:354610/148‑1 (MQ=255)
  aaaCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCctt                                                                                                                                                 <  7:288583/148‑1 (MQ=255)
  aaaCAATTCACAGTCTAAATCTGTTCGCACTTGATCGAAGATTTCTTTAAAAATGGCAACCTGAGCCATTGGTATCACCTTCGATGTGAGGCGAGGGCGCGTAGTTGGCGTTATCGTTTTTATCGGTTCAATCTGGTCTGACCTCCTtg                                                                                                                                                >  6:89480/1‑149 (MQ=255)
   aaCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTtgt                                                                                                                                               >  8:189000/1‑149 (MQ=255)
   aaCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTtgt                                                                                                                                               <  2:316227/149‑1 (MQ=255)
     cAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTgtgt                                                                                                                                             >  2:396589/1‑149 (MQ=255)
      aaTTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGtt                                                                                                                                            <  8:159077/149‑1 (MQ=255)
      aaTTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGtt                                                                                                                                            <  8:306544/149‑1 (MQ=255)
      aaTTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGtt                                                                                                                                            <  2:204575/149‑1 (MQ=255)
       aTTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGttt                                                                                                                                           <  8:383838/149‑1 (MQ=255)
          cacaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttg                                                                                                                                         <  7:334673/148‑1 (MQ=255)
           acaGTCTAACTCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGACATTTGTAAAACGTTCAATGTGATACTAGGGCTAGTAGTTTGCATTATCTTTTTTATAGTTTCAATCTGGTCTGACCTAATTGTGTTttgtt                                                                                                                                       >  3:260970/1‑149 (MQ=255)
           acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttgtt                                                                                                                                       <  5:323257/149‑1 (MQ=255)
           acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttgtt                                                                                                                                       >  6:244739/1‑149 (MQ=255)
           acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttgtt                                                                                                                                       >  5:290810/1‑149 (MQ=255)
           acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttgt                                                                                                                                        <  4:160204/148‑1 (MQ=255)
           acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTttggt                                                                                                                                       >  5:190285/1‑147 (MQ=255)
             aGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTtga                                                                                                                                     <  3:325495/149‑1 (MQ=255)
                ctAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTtgatga                                                                                                                                  >  4:368919/2‑149 (MQ=255)
                  aaaTCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGAtt                                                                                                                                >  3:178215/1‑149 (MQ=255)
                   aaTCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGATGATGAtt                                                                                                                                >  4:85725/1‑148 (MQ=255)
                     tCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTAt                                                                                                                             >  2:23626/1‑149 (MQ=255)
                            gCACTTTCTCGAATATTTAGTTAAATATGTGAACCTGAGCCATTGGTAAAACATTACATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTTGTCTGCCCTCCTTGTGTTTTGTTGATGATTTATGTCAAat                                                                                                                      <  6:216205/149‑1 (MQ=255)
                              aCTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGCGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATGTATGTCAAatat                                                                                                                    >  8:275915/1‑149 (MQ=255)
                                  gATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTa                                                                                                                  <  7:258187/147‑1 (MQ=255)
                                  gATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTa                                                                                                                  <  7:386697/147‑1 (MQ=255)
                                  gATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAg                                                                                                                 >  8:105970/1‑148 (MQ=255)
                                   aTCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAgg                                                                                                                <  1:100192/148‑1 (MQ=255)
                                    tCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGaa                                                                                                              >  1:191136/1‑149 (MQ=255)
                                      gAATATTTCTTTAAAAATGGCACCCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATg                                                                                                            >  1:55723/1‑149 (MQ=255)
                                      gAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTa                                                                                                                              <  2:177478/131‑1 (MQ=255)
                                           tttCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGtttt                                                                                                        <  5:55021/148‑1 (MQ=255)
                                           tttCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTc                                                                                                       <  2:262830/149‑1 (MQ=255)
                                            ttCTTTAAAAATGGCAACCTGAGCCATTGGTAACACCTTCCATTTGATACGAGGGCGCGGAGTTTGAATTATAGTTTTTATAGTTTCAATCTGGTCTGACATCATTGGGTTTTGTTGATGAGTTATGTCAAATATTAGGAATGTTTTCa                                                                                                      >  5:109797/1‑149 (MQ=255)
                                            atCTTTAAAAATGGCAACCAGAGCCATAGGTAAAACCTTCCATGAGATACGAGCGCGCGAACATTGCATTATGGTTTAAATCGATTCAATCTCGTCAGACCTCCATGAGTTTAGTTGATGATTTATGTCAAATATTAGGAATGTTTTc                                                                                                       >  6:189677/2‑148 (MQ=255)
                                              cTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCac                                                                                                     <  4:215744/148‑2 (MQ=255)
                                               tttAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCact                                                                                                    >  1:277562/1‑146 (MQ=255)
                                               tttAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGCGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGACTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCact                                                                                                    >  1:248213/1‑146 (MQ=255)
                                                  aaaaaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTaa                                                                                                 <  5:350368/148‑1 (MQ=255)
                                                   aaaaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATa                                                                                               <  6:159748/149‑1 (MQ=255)
                                                   aaaaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATa                                                                                               <  6:385162/149‑1 (MQ=255)
                                                     aaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGAACTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGt                                                                                             <  6:63732/149‑1 (MQ=255)
                                                         gCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTg                                                                                         <  7:143656/149‑1 (MQ=255)
                                                          cAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTgg                                                                                        <  8:22507/149‑1 (MQ=255)
                                                           aaCCTGAGCCATTTGTAAAACCTTCCTTGTGATACGAGGGATCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTgg                                                                                        <  7:81339/148‑1 (MQ=255)
                                                            aCCTGAGCCATTGGTAAAACCATCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGGCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTAAATTAATAGTATTGGtt                                                                                      >  3:45843/1‑149 (MQ=255)
                                                             ccTGAGCCATTGGTAAAACCTTCCATGTGCTACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTg                                                                                     >  6:332549/1‑149 (MQ=255)
                                                             ccTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTg                                                                                     >  8:330751/1‑149 (MQ=255)
                                                             ccTGAGCAATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTg                                                                                     <  5:221244/149‑1 (MQ=255)
                                                              cTGAGCCATTGGTAAAACCTTCCATGTGATACTAGGTCTCGTATGTTGCATTATCTTTTTTATCGTTTCAATCTTGTCTGACCTCCTTTTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGc                                                                                    <  5:258691/149‑1 (MQ=255)
                                                                   ccATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTaa                                                                                >  6:10877/1‑148 (MQ=255)
                                                                     aTTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACa                                                                              >  5:382792/1‑148 (MQ=255)
                                                                         gTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATCTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTg                                                                         >  8:358628/1‑149 (MQ=255)
                                                                          tAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGc                                                                        >  2:339507/1‑149 (MQ=255)
                                                                            aaaCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCgg                                                                      >  1:35628/1‑149 (MQ=255)
                                                                              aCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTc                                                                    <  7:192415/149‑1 (MQ=255)
                                                                               ccTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCg                                                                       >  3:231081/1‑145 (MQ=255)
                                                                                      tgtgATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCg                                                                       >  5:160998/1‑138 (MQ=255)
                                                                                       gtgATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCa                                                           >  3:68980/1‑149 (MQ=255)
                                                                                        tgATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCa                                                           <  4:339598/148‑1 (MQ=255)
                                                                                          aTACGAGTGCGCGTAGTTTGCATTATCGTTTTTATCTTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTc                                                        <  1:165758/149‑1 (MQ=255)
                                                                                           tACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCt                                                       <  7:356773/149‑1 (MQ=255)
                                                                                             cGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTg                                                      <  3:96912/148‑1 (MQ=255)
                                                                                             cGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTg                                                      <  1:380283/148‑1 (MQ=255)
                                                                                               aGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAg                                                   <  2:150448/149‑1 (MQ=255)
                                                                                                gggCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTg                                                      <  7:313218/145‑1 (MQ=255)
                                                                                                gggCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAgg                                                  <  1:32961/149‑1 (MQ=255)
                                                                                                 ggCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAggg                                                 <  2:9369/149‑1 (MQ=255)
                                                                                                   cgcgTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTc                                                                    <  8:252370/128‑1 (MQ=255)
                                                                                                      gTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATa                                            >  5:132027/1‑149 (MQ=255)
                                                                                                       tAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATAc                                           <  3:127062/149‑1 (MQ=255)
                                                                                                          tttGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAc                                        >  4:309657/1‑149 (MQ=255)
                                                                                                          tttGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAc                                        <  6:277836/149‑1 (MQ=255)
                                                                                                           ttGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAc                                        <  8:74115/148‑1 (MQ=255)
                                                                                                           ttGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAc                                        <  3:353379/148‑1 (MQ=255)
                                                                                                            tGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAcc                                       <  7:116730/148‑1 (MQ=255)
                                                                                                            tGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAcc                                       >  1:30145/1‑148 (MQ=255)
                                                                                                             gCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTg                                                                                     <  8:132682/101‑1 (MQ=255)
                                                                                                              cATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGa                                     <  7:168209/148‑1 (MQ=255)
                                                                                                              cATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAc                                    <  4:28020/149‑1 (MQ=255)
                                                                                                                ttatcgtttttatcgtttCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGAGTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGa                                     >  4:58560/1‑146 (MQ=255)
                                                                                                                     ggttttATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGatgta                             <  2:368125/147‑1 (MQ=255)
                                                                                                                     gTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCGTTCTGGAGGGAAATACAACCGACAGatgt                              <  5:79722/148‑1 (MQ=255)
                                                                                                                     gTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGatgta                             <  1:222250/149‑1 (MQ=255)
                                                                                                                      tttttATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGatgtat                            <  2:67138/149‑1 (MQ=255)
                                                                                                                           aTCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAg                       >  7:59476/1‑149 (MQ=255)
                                                                                                                              gtttCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGcc                    <  3:199773/147‑1 (MQ=255)
                                                                                                                              gtttCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACACATGTATTTAAGGc                     >  4:192005/3‑148 (MQ=255)
                                                                                                                               tttCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGcc                    >  1:78862/2‑148 (MQ=255)
                                                                                                                                ttCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCaa                  >  2:28815/1‑149 (MQ=255)
                                                                                                                                 tCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAAc                 >  6:134418/1‑149 (MQ=255)
                                                                                                                                 tCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAAc                 >  1:134129/1‑149 (MQ=255)
                                                                                                                                         gTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGc             <  4:248708/145‑1 (MQ=255)
                                                                                                                                         gTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCa          >  3:295077/1‑148 (MQ=255)
                                                                                                                                         gTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCa          >  2:116042/1‑148 (MQ=255)
                                                                                                                                              aCCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCtt    >  4:13124/1‑149 (MQ=255)
                                                                                                                                                ctccTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTc   >  8:49286/1‑148 (MQ=255)
                                                                                                                                                ctccTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCa  <  6:30780/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCA  >  NC_000913/579524‑579816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 2559 total aligned reads displayed.
GAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAG  >  NC_000913/579521‑579821
                                                                                                                                                      |                                                                                                                                                      
GAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCC                                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:11109:15773:15984/1‑151 (MQ=60)
   TAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     >  NB501016:230:HFVJLAFXY:2:11201:26565:16458/1‑151 (MQ=60)
    AAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCACCCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGT                                                                                                                                                    <  NB501016:230:HFVJLAFXY:1:21207:24467:10051/151‑1 (MQ=60)
    AAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGT                                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11610:22331:13478/151‑1 (MQ=60)
    AAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:2:11203:21470:12407/150‑1 (MQ=60)
    AAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:1:21212:14334:14730/150‑1 (MQ=60)
    AAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:21503:4677:13684/150‑1 (MQ=60)
     AAACAATTCACAGTCTAAATCTTTTCGCTCTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTG                                                                                                                                                   <  NB501016:230:HFVJLAFXY:2:21107:3106:18112/151‑1 (MQ=60)
     AAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCGTTGGTAAAACCTTCCATGTGATACGAGGGCGAGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:1:21203:8630:9546/1‑151 (MQ=60)
       ACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTT                                                                                                                                                 >  NB501016:230:HFVJLAFXY:1:11310:13414:19468/1‑151 (MQ=60)
        CAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTT                                                                                                                                                <  NB501016:230:HFVJLAFXY:3:11612:25733:9398/151‑1 (MQ=60)
         AATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTT                                                                                                                                               >  NB501016:230:HFVJLAFXY:1:21308:3440:17079/1‑151 (MQ=60)
          ATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTG                                                                                                                                              <  NB501016:230:HFVJLAFXY:4:11403:10623:5145/151‑1 (MQ=60)
           gaccattttgcgcaggtcacctttatctgccgccactcataaAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGT                                                                                                                                             <  NB501016:230:HFVJLAFXY:2:21306:17834:3406/109‑1 (MQ=60)
             CACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTG                                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21602:14184:5117/151‑1 (MQ=60)
             CACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTG                                                                                                                                           >  NB501016:230:HFVJLAFXY:3:21508:11697:4952/1‑151 (MQ=60)
             CACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTT                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11512:1754:8410/150‑1 (MQ=60)
                AGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21301:26312:12751/151‑1 (MQ=60)
                AGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATG                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:21201:15506:17598/1‑151 (MQ=60)
                AGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21212:18439:12377/151‑1 (MQ=60)
                 GTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTACATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGCCCTCCTTGTGTTTTGTTGATG                                                                                                                                        >  NB501016:230:HFVJLAFXY:3:11408:2894:15925/1‑150 (MQ=60)
                   CTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCTTTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATT                                                                                                                                     >  NB501016:230:HFVJLAFXY:3:21506:1539:11589/1‑151 (MQ=60)
                    TAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTT                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11111:16105:8299/1‑151 (MQ=60)
                    TAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTT                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11407:5629:6011/1‑151 (MQ=60)
                     AAATCTTTTCGCACTTGATCGAATATTTCTTTATAACTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTA                                                                                                                                   <  NB501016:230:HFVJLAFXY:2:11106:24617:2529/151‑1 (MQ=60)
                     AAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCTTTGGTAAAACCTTCCATGTTATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGAT                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:21101:13936:19295/148‑1 (MQ=60)
                     AAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTT                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11604:7645:17478/1‑150 (MQ=60)
                     AAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGAT                                                                                                                                      >  NB501016:230:HFVJLAFXY:2:21101:9882:17269/1‑148 (MQ=60)
                     AAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTG                                                                                                                                              <  NB501016:230:HFVJLAFXY:1:11108:17835:6775/140‑1 (MQ=60)
                     AAATCTTATAGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTCAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGAGTAGTATGCAATATCGTTTTTATCGTTTCAATCTCGTCTGACCTCATTGTGTTTTGTTGATTATTTT                                                                                                                                   >  NB501016:230:HFVJLAFXY:1:21106:2376:16537/1‑151 (MQ=60)
                      AATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTAT                                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11109:10975:15873/151‑1 (MQ=60)
                        TCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCC                                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21109:16217:19744/127‑1 (MQ=60)
                         CTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCACGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTC                                                                                                                               >  NB501016:230:HFVJLAFXY:4:11610:16405:7445/1‑151 (MQ=60)
                           TTTCTCACTTGATCGAATATTTCTTTATAAATGGCAACCTGAGCCATTGGTAAATCCTTTCATGGGATACGAGGGCGCGTAGTTTGCATTATGGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTC                                                                                                                               <  NB501016:230:HFVJLAFXY:2:21204:3077:6645/149‑1 (MQ=60)
                           TTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATG                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21401:20819:5527/147‑1 (MQ=60)
                            TTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAA                                                                                                                             >  NB501016:230:HFVJLAFXY:3:21612:15189:19516/1‑150 (MQ=60)
                              CGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATA                                                                                                                          <  NB501016:230:HFVJLAFXY:1:21105:17709:19280/151‑1 (MQ=60)
                              CGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTACCATGTGATACGAGGGAGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGTTCTGACATCCTTGTGTTTTGTTGAGGATTAATGTCAAAT                                                                                                                           >  NB501016:230:HFVJLAFXY:1:11204:22192:2101/1‑150 (MQ=60)
                              CGCACTTGATCGAATATTTCTTTAAAAATGGCAACATGAGCCATTTGTACAACCTTCCATGTGATCCGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAAT                                                                                                                           <  NB501016:230:HFVJLAFXY:3:11508:25135:4081/150‑1 (MQ=60)
                                 ACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTA                                                                                                                       <  NB501016:230:HFVJLAFXY:4:11607:13241:19970/151‑1 (MQ=60)
                                 ACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATT                                                                                                                                     >  NB501016:230:HFVJLAFXY:3:21402:15560:10486/1‑137 (MQ=60)
                                  CTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATT                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21201:20497:4244/1‑149 (MQ=60)
                                  CATGATCGAATATTTCTTTAAAAATGGAAAACTGATCCATTTGTAAAACGTTCCATGTGATACGAGTGCGCGTTGTTTGGATTATAGTTTTAATCGTTTCAATCTTGTCTGACCTAGTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:4:21610:10284:4352/151‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAATACTGGCAACCTGAGCCATTGGTATAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCTTTATCTTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:3:11605:26758:5809/149‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGG                                                                                                                     >  NB501016:230:HFVJLAFXY:1:21206:18335:14118/1‑150 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11312:9021:13842/149‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:3:21411:24983:15930/149‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11608:19430:6914/149‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGGGTTTTGTTGATGATTTATGTCAAATATTAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:21312:18969:20354/150‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGGTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:2:21111:17255:7303/149‑1 (MQ=60)
                                    TGATCGAATATTTCTTTAAAAATGGCAACCTGAGCAATTTGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:3:21509:2408:17323/149‑1 (MQ=60)
                                     GATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGA                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11606:24005:9280/150‑1 (MQ=60)
                                      ATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAAT                                                                                                                  >  NB501016:230:HFVJLAFXY:3:11404:23462:10840/1‑151 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGT                                                                                                                >  NB501016:230:HFVJLAFXY:1:11206:7590:11317/1‑151 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGT                                                                                                                >  NB501016:230:HFVJLAFXY:2:11205:17823:7112/1‑151 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGT                                                                                                                >  NB501016:230:HFVJLAFXY:1:11302:23638:17675/1‑151 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGT                                                                                                                >  NB501016:230:HFVJLAFXY:4:21510:6197:15491/1‑151 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATG                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11502:8134:13657/150‑1 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTAT                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:11308:7634:20162/1‑133 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     >  NB501016:230:HFVJLAFXY:4:21606:5005:14430/1‑114 (MQ=60)
                                        CGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCCTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTAAGTCAAATATTAGGAATGT                                                                                                                >  NB501016:230:HFVJLAFXY:1:11211:1683:11685/1‑151 (MQ=60)
                                         GAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTT                                                                                                               <  NB501016:230:HFVJLAFXY:4:11506:6427:15926/151‑1 (MQ=60)
                                           ATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTT                                                                                                             <  NB501016:230:HFVJLAFXY:2:11112:19813:10600/151‑1 (MQ=60)
                                            TATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTC                                                                                                            >  NB501016:230:HFVJLAFXY:2:21203:14619:5818/1‑151 (MQ=60)
                                             ATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTC                                                                                                            <  NB501016:230:HFVJLAFXY:2:11310:14637:20350/150‑1 (MQ=60)
                                             ATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTC                                                                                                            <  NB501016:230:HFVJLAFXY:2:11107:4243:15514/150‑1 (MQ=60)
                                             ATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      >  NB501016:230:HFVJLAFXY:2:21309:22016:8842/1‑140 (MQ=60)
                                             ATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTC                                                                                                            <  NB501016:230:HFVJLAFXY:1:11307:21799:18789/150‑1 (MQ=60)
                                              TTTCTTTAAAAATGGCAAGCTGATCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGGTTTTATCTTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCA                                                                                                           <  NB501016:230:HFVJLAFXY:2:21210:15281:3831/150‑1 (MQ=60)
                                              TTTCTTTAAAAATGGCAACCTGAGCCATTGGTAACACCTTCCATTTGATACGAGGGCGCGGAGTTTGAATTATAGTTTTTATAGTTTCAATCTGGTCTGACATCATTGGGTTTTGTTGATGAGTTATGTCAAATATTAGGAATGTTTTCAC                                                                                                          >  NB501016:230:HFVJLAFXY:3:11509:3389:18995/1‑151 (MQ=60)
                                              TTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATG                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11602:23787:7522/1‑128 (MQ=60)
                                               TTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACT                                                                                                         >  NB501016:230:HFVJLAFXY:2:11303:18143:14402/1‑151 (MQ=60)
                                                TCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACT                                                                                                         <  NB501016:230:HFVJLAFXY:1:11201:23476:7922/150‑1 (MQ=60)
                                                 CTTTAAAAATGGCAACCTGAGCCATTGGTAAACCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGCCCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTC                                                                                                            >  NB501016:230:HFVJLAFXY:4:21402:25536:19793/1‑146 (MQ=60)
                                                 CTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTGAATCTGGTCTGCCCTCCTTGTGTTTTGTTGATG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:21605:19179:11438/1‑118 (MQ=60)
                                                 CTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTT                                                                                                        >  NB501016:230:HFVJLAFXY:3:11609:1119:9082/1‑150 (MQ=60)
                                                 CTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTT                                                                                                        >  NB501016:230:HFVJLAFXY:1:21202:9103:6544/1‑150 (MQ=60)
                                                 CTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATG                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11409:10269:9712/125‑1 (MQ=60)
                                                  TTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTA                                                                                                       >  NB501016:230:HFVJLAFXY:4:11509:26369:16815/1‑150 (MQ=60)
                                                   TTAAAAATGGCAACCTGAGCCATTTGTAAAACCTTCCATGTGATACGAGGGCTCGTAGTTTGCATTATCTTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTA                                                                                                       <  NB501016:230:HFVJLAFXY:3:11402:15222:7422/149‑1 (MQ=60)
                                                   TTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTT                                                                                                        <  NB501016:230:HFVJLAFXY:3:11511:20151:15892/148‑1 (MQ=60)
                                                    TAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAAT                                                                                                     <  NB501016:230:HFVJLAFXY:3:21606:12024:18837/150‑1 (MQ=60)
                                                    TAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAAT                                                                                                     <  NB501016:230:HFVJLAFXY:3:21408:13403:5427/150‑1 (MQ=60)
                                                    TAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAAT                                                                                                     <  NB501016:230:HFVJLAFXY:2:11309:10639:17516/150‑1 (MQ=60)
                                                     ATAAATGGCAACCTGAGCCATTGGTAAAAGATTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCTTTTCAATCTGGTCTGACCTCCTTGTTTTTTGTTTATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAG                                                                                                   <  NB501016:230:HFVJLAFXY:1:11305:20348:1875/151‑1 (MQ=60)
                                                     AAAAATGGCAACCTTAGCCATTTGTAAAAACTTCCATGTGATACGAGTGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAG                                                                                                   <  NB501016:230:HFVJLAFXY:1:11104:1655:2646/151‑1 (MQ=60)
                                                     AAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAG                                                                                                   <  NB501016:230:HFVJLAFXY:3:11406:6318:7943/151‑1 (MQ=60)
                                                      AAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAG                                                                                                   <  NB501016:230:HFVJLAFXY:1:11202:22364:2000/150‑1 (MQ=60)
                                                         ATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATT                                                                                               <  NB501016:230:HFVJLAFXY:1:21103:8255:15274/151‑1 (MQ=60)
                                                           GGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGG                                                                                             <  NB501016:230:HFVJLAFXY:3:21405:11963:1548/151‑1 (MQ=60)
                                                           GGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTG                                                                                              <  NB501016:230:HFVJLAFXY:2:21312:17827:12813/150‑1 (MQ=60)
                                                            GCAACCTTAGCCATTGTTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGT                                                                                            <  NB501016:230:HFVJLAFXY:3:11501:10288:20053/151‑1 (MQ=60)
                                                             CAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTTCATTATAGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGG                                                                                             <  NB501016:230:HFVJLAFXY:3:11412:12754:10247/149‑1 (MQ=60)
                                                              AACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTG                                                                                          <  NB501016:230:HFVJLAFXY:1:11102:14600:17484/151‑1 (MQ=60)
                                                                CCTGCGCCATTGGTCAAACCTTCCAGGTGATACTAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGCATGTTTTCACTTAATAGTATTGGTTGCG                                                                                        <  NB501016:230:HFVJLAFXY:3:11403:25164:5270/151‑1 (MQ=60)
                                                                CCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCG                                                                                        <  NB501016:230:HFVJLAFXY:1:21210:22911:8274/151‑1 (MQ=60)
                                                                  TGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTA                                                                                                                       >  NB501016:230:HFVJLAFXY:4:11603:21017:7527/1‑118 (MQ=60)
                                                                   GAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGAGTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTA                                                                                      <  NB501016:230:HFVJLAFXY:3:21502:25688:9029/150‑1 (MQ=60)
                                                                    AGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAAC                                                                                    <  NB501016:230:HFVJLAFXY:2:21303:10254:17028/151‑1 (MQ=60)
                                                                     GCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACA                                                                                   <  NB501016:230:HFVJLAFXY:2:21311:12927:7276/151‑1 (MQ=60)
                                                                     GCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACA                                                                                   <  NB501016:230:HFVJLAFXY:4:11412:1484:6558/151‑1 (MQ=60)
                                                                     GCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAAC                                                                                    >  NB501016:230:HFVJLAFXY:4:21611:6843:5677/1‑150 (MQ=60)
                                                                       CATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAA                                                                                  >  NB501016:230:HFVJLAFXY:3:11602:6145:16748/1‑150 (MQ=60)
                                                                       CATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAA                                                                                  >  NB501016:230:HFVJLAFXY:1:21303:2786:18077/1‑150 (MQ=60)
                                                                       CATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11412:9690:13596/83‑1 (MQ=60)
                                                                        ATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAG                                                                                <  NB501016:230:HFVJLAFXY:4:21507:5629:19721/151‑1 (MQ=60)
                                                                            GTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGCCCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCG                                                                            >  NB501016:230:HFVJLAFXY:3:11411:10972:8903/1‑151 (MQ=60)
                                                                            GTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCG                                                                            >  NB501016:230:HFVJLAFXY:2:11211:4826:9923/1‑151 (MQ=60)
                                                                              AAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTTGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGT                                                                          <  NB501016:230:HFVJLAFXY:3:11506:5124:17546/151‑1 (MQ=60)
                                                                              AAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGAGCTCCTTGTGTTTTGTTGTTGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGG                                                                           <  NB501016:230:HFVJLAFXY:3:11607:19359:4623/150‑1 (MQ=60)
                                                                              AAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGT                                                                          <  NB501016:230:HFVJLAFXY:2:11105:11624:9293/151‑1 (MQ=60)
                                                                              AAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGT                                                                          <  NB501016:230:HFVJLAFXY:2:21208:14999:6322/151‑1 (MQ=60)
                                                                              AAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGGCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTGGCGTAACAAAGTGCGG                                                                           <  NB501016:230:HFVJLAFXY:1:21205:15291:14654/150‑1 (MQ=60)
                                                                               AAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGC                                                                             <  NB501016:230:HFVJLAFXY:4:11504:18213:13217/147‑1 (MQ=60)
                                                                                AACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGGCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCC                                                                        <  NB501016:230:HFVJLAFXY:4:21506:23817:6029/151‑1 (MQ=60)
                                                                                     TCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTG                                                                   >  NB501016:230:HFVJLAFXY:1:21301:11744:17606/1‑151 (MQ=60)
                                                                                      CCATGTGATACGAGGGCGCGTTGTTTGCATTATCGTTTTTATCGTTTCAATCTTGTATGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGGCCTGCTGG                                                                  <  NB501016:230:HFVJLAFXY:1:21107:4577:6383/151‑1 (MQ=60)
                                                                                      CCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGCCCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGG                                                                  >  NB501016:230:HFVJLAFXY:1:21110:3428:16142/1‑151 (MQ=60)
                                                                                      CCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11111:17205:17878/99‑1 (MQ=60)
                                                                                       CATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATATTATTGGTTGCTTAACAAAGTtcgttccttctga                                                                  >  NB501016:230:HFVJLAFXY:3:21512:16017:1699/1‑137 (MQ=60)
                                                                                        ATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTG                                                                                              >  NB501016:230:HFVJLAFXY:3:11604:11019:5330/1‑121 (MQ=60)
                                                                                          GTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATT                                                              >  NB501016:230:HFVJLAFXY:2:21304:21057:17604/1‑151 (MQ=60)
                                                                                           TGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTC                                                             >  NB501016:230:HFVJLAFXY:4:21407:14102:5248/1‑151 (MQ=60)
                                                                                            GATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTTGTTGCTTAACAAAGTGCGGTCCTGCTGGCATTCT                                                            >  NB501016:230:HFVJLAFXY:2:21105:7685:12740/1‑151 (MQ=60)
                                                                                            GATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTC                                                             <  NB501016:230:HFVJLAFXY:3:21504:24703:19135/150‑1 (MQ=60)
                                                                                             ATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTG                                                           <  NB501016:230:HFVJLAFXY:4:21404:20744:2802/151‑1 (MQ=60)
                                                                                             ATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTG                                                           <  NB501016:230:HFVJLAFXY:4:21604:19240:19735/151‑1 (MQ=60)
                                                                                             ATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAG                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11601:15811:3846/1‑92 (MQ=60)
                                                                                               ACGAGGGCGCTTAGTTTGCATTATCGTTTTTATCTTTGCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGG                                                          <  NB501016:230:HFVJLAFXY:3:21603:24485:20122/150‑1 (MQ=60)
                                                                                               ACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTTGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGG                                                          <  NB501016:230:HFVJLAFXY:3:11510:22421:18331/150‑1 (MQ=60)
                                                                                                CGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAG                                                        <  NB501016:230:HFVJLAFXY:2:11208:13800:3703/151‑1 (MQ=60)
                                                                                                CGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAG                                                        <  NB501016:230:HFVJLAFXY:2:21206:21537:18745/151‑1 (MQ=60)
                                                                                                CGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAG                                                        <  NB501016:230:HFVJLAFXY:1:11101:13999:15584/151‑1 (MQ=60)
                                                                                                CGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATATATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAG                                                        <  NB501016:230:HFVJLAFXY:1:11303:23628:11368/151‑1 (MQ=60)
                                                                                                 GAGGGCGAGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGGGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGG                                                       <  NB501016:230:HFVJLAFXY:1:21102:19990:4896/151‑1 (MQ=60)
                                                                                                 GAGGCCTCGTTGTTTGCATTATGGTTTTTATCGTTTGCATCTGGTCTGACGTCCTTGTGTTTTGTGGCTGATTTCTGTCAAATTTTAGGAAGGTTTTGTCTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGGATTCTGGTGG                                                       <  NB501016:230:HFVJLAFXY:4:21608:21785:2869/151‑1 (MQ=56)
                                                                                                  AGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTA                                                                                      >  NB501016:230:HFVJLAFXY:2:11307:21600:20298/1‑119 (MQ=60)
                                                                                                     GCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAA                                                   <  NB501016:230:HFVJLAFXY:4:11510:22951:2088/151‑1 (MQ=60)
                                                                                                      CGCGTAGTTTGCATTATGGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAAT                                                  <  NB501016:230:HFVJLAFXY:1:11307:11642:15071/151‑1 (MQ=60)
                                                                                                      CGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGG                                                       >  NB501016:230:HFVJLAFXY:4:21601:17778:4581/1‑146 (MQ=60)
                                                                                                       GCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATA                                                 <  NB501016:230:HFVJLAFXY:4:11601:22980:14649/151‑1 (MQ=60)
                                                                                                       GCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATA                                                 <  NB501016:230:HFVJLAFXY:4:21511:18121:8209/151‑1 (MQ=60)
                                                                                                        gtTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATAC                                                <  NB501016:230:HFVJLAFXY:4:21508:23603:1650/149‑1 (MQ=60)
                                                                                                        CGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATAC                                                >  NB501016:230:HFVJLAFXY:3:11502:10574:9658/1‑151 (MQ=60)
                                                                                                        CGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATA                                                 >  NB501016:230:HFVJLAFXY:3:21406:4188:15871/1‑150 (MQ=60)
                                                                                                        CGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACA                                                                                   >  NB501016:230:HFVJLAFXY:1:11311:25304:2097/1‑116 (MQ=60)
                                                                                                         GTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGG                                                          >  NB501016:230:HFVJLAFXY:1:21310:20958:8060/1‑140 (MQ=60)
                                                                                                           AGTTTGCATTATCGTTATTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAC                                             <  NB501016:230:HFVJLAFXY:2:11103:6630:4277/151‑1 (MQ=60)
                                                                                                            GTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACC                                            >  NB501016:230:HFVJLAFXY:1:11209:16489:7233/1‑151 (MQ=60)
                                                                                                              TTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCG                                           <  NB501016:230:HFVJLAFXY:1:21302:14971:17599/150‑1 (MQ=60)
                                                                                                              TTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCG                                           <  NB501016:230:HFVJLAFXY:4:11508:4412:18683/150‑1 (MQ=60)
                                                                                                              TTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCG                                           <  NB501016:230:HFVJLAFXY:1:21209:6257:11764/150‑1 (MQ=60)
                                                                                                                GCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAC                                         <  NB501016:230:HFVJLAFXY:4:21505:3488:6925/150‑1 (MQ=60)
                                                                                                                GCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAC                                         <  NB501016:230:HFVJLAFXY:3:11505:15505:16302/150‑1 (MQ=60)
                                                                                                                GCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAC                                         <  NB501016:230:HFVJLAFXY:3:11504:15349:12599/150‑1 (MQ=60)
                                                                                                                 CATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTG                                                                                          <  NB501016:230:HFVJLAFXY:4:21412:8965:11561/100‑1 (MQ=60)
                                                                                                                    TATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTTACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGAT                                     <  NB501016:230:HFVJLAFXY:4:21406:1103:14060/150‑1 (MQ=60)
                                                                                                                    TATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATG                                    >  NB501016:230:HFVJLAFXY:1:11112:8411:4592/1‑151 (MQ=60)
                                                                                                                    TATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATG                                    >  NB501016:230:HFVJLAFXY:4:11606:20907:5468/1‑151 (MQ=60)
                                                                                                                    TATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACC                                            <  NB501016:230:HFVJLAFXY:1:11106:2454:15890/143‑1 (MQ=60)
                                                                                                                     ATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATG                                    <  NB501016:230:HFVJLAFXY:4:21411:5158:12652/150‑1 (MQ=60)
                                                                                                                     ATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGA                                                         >  NB501016:230:HFVJLAFXY:1:21305:13500:14057/1‑129 (MQ=60)
                                                                                                                     ATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAAAAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGT                                   >  NB501016:230:HFVJLAFXY:1:11105:18005:8329/1‑151 (MQ=60)
                                                                                                                     ATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGGTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGTTCCTGCTGGCATTCTGGAGGGAAATACAACAGACAGATGT                                   >  NB501016:230:HFVJLAFXY:3:21607:18912:14647/1‑151 (MQ=60)
                                                                                                                      TCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGT                                   <  NB501016:230:HFVJLAFXY:3:11409:9687:8458/150‑1 (MQ=60)
                                                                                                                       CGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAGTGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTAT                                 <  NB501016:230:HFVJLAFXY:2:11210:11074:16322/151‑1 (MQ=60)
                                                                                                                       CGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCT                                                                    <  NB501016:230:HFVJLAFXY:4:21410:22191:7863/116‑1 (MQ=60)
                                                                                                                        GTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTGGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATG                                >  NB501016:230:HFVJLAFXY:2:21103:15816:11426/1‑151 (MQ=60)
                                                                                                                        GTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATG                                <  NB501016:230:HFVJLAFXY:4:11405:23902:13415/151‑1 (MQ=60)
                                                                                                                        GTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAAT                                                  <  NB501016:230:HFVJLAFXY:4:21510:12869:17293/133‑1 (MQ=60)
                                                                                                                             TATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGG                           >  NB501016:230:HFVJLAFXY:4:11410:6425:19288/1‑151 (MQ=60)
                                                                                                                             TATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAG                            <  NB501016:230:HFVJLAFXY:2:11305:18966:5834/150‑1 (MQ=60)
                                                                                                                             TATCGTTTCAATATTGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAG                            <  NB501016:230:HFVJLAFXY:4:21603:17995:18860/150‑1 (MQ=60)
                                                                                                                              ATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGG                           <  NB501016:230:HFVJLAFXY:1:21111:8509:11014/150‑1 (MQ=60)
                                                                                                                                CGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCC                         >  NB501016:230:HFVJLAFXY:4:21408:20302:3386/1‑150 (MQ=60)
                                                                                                                                 GTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCA                        >  NB501016:230:HFVJLAFXY:3:11407:7331:9638/1‑150 (MQ=60)
                                                                                                                                 GTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAG                                                                                <  NB501016:230:HFVJLAFXY:2:21104:7584:2733/94‑1 (MQ=60)
                                                                                                                                    TCAATCTGGGCTGACCTCCTTGTGTGGTGTTGATGATTTAAGTCAAATATTAGGAATGTCTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCGGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACG                     <  NB501016:230:HFVJLAFXY:2:11304:14124:13753/150‑1 (MQ=60)
                                                                                                                                     CAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTG                   >  NB501016:230:HFVJLAFXY:2:11101:9766:9081/1‑151 (MQ=60)
                                                                                                                                       ATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCT                 >  NB501016:230:HFVJLAFXY:3:21610:1728:9243/1‑151 (MQ=60)
                                                                                                                                       ATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATG                                    <  NB501016:230:HFVJLAFXY:3:21403:23629:11994/132‑1 (MQ=60)
                                                                                                                                       ATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACAGACAGATGTATGTAAGGCCAACGTGCT                 >  NB501016:230:HFVJLAFXY:3:21511:1157:13039/1‑151 (MQ=60)
                                                                                                                                          TGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCA               >  NB501016:230:HFVJLAFXY:2:21110:13012:12979/1‑150 (MQ=60)
                                                                                                                                              CTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAG                                                        >  NB501016:230:HFVJLAFXY:4:11611:2549:9877/1‑105 (MQ=60)
                                                                                                                                               TGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTT         >  NB501016:230:HFVJLAFXY:2:11301:25120:7650/1‑151 (MQ=60)
                                                                                                                                                GTCCTCCTTGTGTTTTGTTGATGATTTATGTCTAATTTTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTT         <  NB501016:230:HFVJLAFXY:3:21609:19019:14083/150‑1 (MQ=60)
                                                                                                                                                GACCTCCTTGTGTTTTGTTGATGATTTATGTCAATTATTAGGAATGTTTTCACTTATTAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTC        <  NB501016:230:HFVJLAFXY:2:11311:26296:6466/151‑1 (MQ=60)
                                                                                                                                                GACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTC        >  NB501016:230:HFVJLAFXY:2:11306:18674:2687/1‑151 (MQ=60)
                                                                                                                                                GACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTC        >  NB501016:230:HFVJLAFXY:2:21307:23815:7479/1‑151 (MQ=60)
                                                                                                                                                GACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCGCAAATCTTC        <  NB501016:230:HFVJLAFXY:4:11502:13419:15976/151‑1 (MQ=60)
                                                                                                                                                 ACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCA       <  NB501016:230:HFVJLAFXY:1:21307:19634:14638/151‑1 (MQ=60)
                                                                                                                                                  CCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCACAGTGCTCAAATCTCCAT      >  NB501016:230:HFVJLAFXY:2:11206:24031:12443/1‑151 (MQ=60)
                                                                                                                                                  CCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGT                                   <  NB501016:230:HFVJLAFXY:1:21306:19649:1924/122‑1 (MQ=60)
                                                                                                                                                  CCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAAAGATGTATGTAAGGCCAACGTGCTCAAATCTTCAT      >  NB501016:230:HFVJLAFXY:1:11212:5085:7353/1‑151 (MQ=60)
                                                                                                                                                   CTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACA                                                                                   <  NB501016:230:HFVJLAFXY:4:11406:9191:6701/73‑1 (MQ=60)
                                                                                                                                                    TCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATAC    >  NB501016:230:HFVJLAFXY:3:21409:8822:5450/1‑151 (MQ=60)
                                                                                                                                                     CCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCTGTAATGCTGGAATTCTGGAGGTAAATACAAACGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATAC    >  NB501016:230:HFVJLAFXY:2:11110:16914:19841/1‑150 (MQ=60)
                                                                                                                                                     CCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACA   <  NB501016:230:HFVJLAFXY:4:11401:5305:7417/151‑1 (MQ=60)
                                                                                                                                                     CCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATAC    >  NB501016:230:HFVJLAFXY:4:11505:6178:19180/1‑150 (MQ=60)
                                                                                                                                                     CCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATAC    <  NB501016:230:HFVJLAFXY:3:21604:7505:15982/150‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                      
GAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAG  >  NC_000913/579521‑579821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑